The use of two inhibitors of Mek1/2 and Gsk3β(2i)promotes the generation of mouse diploid and haploid embryonic stem cells(ESCs)from the inner cell mass of biparental and uniparental blastocysts,respectively.However,...The use of two inhibitors of Mek1/2 and Gsk3β(2i)promotes the generation of mouse diploid and haploid embryonic stem cells(ESCs)from the inner cell mass of biparental and uniparental blastocysts,respectively.However,a system enabling long-term maintenance of imprints in ESCs has proven challenging.Here,we report that the use of a two-step a2i(alternative two inhibitors of Src and Gsk3β,TSa2i)derivation/culture protocol results in the establishment of androgenetic haploid ESCs(AG-haESCs)with stable DNA methylation at paternal DMRs(differentially DNA methylated regions)up to passage 60 that can efficiently support generating mice upon oocyte injection.We also show coexistence of H3K9me3 marks and ZFP57 bindings with intact DMR methylations.Furthermore,we demonstrate that TSa2itreated AG-haESCs are a heterogeneous cell population regarding paternal DMR methylation.Strikingly,AGhaESCs with late passages display increased paternal-DMR methylations and improved developmental potential compared to early-passage cells,in part through the enhanced proliferation of H19-DMR hypermethylated cells.Together,we establish AG-haESCs that can longterm maintain paternal imprints.展开更多
Tellurene,a chiral chain semiconductor with a narrow bandgap and exceptional strain sensitivity,emerges as a pivotal material for tailoring electronic and optoelectronic properties via strain engineering.This study el...Tellurene,a chiral chain semiconductor with a narrow bandgap and exceptional strain sensitivity,emerges as a pivotal material for tailoring electronic and optoelectronic properties via strain engineering.This study elucidates the fundamental mechanisms of ultrafast laser shock imprinting(LSI)in two-dimensional tellurium(Te),establishing a direct relationship between strain field orientation,mold topology,and anisotropic structural evolution.This is the first demonstration of ultrafast LSI on chiral chain Te unveiling orientation-sensitive dislocation networks.By applying controlled strain fields parallel or transverse to Te’s helical chains,we uncover two distinct deformation regimes.Strain aligned parallel to the chain’s direction induces gliding and rotation governed by weak interchain interactions,preserving covalent intrachain bonds and vibrational modes.In contrast,transverse strain drives shear-mediated multimodal deformations—tensile stretching,compression,and bending—resulting in significant lattice distortions and electronic property modulation.We discovered the critical role of mold topology on deformation:sharp-edged gratings generate localized shear forces surpassing those from homogeneous strain fields via smooth CD molds,triggering dislocation tangle formation,lattice reorientation,and inhomogeneous plastic deformation.Asymmetrical strain configurations enable localized structural transformations while retaining single-crystal integrity in adjacent regions—a balance essential for functional device integration.These insights position LSI as a precision tool for nanoscale strain engineering,capable of sculpting 2D material morphologies without compromising crystallinity.By bridging ultrafast mechanics with chiral chain material science,this work advances the design of strain-tunable devices for next-generation electronics and optoelectronics,while establishing a universal framework for manipulating anisotropic 2D systems under extreme strain rates.This work discovered crystallographic orientation-dependent deformation mechanisms in 2D Te,linking parallel strain to chain gliding and transverse strain to shear-driven multimodal distortion.It demonstrates mold geometry as a critical lever for strain localization and dislocation dynamics,with sharp-edged gratings enabling unprecedented control over lattice reorientation.Crucially,the identification of strain field conditions that reconcile severe plastic deformation with single-crystal retention offers a pathway to functional nanostructure fabrication,redefining LSI’s potential in ultrafast strain engineering of chiral chain materials.展开更多
As a typical bioflavonoid,diosmetin is desirable in the field of natural medicine,healthy food,and cosmetics by anti-cancer,antibacterial,antioxidant,estrogen-like and anti-inflammatory activities,and it comes from a ...As a typical bioflavonoid,diosmetin is desirable in the field of natural medicine,healthy food,and cosmetics by anti-cancer,antibacterial,antioxidant,estrogen-like and anti-inflammatory activities,and it comes from a wide range of sources in traditional Chinese medicine like spider fragrance,spearmint and chrysanthemum,as well as in Citrus fruit.However,traditional analytical methods such as silica gel column chromatography face multiple challenges in the selective extraction of diosmetin from biological materials and traditional Chinese medicinal materials.Therefore,it is urgent to develop a new type of absorbent with high efficiency,recyclability and good specificity to diosmetin.In this investigation,a magnetic surface molecularly imprinted polymer(labeled as Diosmetin/SMIPs)was synthesized employing magnetic nanoparticles as the carrier and 4-vinylpyridinyl(4-VP)as the functional monomer by surface imprinting technology.The functional monomer was screened by the binding energy(△E)between functional monomers and template molecules via computational simulation.The Diosmetin/SMIPs had a high level of specific recognition and adsorption capability towards diosmetin with a 20.25 mg g^(-1) adsorption capacity and an imprinting factor(IF)of 2.28.Additionally,it demonstrated excellent regeneration performance with 8 adsorption/desorption cycles.In addition,91.20%-94.16% of spiked diosmetin was recovered from the lemon peel samples.The strategy of constructing Diosmetin/SMIPs based on computational simulation can effectively enhance the specific adsorption performance of diosmetin.Meanwhile,Diosmetin/SMIPs synthesized by imprinting polymerization showed excellent anti-interference and reusability,and realized efficient targeted extraction of diosmetin from lemon peel samples.The results of this investigation provide a promising adsorbent for selective enrichment of diosmetin from Citrus fruit and complicated materials.展开更多
In this work,by combining the specific selectivity of molecularly imprinted polymer(MIP)with a simple and sensitive electrochemiluminescence(ECL)detection method,a molecularly imprinted ECL sensor for kanamycin(KA)was...In this work,by combining the specific selectivity of molecularly imprinted polymer(MIP)with a simple and sensitive electrochemiluminescence(ECL)detection method,a molecularly imprinted ECL sensor for kanamycin(KA)was developed.The MIP film was synthesized on the surface of gold electrode via electrochemical polymerization,using pyrrole(PY)as the functional monomer and KA as the template molecule.The commonly used luminescent reagent Ru(bpy)32+was employed as the ECL probe,and its co-reagent tripropylamine(TPA)was added to enhance the detection sensitivity of the sensor.Quantitative analysis of KA was performed by evaluating the difference in ECL responses between MIP film electrodes after KA removal and KA rebinding.The sensor exhibited high selectivity,good reproducibility,and stability toward KA,with a linear range of 5.00×10^(-8) to 1.00×10^(-5) mol·L^(-1) and a limit of detection of 1.67×10^(-8) mol·L^(-1)(S/N=3).This work would provide a new idea for the development of simple and sensitive molecularly imprinted ECL sensors.展开更多
