目的:探讨重组牛碱性成纤维细胞生长因子(rb-bFGF)滴眼液与羟糖苷滴眼液对年龄相关性白内障患者术后泪膜稳定性及干眼症状的影响。方法:以我院2015-01/2016-10收治的年龄相关性白内障术后患者80例80眼为研究对象,采用随机数字表法将患...目的:探讨重组牛碱性成纤维细胞生长因子(rb-bFGF)滴眼液与羟糖苷滴眼液对年龄相关性白内障患者术后泪膜稳定性及干眼症状的影响。方法:以我院2015-01/2016-10收治的年龄相关性白内障术后患者80例80眼为研究对象,采用随机数字表法将患者均分为对照组及试验组各40例40眼,两组分别实施羟糖苷滴眼液及rb-bFGF滴眼液治疗,观察治疗后两组临床疗效、炎症相关因子[白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)]、泪液分泌试验(S I t)、泪膜破裂时间(BUT)、角膜荧光素钠染色(FL)、眼部干眼症状评分。结果:治疗后试验组总有效率90.0%,较对照组72.5%显著高,差异有统计学意义(χ~2=4.021,P<0.05);治疗前两组IL-6、TNF-α、S I t、BUT、FL评分、眼部干眼症状评分比较无明显差异(P>0.05);治疗后两组IL-6、TNF-α、S I t、FL评分、眼部干眼症状评分较治疗前明显降低,BUT明显升高(P<0.05),且治疗后试验组的各项指标变化较对照组明显,差异有统计学意义(P<0.05)。结论:在年龄相关性白内障术后患者治疗中,rb-bFGF滴眼液在调节患者炎症因子表达。展开更多
Strobilanthes cusia is a traditional Chinese medicinal herb containing indole alkaloids,key bioactive components responsible for its pharmacological effects.However,there has been limited research regarding S.cusia co...Strobilanthes cusia is a traditional Chinese medicinal herb containing indole alkaloids,key bioactive components responsible for its pharmacological effects.However,there has been limited research regarding S.cusia compositional impact on antimicrobial properties.This study centered around the hydrolysis and oxidative condensation reactions of indican,aiming to investigate the influence of its intermediate,indoxyl,on pathogenic bacteria growth and biofilm formation.A notable inhibitory effect of indoxyl on Staphylococcus aureus biofilm formation was found.High-performance liquid chromatography and transmission electron microscopy confirmed that indoxyl disrupted the membrane structure of biofilm cells,causing a disturbance in cellular redox balance.These findings suggest that the oxidative reaction of indoxyl within cells could be a prominent factor affecting cell viability.Simultaneously,a portion of indoxyl oxidized externally to form indigo,which did not affect intracellular activities but rather disrupted the biofilm structure.The concept of“compositional changes”en-riches the understanding of antimicrobial properties of S.cusia,offering novel approaches for effectively inhibiting pathogenic microorganisms.展开更多
目的:探讨白内障超声乳化联合人工晶状体植入术前,应用人工泪液对合并干眼的糖尿病患者术后泪膜功能恢复的影响。方法:选择行白内障超声乳化联合人工晶状体植入前有干眼症的糖尿病患者54例60眼,随机分成A组、B组,A组28例30眼于术前使用...目的:探讨白内障超声乳化联合人工晶状体植入术前,应用人工泪液对合并干眼的糖尿病患者术后泪膜功能恢复的影响。方法:选择行白内障超声乳化联合人工晶状体植入前有干眼症的糖尿病患者54例60眼,随机分成A组、B组,A组28例30眼于术前使用羟糖苷滴眼液,B组26例30眼术前未使用羟糖苷滴眼液,两组术后均使用妥布霉素地塞米松、普拉洛芬及羟糖苷滴眼液,分别于术前3d,术后1,7,30,90d行干眼症状评分、泪膜破裂时间(break up time,BUT)、泪液分泌试验(SchirmerⅠtest,SⅠt)、角膜荧光素染色(fluorescein stain test,FL)评分。结果:手术前3d两组干眼症状评分、BUT,SⅠt,FI评分检查结果相比,无显著性差异(P>0.05),术后1,7d,A组干眼症状评分均小于B组,有显著性差异(P<0.05)。术后1,7,30d,A组与B组相比,BUT明显延长(P<0.05),FL着色密集度明显减少(P<0.05),SⅠt浸湿滤纸长度明显增加,差异均有统计学意义(P<0.05)。术后90d,两组干眼症状评分、BUT,SⅠt,FL评分值相比,差异均无统计学意义(P>0.05)。结论:超声乳化联合人工晶状体植入术前应用人工泪液,对合并干眼的糖尿病患者术后的早期干眼症状能得到有效改善,并且泪膜功能得到有效恢复。展开更多
