As a typical bioflavonoid,diosmetin is desirable in the field of natural medicine,healthy food,and cosmetics by anti-cancer,antibacterial,antioxidant,estrogen-like and anti-inflammatory activities,and it comes from a ...As a typical bioflavonoid,diosmetin is desirable in the field of natural medicine,healthy food,and cosmetics by anti-cancer,antibacterial,antioxidant,estrogen-like and anti-inflammatory activities,and it comes from a wide range of sources in traditional Chinese medicine like spider fragrance,spearmint and chrysanthemum,as well as in Citrus fruit.However,traditional analytical methods such as silica gel column chromatography face multiple challenges in the selective extraction of diosmetin from biological materials and traditional Chinese medicinal materials.Therefore,it is urgent to develop a new type of absorbent with high efficiency,recyclability and good specificity to diosmetin.In this investigation,a magnetic surface molecularly imprinted polymer(labeled as Diosmetin/SMIPs)was synthesized employing magnetic nanoparticles as the carrier and 4-vinylpyridinyl(4-VP)as the functional monomer by surface imprinting technology.The functional monomer was screened by the binding energy(△E)between functional monomers and template molecules via computational simulation.The Diosmetin/SMIPs had a high level of specific recognition and adsorption capability towards diosmetin with a 20.25 mg g^(-1) adsorption capacity and an imprinting factor(IF)of 2.28.Additionally,it demonstrated excellent regeneration performance with 8 adsorption/desorption cycles.In addition,91.20%-94.16% of spiked diosmetin was recovered from the lemon peel samples.The strategy of constructing Diosmetin/SMIPs based on computational simulation can effectively enhance the specific adsorption performance of diosmetin.Meanwhile,Diosmetin/SMIPs synthesized by imprinting polymerization showed excellent anti-interference and reusability,and realized efficient targeted extraction of diosmetin from lemon peel samples.The results of this investigation provide a promising adsorbent for selective enrichment of diosmetin from Citrus fruit and complicated materials.展开更多
In this work,by combining the specific selectivity of molecularly imprinted polymer(MIP)with a simple and sensitive electrochemiluminescence(ECL)detection method,a molecularly imprinted ECL sensor for kanamycin(KA)was...In this work,by combining the specific selectivity of molecularly imprinted polymer(MIP)with a simple and sensitive electrochemiluminescence(ECL)detection method,a molecularly imprinted ECL sensor for kanamycin(KA)was developed.The MIP film was synthesized on the surface of gold electrode via electrochemical polymerization,using pyrrole(PY)as the functional monomer and KA as the template molecule.The commonly used luminescent reagent Ru(bpy)32+was employed as the ECL probe,and its co-reagent tripropylamine(TPA)was added to enhance the detection sensitivity of the sensor.Quantitative analysis of KA was performed by evaluating the difference in ECL responses between MIP film electrodes after KA removal and KA rebinding.The sensor exhibited high selectivity,good reproducibility,and stability toward KA,with a linear range of 5.00×10^(-8) to 1.00×10^(-5) mol·L^(-1) and a limit of detection of 1.67×10^(-8) mol·L^(-1)(S/N=3).This work would provide a new idea for the development of simple and sensitive molecularly imprinted ECL sensors.展开更多
Molecularly imprinted polymers(MIPs)are a kind of synthetic receptors possessing wide application prospects in proteins recognition.However,there are still great challenges in proteins imprinting due to their large si...Molecularly imprinted polymers(MIPs)are a kind of synthetic receptors possessing wide application prospects in proteins recognition.However,there are still great challenges in proteins imprinting due to their large size and easy conformation change.In this study,we explored epitope-oriented MIP based on host-vip interaction(hg-MIP)and constructed a novel hg-MIP-SERS(surface-enhanced Raman scatting)approach for efficiently recognizing the terminal epitopes of neuron-specific enolase(NSE),a well-known disease biomarker for small cell lung cancer,neuroblstom,and Alzheimer's disease.The C-and N-terminal epitopes of NSE were modified with 4-(phenylazo)benzoic acid,then they were used as the templates and immobilized onβ-cyclodextrin-functionalized substrates.The imprinted layer was formed by polymerization of various functional monomers.Combined with SERS detection,an antibody-free sandwich assay based on hg-MIP was successfully used to detect the concentration of NSE in human serums,with the advantages of simple operation,small sample volume(5μL),wide linear range(1–10^(4)ng/m L)and a limit of detection as low as 0.01 ng/m L.The developed epitope-oriented hg-MIP-SERS approach can also be extended to other proteins,expanding the imprinting method of proteins,and has a broad development space in the field of protein separation and detection.展开更多
Bromocresol green(BCG)and malachite green(MG)are water-soluble toxic organic dyes with adverse health and environmental implications.This study presented a conjugate imprinted adsorbent(CIA)synthesized by incorporatin...Bromocresol green(BCG)and malachite green(MG)are water-soluble toxic organic dyes with adverse health and environmental implications.This study presented a conjugate imprinted adsorbent(CIA)synthesized by incorporating trimethoprim vanillin ligand into a highly crosslinked polymer,designed for the efficient removal of BCG and MG from wastewater.Characterization of CIA involved X-ray powder diffraction,Fourier transform infrared,and scanning electron microscopic analyses.Batch adsorption processes were conducted to evaluate the adsorption characteristics of CIA,with focuses on the effects of contact time,initial dye concentration,pH,and temperature.The molecularly imprinted polymers(MIPs)achieved removal efficiencies of 99.27%and 98.99%at equilibrium for BCG and MG adsorption,respectively.The non-imprinted polymers(NIPs)demonstrated BCG and MG adsorption efficiencies of 51.52%and 62.90%at equilibrium,respectively.Kinetic and isotherm models were employed to elucidate the BCG and MG adsorption mechanisms.The thermodynamic results indicated non-spontaneous and spontaneous reactions for BCG and MG adsorption on MIPs under the examined temperature conditions.The adsorbent exhibited sustained high removal efficiency through five reuse cycles,with no apparent reduction in adsorption performance.Validation of the adsorbent using real textile wastewater samples achieved BCG and MG removal efficiencies of 85.5%-87.5%.The adsorbent outperformed previously reported materials in BCG and MG adsorption.The synthesized CIA is a promising adsorbent for BCG and MG dye removal,contributing to water sustainability.展开更多
