Molecularly imprinted polymers(MIPs)are a kind of synthetic receptors possessing wide application prospects in proteins recognition.However,there are still great challenges in proteins imprinting due to their large si...Molecularly imprinted polymers(MIPs)are a kind of synthetic receptors possessing wide application prospects in proteins recognition.However,there are still great challenges in proteins imprinting due to their large size and easy conformation change.In this study,we explored epitope-oriented MIP based on host-vip interaction(hg-MIP)and constructed a novel hg-MIP-SERS(surface-enhanced Raman scatting)approach for efficiently recognizing the terminal epitopes of neuron-specific enolase(NSE),a well-known disease biomarker for small cell lung cancer,neuroblstom,and Alzheimer's disease.The C-and N-terminal epitopes of NSE were modified with 4-(phenylazo)benzoic acid,then they were used as the templates and immobilized onβ-cyclodextrin-functionalized substrates.The imprinted layer was formed by polymerization of various functional monomers.Combined with SERS detection,an antibody-free sandwich assay based on hg-MIP was successfully used to detect the concentration of NSE in human serums,with the advantages of simple operation,small sample volume(5μL),wide linear range(1–10^(4)ng/m L)and a limit of detection as low as 0.01 ng/m L.The developed epitope-oriented hg-MIP-SERS approach can also be extended to other proteins,expanding the imprinting method of proteins,and has a broad development space in the field of protein separation and detection.展开更多
As a typical bioflavonoid,diosmetin is desirable in the field of natural medicine,healthy food,and cosmetics by anti-cancer,antibacterial,antioxidant,estrogen-like and anti-inflammatory activities,and it comes from a ...As a typical bioflavonoid,diosmetin is desirable in the field of natural medicine,healthy food,and cosmetics by anti-cancer,antibacterial,antioxidant,estrogen-like and anti-inflammatory activities,and it comes from a wide range of sources in traditional Chinese medicine like spider fragrance,spearmint and chrysanthemum,as well as in Citrus fruit.However,traditional analytical methods such as silica gel column chromatography face multiple challenges in the selective extraction of diosmetin from biological materials and traditional Chinese medicinal materials.Therefore,it is urgent to develop a new type of absorbent with high efficiency,recyclability and good specificity to diosmetin.In this investigation,a magnetic surface molecularly imprinted polymer(labeled as Diosmetin/SMIPs)was synthesized employing magnetic nanoparticles as the carrier and 4-vinylpyridinyl(4-VP)as the functional monomer by surface imprinting technology.The functional monomer was screened by the binding energy(△E)between functional monomers and template molecules via computational simulation.The Diosmetin/SMIPs had a high level of specific recognition and adsorption capability towards diosmetin with a 20.25 mg g^(-1) adsorption capacity and an imprinting factor(IF)of 2.28.Additionally,it demonstrated excellent regeneration performance with 8 adsorption/desorption cycles.In addition,91.20%-94.16% of spiked diosmetin was recovered from the lemon peel samples.The strategy of constructing Diosmetin/SMIPs based on computational simulation can effectively enhance the specific adsorption performance of diosmetin.Meanwhile,Diosmetin/SMIPs synthesized by imprinting polymerization showed excellent anti-interference and reusability,and realized efficient targeted extraction of diosmetin from lemon peel samples.The results of this investigation provide a promising adsorbent for selective enrichment of diosmetin from Citrus fruit and complicated materials.展开更多
In this work,by combining the specific selectivity of molecularly imprinted polymer(MIP)with a simple and sensitive electrochemiluminescence(ECL)detection method,a molecularly imprinted ECL sensor for kanamycin(KA)was...In this work,by combining the specific selectivity of molecularly imprinted polymer(MIP)with a simple and sensitive electrochemiluminescence(ECL)detection method,a molecularly imprinted ECL sensor for kanamycin(KA)was developed.The MIP film was synthesized on the surface of gold electrode via electrochemical polymerization,using pyrrole(PY)as the functional monomer and KA as the template molecule.The commonly used luminescent reagent Ru(bpy)32+was employed as the ECL probe,and its co-reagent tripropylamine(TPA)was added to enhance the detection sensitivity of the sensor.Quantitative analysis of KA was performed by evaluating the difference in ECL responses between MIP film electrodes after KA removal and KA rebinding.The sensor exhibited high selectivity,good reproducibility,and stability toward KA,with a linear range of 5.00×10^(-8) to 1.00×10^(-5) mol·L^(-1) and a limit of detection of 1.67×10^(-8) mol·L^(-1)(S/N=3).This work would provide a new idea for the development of simple and sensitive molecularly imprinted ECL sensors.展开更多
Bromocresol green(BCG)and malachite green(MG)are water-soluble toxic organic dyes with adverse health and environmental implications.This study presented a conjugate imprinted adsorbent(CIA)synthesized by incorporatin...Bromocresol green(BCG)and malachite green(MG)are water-soluble toxic organic dyes with adverse health and environmental implications.This study presented a conjugate imprinted adsorbent(CIA)synthesized by incorporating trimethoprim vanillin ligand into a highly crosslinked polymer,designed for the efficient removal of BCG and MG from wastewater.Characterization of CIA involved X-ray powder diffraction,Fourier transform infrared,and scanning electron microscopic analyses.Batch adsorption processes were conducted to evaluate the adsorption characteristics of CIA,with focuses on the effects of contact time,initial dye concentration,pH,and temperature.The molecularly imprinted polymers(MIPs)achieved removal efficiencies of 99.27%and 98.99%at equilibrium for BCG and MG adsorption,respectively.The non-imprinted polymers(NIPs)demonstrated BCG and MG adsorption efficiencies of 51.52%and 62.90%at equilibrium,respectively.Kinetic and isotherm models were employed to elucidate the BCG and MG adsorption mechanisms.The thermodynamic results indicated non-spontaneous and spontaneous reactions for BCG and MG adsorption on MIPs under the examined temperature conditions.The adsorbent exhibited sustained high removal efficiency through five reuse cycles,with no apparent reduction in adsorption performance.Validation of the adsorbent using real textile wastewater samples achieved BCG and MG removal efficiencies of 85.5%-87.5%.The adsorbent outperformed previously reported materials in BCG and MG adsorption.The synthesized CIA is a promising adsorbent for BCG and MG dye removal,contributing to water sustainability.展开更多