Molecularly imprinted polymers(MIPs)are a kind of synthetic receptors possessing wide application prospects in proteins recognition.However,there are still great challenges in proteins imprinting due to their large si...Molecularly imprinted polymers(MIPs)are a kind of synthetic receptors possessing wide application prospects in proteins recognition.However,there are still great challenges in proteins imprinting due to their large size and easy conformation change.In this study,we explored epitope-oriented MIP based on host-vip interaction(hg-MIP)and constructed a novel hg-MIP-SERS(surface-enhanced Raman scatting)approach for efficiently recognizing the terminal epitopes of neuron-specific enolase(NSE),a well-known disease biomarker for small cell lung cancer,neuroblstom,and Alzheimer's disease.The C-and N-terminal epitopes of NSE were modified with 4-(phenylazo)benzoic acid,then they were used as the templates and immobilized onβ-cyclodextrin-functionalized substrates.The imprinted layer was formed by polymerization of various functional monomers.Combined with SERS detection,an antibody-free sandwich assay based on hg-MIP was successfully used to detect the concentration of NSE in human serums,with the advantages of simple operation,small sample volume(5μL),wide linear range(1–10^(4)ng/m L)and a limit of detection as low as 0.01 ng/m L.The developed epitope-oriented hg-MIP-SERS approach can also be extended to other proteins,expanding the imprinting method of proteins,and has a broad development space in the field of protein separation and detection.展开更多
Bromocresol green(BCG)and malachite green(MG)are water-soluble toxic organic dyes with adverse health and environmental implications.This study presented a conjugate imprinted adsorbent(CIA)synthesized by incorporatin...Bromocresol green(BCG)and malachite green(MG)are water-soluble toxic organic dyes with adverse health and environmental implications.This study presented a conjugate imprinted adsorbent(CIA)synthesized by incorporating trimethoprim vanillin ligand into a highly crosslinked polymer,designed for the efficient removal of BCG and MG from wastewater.Characterization of CIA involved X-ray powder diffraction,Fourier transform infrared,and scanning electron microscopic analyses.Batch adsorption processes were conducted to evaluate the adsorption characteristics of CIA,with focuses on the effects of contact time,initial dye concentration,pH,and temperature.The molecularly imprinted polymers(MIPs)achieved removal efficiencies of 99.27%and 98.99%at equilibrium for BCG and MG adsorption,respectively.The non-imprinted polymers(NIPs)demonstrated BCG and MG adsorption efficiencies of 51.52%and 62.90%at equilibrium,respectively.Kinetic and isotherm models were employed to elucidate the BCG and MG adsorption mechanisms.The thermodynamic results indicated non-spontaneous and spontaneous reactions for BCG and MG adsorption on MIPs under the examined temperature conditions.The adsorbent exhibited sustained high removal efficiency through five reuse cycles,with no apparent reduction in adsorption performance.Validation of the adsorbent using real textile wastewater samples achieved BCG and MG removal efficiencies of 85.5%-87.5%.The adsorbent outperformed previously reported materials in BCG and MG adsorption.The synthesized CIA is a promising adsorbent for BCG and MG dye removal,contributing to water sustainability.展开更多
Phase imprinting enables the dynamic generation of superflow in bosonic atoms,effectively overcoming traditional limitations such as vortex number constraints and heating effects.However,the mechanisms underlying supe...Phase imprinting enables the dynamic generation of superflow in bosonic atoms,effectively overcoming traditional limitations such as vortex number constraints and heating effects.However,the mechanisms underlying superflow formation remain insufficiently understood.In this work,we reveal these mechanisms by studying the time evolution of the transferred total angular momentum and the quantized current throughout the phase imprinting process,achieved through numerically solving the time-dependent Schrodinger and Gross-Pitaevskii equations.We demonstrate that the Bose gas dynamically acquires angular momentum through the density depletion induced by the phase imprinting potential,whereas quantized currents emerge from azimuthal phase slips accompanied by complete density depletions.Regarding the impact of system parameters,such as interactions,we find that interactions hinder superflow formation,as the azimuthal density distribution becomes less susceptible to the phase imprinting potential.Our findings offer microscopic insights into the dynamic development of superflow during the phase imprinting process and provide valuable guidance for ongoing experimental efforts.展开更多
This work develops a protein imprinted nanosphere with varied recognition specificity for bovine serum albumin(BSA)and lysozyme(Lyz)under different UV light through a gradient dual crosslinked imprinting strategy(i.e....This work develops a protein imprinted nanosphere with varied recognition specificity for bovine serum albumin(BSA)and lysozyme(Lyz)under different UV light through a gradient dual crosslinked imprinting strategy(i.e.,covalent crosslinking and dynamic reversible crosslinking).The imprinting cavities are initially constructed using irreversible covalent crosslinking to specifically recognize BSA,and then the coumarin residues in the imprinting cavities are crosslinked under 365 nm UV light to further imprint Lyz,because Lyz has smaller size than BSA.Since the photo-crosslinking of coumarin is a reversible reaction,the imprinting cavities of Lyz can be de-crosslinked under 254 nm UV light and restore the imprinting cavities of BSA.Moreover,the N-isopropyl acrylamide(NIPAM)and pyrrolidine residues copolymerized in the polymeric surface of the nanospheres are temperature-and p H-responsive respectively.Therefore,the protein rebinding and release behaviors of the nanospheres are controlled by external temperature and p H.As a result,the materials can selectively separate BSA from real bovine whole blood and Lyz from egg white under different UV light.This study may provide a new strategy for construction of protein imprinted materials with tunable specificity for different proteins.展开更多
