Twelve isolates of Curvularia lunata and six related species from maize or other host plants are examined for the analysis of polyacrylamide gel electrophoresis(SDS PAGE) patterns of their soluble proteins. Difference...Twelve isolates of Curvularia lunata and six related species from maize or other host plants are examined for the analysis of polyacrylamide gel electrophoresis(SDS PAGE) patterns of their soluble proteins. Differences exist significantly among different species and the same species. There is a special protein band for Curvularia at relative mobility value 0.177 (Rf 0.177) and a special protein band for C. lunata at Rf 0.225. Two kinds of primary antibodies to C. lunata are used to indirect ELISA and Avidin Biotin peroxidase Complex (ABC) immunoassay to evaluate their detecting sensitivity and specificity. The sensitivity of ABC immuno assay is higher than indirect ELISA. APAbs to C. lunata is special to C. lunata.展开更多
The relative molecular mass of Arbutin is small. Both fluorolabeling and radiolabeling may affect its properties and functions. Therefore, the immunoassay of Arbutin was studied. Arbutin was coupled to bovine serum al...The relative molecular mass of Arbutin is small. Both fluorolabeling and radiolabeling may affect its properties and functions. Therefore, the immunoassay of Arbutin was studied. Arbutin was coupled to bovine serum albumin to get the Arbutin\|BSA conjugate with high molar ratio of Arbutin to BSA. Two rabbits were injected with the conjugate to develop the anti\|Arbutin serum. Ammonium sulfate precipitation and affinity chromatography were used to purify the antibody. Double agar diffusion test and enzyme\|linked immunosorbent assay (ELISA) were adopted to identify the antibody titer. The results demonstrated that the purity and activity of the antibody are high. The method proposed is satisfactory for the immunological detection of Arbutin.展开更多
文摘Twelve isolates of Curvularia lunata and six related species from maize or other host plants are examined for the analysis of polyacrylamide gel electrophoresis(SDS PAGE) patterns of their soluble proteins. Differences exist significantly among different species and the same species. There is a special protein band for Curvularia at relative mobility value 0.177 (Rf 0.177) and a special protein band for C. lunata at Rf 0.225. Two kinds of primary antibodies to C. lunata are used to indirect ELISA and Avidin Biotin peroxidase Complex (ABC) immunoassay to evaluate their detecting sensitivity and specificity. The sensitivity of ABC immuno assay is higher than indirect ELISA. APAbs to C. lunata is special to C. lunata.
文摘The relative molecular mass of Arbutin is small. Both fluorolabeling and radiolabeling may affect its properties and functions. Therefore, the immunoassay of Arbutin was studied. Arbutin was coupled to bovine serum albumin to get the Arbutin\|BSA conjugate with high molar ratio of Arbutin to BSA. Two rabbits were injected with the conjugate to develop the anti\|Arbutin serum. Ammonium sulfate precipitation and affinity chromatography were used to purify the antibody. Double agar diffusion test and enzyme\|linked immunosorbent assay (ELISA) were adopted to identify the antibody titer. The results demonstrated that the purity and activity of the antibody are high. The method proposed is satisfactory for the immunological detection of Arbutin.