Phase imprinting enables the dynamic generation of superflow in bosonic atoms,effectively overcoming traditional limitations such as vortex number constraints and heating effects.However,the mechanisms underlying supe...Phase imprinting enables the dynamic generation of superflow in bosonic atoms,effectively overcoming traditional limitations such as vortex number constraints and heating effects.However,the mechanisms underlying superflow formation remain insufficiently understood.In this work,we reveal these mechanisms by studying the time evolution of the transferred total angular momentum and the quantized current throughout the phase imprinting process,achieved through numerically solving the time-dependent Schrodinger and Gross-Pitaevskii equations.We demonstrate that the Bose gas dynamically acquires angular momentum through the density depletion induced by the phase imprinting potential,whereas quantized currents emerge from azimuthal phase slips accompanied by complete density depletions.Regarding the impact of system parameters,such as interactions,we find that interactions hinder superflow formation,as the azimuthal density distribution becomes less susceptible to the phase imprinting potential.Our findings offer microscopic insights into the dynamic development of superflow during the phase imprinting process and provide valuable guidance for ongoing experimental efforts.展开更多
This work develops a protein imprinted nanosphere with varied recognition specificity for bovine serum albumin(BSA)and lysozyme(Lyz)under different UV light through a gradient dual crosslinked imprinting strategy(i.e....This work develops a protein imprinted nanosphere with varied recognition specificity for bovine serum albumin(BSA)and lysozyme(Lyz)under different UV light through a gradient dual crosslinked imprinting strategy(i.e.,covalent crosslinking and dynamic reversible crosslinking).The imprinting cavities are initially constructed using irreversible covalent crosslinking to specifically recognize BSA,and then the coumarin residues in the imprinting cavities are crosslinked under 365 nm UV light to further imprint Lyz,because Lyz has smaller size than BSA.Since the photo-crosslinking of coumarin is a reversible reaction,the imprinting cavities of Lyz can be de-crosslinked under 254 nm UV light and restore the imprinting cavities of BSA.Moreover,the N-isopropyl acrylamide(NIPAM)and pyrrolidine residues copolymerized in the polymeric surface of the nanospheres are temperature-and p H-responsive respectively.Therefore,the protein rebinding and release behaviors of the nanospheres are controlled by external temperature and p H.As a result,the materials can selectively separate BSA from real bovine whole blood and Lyz from egg white under different UV light.This study may provide a new strategy for construction of protein imprinted materials with tunable specificity for different proteins.展开更多
In this work,a novel electrochemical sensor based on covalent organic framework@carbon black@molecularly imprinted polydopamine(COF@CB@MPDA)was developed for selective recognition and determination of ciprofloxacin(CF...In this work,a novel electrochemical sensor based on covalent organic framework@carbon black@molecularly imprinted polydopamine(COF@CB@MPDA)was developed for selective recognition and determination of ciprofloxacin(CF).COF@CB@MPDA possessed good water dispersibility and was synthesized by the selfpolymerization of dopamine under alkaline conditions in the presence of the COF,CB and CF.The high surface area COF enhanced the adsorption of CF,whilst CB gave the composites high electrical conductivity to improve the sensitivity of the proposed COF@CB@MPDA/glassy carbon electrode(GCE)sensor.The specific recognition of CF by COF@CB@MPDA involved hydrogen bonding and van der Waals interactions.Under optimized conditions,the sensor showed a good linear relationship with CF concentration over the range of 5.0×10^(–7)and 1.0×10^(–4)mol/L,with a limit of detection(LOD)of 9.53×10^(–8)mol/L.Further,the developed sensor exhibited high selectivity,repeatability and stability for CF detection in milk and milk powders.The method used to fabricate the COF@CB@MPDA/GCE sensor could be easily adapted for the selective recognition and detection of other antibacterial agents and organic pollutants in the environment.展开更多
Maize(Zea mays L.)is a globally significant crop essential for food,feed,and bioenergy production.The maize kernel,serving as a primary sink for starch,proteins,lipids,and essential micronutrients,is crucial for enhan...Maize(Zea mays L.)is a globally significant crop essential for food,feed,and bioenergy production.The maize kernel,serving as a primary sink for starch,proteins,lipids,and essential micronutrients,is crucial for enhancing maize yield and quality.Previous studies have established the critical role of Polycomb Repressive Complex 2(PRC2)in regulating kernel development.In this study,we applied a reverse genetics approach to investigate the role of ZmFIE1,the homolog of the PRC2 complex component Extra sex combs(Esc),in maize development.The functional loss of ZmFIE1 significantly reduces embryo size in the early stage but has a relatively small impact on mature kernels.Integrating transcriptional and metabolomic profiling suggests that ZmFIE1 is involved in regulating nutrient balance between the endosperm and embryo.In addition,we demonstrate that ZmFIE1 is maternally expressed,and that the maternal inheritance of the fie1 allele significantly affects the imprinting status of paternally imprinted genes.Overall,our results suggest that ZmFIE1 is a key gene involved in the modulation of embryo development via regulating genomic imprinting and nutrient balance between embryo and endosperm,which provides new insights into the regulation mechanism underlying kernel development.展开更多