The reduced elastic modulus Er and indentation hardness HIT of various brittle solids including ceramics,semiconductors,glasses,single crystals,and laser material were evaluated using nanoindentation.Various analysis ...The reduced elastic modulus Er and indentation hardness HIT of various brittle solids including ceramics,semiconductors,glasses,single crystals,and laser material were evaluated using nanoindentation.Various analysis procedures were compared such as Oliver&Pharr and nominal hardness-based methods,which require area function of the indenter,and other methods based on energy,displacement,contact depth,and contact stiffness,which do not require calibration of the indenter.Elastic recovery of the imprint by the Knoop indenter was also utilized to evaluate elastic moduli of brittle solids.Expressions relating HIT/Er and dimensionless nanoindentation variables(e.g.,the ratio of elastic work over total work and the ratio of permanent displacement over maximum displacement)are found to be nonlinear rather than linear for brittle solids.The plastic hardness Hp of brittle solids(except traditional glasses)extracted based on Er is found to be proportional to E_(r)√H_(IT).展开更多
In this work,a novel electrochemical sensor based on covalent organic framework@carbon black@molecularly imprinted polydopamine(COF@CB@MPDA)was developed for selective recognition and determination of ciprofloxacin(CF...In this work,a novel electrochemical sensor based on covalent organic framework@carbon black@molecularly imprinted polydopamine(COF@CB@MPDA)was developed for selective recognition and determination of ciprofloxacin(CF).COF@CB@MPDA possessed good water dispersibility and was synthesized by the selfpolymerization of dopamine under alkaline conditions in the presence of the COF,CB and CF.The high surface area COF enhanced the adsorption of CF,whilst CB gave the composites high electrical conductivity to improve the sensitivity of the proposed COF@CB@MPDA/glassy carbon electrode(GCE)sensor.The specific recognition of CF by COF@CB@MPDA involved hydrogen bonding and van der Waals interactions.Under optimized conditions,the sensor showed a good linear relationship with CF concentration over the range of 5.0×10^(–7)and 1.0×10^(–4)mol/L,with a limit of detection(LOD)of 9.53×10^(–8)mol/L.Further,the developed sensor exhibited high selectivity,repeatability and stability for CF detection in milk and milk powders.The method used to fabricate the COF@CB@MPDA/GCE sensor could be easily adapted for the selective recognition and detection of other antibacterial agents and organic pollutants in the environment.展开更多
Gestational diabetes mellitus(GDM)is increasingly recognized not only for its immediate obstetric complications but also for its long-term metabolic consequences in both mothers and their offspring.Traditionally,resea...Gestational diabetes mellitus(GDM)is increasingly recognized not only for its immediate obstetric complications but also for its long-term metabolic consequences in both mothers and their offspring.Traditionally,research has emphasized the roles of pancreaticβ-cell dysfunction and placental dysregulation in GDM.However,emerging evidence highlights the maternal liver as a central metabolic hub during pregnancy coordinating glucose,lipid,and hormonal adaptations essential for fetal development.This review synthesizes current findings on how GDM disrupts the maternal liver’s adaptive roles,transforming it from a metabolic coordinator into a source of maladaptive endocrine,inflammatory,and nutrient signals.It outlines key mechanistic pathways through which maternal hepatic dysfunction may increase offspring susceptibility to non-alcoholic fatty liver disease and type 2 diabetes mellitus.These include hepatokine dysregulation,altered lipid metabolism,impaired insulin signaling,inflammatory and oxidative stress pathways,and epigenetic and transcriptomic reprogramming.In addition,it explores novel axes such as the gut-liver-placenta interplay,bile acid signaling disruptions,endoplasmic reticulum stress responses,and extracellular vesiclemediated communication.By reframing the maternal liver’s role in GDM pathophysiology,this review identifies critical windows for early clinical intervention and highlights the potential for liver-focused strategies to prevent the intergenerational transmission of metabolic disease.展开更多
Maize(Zea mays L.)is a globally significant crop essential for food,feed,and bioenergy production.The maize kernel,serving as a primary sink for starch,proteins,lipids,and essential micronutrients,is crucial for enhan...Maize(Zea mays L.)is a globally significant crop essential for food,feed,and bioenergy production.The maize kernel,serving as a primary sink for starch,proteins,lipids,and essential micronutrients,is crucial for enhancing maize yield and quality.Previous studies have established the critical role of Polycomb Repressive Complex 2(PRC2)in regulating kernel development.In this study,we applied a reverse genetics approach to investigate the role of ZmFIE1,the homolog of the PRC2 complex component Extra sex combs(Esc),in maize development.The functional loss of ZmFIE1 significantly reduces embryo size in the early stage but has a relatively small impact on mature kernels.Integrating transcriptional and metabolomic profiling suggests that ZmFIE1 is involved in regulating nutrient balance between the endosperm and embryo.In addition,we demonstrate that ZmFIE1 is maternally expressed,and that the maternal inheritance of the fie1 allele significantly affects the imprinting status of paternally imprinted genes.Overall,our results suggest that ZmFIE1 is a key gene involved in the modulation of embryo development via regulating genomic imprinting and nutrient balance between embryo and endosperm,which provides new insights into the regulation mechanism underlying kernel development.展开更多
BACKGROUND Silver-Russell syndrome(SRS)is a clinically heterogeneous entity characterized by intrauterine and postnatal growth restriction,relative macrocephaly at birth,distinct facial features,and body asymmetry com...BACKGROUND Silver-Russell syndrome(SRS)is a clinically heterogeneous entity characterized by intrauterine and postnatal growth restriction,relative macrocephaly at birth,distinct facial features,and body asymmetry combined with other malformations.CASE SUMMARY Herein,we describe four individuals with SRS,focusing on their prenatal phenotype,postnatal presentation,diagnosis,and management.All cases had a typical phenotype,including postnatal growth failure,short stature(chronic malnutrition),and protruding forehead.Individually,they presented with feeding difficulties,leg length discrepancy,triangular face,or relative macrocephaly at birth,and each one exhibited distinct SRS features,including motor and/or speech delay,experiencing frequent hypoglycemic episodes.The fact that each patient exhibited a different combination of clinical findings underlines the heterogeneity of the syndrome.CONCLUSION SRS is diagnosed clinically.However,only 60%of cases are genetically confirmed,while most are sporadic.Although SRS is a well-described syndrome,a delayed diagnosis can have grave consequences on a child’s growth.Recombinant human growth hormone treatment is often initiated shortly after the diagnosis.The follow-up requires a multidisciplinary approach.展开更多