In this work,a novel electrochemical sensor based on covalent organic framework@carbon black@molecularly imprinted polydopamine(COF@CB@MPDA)was developed for selective recognition and determination of ciprofloxacin(CF...In this work,a novel electrochemical sensor based on covalent organic framework@carbon black@molecularly imprinted polydopamine(COF@CB@MPDA)was developed for selective recognition and determination of ciprofloxacin(CF).COF@CB@MPDA possessed good water dispersibility and was synthesized by the selfpolymerization of dopamine under alkaline conditions in the presence of the COF,CB and CF.The high surface area COF enhanced the adsorption of CF,whilst CB gave the composites high electrical conductivity to improve the sensitivity of the proposed COF@CB@MPDA/glassy carbon electrode(GCE)sensor.The specific recognition of CF by COF@CB@MPDA involved hydrogen bonding and van der Waals interactions.Under optimized conditions,the sensor showed a good linear relationship with CF concentration over the range of 5.0×10^(–7)and 1.0×10^(–4)mol/L,with a limit of detection(LOD)of 9.53×10^(–8)mol/L.Further,the developed sensor exhibited high selectivity,repeatability and stability for CF detection in milk and milk powders.The method used to fabricate the COF@CB@MPDA/GCE sensor could be easily adapted for the selective recognition and detection of other antibacterial agents and organic pollutants in the environment.展开更多
Maize(Zea mays L.)is a globally significant crop essential for food,feed,and bioenergy production.The maize kernel,serving as a primary sink for starch,proteins,lipids,and essential micronutrients,is crucial for enhan...Maize(Zea mays L.)is a globally significant crop essential for food,feed,and bioenergy production.The maize kernel,serving as a primary sink for starch,proteins,lipids,and essential micronutrients,is crucial for enhancing maize yield and quality.Previous studies have established the critical role of Polycomb Repressive Complex 2(PRC2)in regulating kernel development.In this study,we applied a reverse genetics approach to investigate the role of ZmFIE1,the homolog of the PRC2 complex component Extra sex combs(Esc),in maize development.The functional loss of ZmFIE1 significantly reduces embryo size in the early stage but has a relatively small impact on mature kernels.Integrating transcriptional and metabolomic profiling suggests that ZmFIE1 is involved in regulating nutrient balance between the endosperm and embryo.In addition,we demonstrate that ZmFIE1 is maternally expressed,and that the maternal inheritance of the fie1 allele significantly affects the imprinting status of paternally imprinted genes.Overall,our results suggest that ZmFIE1 is a key gene involved in the modulation of embryo development via regulating genomic imprinting and nutrient balance between embryo and endosperm,which provides new insights into the regulation mechanism underlying kernel development.展开更多
This work develops a protein imprinted nanosphere with varied recognition specificity for bovine serum albumin(BSA)and lysozyme(Lyz)under different UV light through a gradient dual crosslinked imprinting strategy(i.e....This work develops a protein imprinted nanosphere with varied recognition specificity for bovine serum albumin(BSA)and lysozyme(Lyz)under different UV light through a gradient dual crosslinked imprinting strategy(i.e.,covalent crosslinking and dynamic reversible crosslinking).The imprinting cavities are initially constructed using irreversible covalent crosslinking to specifically recognize BSA,and then the coumarin residues in the imprinting cavities are crosslinked under 365 nm UV light to further imprint Lyz,because Lyz has smaller size than BSA.Since the photo-crosslinking of coumarin is a reversible reaction,the imprinting cavities of Lyz can be de-crosslinked under 254 nm UV light and restore the imprinting cavities of BSA.Moreover,the N-isopropyl acrylamide(NIPAM)and pyrrolidine residues copolymerized in the polymeric surface of the nanospheres are temperature-and p H-responsive respectively.Therefore,the protein rebinding and release behaviors of the nanospheres are controlled by external temperature and p H.As a result,the materials can selectively separate BSA from real bovine whole blood and Lyz from egg white under different UV light.This study may provide a new strategy for construction of protein imprinted materials with tunable specificity for different proteins.展开更多
The characterization of these molecularly imprinted polymers is essential to understanding their binding dynamics and structural properties. Through the analysis of the current research, it is found that there are ove...The characterization of these molecularly imprinted polymers is essential to understanding their binding dynamics and structural properties. Through the analysis of the current research, it is found that there are overlaps in the methods used by scholars. The Langmuir equation is frequently applied to model the adsorption isotherms of MIPs, providing critical insight into the capacity and affinity of the binding sites. Infrared Spectroscopy (IR) plays a crucial role in identifying the functional groups involved in the imprinting process and confirming the successful formation of specific binding sites. UV-visible spectrophotometry is employed to monitor the absorption characteristics of the polymers, offering data on the interactions between the template molecules and the polymer matrix. Transmission Electron Microscopy (TEM) provides detailed visualization of the internal structure of MIPs at the nanoscale, revealing the morphology and size of the imprinted cavities. Thermogravimetric Analysis (TGA) assesses the thermal stability and composition of the polymers, identifying decomposition patterns that are indicative of the material’s robustness under different conditions. Finally, the Laser Particle Size Analyzer is used to measure the size distribution of the polymer particles, which is critical for determining the uniformity and efficiency of the imprinting process. The six characterization methods discussed in this paper provide a comprehensive understanding of MIP, and it is hoped that in the future, more optimized design solutions will emerge and their applications in various fields will be enhanced.展开更多
There is an urgent imperative to discover practical and efficacious artificial catalysts for the expeditious decontamination of toxic organophosphates. Herein, we propose a novel molecularly imprinted approach utilizi...There is an urgent imperative to discover practical and efficacious artificial catalysts for the expeditious decontamination of toxic organophosphates. Herein, we propose a novel molecularly imprinted approach utilizing electrospun fiber scaffolds. Specifically, an amidoxime-based functional polymer (PMAOX) has been synthesized, which contains amidoxime groups that can act as nucleophiles and ligands for the formation of catalytic active sites. This polymer was blended with polyacrylonitrile (PAN), a well-processable material, to prepare a nanofiber mat using electrospinning techniques. Then, the amidoxime side groups on fiber surface were further cross-linked after the molecular coordination of templates to complete the molecularly imprinting process. This approach can not only enhance the content of functional molecularly imprinted polymers without affecting structural stability, but also combines surface MI technology to fully expose and leverage the advantages of active sites. The as prepared molecularly imprinted electrospun nanofibers MIF-Zn-PMAOX/PAN-6/4 catalyzes the degradation of paraoxon with a half-life of 32 min, and MIF-Ag-PMAOX/PAN catalyzes the degradation of parathion with a half-life of 18 min. The maximum catalytic rate evidence rate enhancements nearly 3700-fold of the self-hydrolysis. Thus, the mono-amidoxime based molecularly imprinted fibers demonstrate the versatility and superiority as self-detoxifying for organophosphates.展开更多
