Dictyophora indusiate(DI)contains numerous bioactive compounds with immunomodulatory properties.However,the functional activities of its protein hydrolysate and their mechanisms of action in the regulation of systemic...Dictyophora indusiate(DI)contains numerous bioactive compounds with immunomodulatory properties.However,the functional activities of its protein hydrolysate and their mechanisms of action in the regulation of systemic and intestinal mucosal immunity remain poorly understood.This study comprehensively evaluated the immunomodulatory potential of D.indusiate peptide hydrolysate(DIPH)using a multi-omics approach.LC-MS/MS analysis showed that DIPH is composed of peptides containing 8 to 20 amino acid residues,with branchedchain amino acids comprising 20.53%of the total,and exhibits primarily hydrophobic properties.In cyclophosphamide-induced immunosuppressed mice,DIPH exhibited broad restorative effects on immunity via three interrelated mechanisms.Firstly,systemic immune function was enhanced,as demonstrated by significant elevations in serum IgG and IgM levels,and an improvement(4.5-fold)in carbon clearance capacity.Secondly,intestinal barrier integrity was strengthened through the upregulation of tight junction proteins,a 24.7%rise in goblet cells,and a 1.11-fold increase in secretory IgA production.Thirdly,DIPH administration modulated gut microbiota composition,significantly enriching beneficial bacteria including Bifidobacterium and Roseburia while reducing Streptococcus,which was accompanied by a 2.1-fold increase in butyrate production.At the molecular level,DIPH exerted its effects through dual regulatory mechanisms:suppression of the TLR4/MyD88/NF-κB pathway and activation of FoxO1-mediated mucosal restoration.These insights collectively establish DIPH as a multi-target edible fungi-derived immunomodulator that uniquely integrates gut microbiota regulation,systemic immune enhancement,and intestinal barrier restoration,highlighting its promising applications in functional food development and microbiome-targeted therapies.展开更多
Dictyophora indusiata is an edible and medicinal fungi with various nutritious,especially higher in carbohydrates.In this work,polysaccharide from Dictyophora indusiate(DIP)was extracted by the hot-water,and four poly...Dictyophora indusiata is an edible and medicinal fungi with various nutritious,especially higher in carbohydrates.In this work,polysaccharide from Dictyophora indusiate(DIP)was extracted by the hot-water,and four polysaccharide fractions(DIP0,DIP1,DIP2 and DIP3)were obtained using cellulose DE-52 column.The objective of this study was to investigate the structure and bifidogenic activity of four polysaccharide fractions from Dictyophora indusiate.Structure of DIP was evaluated by HPLC,FT-IR and SEM,and the results indicated that four polysaccharide fractions from Dictyophora indusiate were consisted of mannose,glucose and galactose with almost the same functional groups.Remarkably,the DIP3 had significant differences in monosaccharide compositions,which additionally contains glucuronic acid.In addition,DIP0 exhibited a three-dimensional network structure formed by crosslinked chains,while DIP1,DIP2 and DIP3 displayed the irregular flake structure.During the periods of fermentation in vitro,a strong increase of Bifidobacterium longum was observed in the presence of DIP0,DIP1 and DIP2.In particular,Bifidobacterium longum produced more acetic acid in the medium with DIP0 as the carbon source during the fermentation,probably due to the fact that DIP0 contained higher mannose and lower glucose.These results suggest that DIP0 promoted the growth of Bifidobacterium longum and its metabolic synthesis of acetic acid,which would provide necessary information for the prebiotic studies of DIP.展开更多
基金supported by the National Key R&D Pro-gram of China(2023YFD1600503)the Key Research and Development Project of Jiangxi Province(20192ACB60008).
文摘Dictyophora indusiate(DI)contains numerous bioactive compounds with immunomodulatory properties.However,the functional activities of its protein hydrolysate and their mechanisms of action in the regulation of systemic and intestinal mucosal immunity remain poorly understood.This study comprehensively evaluated the immunomodulatory potential of D.indusiate peptide hydrolysate(DIPH)using a multi-omics approach.LC-MS/MS analysis showed that DIPH is composed of peptides containing 8 to 20 amino acid residues,with branchedchain amino acids comprising 20.53%of the total,and exhibits primarily hydrophobic properties.In cyclophosphamide-induced immunosuppressed mice,DIPH exhibited broad restorative effects on immunity via three interrelated mechanisms.Firstly,systemic immune function was enhanced,as demonstrated by significant elevations in serum IgG and IgM levels,and an improvement(4.5-fold)in carbon clearance capacity.Secondly,intestinal barrier integrity was strengthened through the upregulation of tight junction proteins,a 24.7%rise in goblet cells,and a 1.11-fold increase in secretory IgA production.Thirdly,DIPH administration modulated gut microbiota composition,significantly enriching beneficial bacteria including Bifidobacterium and Roseburia while reducing Streptococcus,which was accompanied by a 2.1-fold increase in butyrate production.At the molecular level,DIPH exerted its effects through dual regulatory mechanisms:suppression of the TLR4/MyD88/NF-κB pathway and activation of FoxO1-mediated mucosal restoration.These insights collectively establish DIPH as a multi-target edible fungi-derived immunomodulator that uniquely integrates gut microbiota regulation,systemic immune enhancement,and intestinal barrier restoration,highlighting its promising applications in functional food development and microbiome-targeted therapies.
基金supported by Fujian Agriculture and Forestry University(72202200901)Ten Thousand People Plan of China(KRC19007A)Fujian Province Science and Technology Innovation Leading Talent Project(KRC16002A).
文摘Dictyophora indusiata is an edible and medicinal fungi with various nutritious,especially higher in carbohydrates.In this work,polysaccharide from Dictyophora indusiate(DIP)was extracted by the hot-water,and four polysaccharide fractions(DIP0,DIP1,DIP2 and DIP3)were obtained using cellulose DE-52 column.The objective of this study was to investigate the structure and bifidogenic activity of four polysaccharide fractions from Dictyophora indusiate.Structure of DIP was evaluated by HPLC,FT-IR and SEM,and the results indicated that four polysaccharide fractions from Dictyophora indusiate were consisted of mannose,glucose and galactose with almost the same functional groups.Remarkably,the DIP3 had significant differences in monosaccharide compositions,which additionally contains glucuronic acid.In addition,DIP0 exhibited a three-dimensional network structure formed by crosslinked chains,while DIP1,DIP2 and DIP3 displayed the irregular flake structure.During the periods of fermentation in vitro,a strong increase of Bifidobacterium longum was observed in the presence of DIP0,DIP1 and DIP2.In particular,Bifidobacterium longum produced more acetic acid in the medium with DIP0 as the carbon source during the fermentation,probably due to the fact that DIP0 contained higher mannose and lower glucose.These results suggest that DIP0 promoted the growth of Bifidobacterium longum and its metabolic synthesis of acetic acid,which would provide necessary information for the prebiotic studies of DIP.