Quantitative determination of tetracycline(TC)in environment and foods is of great importance,as excessive residues might have negative effects on human health and environmental risks.Herein,a selfpowered molecularly ...Quantitative determination of tetracycline(TC)in environment and foods is of great importance,as excessive residues might have negative effects on human health and environmental risks.Herein,a selfpowered molecularly imprinted photoelectrochemical(PEC)sensor based on the Zn O/C photoanode and the Fe-doped CuBi_(2)O_(4)(CBFO)photocathode is developed for the sensitive detection of TC.The photocathodic current can be amplified by the efficient electron transfer caused by the Fermi energy level gap between the photoanode and photocathode.Furthermore,molecularly imprinted polymers(MIPs)at photocathode can selectivity identify the TC templates and thus improve the specificity.Under the optimal conditions,the sensor has a linear range of 10^(-2)-1.0×10^(5) nmol/L,and a limit of detection(LOD)of 0.007 nmol/L(S/N=3).More crucially,the milk sample detection is carried out using the as-prepared sensor,and the outcome is satisfactory.The research gives us a novel sensing platform for quick and accurate antibiotic(like TC)in environment and food monitoring.展开更多
Methacrylic acid was used together with a molecular imprinting technique to modify chitosan. In addition, the adsorption kinetics and adsorption isotherms were recorded and the results were analyzed to investigate rep...Methacrylic acid was used together with a molecular imprinting technique to modify chitosan. In addition, the adsorption kinetics and adsorption isotherms were recorded and the results were analyzed to investigate reparative adsorption for Cr（VI） from the polluted Xiangjiang River water. A comparative X-ray analysis shows that the degree of crystallization in the imprinted polymer was significantly weakened, the area of the non-crystalline region was larger. There were more adsorption sites in the imprinted polymer, and the adsorption capacity towards Cr（VI） was increased. The adsorption capacity of the imprinted polymer towards Cr（VI） increased with time and reaches saturation after 8 h. The optimal adsorption time was 4-8 h after the adsorption starting and the optimal pH value for the solution was in the range of 4.5-7.5. When the chitosan reaches saturation, the adsorption capacity achieves a state of equilibrium, and the maximum Cr（VI） extraction rate reaches 33.7%. Moreover, the adsorption capacity of the imprinted polymer towards Cr（VI） increases with increasing chitosan concentration. In this situation, the Cr（VI） extraction rate shows little variation, and the maximum removal rate can reach 98.3%. Furthermore, the Cr（VI） extraction rate increases with an increase in the degree of deacetylation in the chatoyant and chitosan, with the best adsorption effect corresponding to 90% deacetylation. Fitting the adsorption data to the quasi first- and second-order kinetic models yields correlation coefficients of 0.9013 and 0.9875, respectively. The corresponding rate constants for the two models are 0.0091 min-1 and 7.129 g/（mg.min）, respectively. Hence, the adsorption using Cr（VI）-imprinted chitosan is more consistent with the second-order kinetics. Comparing the data to Freundlich and Langrnuir adsorption isotherms shows that the latter has a better linear fit and a maximum adsorption capacity of 15.784 mg/g.展开更多
Solid phase extraction （SPE） is a widely used sample pretreatment method for separation, purification and enrichment, which has been established due to its significant advantages of time-saving, low consumption of s...Solid phase extraction （SPE） is a widely used sample pretreatment method for separation, purification and enrichment, which has been established due to its significant advantages of time-saving, low consumption of solvent, high enrichment factor, high accuracy, etc. In recent years, a variety of new SPE methods such as molecularly imprinted solid phase extraction （MISPE）, magnetic solid phase extraction （MSPE）, solid phase micro-extraction （SPME）, etc., which are superior to the conventional SPE, have been developed and been widely applied to food, drugs, and environmental monitoring. In this paper, the basic principles and methods of SPE and its new applications in different areas are reviewed.展开更多
Prader-Willi Syndrome （PWS） is a genetic disorder that is difficult to detect, particularly at an early age. PWS is caused by disruption of normal, epigenetically controlled gene function in the chromosome 15q11-q13...Prader-Willi Syndrome （PWS） is a genetic disorder that is difficult to detect, particularly at an early age. PWS is caused by disruption of normal, epigenetically controlled gene function in the chromosome 15q11-q13 region. Clinical symptoms are difficult to diagnose in infants and only become clearer at later ages as the patients develop hyperphagia and morbid obesity. Molecular genetic tests are able to definitively diagnose PWS and allow early diagnosis of the syndrome. High resolution cytogenetic testing, methylation-specific PCR （MS-PCR）, and linkage analysis are routinely used to diagnose PWS. To establish a linkage analysis method for Chinese patients, this study identified a useful set of STR markers in the typical PWS deletion and adjacent area, for linkage analysis in two Chinese families with PWS offspring. Using this method, the authors confn＇rned that one patient had a paternal deletion in chromosome 15q 11-q 13 and the other patient had maternal uniparental heterodisomy of chromosome 15. MS -PCR and high resolution chromosome G-banding also confirmed this diagnosis. This linkage analysis method can detect both deletion and uniparental disomy, thus providing valuable information for genetic counseling and the opportunity to analyze the relationship between the genotype and phenotype of PWS.展开更多
Genomic imprinting is the epigenetic phenomenon by which certain genes are expressed in a parent-of-origin-specific manner, and was first discovered in mammalian embryos. Recent studies have shown that it also occurs ...Genomic imprinting is the epigenetic phenomenon by which certain genes are expressed in a parent-of-origin-specific manner, and was first discovered in mammalian embryos. Recent studies have shown that it also occurs in developing plant seeds, and is now becoming a hot topic of biology of plant seed development. According to the previous studies on imprinted genes, imprinting mechanism and their roles in plant seed development, the current progress of genomic imprinting in plant seed development was summarized and possible strategies were proposed to deal with the problems, which could provide helpful information for further research.展开更多
基金This study was supported by Genome Tagging Project and grants from the Chinese Academy of Sciences,the National Key Research and Development Program of Chinathe National Natural Science Foundation of China(2019YFA0109900,2020YFA0509000,XDB19010204,QYZDJ-SSW-SMC023,Facility-based Open Research Program,31821004,32030029,and 31730062).