Quantitative determination of tetracycline(TC)in environment and foods is of great importance,as excessive residues might have negative effects on human health and environmental risks.Herein,a selfpowered molecularly ...Quantitative determination of tetracycline(TC)in environment and foods is of great importance,as excessive residues might have negative effects on human health and environmental risks.Herein,a selfpowered molecularly imprinted photoelectrochemical(PEC)sensor based on the Zn O/C photoanode and the Fe-doped CuBi_(2)O_(4)(CBFO)photocathode is developed for the sensitive detection of TC.The photocathodic current can be amplified by the efficient electron transfer caused by the Fermi energy level gap between the photoanode and photocathode.Furthermore,molecularly imprinted polymers(MIPs)at photocathode can selectivity identify the TC templates and thus improve the specificity.Under the optimal conditions,the sensor has a linear range of 10^(-2)-1.0×10^(5) nmol/L,and a limit of detection(LOD)of 0.007 nmol/L(S/N=3).More crucially,the milk sample detection is carried out using the as-prepared sensor,and the outcome is satisfactory.The research gives us a novel sensing platform for quick and accurate antibiotic(like TC)in environment and food monitoring.展开更多
Genomic imprinting is the epigenetic phenomenon by which certain genes are expressed in a parent-of-origin-specific manner, and was first discovered in mammalian embryos. Recent studies have shown that it also occurs ...Genomic imprinting is the epigenetic phenomenon by which certain genes are expressed in a parent-of-origin-specific manner, and was first discovered in mammalian embryos. Recent studies have shown that it also occurs in developing plant seeds, and is now becoming a hot topic of biology of plant seed development. According to the previous studies on imprinted genes, imprinting mechanism and their roles in plant seed development, the current progress of genomic imprinting in plant seed development was summarized and possible strategies were proposed to deal with the problems, which could provide helpful information for further research.展开更多
A novel Ni（Ⅱ） ion-imprinted silica gel polymer was prepared via the surface imprinting technique combined with aqueous solution polymerization by using 2-acrylamido-2-methyl-1-propanesulfonic acid（AMPS） as a func...A novel Ni（Ⅱ） ion-imprinted silica gel polymer was prepared via the surface imprinting technique combined with aqueous solution polymerization by using 2-acrylamido-2-methyl-1-propanesulfonic acid（AMPS） as a functional monomer for the selective separation of Ni（Ⅱ） from aqueous solution. The sorbent showed good chemical and thermal stability. Kinetics studies indicated that the equilibrium adsorption was achieved within 10 min and the adsorption kinetics fitted well with the pseudo-second-order kinetic model. The maximum adsorption capacity of the ion-imprinted polymer towards Ni（Ⅱ） at the optimal p H of 7.0 was 66.22 mg·g^（-1）. The relative selectivity coefficients of the sorbent were 9.23, 15.71, 14.72 and 20.15 for Ni（Ⅱ）/Co（Ⅱ）, Ni（Ⅱ）/Cu（Ⅱ）, Ni（Ⅱ）/Zn（Ⅱ） and Ni（Ⅱ）/Pb（Ⅱ）, respectively. The adsorption isotherm fitted well with Langmuir isotherm model. The thermodynamic results indicated that the adsorption of Ni（Ⅱ） was a spontaneous and endothermic process. The sorbent showed good reusability evidenced by six cycles of adsorption/desorption experiments. The precision of this method is satisfactory. Thus, the prepared sorbent can be considered as a promising sorbent for selective separation of Ni（Ⅱ） in real water samples.展开更多
Epigenetic dysregulation comprising DNA hypermethylation and hypomethylation, enhancer of zeste homologue 2 （EZH2） overexpression and altered patterns of histone modifications is associated with the progression of p...Epigenetic dysregulation comprising DNA hypermethylation and hypomethylation, enhancer of zeste homologue 2 （EZH2） overexpression and altered patterns of histone modifications is associated with the progression of prostate cancer. DNA methylation, EZH2 and histone modifications also ensure the parental-specific monoallelic expression of at least 62 imprinted genes. Although it is therefore tempting to speculate that epigenetic dysregulation may extend to imprinted genes, expression changes in cancerous prostates are only well documented for insulin-like growth factor 2 （IGF2）. A literature and database survey on imprinted genes in prostate cancer suggests that the expression of most imprinted genes remains unchanged despite global disturbances in epigenetic mechanisms. Instead, selective genetic and epigenetic changes appear to lead to the inactivation of a sub-network of imprinted genes, which might function in the prostate to limit cell growth induced via the PI3K/Akt pathway, modulate androgen responses and regulate differentiation. Whereas dysregulation of IGF2 may constitute an early change in prostate carcinogenesis, inactivation of this imprinted gene network is rather associated with cancer progression.展开更多
This study demonstrates the preparation and characterization of a novel ion imprinted cryogel which exhibits high affinity and selectivity towards Ce（Ⅲ） ions in aqueous solutions and bastnasite ore samples.2-Hydrox...This study demonstrates the preparation and characterization of a novel ion imprinted cryogel which exhibits high affinity and selectivity towards Ce（Ⅲ） ions in aqueous solutions and bastnasite ore samples.2-Hydroxyethyl methacrylate（HEMA） and N-methacryloylamido antipyrine（MAAP） were used as functional monomers for the preparation of Ce（Ⅲ） imprinted cryogel. The effects of various factors such as initial Ce（Ⅲ） concentration, flow rate, pH, interaction time and ionic strength on the Ce（Ⅲ） binding to the prepared ion imprinted cryogels were also studied. The binding equilibrium for Ce（Ⅲ） is obtained in30 min at the flow rate of 0.5 mL/min. The maximum binding capacity of the prepared ion imprinted cryogel towards Ce（Ⅲ） is obtained as 36.58 mg/g at optimum conditions. The selectivity of the prepared ion imprinted cryogel towards Ce（Ⅲ） in the presence of other possible interfering lanthanide ions such as La（Ⅲ） and Nd（Ⅲ） were also performed. The obtained results showed that the prepared ion imprinted cryogel exhibits high selectivity and sensitivity towards Ce（Ⅲ） ions. The limit of detection（LOD） was found as 50 μg/L.展开更多
