EVI2A has emerged as a significant biomarker in various diseases;however,its biological role and mechanism in kidney renal clear cell carcinoma(KIRC)remains unexplored.We used TCGA and GEO databases to analyze EVI2A g...EVI2A has emerged as a significant biomarker in various diseases;however,its biological role and mechanism in kidney renal clear cell carcinoma(KIRC)remains unexplored.We used TCGA and GEO databases to analyze EVI2A gene expression comprehensively and performed pan-cancer assessments.Clinical relevance was evaluated through Kaplan-Meier analysis and ROC curves.The gene’s immune relevance was explored through analyses of the tumor microenvironment(TME),Tumor Immune Single-cell Hub(TISCH),immune checkpoints,and immunotherapy sensitivity.Our results indicate that EVI2A expression is upregulated in KIRC,showing correlations with tumor grade and T/N/M stage.EVI2A demonstrates high diagnostic accuracy(AUC=0.906)and predicts poor overall and progression-free survival in KIRC patients.Furthermore,EVI2A expression exhibits significant associations with immunity,including TME scores and specific immune cell types such as Tfh cells,CD4 memory T cells,and CD8+T cells.Elevated EVI2A expression suggests increased sensitivity to PD-1/CTLA-4 and tyrosine kinase inhibitors.In vitro assays confirmed the impact of EVI2A on KIRC behavior,with its knockdown resulting in reduced cell proliferation and migration.In conclusion,our comprehensive analysis identifies EVI2A as a promising biomarker and a novel therapeutic target for intervening in KIRC.These findings hold significant implications for further research and potential clinical applications.展开更多
Salivary cortisol role in response to strong stressors implied in extreme exercises and in sport practice was investigated with the aim to verify the claimed benefits that steers winter swimmers to self-prescribe the ...Salivary cortisol role in response to strong stressors implied in extreme exercises and in sport practice was investigated with the aim to verify the claimed benefits that steers winter swimmers to self-prescribe the trials. Specific biochemical data allow to study a variety of stressors in sports and physical exercises, including extreme ones as winter swimming. Salivary cortisol behavior was examined in winter swimmers trials and canoe, canoe-polo competitions and comparisons of results between days with and without performances were reported. Cortisol circadian rhythm in sedentary subjects was collected as control. All the subjects were selected after anamnestic-clinical checks to evaluate their physiological conditions. The circadian cortisol behavior was reported in days with competitions and trials as well as between these events. Abrupt cortisol concentration changes were detected at the time of the trials and competitions: surprisingly, large increasing and decreasing concentrations were detected in both groups. Moreover, in winter swimmers, cortisol concentration remained fairly elevated in the evening of the trial days. In days without competitions, the usual cortisol circadian rhythm was recovered in sportsmen whereas cortisol concentrations persisted at high levels up to the evening in winter swimmers. The view that an extreme sport-like exercise as the winter swimming may well pose some treats ranging from subclinical aspects up to dismetabolic pathologies and even cardiovascular risks is strengthened by results of cortisol trends, suggesting to check physiological conditions. Results demonstrate that well-being feeling can be in contrast to the claimed improvements of health.展开更多
基金the Ethics Committee of the First Affiliated Hospital of Nanchang University(Approval:(2023)CDYFYYLK(03-013)).
文摘EVI2A has emerged as a significant biomarker in various diseases;however,its biological role and mechanism in kidney renal clear cell carcinoma(KIRC)remains unexplored.We used TCGA and GEO databases to analyze EVI2A gene expression comprehensively and performed pan-cancer assessments.Clinical relevance was evaluated through Kaplan-Meier analysis and ROC curves.The gene’s immune relevance was explored through analyses of the tumor microenvironment(TME),Tumor Immune Single-cell Hub(TISCH),immune checkpoints,and immunotherapy sensitivity.Our results indicate that EVI2A expression is upregulated in KIRC,showing correlations with tumor grade and T/N/M stage.EVI2A demonstrates high diagnostic accuracy(AUC=0.906)and predicts poor overall and progression-free survival in KIRC patients.Furthermore,EVI2A expression exhibits significant associations with immunity,including TME scores and specific immune cell types such as Tfh cells,CD4 memory T cells,and CD8+T cells.Elevated EVI2A expression suggests increased sensitivity to PD-1/CTLA-4 and tyrosine kinase inhibitors.In vitro assays confirmed the impact of EVI2A on KIRC behavior,with its knockdown resulting in reduced cell proliferation and migration.In conclusion,our comprehensive analysis identifies EVI2A as a promising biomarker and a novel therapeutic target for intervening in KIRC.These findings hold significant implications for further research and potential clinical applications.
文摘Salivary cortisol role in response to strong stressors implied in extreme exercises and in sport practice was investigated with the aim to verify the claimed benefits that steers winter swimmers to self-prescribe the trials. Specific biochemical data allow to study a variety of stressors in sports and physical exercises, including extreme ones as winter swimming. Salivary cortisol behavior was examined in winter swimmers trials and canoe, canoe-polo competitions and comparisons of results between days with and without performances were reported. Cortisol circadian rhythm in sedentary subjects was collected as control. All the subjects were selected after anamnestic-clinical checks to evaluate their physiological conditions. The circadian cortisol behavior was reported in days with competitions and trials as well as between these events. Abrupt cortisol concentration changes were detected at the time of the trials and competitions: surprisingly, large increasing and decreasing concentrations were detected in both groups. Moreover, in winter swimmers, cortisol concentration remained fairly elevated in the evening of the trial days. In days without competitions, the usual cortisol circadian rhythm was recovered in sportsmen whereas cortisol concentrations persisted at high levels up to the evening in winter swimmers. The view that an extreme sport-like exercise as the winter swimming may well pose some treats ranging from subclinical aspects up to dismetabolic pathologies and even cardiovascular risks is strengthened by results of cortisol trends, suggesting to check physiological conditions. Results demonstrate that well-being feeling can be in contrast to the claimed improvements of health.