文摘The use of two inhibitors of Mek1/2 and Gsk3β(2i)promotes the generation of mouse diploid and haploid embryonic stem cells(ESCs)from the inner cell mass of biparental and uniparental blastocysts,respectively.However,a system enabling long-term maintenance of imprints in ESCs has proven challenging.Here,we report that the use of a two-step a2i(alternative two inhibitors of Src and Gsk3β,TSa2i)derivation/culture protocol results in the establishment of androgenetic haploid ESCs(AG-haESCs)with stable DNA methylation at paternal DMRs(differentially DNA methylated regions)up to passage 60 that can efficiently support generating mice upon oocyte injection.We also show coexistence of H3K9me3 marks and ZFP57 bindings with intact DMR methylations.Furthermore,we demonstrate that TSa2itreated AG-haESCs are a heterogeneous cell population regarding paternal DMR methylation.Strikingly,AGhaESCs with late passages display increased paternal-DMR methylations and improved developmental potential compared to early-passage cells,in part through the enhanced proliferation of H19-DMR hypermethylated cells.Together,we establish AG-haESCs that can longterm maintain paternal imprints.
基金financial support from NSF ExpandQISE program.The synthesis of tellurene was supported by NSF under grant no.CMMI-2046936supports from Purdue Research Foundation.
文摘Tellurene,a chiral chain semiconductor with a narrow bandgap and exceptional strain sensitivity,emerges as a pivotal material for tailoring electronic and optoelectronic properties via strain engineering.This study elucidates the fundamental mechanisms of ultrafast laser shock imprinting(LSI)in two-dimensional tellurium(Te),establishing a direct relationship between strain field orientation,mold topology,and anisotropic structural evolution.This is the first demonstration of ultrafast LSI on chiral chain Te unveiling orientation-sensitive dislocation networks.By applying controlled strain fields parallel or transverse to Te’s helical chains,we uncover two distinct deformation regimes.Strain aligned parallel to the chain’s direction induces gliding and rotation governed by weak interchain interactions,preserving covalent intrachain bonds and vibrational modes.In contrast,transverse strain drives shear-mediated multimodal deformations—tensile stretching,compression,and bending—resulting in significant lattice distortions and electronic property modulation.We discovered the critical role of mold topology on deformation:sharp-edged gratings generate localized shear forces surpassing those from homogeneous strain fields via smooth CD molds,triggering dislocation tangle formation,lattice reorientation,and inhomogeneous plastic deformation.Asymmetrical strain configurations enable localized structural transformations while retaining single-crystal integrity in adjacent regions—a balance essential for functional device integration.These insights position LSI as a precision tool for nanoscale strain engineering,capable of sculpting 2D material morphologies without compromising crystallinity.By bridging ultrafast mechanics with chiral chain material science,this work advances the design of strain-tunable devices for next-generation electronics and optoelectronics,while establishing a universal framework for manipulating anisotropic 2D systems under extreme strain rates.This work discovered crystallographic orientation-dependent deformation mechanisms in 2D Te,linking parallel strain to chain gliding and transverse strain to shear-driven multimodal distortion.It demonstrates mold geometry as a critical lever for strain localization and dislocation dynamics,with sharp-edged gratings enabling unprecedented control over lattice reorientation.Crucially,the identification of strain field conditions that reconcile severe plastic deformation with single-crystal retention offers a pathway to functional nanostructure fabrication,redefining LSI’s potential in ultrafast strain engineering of chiral chain materials.
基金supported by the National Natural Science Foundation of China(Nos.32301259,32101228,32271527 and 32371536)the“Pioneer”and“Leading Goose”R&D Program of Zhejiang(Nos.2022C02023 and 2023C02015)+1 种基金the Research Foundation of Talented Scholars of Zhejiang A&F University(No.2021LFR058)the Dean-ship of Scientific Research at Northern Border University,Arar,KSA for funding this research work through the project number“NBU-FPEJ-2024-177-01”.
文摘As a typical bioflavonoid,diosmetin is desirable in the field of natural medicine,healthy food,and cosmetics by anti-cancer,antibacterial,antioxidant,estrogen-like and anti-inflammatory activities,and it comes from a wide range of sources in traditional Chinese medicine like spider fragrance,spearmint and chrysanthemum,as well as in Citrus fruit.However,traditional analytical methods such as silica gel column chromatography face multiple challenges in the selective extraction of diosmetin from biological materials and traditional Chinese medicinal materials.Therefore,it is urgent to develop a new type of absorbent with high efficiency,recyclability and good specificity to diosmetin.In this investigation,a magnetic surface molecularly imprinted polymer(labeled as Diosmetin/SMIPs)was synthesized employing magnetic nanoparticles as the carrier and 4-vinylpyridinyl(4-VP)as the functional monomer by surface imprinting technology.The functional monomer was screened by the binding energy(△E)between functional monomers and template molecules via computational simulation.The Diosmetin/SMIPs had a high level of specific recognition and adsorption capability towards diosmetin with a 20.25 mg g^(-1) adsorption capacity and an imprinting factor(IF)of 2.28.Additionally,it demonstrated excellent regeneration performance with 8 adsorption/desorption cycles.In addition,91.20%-94.16% of spiked diosmetin was recovered from the lemon peel samples.The strategy of constructing Diosmetin/SMIPs based on computational simulation can effectively enhance the specific adsorption performance of diosmetin.Meanwhile,Diosmetin/SMIPs synthesized by imprinting polymerization showed excellent anti-interference and reusability,and realized efficient targeted extraction of diosmetin from lemon peel samples.The results of this investigation provide a promising adsorbent for selective enrichment of diosmetin from Citrus fruit and complicated materials.