DEAR EDITOR,Genomic imprinting often results in parent-of-origin specific differential expression of maternally and paternally inherited alleles and plays an essential role in mammalian development and growth.Mammalia...DEAR EDITOR,Genomic imprinting often results in parent-of-origin specific differential expression of maternally and paternally inherited alleles and plays an essential role in mammalian development and growth.Mammalian genomic imprinting has primarily been studied in mice and humans,with only limited information available for pigs.To systematically characterize this phenomenon and evaluate imprinting status between different species,we investigated imprinted genes on a genome-wide scale in pig brain tissues.展开更多
Neural stem cells(NSCs) and imprinted genes play an important role in brain development. On historical grounds, these two determinants have been largely studied independently of each other. Recent evidence suggests, h...Neural stem cells(NSCs) and imprinted genes play an important role in brain development. On historical grounds, these two determinants have been largely studied independently of each other. Recent evidence suggests, however, that NSCs can reset select genomic imprints to prevent precocious depletion of the stem cell reservoir. Moreover, imprinted genes like the transcriptional regulator Zac1 can fine tune neuronal vs astroglial differentiation of NSCs. Zac1 binds in a sequence-specific manner to pro-neuronal and imprinted genes to confer transcriptional regulation and furthermore coregulates members of the p53-family in NSCs. At the genome scale, Zac1 is a central hub of an imprinted gene network comprising genes with animportant role for NSC quiescence, proliferation and differentiation. Overall, transcriptional, epigenomic, and genomic mechanisms seem to coordinate the functional relationships of NSCs and imprinted genes from development to maturation, and possibly aging.展开更多
In this work,the quercetin imprinted core-shell microspheres were prepared using silica surface imprinting technique.A simple sol-gel procedure was used for the synthesis of the imprinted materials with 3-aminopropylt...In this work,the quercetin imprinted core-shell microspheres were prepared using silica surface imprinting technique.A simple sol-gel procedure was used for the synthesis of the imprinted materials with 3-aminopropyltriethoxysilane as functional monomer and tetraethyl orthosilicate as crosslinker.The SEM images indicated that the MIPs shell was successfully grafted onto the silica surface.The characteristics of the molecularly imprinted polymers such as capacity,selectivity and absorption dynamic were investigated by rebinding experiments.The results showed that the prepared MIPs had good imprinting effect and adsorption amount of quercetin.展开更多
A pre-treatment methodology for clenbuterol hydrochloride （CLEN） isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers （MIPs）. CLEN-imprinted pol-...A pre-treatment methodology for clenbuterol hydrochloride （CLEN） isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers （MIPs）. CLEN-imprinted pol- ymers were synthesized by the combined use of ally-β-cyclodextrin （ally-13-CD） and methacrylic acid （MAA）, allyl-β-CD and acrylonitrile （AN）, and allyl-β-CD and methyl methacrylate （MMA） as the binary functional monomers. MAA-linked allyl-β-CD MIPs （M-MAA） were characterized by Fourier transform-infrared （FT-IR） spectroscopy and a scanning electron microscope （SEM）. Based upon the results, M-MAA polymers generally proved to be an excellent selective extraction compared to its references： AN-linked allyl-β-CD MIPs （M-AN） and MMA-linked allyl-β-CD MIPs （M-MMA）. M-MAA polymers were eventually chosen to run through a molecularly imprinted solid-phase extraction （MISPE） micro-column to enrich CLEN residues spiked in pig livers. A high recovery was achieved, ranging from 91.03% to 96.76% with relative standard deviation （RSD） ≤4.45%.展开更多
As a novel technique, molecular imprinting technique are attracting more and more attention in recent years, due to its specific recognition function to im- printed molecules. This paper firstly introduced this techni...As a novel technique, molecular imprinting technique are attracting more and more attention in recent years, due to its specific recognition function to im- printed molecules. This paper firstly introduced this technique from its history, princi- ple, classification and methods, and then reviewed the application in food industry, and presented possible future research orientations.展开更多
The uniform surface ion-imprinted resins for Zn2+ as the imprinting vip were prepared by emulsifier-free emulsion polymerization utilizing ally phenyl hydrogenphosphate as a functional comonomer. The Zn2+-imprinted ...The uniform surface ion-imprinted resins for Zn2+ as the imprinting vip were prepared by emulsifier-free emulsion polymerization utilizing ally phenyl hydrogenphosphate as a functional comonomer. The Zn2+-imprinted resin adsorbed Zn2+ much more effectively than did the unimprinted one. The selective feature of the surface imprinted resins to the template ions was demonstrated.展开更多