The early development of the endosperm is crucial for balancing the allocation of maternal nutrients to offspring.This process is believed to be evolutionarily associated with genomic imprinting,resulting in parentall...The early development of the endosperm is crucial for balancing the allocation of maternal nutrients to offspring.This process is believed to be evolutionarily associated with genomic imprinting,resulting in parentally biased allelic gene expression.Beyond Fertilization Independent Seed(FIS)genes,the number of imprinted genes involved in early endosperm development and seed size determination remains limited.This study introduces early endosperm-expressed HAIKU(IKU)downstream Candidate F-box 1(ICF1)and ICF2 as maternally expressed imprinted genes(MEGs)in Arabidopsis thaliana.Although these genes are also demethylated by DEMETER(DME)in the central cell,their activation differs from the direct DME-mediated activation seen in classical MEGs such as the FIS genes.Instead,ICF maternal alleles carry pre-established hypomethylation in their promoters,priming them for activation by the WRKY10 transcription factor in the endosperm.On the contrary,paternal alleles are predominantly suppressed by CG methylation.Furthermore,we find that ICF genes partially contribute to the small seed size observed in iku mutants.Our discovery reveals a two-step regulatory mechanism that highlights the important role of conventional transcription factors in the activation of imprinted genes,which was previously not fully recognized.Therefore,the mechanism provides a new dimension to understand the transcriptional regulation of imprinting in plant reproduction and development.展开更多
A pre-treatment methodology for clenbuterol hydrochloride （CLEN） isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers （MIPs）. CLEN-imprinted pol-...A pre-treatment methodology for clenbuterol hydrochloride （CLEN） isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers （MIPs）. CLEN-imprinted pol- ymers were synthesized by the combined use of ally-β-cyclodextrin （ally-13-CD） and methacrylic acid （MAA）, allyl-β-CD and acrylonitrile （AN）, and allyl-β-CD and methyl methacrylate （MMA） as the binary functional monomers. MAA-linked allyl-β-CD MIPs （M-MAA） were characterized by Fourier transform-infrared （FT-IR） spectroscopy and a scanning electron microscope （SEM）. Based upon the results, M-MAA polymers generally proved to be an excellent selective extraction compared to its references： AN-linked allyl-β-CD MIPs （M-AN） and MMA-linked allyl-β-CD MIPs （M-MMA）. M-MAA polymers were eventually chosen to run through a molecularly imprinted solid-phase extraction （MISPE） micro-column to enrich CLEN residues spiked in pig livers. A high recovery was achieved, ranging from 91.03% to 96.76% with relative standard deviation （RSD） ≤4.45%.展开更多
Heavy metal ion is one of the major environmental pollutants.In this study,a Cu(Ⅱ)ions imprinted magnetic chitosan beads are prepared to use chitosan as functional monomer,Cu(Ⅱ)ions as template,Fe_(3)O_(4) as magnet...Heavy metal ion is one of the major environmental pollutants.In this study,a Cu(Ⅱ)ions imprinted magnetic chitosan beads are prepared to use chitosan as functional monomer,Cu(Ⅱ)ions as template,Fe_(3)O_(4) as magnetic core and epichlorohydrin and glutaraldehyde as crosslinker,which can be used for removal Cu(Ⅱ)ions from wastewater.The kinetic study shows that the adsorption process follows the pseudosecond-order kinetic equations.The adsorption isotherm study shows that the Langmuir isotherm equation best fits for the monolayer adsorption processes.The selective adsorption properties are performed in Cu(Ⅱ)/Zn(Ⅱ),Cu(Ⅱ)/Ni(Ⅱ),and Cu(Ⅱ)/Co(Ⅱ)binary systems.The results shows that the ⅡMCD has a high selectivity for Cu(Ⅱ)ions in binary systems.The mechanism of ⅡMCD recognition Cu(Ⅱ)ions is also discussed.The results show that the ⅡMCD adsorption Cu(Ⅱ)ions is an enthalpy controlled process.The absolute value of DH(Cu(Ⅱ))and DS(Cu(Ⅱ))is greater than DH(Zn(Ⅱ),Ni(Ⅱ),Co(Ⅱ))and DS(Zn(Ⅱ),Ni(Ⅱ),Co(Ⅱ)),respectively,this indicates that the Cu(Ⅱ)ions have a good spatial matching with imprinted holes on ⅡMCD.The FTIR and XPS also demonstrates the strongly combination of function groups on imprinted holes in the suitable space position.Finally,the ⅡMCD can be regenerated and reused for 10 times without a significantly decreasing in adsorption capacity.This information can be used for further application in the selective removal of Cu(Ⅱ)ions from industrial wastewater.展开更多
Dummy molecularly imprinted polymers （DMIPs） for 4-（methylnitrosamino）-1-（3-pyridyl）-1-butanol （NNAL） were produced using three structural analogues as dummy template molecules. The chosen analogues were 4-（a...Dummy molecularly imprinted polymers （DMIPs） for 4-（methylnitrosamino）-1-（3-pyridyl）-1-butanol （NNAL） were produced using three structural analogues as dummy template molecules. The chosen analogues were 4-（acetymethylamino）-1-（3-pyridyl）-butanol, 4- （methylamino）-1-（3-pyridyl）-1-butanol, and 1-（3-pyridyl）-1,4,-butanediol. The molecular recognition characteristics of the produced polymers were evaluated by X-ray photoelec- tron spectroscopy （XPS） and Fourier transform infrared spectroscopy （FT-IR）. Interactions between NNAL and methacrylic acid should be cooperative hydrogen bonds while the ni- trogen atom of the pyridine ring and the oxygen atom of the nitroso group in NNAL are two of the hydrogen-bond acceptors. It was further demonstrated that DMIP synthesized by 4-（acetymethylamino）-1-（3-pyridyl）-butanol had the best binding performance by XPS and FT-IR. Then dummy molecularly imprinted solid phase extraction （DMISPE） was developed for the determination of the analyte using the hit polymer as the sorbing material. Under optimal conditions, the recovery of NNAL dissolved in standard solution reached 93%. And the investigated polymer exhibited much higher binding of NNAL when nicotine was acted as the competitive molecule. Also the proposed method was applied to the measurement of NNAL spiked in blank urine samples with recoveries ranging from 87.2% to 101.2%.展开更多
A new bovine serum albumin （BSA） surface imprinting method was developed by the incorporation of quantum dots （QDs） into molecularly imprinted polymers （MIP）, which can offer shape selectivity. Preparation and a...A new bovine serum albumin （BSA） surface imprinting method was developed by the incorporation of quantum dots （QDs） into molecularly imprinted polymers （MIP）, which can offer shape selectivity. Preparation and adsorption conditions were optimized. Physical appearance of the QDs and QDs-MIP particles was illustrated by scanning electron microscope images. Photoluminescence emission of CdS was quenched when rebinding of the template.The quenching of photoluminescence emissions is presumably due to the fluorescence resonance energy transfer between quantum dots and BSA template molecules. The adsorption is compiled with Langmuir isotherm, and chemical adsorption is the rate-controlling step.The maximum adsorption capacity could reach 226.0 mg/g, which is 142.4 mg/g larger than that of undoped BSA MIP. This study demonstrates the validity of QDs coupled with MIP technology for analyzing BSA.展开更多
