We report an overlapping sampling scheme to accelerate computational ghost imaging for imaging moving targets,based on reordering a set of Hadamard modulation matrices by means of a heuristic algorithm. The new conden...We report an overlapping sampling scheme to accelerate computational ghost imaging for imaging moving targets,based on reordering a set of Hadamard modulation matrices by means of a heuristic algorithm. The new condensed overlapped matrices are then designed to shorten and optimize encoding of the overlapped patterns, which are shown to be much superior to the random matrices. In addition, we apply deep learning to image the target, and use the signal acquired by the bucket detector and corresponding real image to train the neural network. Detailed comparisons show that our new method can improve the imaging speed by as much as an order of magnitude, and improve the image quality as well.展开更多
Fluorescence lifetime imaging microscopy(FLIM)is increasingly used in biomedicine,material science,chemistry,and other related research fields,because of its advantages of high specificity and sensitivity in monitorin...Fluorescence lifetime imaging microscopy(FLIM)is increasingly used in biomedicine,material science,chemistry,and other related research fields,because of its advantages of high specificity and sensitivity in monitoring cellular microenvironments,studying interaction between proteins,metabolic state,screening drugs and analyzing their efficacy,characterizing novel materials,and diagnosing early cancers.Understandably,there is a large interest in obtaining FLIM data within an acquisition time as short as possible.Consequently,there is currently a technology that advances towards faster and faster FLIM recording.However,the maximum speed of a recording technique is only part of the problerm.The acquisition time of a FLIM image is a complex function of many factors.These include the photon rate that can be obtained from the sample,the amount of information a technique extracts from the decay functions,the fficiency at which it determines fluorescence decay parameters from the recorded photons,the demands for the accuracy of these parameters,the number of pixels,and the lateral and axial resolutions that are obtained in biological materials.Starting from a discussion of the parameters which determine the acquisition time,this review will describe existing and emerging FLIM techniques and data analysis algo-rithms,and analyze their performance and recording speed in biological and biomedical applications.展开更多
Based on a digital image correlation（DIC）method with the measurements of a high speed crack＇s displacement and strain fields,a technique to accurately and automatically locate its crack tip has been developed.The c...Based on a digital image correlation（DIC）method with the measurements of a high speed crack＇s displacement and strain fields,a technique to accurately and automatically locate its crack tip has been developed.The crack tip is identified by finding the abrupt jump on the opening（or dislocation）curve of a point on the trace of the crack propagation,while the opening is measured through a virtual extensometer technique and the abrupt jump is identified by finding the peak on the curve.The method was verified using a specially designed experiment and applied to measure the critical crack tip opening angle of a rock sample.Because the involvement of analytical models has been avoided and then the good performance could be ensured for low resolution speckle images,this technique is expected to be very useful in the quantitative study of high speed cracks in experiments using high speed cameras.展开更多
Time-resolved volumetric fluorescence imaging over an extended duration with high spatial/temporal resolution is a key driving force in biomedical research for investigating spatial-temporal dynamics at organism-level...Time-resolved volumetric fluorescence imaging over an extended duration with high spatial/temporal resolution is a key driving force in biomedical research for investigating spatial-temporal dynamics at organism-level systems,yet it remains a major challenge due to the trade-off among imaging speed,light exposure,illumination power,and image quality.Here,we present a deep-learning enhanced light sheet fluorescence microscopy(LSFM)approach that addresses the restoration of rapid volumetric time-lapse imaging with less than 0.03%light exposure and 3.3%acquisition time compared to a typical standard acquisition.We demonstrate that the convolutional neural network(CNN)-transformer network developed here,namely U-net integrated transformer(UI-Trans),successfully achieves the mitigation of complex noise-scattering-coupled degradation and outperforms state-of-the-art deep learning networks,due to its capability of faithfully learning fine details while comprehending complex global features.With the fast generation of appropriate training data via flexible switching between confocal line-scanning LSFM(LS-LSFM)and conventional LSFM,this method achieves a three-to five-fold signal-to-noise ratio(SNR)improvement and~1.8 times contrast improvement in ex vivo zebrafish heart imaging and long-term in vivo 4D(3D morphology+time)imaging of heartbeat dynamics at different developmental stages with ultra-economical acquisitions in terms of light dosage and acquisition time.展开更多