文摘In this work,by combining the specific selectivity of molecularly imprinted polymer(MIP)with a simple and sensitive electrochemiluminescence(ECL)detection method,a molecularly imprinted ECL sensor for kanamycin(KA)was developed.The MIP film was synthesized on the surface of gold electrode via electrochemical polymerization,using pyrrole(PY)as the functional monomer and KA as the template molecule.The commonly used luminescent reagent Ru(bpy)32+was employed as the ECL probe,and its co-reagent tripropylamine(TPA)was added to enhance the detection sensitivity of the sensor.Quantitative analysis of KA was performed by evaluating the difference in ECL responses between MIP film electrodes after KA removal and KA rebinding.The sensor exhibited high selectivity,good reproducibility,and stability toward KA,with a linear range of 5.00×10^(-8) to 1.00×10^(-5) mol·L^(-1) and a limit of detection of 1.67×10^(-8) mol·L^(-1)(S/N=3).This work would provide a new idea for the development of simple and sensitive molecularly imprinted ECL sensors.
基金supported by Open Project of State Key Laboratory of Supramolecular Structure and Materials,Jilin University,China(No.sklssm2024018)。
文摘Molecularly imprinted polymers(MIPs)are a kind of synthetic receptors possessing wide application prospects in proteins recognition.However,there are still great challenges in proteins imprinting due to their large size and easy conformation change.In this study,we explored epitope-oriented MIP based on host-vip interaction(hg-MIP)and constructed a novel hg-MIP-SERS(surface-enhanced Raman scatting)approach for efficiently recognizing the terminal epitopes of neuron-specific enolase(NSE),a well-known disease biomarker for small cell lung cancer,neuroblstom,and Alzheimer's disease.The C-and N-terminal epitopes of NSE were modified with 4-(phenylazo)benzoic acid,then they were used as the templates and immobilized onβ-cyclodextrin-functionalized substrates.The imprinted layer was formed by polymerization of various functional monomers.Combined with SERS detection,an antibody-free sandwich assay based on hg-MIP was successfully used to detect the concentration of NSE in human serums,with the advantages of simple operation,small sample volume(5μL),wide linear range(1–10^(4)ng/m L)and a limit of detection as low as 0.01 ng/m L.The developed epitope-oriented hg-MIP-SERS approach can also be extended to other proteins,expanding the imprinting method of proteins,and has a broad development space in the field of protein separation and detection.
文摘Bromocresol green(BCG)and malachite green(MG)are water-soluble toxic organic dyes with adverse health and environmental implications.This study presented a conjugate imprinted adsorbent(CIA)synthesized by incorporating trimethoprim vanillin ligand into a highly crosslinked polymer,designed for the efficient removal of BCG and MG from wastewater.Characterization of CIA involved X-ray powder diffraction,Fourier transform infrared,and scanning electron microscopic analyses.Batch adsorption processes were conducted to evaluate the adsorption characteristics of CIA,with focuses on the effects of contact time,initial dye concentration,pH,and temperature.The molecularly imprinted polymers(MIPs)achieved removal efficiencies of 99.27%and 98.99%at equilibrium for BCG and MG adsorption,respectively.The non-imprinted polymers(NIPs)demonstrated BCG and MG adsorption efficiencies of 51.52%and 62.90%at equilibrium,respectively.Kinetic and isotherm models were employed to elucidate the BCG and MG adsorption mechanisms.The thermodynamic results indicated non-spontaneous and spontaneous reactions for BCG and MG adsorption on MIPs under the examined temperature conditions.The adsorbent exhibited sustained high removal efficiency through five reuse cycles,with no apparent reduction in adsorption performance.Validation of the adsorbent using real textile wastewater samples achieved BCG and MG removal efficiencies of 85.5%-87.5%.The adsorbent outperformed previously reported materials in BCG and MG adsorption.The synthesized CIA is a promising adsorbent for BCG and MG dye removal,contributing to water sustainability.
基金supported by the National Natural Science Foundation of China(Grants Nos.12104406 and 12204105)the Natural Science Foundation Zhejiang Province,China(Grant No.ZCLMS25A0401)+1 种基金the Startup Grant of Zhejiang SciTech University(Grant No.21062338-Y)the Natural Science Foundation of Fujian Province,China(Grant No.2022J05116)。
文摘Phase imprinting enables the dynamic generation of superflow in bosonic atoms,effectively overcoming traditional limitations such as vortex number constraints and heating effects.However,the mechanisms underlying superflow formation remain insufficiently understood.In this work,we reveal these mechanisms by studying the time evolution of the transferred total angular momentum and the quantized current throughout the phase imprinting process,achieved through numerically solving the time-dependent Schrodinger and Gross-Pitaevskii equations.We demonstrate that the Bose gas dynamically acquires angular momentum through the density depletion induced by the phase imprinting potential,whereas quantized currents emerge from azimuthal phase slips accompanied by complete density depletions.Regarding the impact of system parameters,such as interactions,we find that interactions hinder superflow formation,as the azimuthal density distribution becomes less susceptible to the phase imprinting potential.Our findings offer microscopic insights into the dynamic development of superflow during the phase imprinting process and provide valuable guidance for ongoing experimental efforts.
基金financial support from the National Natural Science Foundation of China(No.22275148)National Key R&D Program of China(No.2018YFB1900201)for Qiuyu Zhang+2 种基金the National Natural Science Foundation of China(No.22271232)Fundamental Research Funds for the Central Universities(No.D5000230114)for Shixin Fathe Fundamental Research Funds for the Central Universities(No.D5000220339)for Qing Liu。
文摘This work develops a protein imprinted nanosphere with varied recognition specificity for bovine serum albumin(BSA)and lysozyme(Lyz)under different UV light through a gradient dual crosslinked imprinting strategy(i.e.,covalent crosslinking and dynamic reversible crosslinking).The imprinting cavities are initially constructed using irreversible covalent crosslinking to specifically recognize BSA,and then the coumarin residues in the imprinting cavities are crosslinked under 365 nm UV light to further imprint Lyz,because Lyz has smaller size than BSA.Since the photo-crosslinking of coumarin is a reversible reaction,the imprinting cavities of Lyz can be de-crosslinked under 254 nm UV light and restore the imprinting cavities of BSA.Moreover,the N-isopropyl acrylamide(NIPAM)and pyrrolidine residues copolymerized in the polymeric surface of the nanospheres are temperature-and p H-responsive respectively.Therefore,the protein rebinding and release behaviors of the nanospheres are controlled by external temperature and p H.As a result,the materials can selectively separate BSA from real bovine whole blood and Lyz from egg white under different UV light.This study may provide a new strategy for construction of protein imprinted materials with tunable specificity for different proteins.