Parthenogenetic embryonic stem （pES） cells provide a valuable in vitro model system for studying the molecular mechanisms that underlie genomic imprinting. However, the pluripotency of pES cells and the expression p...Parthenogenetic embryonic stem （pES） cells provide a valuable in vitro model system for studying the molecular mechanisms that underlie genomic imprinting. However, the pluripotency of pES cells and the expression profiles of paternally expressed imprinted genes have not been fully explored. In this study, three mouse pES cell lines were established and the differentiation potential of these cells in extended culture was evaluated. The undifferentiated cells had a normal karyotype and homozygous genome, and expressed ES-cell-specific molecular markers. The cells remained undifferentiated after more than 50 passages and exhibited pluripotent differentiation capacity. All three lines of the established ES cells produced teratomas; two lines of ES cells produced chimeras and germline transmission. Furthermore, activation of the paternally expressed imprinted genes Snrpn, U2afl-rsl, Peg3, Impact, Zfp127, Dlkl and Mest in these cells was detected. Some paternally expressed imprinted genes were found to be expressed in the blastocyst stage of parthenogenetically activated embryos in vitro and their expression level increased with extended pES cell culture. Furthermore, our data show that the activation of these paternally expressed imprinted genes in pES cells was associated with a change in the methylation of the related differentially methylated regions. These findings provide direct evidence for the pluripotency of pES cells and demonstrate the association between the DNA methylation pattern and the activa- tion of paternally expressed imprinted genes in pES cells. Thus, the established ES cell lines provide a valuable model for studying epigenetic regulation in mammalian development.展开更多
Neodymium ion imprinted polymer（Nd^3＋-IIP） particles for selective solid-phase extraction of Nd^3＋ were prepared and determined with inductively coupled plasma atomic emission spectrometry（ICP-AES）.The unleached...Neodymium ion imprinted polymer（Nd^3＋-IIP） particles for selective solid-phase extraction of Nd^3＋ were prepared and determined with inductively coupled plasma atomic emission spectrometry（ICP-AES）.The unleached Nd^3＋-IIP particles were prepared by the copoly-merization of Nd^3＋-5,7-dichloroquinoline-8-ol-4-vinylpyridine ternary complex with styrene and divinyl benzene, then Nd^3＋ was leached to obtain Nd^3＋-IIP particles.The adsorption capacity of the Nd3＋-IIP was 35.18 mg/g.The largest selectivity coefficient for Nd3＋ in the presence of competitive ions such as La^3＋, Ce^3＋, Pr^3＋ and Sm^3＋ was over 110.The proposed method was validated by analyzing two certified reference materials（GBW07301a sediment and GBW07401 soil） and the determined values were in a good agreement with standard values.The method was convenient, selective, sensitive and applicable to the determination of trace Nd^3＋ in environmental samples with complicated matrix.展开更多
Molecularly imprinted polymers （MIPs）, using （S）-naproxen as template and the combination of butyl methacrylate （BMA） and MAA （1：1 molar ratio） as functional monomers were synthesized by an in situ polymeriza...Molecularly imprinted polymers （MIPs）, using （S）-naproxen as template and the combination of butyl methacrylate （BMA） and MAA （1：1 molar ratio） as functional monomers were synthesized by an in situ polymerization reaction. The rendered monolithic column was evaluated in HPLC mode. The result showed that the monolithic MIPs with the combination of two monomers produced better chiral resolution of rac-naproxen （Rs = 1.55） and column efficiencies of imprinted molecules （N = 2860 plates/m） than that with pure MAA.展开更多
A simple and effective method for surface molecularly imprinted composite membranes （MICMs） for artemisinin （Ars） based on regenerated cellulose membranes was first prepared through surface- initiated atom transfe...A simple and effective method for surface molecularly imprinted composite membranes （MICMs） for artemisinin （Ars） based on regenerated cellulose membranes was first prepared through surface- initiated atom transfer radical polymerization （ATRP）, and the as-prepared MICMs were then evaluated as adsorbents for selective recognition and separation of Ars molecules. Batch rebinding studies were conducted to determine the specific adsorption equilibrium, kinetics and selective permeation performance. The adsorption capacity of MICMs toward Ars by the Langmuir isotherm model was 2.008 mgg-1, which was nearly 5.0 times higher than non-molecularly imprinted composite membranes （NICMs）. The kinetic property of MICMs was well-fitted by the pseudo-second-order rate equation. The selective permeation experiments were successfully investigated to prove the excellent selective permeation performance for Ars than the competitive analog （artemether）.展开更多
Synthetic materials that can specifically recognize proteins will find wide application in many fields.In this report,bovine serum albumin was chosen as the template protein.Acrylamide and N,N＇-methylenebisacrylamide...Synthetic materials that can specifically recognize proteins will find wide application in many fields.In this report,bovine serum albumin was chosen as the template protein.Acrylamide and N,N＇-methylenebisacrylamide were employed as the functional and cross-linker monomers,respectively.Molecularly imprinted macroporous monolithic materials that can preferentially bind the template protein in an aqueous environment were prepared by combination of molecular imprinting technique and freezing/thawing preparation method.The resulted imprinted macroporous monolithic columns were evaluated by utilizing as stationary phase in high performance liquid chromatography and solid-phase extraction materials.The experimental results indicated that the imprinted macroporous monolithic column exhibited good recognition for template protein,as compared with the control protein（hemoglobin）,whereas the non-imprinted polymer（prepared under the same conditions except without addition template protein） had no selective properties.展开更多
Chinese herbal medicines(CHMs)play an increasingly important role in the field of medicine and affects public health in the world.Although more and more strict has been employed to ensure the quality and safety of CHM...Chinese herbal medicines(CHMs)play an increasingly important role in the field of medicine and affects public health in the world.Although more and more strict has been employed to ensure the quality and safety of CHMs,pesticide residues in CHMs remain a serious issue and are the bottleneck for the global development of CHMs.In this work,we applied molecularly imprinted membrane electrospray mass spectrometry(MIM-ESI MS)for rapid detecting 4 classes of pesticide residues in CHMs,including organophosphorus(OPP),carbamates,pyrethroids and neonicotinoids in CHMs.Compared with our previous ambient ionization method MESI,MIM-ESI is capable of achieving a~50-fold increase in the detection limit of conventional analytical methods owing to the specificity recognition and unique enrichment of MIM.The optimal experimental conditions were determined,and the method was further validated for its sensitivity and specificity.Our data showed that MIM-ESI MS is applicable for the direct quantitation of pesticide residues in CHMs.This detection technology may help to ensure the quality of CHMs in the future.展开更多
基金supported by the National Natural Science Foundation of China(Nos.32301259,32101228,32271527 and 32371536)the“Pioneer”and“Leading Goose”R&D Program of Zhejiang(Nos.2022C02023 and 2023C02015)+1 种基金the Research Foundation of Talented Scholars of Zhejiang A&F University(No.2021LFR058)the Dean-ship of Scientific Research at Northern Border University,Arar,KSA for funding this research work through the project number“NBU-FPEJ-2024-177-01”.