By exploiting the nonlinear responses of fluorescent probes,the spatial resolution of structured illumination microscopy(SIM)can be further increased.However,the traditional reconstruction method of nonlinear structur...By exploiting the nonlinear responses of fluorescent probes,the spatial resolution of structured illumination microscopy(SIM)can be further increased.However,the traditional reconstruction method of nonlinear structured illumination microscopy(NL-SIM)is very slow due to its complex process,which poses a significant challenge to display super resolution results in real-time.Here,we describe an efficient and robust SIM algorithm that enables rapid and accurate full-process SIM reconstruction.First,we present a fast illumination parameters estimation algorithm based on discrete Fourier transforms that result in a more simplified workflow than that of classical methods.Second,an accelerated NL-SIM reconstruction algorithm is developed by extending a highspeed reconstruction framework,joint space and frequency reconstruction(JSFR),to the NL-SIM.In particular,we provide the open-source MATLAB toolbox of our JSFR-NL-SIM algorithm.The entire image reconstruction process is completed in the milliseconds range,representing a significant time saving for the user.展开更多
Combustion instability of pilot flame has been investigated in a model pilot bluff body stabilized combustor by running the pilot flame only. The primary objectives are to investigate the pilot flame dynamics and to p...Combustion instability of pilot flame has been investigated in a model pilot bluff body stabilized combustor by running the pilot flame only. The primary objectives are to investigate the pilot flame dynamics and to provide bases for the study of the interaction mechanisms between the pilot flame and the main flame. Dynamic pressures are measured by dynamic pressure transduc- ers. A high speed camera with CH＊ bandpass filter is used to capture the pilot flame dynamics. The proper orthogonal decomposition （POD） is used to further analyze the high speed images. With the increase of the pilot fuel mass flow rate, the pilot flame changes from stable to unstable state grad- ually. The combustion instability frequency is 136 Hz when the pilot flame is unstable. Numerical simulation results show that the equivalence ratios in both the shear layer and the recirculation zone increase as the pilot fuel mass flow rate increases. The mechanism of the instability of the pilot flame can be attributed to the coupling between the second order acoustic mode and the unsteady heat release due to symmetric vortex shedding. These results illustrate that the pilot fuel mass flow rate has significant influences on the dynamic stability of the pilot flame.展开更多
The influence of the axial relative motion between the target and the source on ghost imaging(GI) is investigated.Both the analytical and experimental results show that the transverse resolution of GI is reduced as th...The influence of the axial relative motion between the target and the source on ghost imaging(GI) is investigated.Both the analytical and experimental results show that the transverse resolution of GI is reduced as the deviation of the target’s center position from the optical axis or the axial motion range increases. To overcome the motion blur,we propose a deblurring method based on speckle-resizing and speed retrieval, and we experimentally validate its effectiveness for an axially moving target with an unknown constant speed. The results demonstrated here will be very useful to forward-looking GI remote sensing.展开更多
The concept of building up a“well-connected fully-integrated,enviomentfriendly,passenger-oriented,economic-efficient,culturally-rich,intelligent and A convenient”station has been being applied to practice in recent ...The concept of building up a“well-connected fully-integrated,enviomentfriendly,passenger-oriented,economic-efficient,culturally-rich,intelligent and A convenient”station has been being applied to practice in recent years.China State Railway Group Co.Ltd .(hereinafier referred to as CHINA RAILWAY)and the Academyof Arts&Design,Tsinghua University have jointly initiated a series of study projects around the topic of“cultural and artistic expression”aiming to optimize design concepts and explore the design aesthetics of China's railway passenger stations in practice.These projects have achieved some positive results and this paper aims to provide a better analysis of the design concept of railway passenger stations in the new era through ananalysis of those stations' design aesthetic.展开更多