Heavy metal ion is one of the major environmental pollutants.In this study,a Cu(Ⅱ)ions imprinted magnetic chitosan beads are prepared to use chitosan as functional monomer,Cu(Ⅱ)ions as template,Fe_(3)O_(4) as magnet...Heavy metal ion is one of the major environmental pollutants.In this study,a Cu(Ⅱ)ions imprinted magnetic chitosan beads are prepared to use chitosan as functional monomer,Cu(Ⅱ)ions as template,Fe_(3)O_(4) as magnetic core and epichlorohydrin and glutaraldehyde as crosslinker,which can be used for removal Cu(Ⅱ)ions from wastewater.The kinetic study shows that the adsorption process follows the pseudosecond-order kinetic equations.The adsorption isotherm study shows that the Langmuir isotherm equation best fits for the monolayer adsorption processes.The selective adsorption properties are performed in Cu(Ⅱ)/Zn(Ⅱ),Cu(Ⅱ)/Ni(Ⅱ),and Cu(Ⅱ)/Co(Ⅱ)binary systems.The results shows that the ⅡMCD has a high selectivity for Cu(Ⅱ)ions in binary systems.The mechanism of ⅡMCD recognition Cu(Ⅱ)ions is also discussed.The results show that the ⅡMCD adsorption Cu(Ⅱ)ions is an enthalpy controlled process.The absolute value of DH(Cu(Ⅱ))and DS(Cu(Ⅱ))is greater than DH(Zn(Ⅱ),Ni(Ⅱ),Co(Ⅱ))and DS(Zn(Ⅱ),Ni(Ⅱ),Co(Ⅱ)),respectively,this indicates that the Cu(Ⅱ)ions have a good spatial matching with imprinted holes on ⅡMCD.The FTIR and XPS also demonstrates the strongly combination of function groups on imprinted holes in the suitable space position.Finally,the ⅡMCD can be regenerated and reused for 10 times without a significantly decreasing in adsorption capacity.This information can be used for further application in the selective removal of Cu(Ⅱ)ions from industrial wastewater.展开更多
基金supported by Open Project of State Key Laboratory of Supramolecular Structure and Materials,Jilin University,China(No.sklssm2024018)。
文摘Molecularly imprinted polymers(MIPs)are a kind of synthetic receptors possessing wide application prospects in proteins recognition.However,there are still great challenges in proteins imprinting due to their large size and easy conformation change.In this study,we explored epitope-oriented MIP based on host-vip interaction(hg-MIP)and constructed a novel hg-MIP-SERS(surface-enhanced Raman scatting)approach for efficiently recognizing the terminal epitopes of neuron-specific enolase(NSE),a well-known disease biomarker for small cell lung cancer,neuroblstom,and Alzheimer's disease.The C-and N-terminal epitopes of NSE were modified with 4-(phenylazo)benzoic acid,then they were used as the templates and immobilized onβ-cyclodextrin-functionalized substrates.The imprinted layer was formed by polymerization of various functional monomers.Combined with SERS detection,an antibody-free sandwich assay based on hg-MIP was successfully used to detect the concentration of NSE in human serums,with the advantages of simple operation,small sample volume(5μL),wide linear range(1–10^(4)ng/m L)and a limit of detection as low as 0.01 ng/m L.The developed epitope-oriented hg-MIP-SERS approach can also be extended to other proteins,expanding the imprinting method of proteins,and has a broad development space in the field of protein separation and detection.
基金supported by the National Natural Science Foundation of China(Nos.32301259,32101228,32271527 and 32371536)the“Pioneer”and“Leading Goose”R&D Program of Zhejiang(Nos.2022C02023 and 2023C02015)+1 种基金the Research Foundation of Talented Scholars of Zhejiang A&F University(No.2021LFR058)the Dean-ship of Scientific Research at Northern Border University,Arar,KSA for funding this research work through the project number“NBU-FPEJ-2024-177-01”.
文摘As a typical bioflavonoid,diosmetin is desirable in the field of natural medicine,healthy food,and cosmetics by anti-cancer,antibacterial,antioxidant,estrogen-like and anti-inflammatory activities,and it comes from a wide range of sources in traditional Chinese medicine like spider fragrance,spearmint and chrysanthemum,as well as in Citrus fruit.However,traditional analytical methods such as silica gel column chromatography face multiple challenges in the selective extraction of diosmetin from biological materials and traditional Chinese medicinal materials.Therefore,it is urgent to develop a new type of absorbent with high efficiency,recyclability and good specificity to diosmetin.In this investigation,a magnetic surface molecularly imprinted polymer(labeled as Diosmetin/SMIPs)was synthesized employing magnetic nanoparticles as the carrier and 4-vinylpyridinyl(4-VP)as the functional monomer by surface imprinting technology.The functional monomer was screened by the binding energy(△E)between functional monomers and template molecules via computational simulation.The Diosmetin/SMIPs had a high level of specific recognition and adsorption capability towards diosmetin with a 20.25 mg g^(-1) adsorption capacity and an imprinting factor(IF)of 2.28.Additionally,it demonstrated excellent regeneration performance with 8 adsorption/desorption cycles.In addition,91.20%-94.16% of spiked diosmetin was recovered from the lemon peel samples.The strategy of constructing Diosmetin/SMIPs based on computational simulation can effectively enhance the specific adsorption performance of diosmetin.Meanwhile,Diosmetin/SMIPs synthesized by imprinting polymerization showed excellent anti-interference and reusability,and realized efficient targeted extraction of diosmetin from lemon peel samples.The results of this investigation provide a promising adsorbent for selective enrichment of diosmetin from Citrus fruit and complicated materials.
文摘In this work,by combining the specific selectivity of molecularly imprinted polymer(MIP)with a simple and sensitive electrochemiluminescence(ECL)detection method,a molecularly imprinted ECL sensor for kanamycin(KA)was developed.The MIP film was synthesized on the surface of gold electrode via electrochemical polymerization,using pyrrole(PY)as the functional monomer and KA as the template molecule.The commonly used luminescent reagent Ru(bpy)32+was employed as the ECL probe,and its co-reagent tripropylamine(TPA)was added to enhance the detection sensitivity of the sensor.Quantitative analysis of KA was performed by evaluating the difference in ECL responses between MIP film electrodes after KA removal and KA rebinding.The sensor exhibited high selectivity,good reproducibility,and stability toward KA,with a linear range of 5.00×10^(-8) to 1.00×10^(-5) mol·L^(-1) and a limit of detection of 1.67×10^(-8) mol·L^(-1)(S/N=3).This work would provide a new idea for the development of simple and sensitive molecularly imprinted ECL sensors.