文摘As a typical bioflavonoid,diosmetin is desirable in the field of natural medicine,healthy food,and cosmetics by anti-cancer,antibacterial,antioxidant,estrogen-like and anti-inflammatory activities,and it comes from a wide range of sources in traditional Chinese medicine like spider fragrance,spearmint and chrysanthemum,as well as in Citrus fruit.However,traditional analytical methods such as silica gel column chromatography face multiple challenges in the selective extraction of diosmetin from biological materials and traditional Chinese medicinal materials.Therefore,it is urgent to develop a new type of absorbent with high efficiency,recyclability and good specificity to diosmetin.In this investigation,a magnetic surface molecularly imprinted polymer(labeled as Diosmetin/SMIPs)was synthesized employing magnetic nanoparticles as the carrier and 4-vinylpyridinyl(4-VP)as the functional monomer by surface imprinting technology.The functional monomer was screened by the binding energy(△E)between functional monomers and template molecules via computational simulation.The Diosmetin/SMIPs had a high level of specific recognition and adsorption capability towards diosmetin with a 20.25 mg g^(-1) adsorption capacity and an imprinting factor(IF)of 2.28.Additionally,it demonstrated excellent regeneration performance with 8 adsorption/desorption cycles.In addition,91.20%-94.16% of spiked diosmetin was recovered from the lemon peel samples.The strategy of constructing Diosmetin/SMIPs based on computational simulation can effectively enhance the specific adsorption performance of diosmetin.Meanwhile,Diosmetin/SMIPs synthesized by imprinting polymerization showed excellent anti-interference and reusability,and realized efficient targeted extraction of diosmetin from lemon peel samples.The results of this investigation provide a promising adsorbent for selective enrichment of diosmetin from Citrus fruit and complicated materials.
文摘In this work,by combining the specific selectivity of molecularly imprinted polymer(MIP)with a simple and sensitive electrochemiluminescence(ECL)detection method,a molecularly imprinted ECL sensor for kanamycin(KA)was developed.The MIP film was synthesized on the surface of gold electrode via electrochemical polymerization,using pyrrole(PY)as the functional monomer and KA as the template molecule.The commonly used luminescent reagent Ru(bpy)32+was employed as the ECL probe,and its co-reagent tripropylamine(TPA)was added to enhance the detection sensitivity of the sensor.Quantitative analysis of KA was performed by evaluating the difference in ECL responses between MIP film electrodes after KA removal and KA rebinding.The sensor exhibited high selectivity,good reproducibility,and stability toward KA,with a linear range of 5.00×10^(-8) to 1.00×10^(-5) mol·L^(-1) and a limit of detection of 1.67×10^(-8) mol·L^(-1)(S/N=3).This work would provide a new idea for the development of simple and sensitive molecularly imprinted ECL sensors.
基金supported by Open Project of State Key Laboratory of Supramolecular Structure and Materials,Jilin University,China(No.sklssm2024018)。
文摘Molecularly imprinted polymers(MIPs)are a kind of synthetic receptors possessing wide application prospects in proteins recognition.However,there are still great challenges in proteins imprinting due to their large size and easy conformation change.In this study,we explored epitope-oriented MIP based on host-vip interaction(hg-MIP)and constructed a novel hg-MIP-SERS(surface-enhanced Raman scatting)approach for efficiently recognizing the terminal epitopes of neuron-specific enolase(NSE),a well-known disease biomarker for small cell lung cancer,neuroblstom,and Alzheimer's disease.The C-and N-terminal epitopes of NSE were modified with 4-(phenylazo)benzoic acid,then they were used as the templates and immobilized onβ-cyclodextrin-functionalized substrates.The imprinted layer was formed by polymerization of various functional monomers.Combined with SERS detection,an antibody-free sandwich assay based on hg-MIP was successfully used to detect the concentration of NSE in human serums,with the advantages of simple operation,small sample volume(5μL),wide linear range(1–10^(4)ng/m L)and a limit of detection as low as 0.01 ng/m L.The developed epitope-oriented hg-MIP-SERS approach can also be extended to other proteins,expanding the imprinting method of proteins,and has a broad development space in the field of protein separation and detection.
文摘Bromocresol green(BCG)and malachite green(MG)are water-soluble toxic organic dyes with adverse health and environmental implications.This study presented a conjugate imprinted adsorbent(CIA)synthesized by incorporating trimethoprim vanillin ligand into a highly crosslinked polymer,designed for the efficient removal of BCG and MG from wastewater.Characterization of CIA involved X-ray powder diffraction,Fourier transform infrared,and scanning electron microscopic analyses.Batch adsorption processes were conducted to evaluate the adsorption characteristics of CIA,with focuses on the effects of contact time,initial dye concentration,pH,and temperature.The molecularly imprinted polymers(MIPs)achieved removal efficiencies of 99.27%and 98.99%at equilibrium for BCG and MG adsorption,respectively.The non-imprinted polymers(NIPs)demonstrated BCG and MG adsorption efficiencies of 51.52%and 62.90%at equilibrium,respectively.Kinetic and isotherm models were employed to elucidate the BCG and MG adsorption mechanisms.The thermodynamic results indicated non-spontaneous and spontaneous reactions for BCG and MG adsorption on MIPs under the examined temperature conditions.The adsorbent exhibited sustained high removal efficiency through five reuse cycles,with no apparent reduction in adsorption performance.Validation of the adsorbent using real textile wastewater samples achieved BCG and MG removal efficiencies of 85.5%-87.5%.The adsorbent outperformed previously reported materials in BCG and MG adsorption.The synthesized CIA is a promising adsorbent for BCG and MG dye removal,contributing to water sustainability.