The interaction mechanism of internally-staged-swirling stratified flame is complex,and the pilot flame has a manifest influence on flame stability.To study this,a series of experimental investigations for the pilot f...The interaction mechanism of internally-staged-swirling stratified flame is complex,and the pilot flame has a manifest influence on flame stability.To study this,a series of experimental investigations for the pilot flame has been carried out in a model swirl combustor by only supplying the pilot fuel.The CH*chemiluminescence images of the pilot flame are acquired by a high-speed camera with a CH*bandpass filter,whose dynamic characteristics are identified by image statistical analysis and proper orthogonal decomposition(POD)analysis.And the fast algorithm based on matrix theory proposed in this paper increases the operation efficiency and operability of POD.With the pilot equivalence ratioΦincrease,the pilot flame gradually shows an unstable state,whose POD energy distribution is significantly different.In the unstable state,the flame dynamics include three modes—spiral motion mode,flame shedding mode,and axial oscillation mode,whose formation reasons have also been further analyzed in combination with the experimental characteristics.And the fast Fourier transform(FFT)analysis of the time coefficients for the first four POD modes indicates all the dominant frequency is 280 Hz,which means the model combustor is in resonance.In addition,a sensitivity analysis based on the different image resolutions further reveals the robustness of the POD method and its optimization direction.These results emphasize the important influence of the pilot fuel flow rate on the stability of the pilot flame.展开更多
The effects of the ambient air pressure level on the performance of plasma synthetic jet actuator have been investigated through electrical and optical diagnostics.Pressures from 1 atm down to 0.1 atm were tested with...The effects of the ambient air pressure level on the performance of plasma synthetic jet actuator have been investigated through electrical and optical diagnostics.Pressures from 1 atm down to 0.1 atm were tested with a 10 Hz excitation.The discharge measurement demonstrates that there is a voltage range to make the actuator work reliably.Higher pressure level needs a higher breakdown voltage,and a higher discharge current and energy deposition are produced.But when the actuator works with the maximum breakdown voltage,the fraction of the initial capacitor energy delivered to the arc is almost invariable.This preliminary study also confirms the effectiveness of the plasma synthetic jet at low pressure.Indeed,the maximum velocities of the precursor shock and the plasma jet induced by the actuator with maximum breakdown voltage are independent of the ambient pressure level;reach about 530 and 460 m/s respectively.The mass flux of the plasma jet increases with ambient pressure increasing,but the strength of the precursor shock presents a local maximum at 0.6 atm.展开更多
基金supported by the National Key Research and Development Program of China (Grant Nos. 2017YFA0403301, 2017YFB0503301, and2018YFB0504302)the National Natural Science Foundation of China (Grant Nos. 11991073, 61975229, and Y8JC011L51)+2 种基金the Key Program of CAS (Grant No. XDB17030500)the Civil Space Project (Grant No. D040301)the Science Challenge Project (Grant No. TZ2018005)。
文摘We report an overlapping sampling scheme to accelerate computational ghost imaging for imaging moving targets,based on reordering a set of Hadamard modulation matrices by means of a heuristic algorithm. The new condensed overlapped matrices are then designed to shorten and optimize encoding of the overlapped patterns, which are shown to be much superior to the random matrices. In addition, we apply deep learning to image the target, and use the signal acquired by the bucket detector and corresponding real image to train the neural network. Detailed comparisons show that our new method can improve the imaging speed by as much as an order of magnitude, and improve the image quality as well.
基金support from the National Key R&D Program of China(2017YFA0700500)National Natural Science Foundation of China(61775144/61525503/61620106016/61835009/81727804)+2 种基金(Key)Project of Department of Education of Guangdong Province(2015KGJHZ002/2016KCXTD007)Guangdong Natural Science Foundation(2014A030312008,2017A030310132,2018A030313362)Shenzhen Basic Research Project(JCYJ20170818144012025/JCYJ20170818141701667/JCYJ20170412105003520/JCYJ20150930104948169).
文摘Fluorescence lifetime imaging microscopy(FLIM)is increasingly used in biomedicine,material science,chemistry,and other related research fields,because of its advantages of high specificity and sensitivity in monitoring cellular microenvironments,studying interaction between proteins,metabolic state,screening drugs and analyzing their efficacy,characterizing novel materials,and diagnosing early cancers.Understandably,there is a large interest in obtaining FLIM data within an acquisition time as short as possible.Consequently,there is currently a technology that advances towards faster and faster FLIM recording.However,the maximum speed of a recording technique is only part of the problerm.The acquisition time of a FLIM image is a complex function of many factors.These include the photon rate that can be obtained from the sample,the amount of information a technique extracts from the decay functions,the fficiency at which it determines fluorescence decay parameters from the recorded photons,the demands for the accuracy of these parameters,the number of pixels,and the lateral and axial resolutions that are obtained in biological materials.Starting from a discussion of the parameters which determine the acquisition time,this review will describe existing and emerging FLIM techniques and data analysis algo-rithms,and analyze their performance and recording speed in biological and biomedical applications.