文摘Bromocresol green(BCG)and malachite green(MG)are water-soluble toxic organic dyes with adverse health and environmental implications.This study presented a conjugate imprinted adsorbent(CIA)synthesized by incorporating trimethoprim vanillin ligand into a highly crosslinked polymer,designed for the efficient removal of BCG and MG from wastewater.Characterization of CIA involved X-ray powder diffraction,Fourier transform infrared,and scanning electron microscopic analyses.Batch adsorption processes were conducted to evaluate the adsorption characteristics of CIA,with focuses on the effects of contact time,initial dye concentration,pH,and temperature.The molecularly imprinted polymers(MIPs)achieved removal efficiencies of 99.27%and 98.99%at equilibrium for BCG and MG adsorption,respectively.The non-imprinted polymers(NIPs)demonstrated BCG and MG adsorption efficiencies of 51.52%and 62.90%at equilibrium,respectively.Kinetic and isotherm models were employed to elucidate the BCG and MG adsorption mechanisms.The thermodynamic results indicated non-spontaneous and spontaneous reactions for BCG and MG adsorption on MIPs under the examined temperature conditions.The adsorbent exhibited sustained high removal efficiency through five reuse cycles,with no apparent reduction in adsorption performance.Validation of the adsorbent using real textile wastewater samples achieved BCG and MG removal efficiencies of 85.5%-87.5%.The adsorbent outperformed previously reported materials in BCG and MG adsorption.The synthesized CIA is a promising adsorbent for BCG and MG dye removal,contributing to water sustainability.
基金supported by the National Natural Science Foundation of China(Grants Nos.12104406 and 12204105)the Natural Science Foundation Zhejiang Province,China(Grant No.ZCLMS25A0401)+1 种基金the Startup Grant of Zhejiang SciTech University(Grant No.21062338-Y)the Natural Science Foundation of Fujian Province,China(Grant No.2022J05116)。
文摘Phase imprinting enables the dynamic generation of superflow in bosonic atoms,effectively overcoming traditional limitations such as vortex number constraints and heating effects.However,the mechanisms underlying superflow formation remain insufficiently understood.In this work,we reveal these mechanisms by studying the time evolution of the transferred total angular momentum and the quantized current throughout the phase imprinting process,achieved through numerically solving the time-dependent Schrodinger and Gross-Pitaevskii equations.We demonstrate that the Bose gas dynamically acquires angular momentum through the density depletion induced by the phase imprinting potential,whereas quantized currents emerge from azimuthal phase slips accompanied by complete density depletions.Regarding the impact of system parameters,such as interactions,we find that interactions hinder superflow formation,as the azimuthal density distribution becomes less susceptible to the phase imprinting potential.Our findings offer microscopic insights into the dynamic development of superflow during the phase imprinting process and provide valuable guidance for ongoing experimental efforts.
基金financial support from the National Natural Science Foundation of China(No.22275148)National Key R&D Program of China(No.2018YFB1900201)for Qiuyu Zhang+2 种基金the National Natural Science Foundation of China(No.22271232)Fundamental Research Funds for the Central Universities(No.D5000230114)for Shixin Fathe Fundamental Research Funds for the Central Universities(No.D5000220339)for Qing Liu。
文摘This work develops a protein imprinted nanosphere with varied recognition specificity for bovine serum albumin(BSA)and lysozyme(Lyz)under different UV light through a gradient dual crosslinked imprinting strategy(i.e.,covalent crosslinking and dynamic reversible crosslinking).The imprinting cavities are initially constructed using irreversible covalent crosslinking to specifically recognize BSA,and then the coumarin residues in the imprinting cavities are crosslinked under 365 nm UV light to further imprint Lyz,because Lyz has smaller size than BSA.Since the photo-crosslinking of coumarin is a reversible reaction,the imprinting cavities of Lyz can be de-crosslinked under 254 nm UV light and restore the imprinting cavities of BSA.Moreover,the N-isopropyl acrylamide(NIPAM)and pyrrolidine residues copolymerized in the polymeric surface of the nanospheres are temperature-and p H-responsive respectively.Therefore,the protein rebinding and release behaviors of the nanospheres are controlled by external temperature and p H.As a result,the materials can selectively separate BSA from real bovine whole blood and Lyz from egg white under different UV light.This study may provide a new strategy for construction of protein imprinted materials with tunable specificity for different proteins.
基金supported by the Project of Key R&D Program of Shandong Province(2023CXGC010712).Geoffrey I.N.
文摘In this work,a novel electrochemical sensor based on covalent organic framework@carbon black@molecularly imprinted polydopamine(COF@CB@MPDA)was developed for selective recognition and determination of ciprofloxacin(CF).COF@CB@MPDA possessed good water dispersibility and was synthesized by the selfpolymerization of dopamine under alkaline conditions in the presence of the COF,CB and CF.The high surface area COF enhanced the adsorption of CF,whilst CB gave the composites high electrical conductivity to improve the sensitivity of the proposed COF@CB@MPDA/glassy carbon electrode(GCE)sensor.The specific recognition of CF by COF@CB@MPDA involved hydrogen bonding and van der Waals interactions.Under optimized conditions,the sensor showed a good linear relationship with CF concentration over the range of 5.0×10^(–7)and 1.0×10^(–4)mol/L,with a limit of detection(LOD)of 9.53×10^(–8)mol/L.Further,the developed sensor exhibited high selectivity,repeatability and stability for CF detection in milk and milk powders.The method used to fabricate the COF@CB@MPDA/GCE sensor could be easily adapted for the selective recognition and detection of other antibacterial agents and organic pollutants in the environment.
基金supported by STI2030-Major Projects(2023ZD04069,Z231100003723004)the National Science Fund for Distinguished Young Scholars(32425041)the Chinese Universities Scientific Fund(2024TC165).
文摘Maize(Zea mays L.)is a globally significant crop essential for food,feed,and bioenergy production.The maize kernel,serving as a primary sink for starch,proteins,lipids,and essential micronutrients,is crucial for enhancing maize yield and quality.Previous studies have established the critical role of Polycomb Repressive Complex 2(PRC2)in regulating kernel development.In this study,we applied a reverse genetics approach to investigate the role of ZmFIE1,the homolog of the PRC2 complex component Extra sex combs(Esc),in maize development.The functional loss of ZmFIE1 significantly reduces embryo size in the early stage but has a relatively small impact on mature kernels.Integrating transcriptional and metabolomic profiling suggests that ZmFIE1 is involved in regulating nutrient balance between the endosperm and embryo.In addition,we demonstrate that ZmFIE1 is maternally expressed,and that the maternal inheritance of the fie1 allele significantly affects the imprinting status of paternally imprinted genes.Overall,our results suggest that ZmFIE1 is a key gene involved in the modulation of embryo development via regulating genomic imprinting and nutrient balance between embryo and endosperm,which provides new insights into the regulation mechanism underlying kernel development.
