The present experiment employed the immunohistochemical technique and morphological observation to investigate the expression and distribution of C3, C4, IgG, IgE and 5-HT in portal hypertensive pigs with pathological...The present experiment employed the immunohistochemical technique and morphological observation to investigate the expression and distribution of C3, C4, IgG, IgE and 5-HT in portal hypertensive pigs with pathological change of gastric mucosa and gastric parietal vessels. The wall of gastric mucosal microvessels in portal hypertensive pigs had a positive or strong positive reaction of C3,C4, IgG, IgE and 5-HT with obvious injury of gastric mucosa normal pigs imparted negative or feeble positive reaction, suggesting that during portal hypertension, the gastric mucosal micr0-vessels has deposit of immunocomplexes resulting in the injury of the micro-vessels. It might be a factor inv01ved in the pathogenesis of the gastric mucosal lesion during portal hypertension with cirrhosis.展开更多
Surface Enhanced Raman Spectroscopy(SERS) is a widely used spectroscopic method that can dramatically increase the sensitivity of Raman spectroscopy and has demonstrated significant benefit in the identification of bi...Surface Enhanced Raman Spectroscopy(SERS) is a widely used spectroscopic method that can dramatically increase the sensitivity of Raman spectroscopy and has demonstrated significant benefit in the identification of biological molecules. We report the use of SERS in differentiating the bound immunocomplex of myeloperoxidase(MPO) and its antibody from the unbound complex and its individual components. The SERS signal was enabled by gold nanoparticles attached to MPO, p Ab and their immunocomplex at an excitation wavelength of 785 nm. The obtained SERS spectrum of MPO is in agreement with previous literature. Comparative SERS spectrum analysis of MPO, p Ab, and their immunocomplex reveals the significant peak shifts and intensity variations caused by the conformational changes due to the immunocomplex formation. Several key areas have been identified which correspond to specific amino acids being shielded from undergoing resonance while new amino acid residues are made visible in the SERS spectrum of the immunocomplex and could be a result of conformational binding. Our work demonstrates the capability of SERS to identify binding events and differentiate an immunocomplex from its unbound components with direct applications in biosensors.展开更多
In pH 7.2 Na2HPO4-NaH2PO4 buffer solution and in the （GABF） was combined with human factor B （BF） specifically, presence of PEG-6000, goat-anti-human factor B and aggregated to form immune complex particles that e...In pH 7.2 Na2HPO4-NaH2PO4 buffer solution and in the （GABF） was combined with human factor B （BF） specifically, presence of PEG-6000, goat-anti-human factor B and aggregated to form immune complex particles that exhibited a resonance scattering （RS） peak at 400 nm. The laser scattering indicated that the average diameter of immune complex particles was 1320 nm. BF in the concentration range of 0.04 to 9.60 μg/mL was proportional to the resonance scattering intensity at 400 nm. Its regression equation was A/=33.61C ＋ 1.4, with a correlation coefficient of 0.9969, and a detection limit of 0.01 μg/mL BF. This label-free resonance scattering spectral （RSS） method has been applied to the determination of BF in serum samples, and the results were in agreement with that of the immunoturbity.展开更多
文摘The present experiment employed the immunohistochemical technique and morphological observation to investigate the expression and distribution of C3, C4, IgG, IgE and 5-HT in portal hypertensive pigs with pathological change of gastric mucosa and gastric parietal vessels. The wall of gastric mucosal microvessels in portal hypertensive pigs had a positive or strong positive reaction of C3,C4, IgG, IgE and 5-HT with obvious injury of gastric mucosa normal pigs imparted negative or feeble positive reaction, suggesting that during portal hypertension, the gastric mucosal micr0-vessels has deposit of immunocomplexes resulting in the injury of the micro-vessels. It might be a factor inv01ved in the pathogenesis of the gastric mucosal lesion during portal hypertension with cirrhosis.
基金supported by the W.M.Keck Institute for Attofluidics at Drexel University
文摘Surface Enhanced Raman Spectroscopy(SERS) is a widely used spectroscopic method that can dramatically increase the sensitivity of Raman spectroscopy and has demonstrated significant benefit in the identification of biological molecules. We report the use of SERS in differentiating the bound immunocomplex of myeloperoxidase(MPO) and its antibody from the unbound complex and its individual components. The SERS signal was enabled by gold nanoparticles attached to MPO, p Ab and their immunocomplex at an excitation wavelength of 785 nm. The obtained SERS spectrum of MPO is in agreement with previous literature. Comparative SERS spectrum analysis of MPO, p Ab, and their immunocomplex reveals the significant peak shifts and intensity variations caused by the conformational changes due to the immunocomplex formation. Several key areas have been identified which correspond to specific amino acids being shielded from undergoing resonance while new amino acid residues are made visible in the SERS spectrum of the immunocomplex and could be a result of conformational binding. Our work demonstrates the capability of SERS to identify binding events and differentiate an immunocomplex from its unbound components with direct applications in biosensors.
基金Acknowledgement This work was supported by the National Natural Science Foundation of China （Nos. 21165005, 21075023）, the fund of State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China （CMEMR2011-10）.
文摘In pH 7.2 Na2HPO4-NaH2PO4 buffer solution and in the （GABF） was combined with human factor B （BF） specifically, presence of PEG-6000, goat-anti-human factor B and aggregated to form immune complex particles that exhibited a resonance scattering （RS） peak at 400 nm. The laser scattering indicated that the average diameter of immune complex particles was 1320 nm. BF in the concentration range of 0.04 to 9.60 μg/mL was proportional to the resonance scattering intensity at 400 nm. Its regression equation was A/=33.61C ＋ 1.4, with a correlation coefficient of 0.9969, and a detection limit of 0.01 μg/mL BF. This label-free resonance scattering spectral （RSS） method has been applied to the determination of BF in serum samples, and the results were in agreement with that of the immunoturbity.