基金supported by the National Natural Science Foundation of China (Grant No.51705082)Fujian Provincial Minjiang Scholar Program (Grant No.0020-510759)+1 种基金Qishan Sholar program in Fuzhou University (Grant No.0020-650289)Fuzhou University Testing Fund of precious apparatus (Grant No.2023T018).
文摘The reduced elastic modulus Er and indentation hardness HIT of various brittle solids including ceramics,semiconductors,glasses,single crystals,and laser material were evaluated using nanoindentation.Various analysis procedures were compared such as Oliver&Pharr and nominal hardness-based methods,which require area function of the indenter,and other methods based on energy,displacement,contact depth,and contact stiffness,which do not require calibration of the indenter.Elastic recovery of the imprint by the Knoop indenter was also utilized to evaluate elastic moduli of brittle solids.Expressions relating HIT/Er and dimensionless nanoindentation variables(e.g.,the ratio of elastic work over total work and the ratio of permanent displacement over maximum displacement)are found to be nonlinear rather than linear for brittle solids.The plastic hardness Hp of brittle solids(except traditional glasses)extracted based on Er is found to be proportional to E_(r)√H_(IT).
基金supported by the Project of Key R&D Program of Shandong Province(2023CXGC010712).Geoffrey I.N.
文摘In this work,a novel electrochemical sensor based on covalent organic framework@carbon black@molecularly imprinted polydopamine(COF@CB@MPDA)was developed for selective recognition and determination of ciprofloxacin(CF).COF@CB@MPDA possessed good water dispersibility and was synthesized by the selfpolymerization of dopamine under alkaline conditions in the presence of the COF,CB and CF.The high surface area COF enhanced the adsorption of CF,whilst CB gave the composites high electrical conductivity to improve the sensitivity of the proposed COF@CB@MPDA/glassy carbon electrode(GCE)sensor.The specific recognition of CF by COF@CB@MPDA involved hydrogen bonding and van der Waals interactions.Under optimized conditions,the sensor showed a good linear relationship with CF concentration over the range of 5.0×10^(–7)and 1.0×10^(–4)mol/L,with a limit of detection(LOD)of 9.53×10^(–8)mol/L.Further,the developed sensor exhibited high selectivity,repeatability and stability for CF detection in milk and milk powders.The method used to fabricate the COF@CB@MPDA/GCE sensor could be easily adapted for the selective recognition and detection of other antibacterial agents and organic pollutants in the environment.
文摘Gestational diabetes mellitus(GDM)is increasingly recognized not only for its immediate obstetric complications but also for its long-term metabolic consequences in both mothers and their offspring.Traditionally,research has emphasized the roles of pancreaticβ-cell dysfunction and placental dysregulation in GDM.However,emerging evidence highlights the maternal liver as a central metabolic hub during pregnancy coordinating glucose,lipid,and hormonal adaptations essential for fetal development.This review synthesizes current findings on how GDM disrupts the maternal liver’s adaptive roles,transforming it from a metabolic coordinator into a source of maladaptive endocrine,inflammatory,and nutrient signals.It outlines key mechanistic pathways through which maternal hepatic dysfunction may increase offspring susceptibility to non-alcoholic fatty liver disease and type 2 diabetes mellitus.These include hepatokine dysregulation,altered lipid metabolism,impaired insulin signaling,inflammatory and oxidative stress pathways,and epigenetic and transcriptomic reprogramming.In addition,it explores novel axes such as the gut-liver-placenta interplay,bile acid signaling disruptions,endoplasmic reticulum stress responses,and extracellular vesiclemediated communication.By reframing the maternal liver’s role in GDM pathophysiology,this review identifies critical windows for early clinical intervention and highlights the potential for liver-focused strategies to prevent the intergenerational transmission of metabolic disease.
基金supported by STI2030-Major Projects(2023ZD04069,Z231100003723004)the National Science Fund for Distinguished Young Scholars(32425041)the Chinese Universities Scientific Fund(2024TC165).
文摘Maize(Zea mays L.)is a globally significant crop essential for food,feed,and bioenergy production.The maize kernel,serving as a primary sink for starch,proteins,lipids,and essential micronutrients,is crucial for enhancing maize yield and quality.Previous studies have established the critical role of Polycomb Repressive Complex 2(PRC2)in regulating kernel development.In this study,we applied a reverse genetics approach to investigate the role of ZmFIE1,the homolog of the PRC2 complex component Extra sex combs(Esc),in maize development.The functional loss of ZmFIE1 significantly reduces embryo size in the early stage but has a relatively small impact on mature kernels.Integrating transcriptional and metabolomic profiling suggests that ZmFIE1 is involved in regulating nutrient balance between the endosperm and embryo.In addition,we demonstrate that ZmFIE1 is maternally expressed,and that the maternal inheritance of the fie1 allele significantly affects the imprinting status of paternally imprinted genes.Overall,our results suggest that ZmFIE1 is a key gene involved in the modulation of embryo development via regulating genomic imprinting and nutrient balance between embryo and endosperm,which provides new insights into the regulation mechanism underlying kernel development.
文摘BACKGROUND Silver-Russell syndrome(SRS)is a clinically heterogeneous entity characterized by intrauterine and postnatal growth restriction,relative macrocephaly at birth,distinct facial features,and body asymmetry combined with other malformations.CASE SUMMARY Herein,we describe four individuals with SRS,focusing on their prenatal phenotype,postnatal presentation,diagnosis,and management.All cases had a typical phenotype,including postnatal growth failure,short stature(chronic malnutrition),and protruding forehead.Individually,they presented with feeding difficulties,leg length discrepancy,triangular face,or relative macrocephaly at birth,and each one exhibited distinct SRS features,including motor and/or speech delay,experiencing frequent hypoglycemic episodes.The fact that each patient exhibited a different combination of clinical findings underlines the heterogeneity of the syndrome.CONCLUSION SRS is diagnosed clinically.However,only 60%of cases are genetically confirmed,while most are sporadic.Although SRS is a well-described syndrome,a delayed diagnosis can have grave consequences on a child’s growth.Recombinant human growth hormone treatment is often initiated shortly after the diagnosis.The follow-up requires a multidisciplinary approach.