基金Supported by the National Natural Science Foundation of China(11172039,11402023)the Fundamental Research Funding of BIT(20120142021)the State Key Laboratory of Earthquake Dynamics(LED2011B03)
文摘Based on a digital image correlation（DIC）method with the measurements of a high speed crack＇s displacement and strain fields,a technique to accurately and automatically locate its crack tip has been developed.The crack tip is identified by finding the abrupt jump on the opening（or dislocation）curve of a point on the trace of the crack propagation,while the opening is measured through a virtual extensometer technique and the abrupt jump is identified by finding the peak on the curve.The method was verified using a specially designed experiment and applied to measure the critical crack tip opening angle of a rock sample.Because the involvement of analytical models has been avoided and then the good performance could be ensured for low resolution speckle images,this technique is expected to be very useful in the quantitative study of high speed cracks in experiments using high speed cameras.
基金supported by National Natural Science Foundation of China(52122008,52270008,52370003,62025502)Guangdong Introducing Innovative and Entrepreneurial Teams of“The Pearl River Talent Recruitment Program”(2021ZT09X044)Shenzhen Technology University under Grant JSZZ202301010.
文摘Time-resolved volumetric fluorescence imaging over an extended duration with high spatial/temporal resolution is a key driving force in biomedical research for investigating spatial-temporal dynamics at organism-level systems,yet it remains a major challenge due to the trade-off among imaging speed,light exposure,illumination power,and image quality.Here,we present a deep-learning enhanced light sheet fluorescence microscopy(LSFM)approach that addresses the restoration of rapid volumetric time-lapse imaging with less than 0.03%light exposure and 3.3%acquisition time compared to a typical standard acquisition.We demonstrate that the convolutional neural network(CNN)-transformer network developed here,namely U-net integrated transformer(UI-Trans),successfully achieves the mitigation of complex noise-scattering-coupled degradation and outperforms state-of-the-art deep learning networks,due to its capability of faithfully learning fine details while comprehending complex global features.With the fast generation of appropriate training data via flexible switching between confocal line-scanning LSFM(LS-LSFM)and conventional LSFM,this method achieves a three-to five-fold signal-to-noise ratio(SNR)improvement and~1.8 times contrast improvement in ex vivo zebrafish heart imaging and long-term in vivo 4D(3D morphology+time)imaging of heartbeat dynamics at different developmental stages with ultra-economical acquisitions in terms of light dosage and acquisition time.
基金National Natural Science Foundation of China(62435007,62135003,62205267,62205265)National Key Research and Development Program of China(2022YFF0712500,2023YFF0722600)Natural Science Basic Research Program of Shaanxi Province(2022JZ-34,2022JM-321,2024JC-YBMS494)。
文摘By exploiting the nonlinear responses of fluorescent probes,the spatial resolution of structured illumination microscopy(SIM)can be further increased.However,the traditional reconstruction method of nonlinear structured illumination microscopy(NL-SIM)is very slow due to its complex process,which poses a significant challenge to display super resolution results in real-time.Here,we describe an efficient and robust SIM algorithm that enables rapid and accurate full-process SIM reconstruction.First,we present a fast illumination parameters estimation algorithm based on discrete Fourier transforms that result in a more simplified workflow than that of classical methods.Second,an accelerated NL-SIM reconstruction algorithm is developed by extending a highspeed reconstruction framework,joint space and frequency reconstruction(JSFR),to the NL-SIM.In particular,we provide the open-source MATLAB toolbox of our JSFR-NL-SIM algorithm.The entire image reconstruction process is completed in the milliseconds range,representing a significant time saving for the user.
