In this study, nest spawning was successfully induced by exogenous hormone injections and seawater flow stimulation, and optimum condition for hatching fertilized eggs of burrow fish mudskipper, Boleophthalmus pectini...In this study, nest spawning was successfully induced by exogenous hormone injections and seawater flow stimulation, and optimum condition for hatching fertilized eggs of burrow fish mudskipper, Boleophthalmus pectinirostris, was searched. Apart from spawning inside the nests, females also spawned outside the nests. The percentages of spawned nests were 8.0% to 24.2%. Most eggs were observed adhered to the inner wall of the top half of the nest. Fertilization rates of the nest-spawned eggs varied from 17.3% to 80.8%. Females could spawn after being artificially confined inside the nests with males at ratios of 1:1 or 1:2, but the spawned eggs were not fertilized. Mean hatching rates of artificially fertilized eggs incubated in round plastic buckets were 32.7%-70.6%, and in the net cages, were 4.2%-20.5%, respectively. Mean hatching rates of nest- fertilized eggs incubated in the round plastic buckets were 33.6%-76.3%, and in the net cages, were 5.9%-25.2%. Results showed that round bucket incubation was the best way for hatching fertilized eggs of mudskipper. Keeping the hatching seawater flowing is an important way for increasing the hatching rates of the mudskipper fertilized eggs.展开更多
[Objective] Aim to know the whole process of embryonic development of loach. [Method] DOM + LHRH-A2 was used to induce spawning of loach,then after fertilization,the embryos were cultured into freshwater water with te...[Objective] Aim to know the whole process of embryonic development of loach. [Method] DOM + LHRH-A2 was used to induce spawning of loach,then after fertilization,the embryos were cultured into freshwater water with temperature from 24 to 26 ℃ and pH from 7.0 to 7.5. The embryonic development of loach was observed and 27 concrete morphological characteristics and development time of loach from fertilized egg to newly hatched larval period were described in detail. [Result] The embryonic development of loach could be divided into cleavage stage,blastocyst stage,gastrula stage,neurula stage and organogenesis stage. The loach embryo from fertilized egg to out membrane period was 30 h 45 min in fresh water from 24 to 26 ℃ and pH from 7.0 to 7.5. [Conclusion] It provided important reference for studying artificial propagation and genetic breeding of loach.展开更多
Study on coastal processes in Kapar waters was conducted from 2008-2016.The aim of this study is to identify potential blood cockle(Anadara granosa)induce spawning ground based on physical intermittency.A total of 132...Study on coastal processes in Kapar waters was conducted from 2008-2016.The aim of this study is to identify potential blood cockle(Anadara granosa)induce spawning ground based on physical intermittency.A total of 132 sampling stations were recorded.A thermal power station situated 2 km away is discharging treated warm water(<40℃)on to the open sea causing the Sea Surface Temperature at near-by cockle culture to intermittent between 31-34℃ while the temperature at the bottom(at 6-8 m Mean Sea Level,MSL)is constant between 29.5℃ and 30.5℃.The results suggested that Kapar waters have two seasons of low waters annually(January-March and June-September,respectively)and the Lowest Astronomical Tide(LAT)occur in late January-February while Great Diurnal Tide is observed on the same period.The bathymetry suggested the present of deep water(6-8 m,MSL)towards the end of cockle culture lot which is closed to merchant shipping lane near Port Klang.Based on water elevation,an area of 255.4 ha within cockle culture lots were identified to have this bottom temperature intermittency(29.5-34.0℃)during its GT and it associated with cockle induce spawning condition.But this area was left out for cockle culture due to its typical depth(>3 m MSL)which prevents cockle farmers from harvesting due to their maximum of 3 m long hand dredge.Thus,this information could be useful for cockle sustainable management plan in near future.展开更多
基金Supported by the NSFC (No. 30170739) and also supported by the Provincial Natural Science Foundation of Fujian Province (No. B0210003).
文摘In this study, nest spawning was successfully induced by exogenous hormone injections and seawater flow stimulation, and optimum condition for hatching fertilized eggs of burrow fish mudskipper, Boleophthalmus pectinirostris, was searched. Apart from spawning inside the nests, females also spawned outside the nests. The percentages of spawned nests were 8.0% to 24.2%. Most eggs were observed adhered to the inner wall of the top half of the nest. Fertilization rates of the nest-spawned eggs varied from 17.3% to 80.8%. Females could spawn after being artificially confined inside the nests with males at ratios of 1:1 or 1:2, but the spawned eggs were not fertilized. Mean hatching rates of artificially fertilized eggs incubated in round plastic buckets were 32.7%-70.6%, and in the net cages, were 4.2%-20.5%, respectively. Mean hatching rates of nest- fertilized eggs incubated in the round plastic buckets were 33.6%-76.3%, and in the net cages, were 5.9%-25.2%. Results showed that round bucket incubation was the best way for hatching fertilized eggs of mudskipper. Keeping the hatching seawater flowing is an important way for increasing the hatching rates of the mudskipper fertilized eggs.
文摘[Objective] Aim to know the whole process of embryonic development of loach. [Method] DOM + LHRH-A2 was used to induce spawning of loach,then after fertilization,the embryos were cultured into freshwater water with temperature from 24 to 26 ℃ and pH from 7.0 to 7.5. The embryonic development of loach was observed and 27 concrete morphological characteristics and development time of loach from fertilized egg to newly hatched larval period were described in detail. [Result] The embryonic development of loach could be divided into cleavage stage,blastocyst stage,gastrula stage,neurula stage and organogenesis stage. The loach embryo from fertilized egg to out membrane period was 30 h 45 min in fresh water from 24 to 26 ℃ and pH from 7.0 to 7.5. [Conclusion] It provided important reference for studying artificial propagation and genetic breeding of loach.
文摘Study on coastal processes in Kapar waters was conducted from 2008-2016.The aim of this study is to identify potential blood cockle(Anadara granosa)induce spawning ground based on physical intermittency.A total of 132 sampling stations were recorded.A thermal power station situated 2 km away is discharging treated warm water(<40℃)on to the open sea causing the Sea Surface Temperature at near-by cockle culture to intermittent between 31-34℃ while the temperature at the bottom(at 6-8 m Mean Sea Level,MSL)is constant between 29.5℃ and 30.5℃.The results suggested that Kapar waters have two seasons of low waters annually(January-March and June-September,respectively)and the Lowest Astronomical Tide(LAT)occur in late January-February while Great Diurnal Tide is observed on the same period.The bathymetry suggested the present of deep water(6-8 m,MSL)towards the end of cockle culture lot which is closed to merchant shipping lane near Port Klang.Based on water elevation,an area of 255.4 ha within cockle culture lots were identified to have this bottom temperature intermittency(29.5-34.0℃)during its GT and it associated with cockle induce spawning condition.But this area was left out for cockle culture due to its typical depth(>3 m MSL)which prevents cockle farmers from harvesting due to their maximum of 3 m long hand dredge.Thus,this information could be useful for cockle sustainable management plan in near future.