基金supported by the Project of Key R&D Program of Shandong Province(2023CXGC010712).Geoffrey I.N.
文摘In this work,a novel electrochemical sensor based on covalent organic framework@carbon black@molecularly imprinted polydopamine(COF@CB@MPDA)was developed for selective recognition and determination of ciprofloxacin(CF).COF@CB@MPDA possessed good water dispersibility and was synthesized by the selfpolymerization of dopamine under alkaline conditions in the presence of the COF,CB and CF.The high surface area COF enhanced the adsorption of CF,whilst CB gave the composites high electrical conductivity to improve the sensitivity of the proposed COF@CB@MPDA/glassy carbon electrode(GCE)sensor.The specific recognition of CF by COF@CB@MPDA involved hydrogen bonding and van der Waals interactions.Under optimized conditions,the sensor showed a good linear relationship with CF concentration over the range of 5.0×10^(–7)and 1.0×10^(–4)mol/L,with a limit of detection(LOD)of 9.53×10^(–8)mol/L.Further,the developed sensor exhibited high selectivity,repeatability and stability for CF detection in milk and milk powders.The method used to fabricate the COF@CB@MPDA/GCE sensor could be easily adapted for the selective recognition and detection of other antibacterial agents and organic pollutants in the environment.
基金supported by STI2030-Major Projects(2023ZD04069,Z231100003723004)the National Science Fund for Distinguished Young Scholars(32425041)the Chinese Universities Scientific Fund(2024TC165).
文摘Maize(Zea mays L.)is a globally significant crop essential for food,feed,and bioenergy production.The maize kernel,serving as a primary sink for starch,proteins,lipids,and essential micronutrients,is crucial for enhancing maize yield and quality.Previous studies have established the critical role of Polycomb Repressive Complex 2(PRC2)in regulating kernel development.In this study,we applied a reverse genetics approach to investigate the role of ZmFIE1,the homolog of the PRC2 complex component Extra sex combs(Esc),in maize development.The functional loss of ZmFIE1 significantly reduces embryo size in the early stage but has a relatively small impact on mature kernels.Integrating transcriptional and metabolomic profiling suggests that ZmFIE1 is involved in regulating nutrient balance between the endosperm and embryo.In addition,we demonstrate that ZmFIE1 is maternally expressed,and that the maternal inheritance of the fie1 allele significantly affects the imprinting status of paternally imprinted genes.Overall,our results suggest that ZmFIE1 is a key gene involved in the modulation of embryo development via regulating genomic imprinting and nutrient balance between embryo and endosperm,which provides new insights into the regulation mechanism underlying kernel development.
基金financial support from the National Natural Science Foundation of China(No.22275148)National Key R&D Program of China(No.2018YFB1900201)for Qiuyu Zhang+2 种基金the National Natural Science Foundation of China(No.22271232)Fundamental Research Funds for the Central Universities(No.D5000230114)for Shixin Fathe Fundamental Research Funds for the Central Universities(No.D5000220339)for Qing Liu。
文摘This work develops a protein imprinted nanosphere with varied recognition specificity for bovine serum albumin(BSA)and lysozyme(Lyz)under different UV light through a gradient dual crosslinked imprinting strategy(i.e.,covalent crosslinking and dynamic reversible crosslinking).The imprinting cavities are initially constructed using irreversible covalent crosslinking to specifically recognize BSA,and then the coumarin residues in the imprinting cavities are crosslinked under 365 nm UV light to further imprint Lyz,because Lyz has smaller size than BSA.Since the photo-crosslinking of coumarin is a reversible reaction,the imprinting cavities of Lyz can be de-crosslinked under 254 nm UV light and restore the imprinting cavities of BSA.Moreover,the N-isopropyl acrylamide(NIPAM)and pyrrolidine residues copolymerized in the polymeric surface of the nanospheres are temperature-and p H-responsive respectively.Therefore,the protein rebinding and release behaviors of the nanospheres are controlled by external temperature and p H.As a result,the materials can selectively separate BSA from real bovine whole blood and Lyz from egg white under different UV light.This study may provide a new strategy for construction of protein imprinted materials with tunable specificity for different proteins.
文摘The characterization of these molecularly imprinted polymers is essential to understanding their binding dynamics and structural properties. Through the analysis of the current research, it is found that there are overlaps in the methods used by scholars. The Langmuir equation is frequently applied to model the adsorption isotherms of MIPs, providing critical insight into the capacity and affinity of the binding sites. Infrared Spectroscopy (IR) plays a crucial role in identifying the functional groups involved in the imprinting process and confirming the successful formation of specific binding sites. UV-visible spectrophotometry is employed to monitor the absorption characteristics of the polymers, offering data on the interactions between the template molecules and the polymer matrix. Transmission Electron Microscopy (TEM) provides detailed visualization of the internal structure of MIPs at the nanoscale, revealing the morphology and size of the imprinted cavities. Thermogravimetric Analysis (TGA) assesses the thermal stability and composition of the polymers, identifying decomposition patterns that are indicative of the material’s robustness under different conditions. Finally, the Laser Particle Size Analyzer is used to measure the size distribution of the polymer particles, which is critical for determining the uniformity and efficiency of the imprinting process. The six characterization methods discussed in this paper provide a comprehensive understanding of MIP, and it is hoped that in the future, more optimized design solutions will emerge and their applications in various fields will be enhanced.
基金supported by the National Natural Science Foundation of China(No.50978138)PCSIRT(No.IRT1257),NFFTBS(No.J1103306)the Fundamental Research Funds for the Central Universities for financial support.
文摘There is an urgent imperative to discover practical and efficacious artificial catalysts for the expeditious decontamination of toxic organophosphates. Herein, we propose a novel molecularly imprinted approach utilizing electrospun fiber scaffolds. Specifically, an amidoxime-based functional polymer (PMAOX) has been synthesized, which contains amidoxime groups that can act as nucleophiles and ligands for the formation of catalytic active sites. This polymer was blended with polyacrylonitrile (PAN), a well-processable material, to prepare a nanofiber mat using electrospinning techniques. Then, the amidoxime side groups on fiber surface were further cross-linked after the molecular coordination of templates to complete the molecularly imprinting process. This approach can not only enhance the content of functional molecularly imprinted polymers without affecting structural stability, but also combines surface MI technology to fully expose and leverage the advantages of active sites. The as prepared molecularly imprinted electrospun nanofibers MIF-Zn-PMAOX/PAN-6/4 catalyzes the degradation of paraoxon with a half-life of 32 min, and MIF-Ag-PMAOX/PAN catalyzes the degradation of parathion with a half-life of 18 min. The maximum catalytic rate evidence rate enhancements nearly 3700-fold of the self-hydrolysis. Thus, the mono-amidoxime based molecularly imprinted fibers demonstrate the versatility and superiority as self-detoxifying for organophosphates.
