A molecularly imprinted electrochemical sensor was prepared based on poly folic acid（PFA） for rapid detection of barbital（BAR）. The PFA membrane was obtained via directly electropolymerization technique on the sur...A molecularly imprinted electrochemical sensor was prepared based on poly folic acid（PFA） for rapid detection of barbital（BAR）. The PFA membrane was obtained via directly electropolymerization technique on the surface of chemically modified Au electrode（Au/CME） by means of cyclic voltammetry（CV） in the potential range between-0.4 and 1.0 V in phosphate buffer solution（PBS） pH 7.04. The molecularly imprinted polymers（MIP） membrane was synthesized with BAR as template molecules and folic acid（FA） as the functional monomer. The performance and surface feature of the proposed imprinted sensor were investigated using CV, differential pulse voltammetry（DPV）, electrochemical impedance spectroscopy（EIS） and scanning electron microscope（SEM）. Under the optimized conditions, the peak current decrease（ΔIp） was proportional to the BAR concentration in the range of 1.00×10^-7-1.00×10^-4 mol/L（R^2=0.998 2） with a detection limit（S/N=3） of 4.65×10^-8 mol/L. The results indicated that the imprinted sensor exhibited an excellent selectivity for BAR and it was successfully used to determine BAR in real samples with recoveries of 94.7%-106.2% by using the standard addition method.展开更多
This article describes the development of a molecularly imprinted polymers(MIPs)based sensor for the determination of vanillylmandelic acid(VMA),which is an important biomarker for tumours like neuroblastoma and pheoc...This article describes the development of a molecularly imprinted polymers(MIPs)based sensor for the determination of vanillylmandelic acid(VMA),which is an important biomarker for tumours like neuroblastoma and pheochromocytoma.Only bulk synthesis-based MIPs sensors have been reported so far for VMA.The proposed sensor,which utilizes poly(o-Amino phenol)(pOAP)for the MIPs fabrication and(Taurine-Glutamic acid)copolymer(p(TR-GA))to enhance sensitivity,possesses notable sensing characteristics.Synthesis and deposition of the polymers on the glassy carbon electrode has been carried out using electrochemical technique.Ample interactions between OAP monomer units and VMA molecules resulted in the formation of imprints with good recognition ability.Imprinting factor associated with the sensor has been found to be 5.0 and the determination of VMA is possible in the linear range of 7.00×10^(-5)-8.00×10^(-7)mol/L.The aim to eliminate the interference from coexisting species of VMA could be favourably achieved through this MIPs sensor.Moreover,the sensor possesses superior performance over the reported bulk synthesis-based MIPs sensors for VMA.展开更多
Molecularly imprinted polymers are generated by curing a cross-linked polymer in the presence of a template. During the curing process, noncovalent bonds form between the polymer and the template. The interaction site...Molecularly imprinted polymers are generated by curing a cross-linked polymer in the presence of a template. During the curing process, noncovalent bonds form between the polymer and the template. The interaction sites for the noncovalent bonds become "frozen" in the cross-linking polymer and maintain their shape even after the template is removed. The resulting cavities reproduce the size and shape of the template and can selectively reincorporate the template when a mixture containing it flows over the imprinted surface. In the last few decades the field of molecular imprinting has evolved from being able to selectively capture only small molecules to dealing with all kinds of samples. Molecularly imprinted polymers (MIPs) have been generated for analytes as diverse as metal ions, drug molecules, environmental pollutants, proteins and viruses to entire cells. We review here the relatively new field of surface imprinting, which creates imprints of large, biologically relevant templates. The traditional bulk imprinting, where a template is simply added to a prepolymer before curing, cannot be applied if the analyte is too large to diffuse from the cured polymer. Special methods must be used to generate binding sites only on a surface. Those techniques have solved crucial problems in separation science as well as chemical and biochemical sensing. The implementation of imprinted polymers into microfluidic chips has greatly improved the applicability of microfluidics. We present the latest advances and different approaches of surface imprinting and their applications for microfluidic devices.展开更多
基金Supported by the Natural Science Foundation of Shanxi Province(20001057)
文摘A molecularly imprinted electrochemical sensor was prepared based on poly folic acid（PFA） for rapid detection of barbital（BAR）. The PFA membrane was obtained via directly electropolymerization technique on the surface of chemically modified Au electrode（Au/CME） by means of cyclic voltammetry（CV） in the potential range between-0.4 and 1.0 V in phosphate buffer solution（PBS） pH 7.04. The molecularly imprinted polymers（MIP） membrane was synthesized with BAR as template molecules and folic acid（FA） as the functional monomer. The performance and surface feature of the proposed imprinted sensor were investigated using CV, differential pulse voltammetry（DPV）, electrochemical impedance spectroscopy（EIS） and scanning electron microscope（SEM）. Under the optimized conditions, the peak current decrease（ΔIp） was proportional to the BAR concentration in the range of 1.00×10^-7-1.00×10^-4 mol/L（R^2=0.998 2） with a detection limit（S/N=3） of 4.65×10^-8 mol/L. The results indicated that the imprinted sensor exhibited an excellent selectivity for BAR and it was successfully used to determine BAR in real samples with recoveries of 94.7%-106.2% by using the standard addition method.
文摘This article describes the development of a molecularly imprinted polymers(MIPs)based sensor for the determination of vanillylmandelic acid(VMA),which is an important biomarker for tumours like neuroblastoma and pheochromocytoma.Only bulk synthesis-based MIPs sensors have been reported so far for VMA.The proposed sensor,which utilizes poly(o-Amino phenol)(pOAP)for the MIPs fabrication and(Taurine-Glutamic acid)copolymer(p(TR-GA))to enhance sensitivity,possesses notable sensing characteristics.Synthesis and deposition of the polymers on the glassy carbon electrode has been carried out using electrochemical technique.Ample interactions between OAP monomer units and VMA molecules resulted in the formation of imprints with good recognition ability.Imprinting factor associated with the sensor has been found to be 5.0 and the determination of VMA is possible in the linear range of 7.00×10^(-5)-8.00×10^(-7)mol/L.The aim to eliminate the interference from coexisting species of VMA could be favourably achieved through this MIPs sensor.Moreover,the sensor possesses superior performance over the reported bulk synthesis-based MIPs sensors for VMA.
文摘Molecularly imprinted polymers are generated by curing a cross-linked polymer in the presence of a template. During the curing process, noncovalent bonds form between the polymer and the template. The interaction sites for the noncovalent bonds become "frozen" in the cross-linking polymer and maintain their shape even after the template is removed. The resulting cavities reproduce the size and shape of the template and can selectively reincorporate the template when a mixture containing it flows over the imprinted surface. In the last few decades the field of molecular imprinting has evolved from being able to selectively capture only small molecules to dealing with all kinds of samples. Molecularly imprinted polymers (MIPs) have been generated for analytes as diverse as metal ions, drug molecules, environmental pollutants, proteins and viruses to entire cells. We review here the relatively new field of surface imprinting, which creates imprints of large, biologically relevant templates. The traditional bulk imprinting, where a template is simply added to a prepolymer before curing, cannot be applied if the analyte is too large to diffuse from the cured polymer. Special methods must be used to generate binding sites only on a surface. Those techniques have solved crucial problems in separation science as well as chemical and biochemical sensing. The implementation of imprinted polymers into microfluidic chips has greatly improved the applicability of microfluidics. We present the latest advances and different approaches of surface imprinting and their applications for microfluidic devices.