基金supported by the Fuxiaquan Collaborative Innovation Platform(No.K30001)Major Scientific Research Program for Young and Middle-aged Health Professionals of Fujian Province,China(No.2022ZQNZD007)Youth Innovation Technology Project of Higher School in Shandong Province(Food Nanotechnology Innovation Team)。
文摘Quantitative determination of tetracycline(TC)in environment and foods is of great importance,as excessive residues might have negative effects on human health and environmental risks.Herein,a selfpowered molecularly imprinted photoelectrochemical(PEC)sensor based on the Zn O/C photoanode and the Fe-doped CuBi_(2)O_(4)(CBFO)photocathode is developed for the sensitive detection of TC.The photocathodic current can be amplified by the efficient electron transfer caused by the Fermi energy level gap between the photoanode and photocathode.Furthermore,molecularly imprinted polymers(MIPs)at photocathode can selectivity identify the TC templates and thus improve the specificity.Under the optimal conditions,the sensor has a linear range of 10^(-2)-1.0×10^(5) nmol/L,and a limit of detection(LOD)of 0.007 nmol/L(S/N=3).More crucially,the milk sample detection is carried out using the as-prepared sensor,and the outcome is satisfactory.The research gives us a novel sensing platform for quick and accurate antibiotic(like TC)in environment and food monitoring.
基金Project(41271332)supported by the National Natural Science Foundation of ChinaProject(2010YBB186)supported by the Social Science Foundation of Hunan Province,Chian
文摘Methacrylic acid was used together with a molecular imprinting technique to modify chitosan. In addition, the adsorption kinetics and adsorption isotherms were recorded and the results were analyzed to investigate reparative adsorption for Cr（VI） from the polluted Xiangjiang River water. A comparative X-ray analysis shows that the degree of crystallization in the imprinted polymer was significantly weakened, the area of the non-crystalline region was larger. There were more adsorption sites in the imprinted polymer, and the adsorption capacity towards Cr（VI） was increased. The adsorption capacity of the imprinted polymer towards Cr（VI） increased with time and reaches saturation after 8 h. The optimal adsorption time was 4-8 h after the adsorption starting and the optimal pH value for the solution was in the range of 4.5-7.5. When the chitosan reaches saturation, the adsorption capacity achieves a state of equilibrium, and the maximum Cr（VI） extraction rate reaches 33.7%. Moreover, the adsorption capacity of the imprinted polymer towards Cr（VI） increases with increasing chitosan concentration. In this situation, the Cr（VI） extraction rate shows little variation, and the maximum removal rate can reach 98.3%. Furthermore, the Cr（VI） extraction rate increases with an increase in the degree of deacetylation in the chatoyant and chitosan, with the best adsorption effect corresponding to 90% deacetylation. Fitting the adsorption data to the quasi first- and second-order kinetic models yields correlation coefficients of 0.9013 and 0.9875, respectively. The corresponding rate constants for the two models are 0.0091 min-1 and 7.129 g/（mg.min）, respectively. Hence, the adsorption using Cr（VI）-imprinted chitosan is more consistent with the second-order kinetics. Comparing the data to Freundlich and Langrnuir adsorption isotherms shows that the latter has a better linear fit and a maximum adsorption capacity of 15.784 mg/g.
基金supported by the Key Laboratory for Rare Disease of Shandong Province
文摘Solid phase extraction （SPE） is a widely used sample pretreatment method for separation, purification and enrichment, which has been established due to its significant advantages of time-saving, low consumption of solvent, high enrichment factor, high accuracy, etc. In recent years, a variety of new SPE methods such as molecularly imprinted solid phase extraction （MISPE）, magnetic solid phase extraction （MSPE）, solid phase micro-extraction （SPME）, etc., which are superior to the conventional SPE, have been developed and been widely applied to food, drugs, and environmental monitoring. In this paper, the basic principles and methods of SPE and its new applications in different areas are reviewed.
文摘Prader-Willi Syndrome （PWS） is a genetic disorder that is difficult to detect, particularly at an early age. PWS is caused by disruption of normal, epigenetically controlled gene function in the chromosome 15q11-q13 region. Clinical symptoms are difficult to diagnose in infants and only become clearer at later ages as the patients develop hyperphagia and morbid obesity. Molecular genetic tests are able to definitively diagnose PWS and allow early diagnosis of the syndrome. High resolution cytogenetic testing, methylation-specific PCR （MS-PCR）, and linkage analysis are routinely used to diagnose PWS. To establish a linkage analysis method for Chinese patients, this study identified a useful set of STR markers in the typical PWS deletion and adjacent area, for linkage analysis in two Chinese families with PWS offspring. Using this method, the authors confn＇rned that one patient had a paternal deletion in chromosome 15q 11-q 13 and the other patient had maternal uniparental heterodisomy of chromosome 15. MS -PCR and high resolution chromosome G-banding also confirmed this diagnosis. This linkage analysis method can detect both deletion and uniparental disomy, thus providing valuable information for genetic counseling and the opportunity to analyze the relationship between the genotype and phenotype of PWS.
基金Supported by National Natural Science Foundation of China(31660402)Industry Technological System Construction Project of Department of Agriculture of Yunnan ProvinceFund for Workstation of Academician Guan Chunyun from Department of Science and Technology of Yunnan Province~~
文摘Genomic imprinting is the epigenetic phenomenon by which certain genes are expressed in a parent-of-origin-specific manner, and was first discovered in mammalian embryos. Recent studies have shown that it also occurs in developing plant seeds, and is now becoming a hot topic of biology of plant seed development. According to the previous studies on imprinted genes, imprinting mechanism and their roles in plant seed development, the current progress of genomic imprinting in plant seed development was summarized and possible strategies were proposed to deal with the problems, which could provide helpful information for further research.