基金supported by the National Natural Science Foundation of China(31570322)。
文摘The early development of the endosperm is crucial for balancing the allocation of maternal nutrients to offspring.This process is believed to be evolutionarily associated with genomic imprinting,resulting in parentally biased allelic gene expression.Beyond Fertilization Independent Seed(FIS)genes,the number of imprinted genes involved in early endosperm development and seed size determination remains limited.This study introduces early endosperm-expressed HAIKU(IKU)downstream Candidate F-box 1(ICF1)and ICF2 as maternally expressed imprinted genes(MEGs)in Arabidopsis thaliana.Although these genes are also demethylated by DEMETER(DME)in the central cell,their activation differs from the direct DME-mediated activation seen in classical MEGs such as the FIS genes.Instead,ICF maternal alleles carry pre-established hypomethylation in their promoters,priming them for activation by the WRKY10 transcription factor in the endosperm.On the contrary,paternal alleles are predominantly suppressed by CG methylation.Furthermore,we find that ICF genes partially contribute to the small seed size observed in iku mutants.Our discovery reveals a two-step regulatory mechanism that highlights the important role of conventional transcription factors in the activation of imprinted genes,which was previously not fully recognized.Therefore,the mechanism provides a new dimension to understand the transcriptional regulation of imprinting in plant reproduction and development.
基金Project supported by the Department of Science and Technology of Zhejiang Province(No.2013C02022-2/01),China
文摘A pre-treatment methodology for clenbuterol hydrochloride （CLEN） isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers （MIPs）. CLEN-imprinted pol- ymers were synthesized by the combined use of ally-β-cyclodextrin （ally-13-CD） and methacrylic acid （MAA）, allyl-β-CD and acrylonitrile （AN）, and allyl-β-CD and methyl methacrylate （MMA） as the binary functional monomers. MAA-linked allyl-β-CD MIPs （M-MAA） were characterized by Fourier transform-infrared （FT-IR） spectroscopy and a scanning electron microscope （SEM）. Based upon the results, M-MAA polymers generally proved to be an excellent selective extraction compared to its references： AN-linked allyl-β-CD MIPs （M-AN） and MMA-linked allyl-β-CD MIPs （M-MMA）. M-MAA polymers were eventually chosen to run through a molecularly imprinted solid-phase extraction （MISPE） micro-column to enrich CLEN residues spiked in pig livers. A high recovery was achieved, ranging from 91.03% to 96.76% with relative standard deviation （RSD） ≤4.45%.
文摘Heavy metal ion is one of the major environmental pollutants.In this study,a Cu(Ⅱ)ions imprinted magnetic chitosan beads are prepared to use chitosan as functional monomer,Cu(Ⅱ)ions as template,Fe_(3)O_(4) as magnetic core and epichlorohydrin and glutaraldehyde as crosslinker,which can be used for removal Cu(Ⅱ)ions from wastewater.The kinetic study shows that the adsorption process follows the pseudosecond-order kinetic equations.The adsorption isotherm study shows that the Langmuir isotherm equation best fits for the monolayer adsorption processes.The selective adsorption properties are performed in Cu(Ⅱ)/Zn(Ⅱ),Cu(Ⅱ)/Ni(Ⅱ),and Cu(Ⅱ)/Co(Ⅱ)binary systems.The results shows that the ⅡMCD has a high selectivity for Cu(Ⅱ)ions in binary systems.The mechanism of ⅡMCD recognition Cu(Ⅱ)ions is also discussed.The results show that the ⅡMCD adsorption Cu(Ⅱ)ions is an enthalpy controlled process.The absolute value of DH(Cu(Ⅱ))and DS(Cu(Ⅱ))is greater than DH(Zn(Ⅱ),Ni(Ⅱ),Co(Ⅱ))and DS(Zn(Ⅱ),Ni(Ⅱ),Co(Ⅱ)),respectively,this indicates that the Cu(Ⅱ)ions have a good spatial matching with imprinted holes on ⅡMCD.The FTIR and XPS also demonstrates the strongly combination of function groups on imprinted holes in the suitable space position.Finally,the ⅡMCD can be regenerated and reused for 10 times without a significantly decreasing in adsorption capacity.This information can be used for further application in the selective removal of Cu(Ⅱ)ions from industrial wastewater.
文摘Dummy molecularly imprinted polymers （DMIPs） for 4-（methylnitrosamino）-1-（3-pyridyl）-1-butanol （NNAL） were produced using three structural analogues as dummy template molecules. The chosen analogues were 4-（acetymethylamino）-1-（3-pyridyl）-butanol, 4- （methylamino）-1-（3-pyridyl）-1-butanol, and 1-（3-pyridyl）-1,4,-butanediol. The molecular recognition characteristics of the produced polymers were evaluated by X-ray photoelec- tron spectroscopy （XPS） and Fourier transform infrared spectroscopy （FT-IR）. Interactions between NNAL and methacrylic acid should be cooperative hydrogen bonds while the ni- trogen atom of the pyridine ring and the oxygen atom of the nitroso group in NNAL are two of the hydrogen-bond acceptors. It was further demonstrated that DMIP synthesized by 4-（acetymethylamino）-1-（3-pyridyl）-butanol had the best binding performance by XPS and FT-IR. Then dummy molecularly imprinted solid phase extraction （DMISPE） was developed for the determination of the analyte using the hit polymer as the sorbing material. Under optimal conditions, the recovery of NNAL dissolved in standard solution reached 93%. And the investigated polymer exhibited much higher binding of NNAL when nicotine was acted as the competitive molecule. Also the proposed method was applied to the measurement of NNAL spiked in blank urine samples with recoveries ranging from 87.2% to 101.2%.
基金This work was supported by the National Natural Science Foundation of China （No.20405013）.
文摘A new bovine serum albumin （BSA） surface imprinting method was developed by the incorporation of quantum dots （QDs） into molecularly imprinted polymers （MIP）, which can offer shape selectivity. Preparation and adsorption conditions were optimized. Physical appearance of the QDs and QDs-MIP particles was illustrated by scanning electron microscope images. Photoluminescence emission of CdS was quenched when rebinding of the template.The quenching of photoluminescence emissions is presumably due to the fluorescence resonance energy transfer between quantum dots and BSA template molecules. The adsorption is compiled with Langmuir isotherm, and chemical adsorption is the rate-controlling step.The maximum adsorption capacity could reach 226.0 mg/g, which is 142.4 mg/g larger than that of undoped BSA MIP. This study demonstrates the validity of QDs coupled with MIP technology for analyzing BSA.