基金supported by the Fuxiaquan Collaborative Innovation Platform(No.K30001)Major Scientific Research Program for Young and Middle-aged Health Professionals of Fujian Province,China(No.2022ZQNZD007)Youth Innovation Technology Project of Higher School in Shandong Province(Food Nanotechnology Innovation Team)。
文摘Quantitative determination of tetracycline(TC)in environment and foods is of great importance,as excessive residues might have negative effects on human health and environmental risks.Herein,a selfpowered molecularly imprinted photoelectrochemical(PEC)sensor based on the Zn O/C photoanode and the Fe-doped CuBi_(2)O_(4)(CBFO)photocathode is developed for the sensitive detection of TC.The photocathodic current can be amplified by the efficient electron transfer caused by the Fermi energy level gap between the photoanode and photocathode.Furthermore,molecularly imprinted polymers(MIPs)at photocathode can selectivity identify the TC templates and thus improve the specificity.Under the optimal conditions,the sensor has a linear range of 10^(-2)-1.0×10^(5) nmol/L,and a limit of detection(LOD)of 0.007 nmol/L(S/N=3).More crucially,the milk sample detection is carried out using the as-prepared sensor,and the outcome is satisfactory.The research gives us a novel sensing platform for quick and accurate antibiotic(like TC)in environment and food monitoring.
基金Supported by National Natural Science Foundation of China(31660402)Industry Technological System Construction Project of Department of Agriculture of Yunnan ProvinceFund for Workstation of Academician Guan Chunyun from Department of Science and Technology of Yunnan Province~~
文摘Genomic imprinting is the epigenetic phenomenon by which certain genes are expressed in a parent-of-origin-specific manner, and was first discovered in mammalian embryos. Recent studies have shown that it also occurs in developing plant seeds, and is now becoming a hot topic of biology of plant seed development. According to the previous studies on imprinted genes, imprinting mechanism and their roles in plant seed development, the current progress of genomic imprinting in plant seed development was summarized and possible strategies were proposed to deal with the problems, which could provide helpful information for further research.
文摘A novel Ni（Ⅱ） ion-imprinted silica gel polymer was prepared via the surface imprinting technique combined with aqueous solution polymerization by using 2-acrylamido-2-methyl-1-propanesulfonic acid（AMPS） as a functional monomer for the selective separation of Ni（Ⅱ） from aqueous solution. The sorbent showed good chemical and thermal stability. Kinetics studies indicated that the equilibrium adsorption was achieved within 10 min and the adsorption kinetics fitted well with the pseudo-second-order kinetic model. The maximum adsorption capacity of the ion-imprinted polymer towards Ni（Ⅱ） at the optimal p H of 7.0 was 66.22 mg·g^（-1）. The relative selectivity coefficients of the sorbent were 9.23, 15.71, 14.72 and 20.15 for Ni（Ⅱ）/Co（Ⅱ）, Ni（Ⅱ）/Cu（Ⅱ）, Ni（Ⅱ）/Zn（Ⅱ） and Ni（Ⅱ）/Pb（Ⅱ）, respectively. The adsorption isotherm fitted well with Langmuir isotherm model. The thermodynamic results indicated that the adsorption of Ni（Ⅱ） was a spontaneous and endothermic process. The sorbent showed good reusability evidenced by six cycles of adsorption/desorption experiments. The precision of this method is satisfactory. Thus, the prepared sorbent can be considered as a promising sorbent for selective separation of Ni（Ⅱ） in real water samples.
文摘Epigenetic dysregulation comprising DNA hypermethylation and hypomethylation, enhancer of zeste homologue 2 （EZH2） overexpression and altered patterns of histone modifications is associated with the progression of prostate cancer. DNA methylation, EZH2 and histone modifications also ensure the parental-specific monoallelic expression of at least 62 imprinted genes. Although it is therefore tempting to speculate that epigenetic dysregulation may extend to imprinted genes, expression changes in cancerous prostates are only well documented for insulin-like growth factor 2 （IGF2）. A literature and database survey on imprinted genes in prostate cancer suggests that the expression of most imprinted genes remains unchanged despite global disturbances in epigenetic mechanisms. Instead, selective genetic and epigenetic changes appear to lead to the inactivation of a sub-network of imprinted genes, which might function in the prostate to limit cell growth induced via the PI3K/Akt pathway, modulate androgen responses and regulate differentiation. Whereas dysregulation of IGF2 may constitute an early change in prostate carcinogenesis, inactivation of this imprinted gene network is rather associated with cancer progression.
文摘This study demonstrates the preparation and characterization of a novel ion imprinted cryogel which exhibits high affinity and selectivity towards Ce（Ⅲ） ions in aqueous solutions and bastnasite ore samples.2-Hydroxyethyl methacrylate（HEMA） and N-methacryloylamido antipyrine（MAAP） were used as functional monomers for the preparation of Ce（Ⅲ） imprinted cryogel. The effects of various factors such as initial Ce（Ⅲ） concentration, flow rate, pH, interaction time and ionic strength on the Ce（Ⅲ） binding to the prepared ion imprinted cryogels were also studied. The binding equilibrium for Ce（Ⅲ） is obtained in30 min at the flow rate of 0.5 mL/min. The maximum binding capacity of the prepared ion imprinted cryogel towards Ce（Ⅲ） is obtained as 36.58 mg/g at optimum conditions. The selectivity of the prepared ion imprinted cryogel towards Ce（Ⅲ） in the presence of other possible interfering lanthanide ions such as La（Ⅲ） and Nd（Ⅲ） were also performed. The obtained results showed that the prepared ion imprinted cryogel exhibits high selectivity and sensitivity towards Ce（Ⅲ） ions. The limit of detection（LOD） was found as 50 μg/L.