文摘目的:探讨重组牛碱性成纤维细胞生长因子(rb-bFGF)滴眼液与羟糖苷滴眼液对年龄相关性白内障患者术后泪膜稳定性及干眼症状的影响。方法:以我院2015-01/2016-10收治的年龄相关性白内障术后患者80例80眼为研究对象,采用随机数字表法将患者均分为对照组及试验组各40例40眼,两组分别实施羟糖苷滴眼液及rb-bFGF滴眼液治疗,观察治疗后两组临床疗效、炎症相关因子[白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)]、泪液分泌试验(S I t)、泪膜破裂时间(BUT)、角膜荧光素钠染色(FL)、眼部干眼症状评分。结果:治疗后试验组总有效率90.0%,较对照组72.5%显著高,差异有统计学意义(χ~2=4.021,P<0.05);治疗前两组IL-6、TNF-α、S I t、BUT、FL评分、眼部干眼症状评分比较无明显差异(P>0.05);治疗后两组IL-6、TNF-α、S I t、FL评分、眼部干眼症状评分较治疗前明显降低,BUT明显升高(P<0.05),且治疗后试验组的各项指标变化较对照组明显,差异有统计学意义(P<0.05)。结论:在年龄相关性白内障术后患者治疗中,rb-bFGF滴眼液在调节患者炎症因子表达。
基金the support of the National KeyR&D Pro-gram of China(2021YFE0192600)the National Natural Science Foundation of China(32071640,51878646,22131044).
文摘Strobilanthes cusia is a traditional Chinese medicinal herb containing indole alkaloids,key bioactive components responsible for its pharmacological effects.However,there has been limited research regarding S.cusia compositional impact on antimicrobial properties.This study centered around the hydrolysis and oxidative condensation reactions of indican,aiming to investigate the influence of its intermediate,indoxyl,on pathogenic bacteria growth and biofilm formation.A notable inhibitory effect of indoxyl on Staphylococcus aureus biofilm formation was found.High-performance liquid chromatography and transmission electron microscopy confirmed that indoxyl disrupted the membrane structure of biofilm cells,causing a disturbance in cellular redox balance.These findings suggest that the oxidative reaction of indoxyl within cells could be a prominent factor affecting cell viability.Simultaneously,a portion of indoxyl oxidized externally to form indigo,which did not affect intracellular activities but rather disrupted the biofilm structure.The concept of“compositional changes”en-riches the understanding of antimicrobial properties of S.cusia,offering novel approaches for effectively inhibiting pathogenic microorganisms.
文摘目的:探讨白内障超声乳化联合人工晶状体植入术前,应用人工泪液对合并干眼的糖尿病患者术后泪膜功能恢复的影响。方法:选择行白内障超声乳化联合人工晶状体植入前有干眼症的糖尿病患者54例60眼,随机分成A组、B组,A组28例30眼于术前使用羟糖苷滴眼液,B组26例30眼术前未使用羟糖苷滴眼液,两组术后均使用妥布霉素地塞米松、普拉洛芬及羟糖苷滴眼液,分别于术前3d,术后1,7,30,90d行干眼症状评分、泪膜破裂时间(break up time,BUT)、泪液分泌试验(SchirmerⅠtest,SⅠt)、角膜荧光素染色(fluorescein stain test,FL)评分。结果:手术前3d两组干眼症状评分、BUT,SⅠt,FI评分检查结果相比,无显著性差异(P>0.05),术后1,7d,A组干眼症状评分均小于B组,有显著性差异(P<0.05)。术后1,7,30d,A组与B组相比,BUT明显延长(P<0.05),FL着色密集度明显减少(P<0.05),SⅠt浸湿滤纸长度明显增加,差异均有统计学意义(P<0.05)。术后90d,两组干眼症状评分、BUT,SⅠt,FL评分值相比,差异均无统计学意义(P>0.05)。结论:超声乳化联合人工晶状体植入术前应用人工泪液,对合并干眼的糖尿病患者术后的早期干眼症状能得到有效改善,并且泪膜功能得到有效恢复。