文摘Parthenogenetic embryonic stem （pES） cells provide a valuable in vitro model system for studying the molecular mechanisms that underlie genomic imprinting. However, the pluripotency of pES cells and the expression profiles of paternally expressed imprinted genes have not been fully explored. In this study, three mouse pES cell lines were established and the differentiation potential of these cells in extended culture was evaluated. The undifferentiated cells had a normal karyotype and homozygous genome, and expressed ES-cell-specific molecular markers. The cells remained undifferentiated after more than 50 passages and exhibited pluripotent differentiation capacity. All three lines of the established ES cells produced teratomas; two lines of ES cells produced chimeras and germline transmission. Furthermore, activation of the paternally expressed imprinted genes Snrpn, U2afl-rsl, Peg3, Impact, Zfp127, Dlkl and Mest in these cells was detected. Some paternally expressed imprinted genes were found to be expressed in the blastocyst stage of parthenogenetically activated embryos in vitro and their expression level increased with extended pES cell culture. Furthermore, our data show that the activation of these paternally expressed imprinted genes in pES cells was associated with a change in the methylation of the related differentially methylated regions. These findings provide direct evidence for the pluripotency of pES cells and demonstrate the association between the DNA methylation pattern and the activa- tion of paternally expressed imprinted genes in pES cells. Thus, the established ES cell lines provide a valuable model for studying epigenetic regulation in mammalian development.
文摘Neodymium ion imprinted polymer（Nd^3＋-IIP） particles for selective solid-phase extraction of Nd^3＋ were prepared and determined with inductively coupled plasma atomic emission spectrometry（ICP-AES）.The unleached Nd^3＋-IIP particles were prepared by the copoly-merization of Nd^3＋-5,7-dichloroquinoline-8-ol-4-vinylpyridine ternary complex with styrene and divinyl benzene, then Nd^3＋ was leached to obtain Nd^3＋-IIP particles.The adsorption capacity of the Nd3＋-IIP was 35.18 mg/g.The largest selectivity coefficient for Nd3＋ in the presence of competitive ions such as La^3＋, Ce^3＋, Pr^3＋ and Sm^3＋ was over 110.The proposed method was validated by analyzing two certified reference materials（GBW07301a sediment and GBW07401 soil） and the determined values were in a good agreement with standard values.The method was convenient, selective, sensitive and applicable to the determination of trace Nd^3＋ in environmental samples with complicated matrix.
基金This work was supported by the National Natural Science Foundation of China(20575045)outstanding scholar programs of Tianjin Medical University(to Z.S.Liu).
文摘Molecularly imprinted polymers （MIPs）, using （S）-naproxen as template and the combination of butyl methacrylate （BMA） and MAA （1：1 molar ratio） as functional monomers were synthesized by an in situ polymerization reaction. The rendered monolithic column was evaluated in HPLC mode. The result showed that the monolithic MIPs with the combination of two monomers produced better chiral resolution of rac-naproxen （Rs = 1.55） and column efficiencies of imprinted molecules （N = 2860 plates/m） than that with pure MAA.
基金financially supported by the National Natural Science Foundation of China(Nos.21077046,21107037,21176107,21174057,2100403,21207051)National key basic research development program(973 Program,No. 2012CBB21500)+2 种基金Ph.D.Programs Foundation of Ministry of Education of China(No.20123227120015)Natural Science Foundation of Jiangsu Province(Nos.BK2011461,SBK2011459, BK2011514)China Postdoctoral Science Foundation funded project(Nos.2012M511220,2013M530240)
文摘A simple and effective method for surface molecularly imprinted composite membranes （MICMs） for artemisinin （Ars） based on regenerated cellulose membranes was first prepared through surface- initiated atom transfer radical polymerization （ATRP）, and the as-prepared MICMs were then evaluated as adsorbents for selective recognition and separation of Ars molecules. Batch rebinding studies were conducted to determine the specific adsorption equilibrium, kinetics and selective permeation performance. The adsorption capacity of MICMs toward Ars by the Langmuir isotherm model was 2.008 mgg-1, which was nearly 5.0 times higher than non-molecularly imprinted composite membranes （NICMs）. The kinetic property of MICMs was well-fitted by the pseudo-second-order rate equation. The selective permeation experiments were successfully investigated to prove the excellent selective permeation performance for Ars than the competitive analog （artemether）.
基金supported by the National Natural Science Foundation of China(Nos21075089 and 20605024)Talents Fund of Tianjin University of Science and Technology(No20090424)
文摘Synthetic materials that can specifically recognize proteins will find wide application in many fields.In this report,bovine serum albumin was chosen as the template protein.Acrylamide and N,N＇-methylenebisacrylamide were employed as the functional and cross-linker monomers,respectively.Molecularly imprinted macroporous monolithic materials that can preferentially bind the template protein in an aqueous environment were prepared by combination of molecular imprinting technique and freezing/thawing preparation method.The resulted imprinted macroporous monolithic columns were evaluated by utilizing as stationary phase in high performance liquid chromatography and solid-phase extraction materials.The experimental results indicated that the imprinted macroporous monolithic column exhibited good recognition for template protein,as compared with the control protein（hemoglobin）,whereas the non-imprinted polymer（prepared under the same conditions except without addition template protein） had no selective properties.
基金supported by the National Natural Science Foundation of China(No.82072247)to MZthe Young Scholar Project of Beijing University of Chinese Medicine(Nos.2019-JYBJS-017 and 2021-JYB-XJSJJ001)to SJ and MZ。
文摘Chinese herbal medicines(CHMs)play an increasingly important role in the field of medicine and affects public health in the world.Although more and more strict has been employed to ensure the quality and safety of CHMs,pesticide residues in CHMs remain a serious issue and are the bottleneck for the global development of CHMs.In this work,we applied molecularly imprinted membrane electrospray mass spectrometry(MIM-ESI MS)for rapid detecting 4 classes of pesticide residues in CHMs,including organophosphorus(OPP),carbamates,pyrethroids and neonicotinoids in CHMs.Compared with our previous ambient ionization method MESI,MIM-ESI is capable of achieving a~50-fold increase in the detection limit of conventional analytical methods owing to the specificity recognition and unique enrichment of MIM.The optimal experimental conditions were determined,and the method was further validated for its sensitivity and specificity.Our data showed that MIM-ESI MS is applicable for the direct quantitation of pesticide residues in CHMs.This detection technology may help to ensure the quality of CHMs in the future.