文摘Combustion instability of pilot flame has been investigated in a model pilot bluff body stabilized combustor by running the pilot flame only. The primary objectives are to investigate the pilot flame dynamics and to provide bases for the study of the interaction mechanisms between the pilot flame and the main flame. Dynamic pressures are measured by dynamic pressure transduc- ers. A high speed camera with CH＊ bandpass filter is used to capture the pilot flame dynamics. The proper orthogonal decomposition （POD） is used to further analyze the high speed images. With the increase of the pilot fuel mass flow rate, the pilot flame changes from stable to unstable state grad- ually. The combustion instability frequency is 136 Hz when the pilot flame is unstable. Numerical simulation results show that the equivalence ratios in both the shear layer and the recirculation zone increase as the pilot fuel mass flow rate increases. The mechanism of the instability of the pilot flame can be attributed to the coupling between the second order acoustic mode and the unsteady heat release due to symmetric vortex shedding. These results illustrate that the pilot fuel mass flow rate has significant influences on the dynamic stability of the pilot flame.
基金supported by the Hi-Tech Research and Development Program of China under Grant Project No. 2013AA122901
文摘The influence of the axial relative motion between the target and the source on ghost imaging(GI) is investigated.Both the analytical and experimental results show that the transverse resolution of GI is reduced as the deviation of the target’s center position from the optical axis or the axial motion range increases. To overcome the motion blur,we propose a deblurring method based on speckle-resizing and speed retrieval, and we experimentally validate its effectiveness for an axially moving target with an unknown constant speed. The results demonstrated here will be very useful to forward-looking GI remote sensing.
文摘The concept of building up a“well-connected fully-integrated,enviomentfriendly,passenger-oriented,economic-efficient,culturally-rich,intelligent and A convenient”station has been being applied to practice in recent years.China State Railway Group Co.Ltd .(hereinafier referred to as CHINA RAILWAY)and the Academyof Arts&Design,Tsinghua University have jointly initiated a series of study projects around the topic of“cultural and artistic expression”aiming to optimize design concepts and explore the design aesthetics of China's railway passenger stations in practice.These projects have achieved some positive results and this paper aims to provide a better analysis of the design concept of railway passenger stations in the new era through ananalysis of those stations' design aesthetic.
基金Youth Program of National Natural Science Foundation of China(Grant No.51806219)National Science and Technology Major Project(2017-V-0010)。
文摘The interaction mechanism of internally-staged-swirling stratified flame is complex,and the pilot flame has a manifest influence on flame stability.To study this,a series of experimental investigations for the pilot flame has been carried out in a model swirl combustor by only supplying the pilot fuel.The CH*chemiluminescence images of the pilot flame are acquired by a high-speed camera with a CH*bandpass filter,whose dynamic characteristics are identified by image statistical analysis and proper orthogonal decomposition(POD)analysis.And the fast algorithm based on matrix theory proposed in this paper increases the operation efficiency and operability of POD.With the pilot equivalence ratioΦincrease,the pilot flame gradually shows an unstable state,whose POD energy distribution is significantly different.In the unstable state,the flame dynamics include three modes—spiral motion mode,flame shedding mode,and axial oscillation mode,whose formation reasons have also been further analyzed in combination with the experimental characteristics.And the fast Fourier transform(FFT)analysis of the time coefficients for the first four POD modes indicates all the dominant frequency is 280 Hz,which means the model combustor is in resonance.In addition,a sensitivity analysis based on the different image resolutions further reveals the robustness of the POD method and its optimization direction.These results emphasize the important influence of the pilot fuel flow rate on the stability of the pilot flame.
基金supported by the National Natural Science Foundation of China(Grant No.11372349)the Foundation for the Author of National Excellent Doctor Dissertation of China(Grant No.201058)the Nature Science Fund for Distinguished Young Scholars of National University of Defense Technology,China(Grant No.CJ110101)
文摘The effects of the ambient air pressure level on the performance of plasma synthetic jet actuator have been investigated through electrical and optical diagnostics.Pressures from 1 atm down to 0.1 atm were tested with a 10 Hz excitation.The discharge measurement demonstrates that there is a voltage range to make the actuator work reliably.Higher pressure level needs a higher breakdown voltage,and a higher discharge current and energy deposition are produced.But when the actuator works with the maximum breakdown voltage,the fraction of the initial capacitor energy delivered to the arc is almost invariable.This preliminary study also confirms the effectiveness of the plasma synthetic jet at low pressure.Indeed,the maximum velocities of the precursor shock and the plasma jet induced by the actuator with maximum breakdown voltage are independent of the ambient pressure level;reach about 530 and 460 m/s respectively.The mass flux of the plasma jet increases with ambient pressure increasing,but the strength of the precursor shock presents a local maximum at 0.6 atm.