基金supported by the Ministry of Agriculture of China(2016ZX08009003-006)National Key R&D Program of China(2019YFA0110700)+1 种基金Science&Technology Department of Yunnan Province(2019HA003)Animal Branch of the Germplasm Bank of Wild Species,Chinese Academy of Sciences(Large Research Infrastructure Funding)。
文摘DEAR EDITOR,Genomic imprinting often results in parent-of-origin specific differential expression of maternally and paternally inherited alleles and plays an essential role in mammalian development and growth.Mammalian genomic imprinting has primarily been studied in mice and humans,with only limited information available for pigs.To systematically characterize this phenomenon and evaluate imprinting status between different species,we investigated imprinted genes on a genome-wide scale in pig brain tissues.
文摘Neural stem cells(NSCs) and imprinted genes play an important role in brain development. On historical grounds, these two determinants have been largely studied independently of each other. Recent evidence suggests, however, that NSCs can reset select genomic imprints to prevent precocious depletion of the stem cell reservoir. Moreover, imprinted genes like the transcriptional regulator Zac1 can fine tune neuronal vs astroglial differentiation of NSCs. Zac1 binds in a sequence-specific manner to pro-neuronal and imprinted genes to confer transcriptional regulation and furthermore coregulates members of the p53-family in NSCs. At the genome scale, Zac1 is a central hub of an imprinted gene network comprising genes with animportant role for NSC quiescence, proliferation and differentiation. Overall, transcriptional, epigenomic, and genomic mechanisms seem to coordinate the functional relationships of NSCs and imprinted genes from development to maturation, and possibly aging.
基金the National Science Foundation of China(Nos21175143 and 20905073)for the financial support of this work
文摘In this work,the quercetin imprinted core-shell microspheres were prepared using silica surface imprinting technique.A simple sol-gel procedure was used for the synthesis of the imprinted materials with 3-aminopropyltriethoxysilane as functional monomer and tetraethyl orthosilicate as crosslinker.The SEM images indicated that the MIPs shell was successfully grafted onto the silica surface.The characteristics of the molecularly imprinted polymers such as capacity,selectivity and absorption dynamic were investigated by rebinding experiments.The results showed that the prepared MIPs had good imprinting effect and adsorption amount of quercetin.
基金Project supported by the Department of Science and Technology of Zhejiang Province(No.2013C02022-2/01),China
文摘A pre-treatment methodology for clenbuterol hydrochloride （CLEN） isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers （MIPs）. CLEN-imprinted pol- ymers were synthesized by the combined use of ally-β-cyclodextrin （ally-13-CD） and methacrylic acid （MAA）, allyl-β-CD and acrylonitrile （AN）, and allyl-β-CD and methyl methacrylate （MMA） as the binary functional monomers. MAA-linked allyl-β-CD MIPs （M-MAA） were characterized by Fourier transform-infrared （FT-IR） spectroscopy and a scanning electron microscope （SEM）. Based upon the results, M-MAA polymers generally proved to be an excellent selective extraction compared to its references： AN-linked allyl-β-CD MIPs （M-AN） and MMA-linked allyl-β-CD MIPs （M-MMA）. M-MAA polymers were eventually chosen to run through a molecularly imprinted solid-phase extraction （MISPE） micro-column to enrich CLEN residues spiked in pig livers. A high recovery was achieved, ranging from 91.03% to 96.76% with relative standard deviation （RSD） ≤4.45%.
文摘As a novel technique, molecular imprinting technique are attracting more and more attention in recent years, due to its specific recognition function to im- printed molecules. This paper firstly introduced this technique from its history, princi- ple, classification and methods, and then reviewed the application in food industry, and presented possible future research orientations.
基金Financial supported by the Natural Science Foundation of China (No: 50003006)
文摘The uniform surface ion-imprinted resins for Zn2+ as the imprinting vip were prepared by emulsifier-free emulsion polymerization utilizing ally phenyl hydrogenphosphate as a functional comonomer. The Zn2+-imprinted resin adsorbed Zn2+ much more effectively than did the unimprinted one. The selective feature of the surface imprinted resins to the template ions was demonstrated.
文摘Heavy metal ion is one of the major environmental pollutants.In this study,a Cu(Ⅱ)ions imprinted magnetic chitosan beads are prepared to use chitosan as functional monomer,Cu(Ⅱ)ions as template,Fe_(3)O_(4) as magnetic core and epichlorohydrin and glutaraldehyde as crosslinker,which can be used for removal Cu(Ⅱ)ions from wastewater.The kinetic study shows that the adsorption process follows the pseudosecond-order kinetic equations.The adsorption isotherm study shows that the Langmuir isotherm equation best fits for the monolayer adsorption processes.The selective adsorption properties are performed in Cu(Ⅱ)/Zn(Ⅱ),Cu(Ⅱ)/Ni(Ⅱ),and Cu(Ⅱ)/Co(Ⅱ)binary systems.The results shows that the ⅡMCD has a high selectivity for Cu(Ⅱ)ions in binary systems.The mechanism of ⅡMCD recognition Cu(Ⅱ)ions is also discussed.The results show that the ⅡMCD adsorption Cu(Ⅱ)ions is an enthalpy controlled process.The absolute value of DH(Cu(Ⅱ))and DS(Cu(Ⅱ))is greater than DH(Zn(Ⅱ),Ni(Ⅱ),Co(Ⅱ))and DS(Zn(Ⅱ),Ni(Ⅱ),Co(Ⅱ)),respectively,this indicates that the Cu(Ⅱ)ions have a good spatial matching with imprinted holes on ⅡMCD.The FTIR and XPS also demonstrates the strongly combination of function groups on imprinted holes in the suitable space position.Finally,the ⅡMCD can be regenerated and reused for 10 times without a significantly decreasing in adsorption capacity.This information can be used for further application in the selective removal of Cu(Ⅱ)ions from industrial wastewater.
