目的:对两个非综合征型唇腭裂家系中筛查到的IFT172(intraflagellar transport 172)基因突变进行功能分析,进一步明确IFT172在唇腭裂中的致病机制。方法:对前期工作中鉴定的IFT172突变进行保守性分析和蛋白质结构预测。构建野生型和突...目的:对两个非综合征型唇腭裂家系中筛查到的IFT172(intraflagellar transport 172)基因突变进行功能分析,进一步明确IFT172在唇腭裂中的致病机制。方法:对前期工作中鉴定的IFT172突变进行保守性分析和蛋白质结构预测。构建野生型和突变体质粒,转染人胚胎腭板间充质细胞(human embryonic palatal mesenchyme, HEPM),进行转录组测序并对差异表达基因进行分析。结果:IFT172基因中的两个错义突变位点,c.4163A>G(p.Y1388C)和c.1507A>G(p.R503G),在物种间均高度保守,两个突变均导致蛋白质空间构象改变。转录组测序结果显示,差异表达基因富集于坏死性凋亡、调控细胞多能性等信号通路。结论:IFT172基因错义突变可能通过改变蛋白质构象,影响细胞凋亡和细胞多能性并参与唇腭裂的发生。展开更多
CO_(2)/N_(2) injection in heavy oil reservoirs has been demonstrated to enhance oil recovery(EOR)and facilitate CO_(2) capture,utilization,and storage(CCUS).Interfacial tension(IFT)is a crucial parameter for character...CO_(2)/N_(2) injection in heavy oil reservoirs has been demonstrated to enhance oil recovery(EOR)and facilitate CO_(2) capture,utilization,and storage(CCUS).Interfacial tension(IFT)is a crucial parameter for characterizing oil recovery,but it can be influenced by real-time changes in reservoir pressure and temperature during gas injection.The impact of the CO_(2)/N_(2) ratio on the oil-gas IFT under varying temperature and pressure conditions remains unclear.Therefore,a systematic study was conducted to investigate the effects of multiple parameters on the oil-gas IFT during development processes,and a three-dimensional(3D)database and a regression model of IFT were established using experimental data.The results show that IFT is strongly correlated with density difference,moderately correlated with pressure and CO_(2) proportion,weakly correlated with saturates content and resin content,and nonlinearly correlated with temperature,aromatics content,and asphaltene content,respectively.Moreover,it has been observed that an increase in pressure or CO_(2) proportion can lead to a reduction in IFT.However,the impact of temperature changes on IFT varies across different pressure ranges.We introduce a new parameter,the equivalent interfacial tension pressure during temperature changes(EITP),to characterize this effect and discuss the reasons for the emergence of EITP,providing new insight into optimizing the CO_(2)/N_(2) injection ratio in the reservoir.This study aims to reveal the advantages of oil-gas interface characteristics under the influence of multiple parameters in promoting low-carbon and efficient development of heavy oil reservoirs,and to explore the significance of CO_(2)/N_(2) for enhancing heavy oil recovery.展开更多
In chemical flooding,emulsification and interfacial tension(IFT)reduction are crucial for enhanced oil recovery(EOR).However,the dominant performance parameter and technical limits of surfactants for oil displacement ...In chemical flooding,emulsification and interfacial tension(IFT)reduction are crucial for enhanced oil recovery(EOR).However,the dominant performance parameter and technical limits of surfactants for oil displacement remain underexplored.This study investigated the relationship between the emulsification capability and IFT.Accordingly,the dominant performance parameter and the technical limits of surfactants were determined using oil displacement experiments.Specifically,an analysis of 74 sets of experimental results revealed a shift in the significant correlation between EI(a quantitative measure of emulsification capability)andσat anσvalue of 8.5×10^(-2)mN/m(i.e.,critical valueσ_(c)).Forσ<σ_(c),emulsification capability and IFT function as independent performance parameters.The oil displacement experiments using two surfactants with contrasting EI andσvalues demonstrate that emulsification capability,rather than ultra-low IFT,is the dominant performance parameter.This study determined the technical limit of EI using oil displacement experiments via in-situ emulsification.The experimental results indicate strong correlations of EI with oil displacement and recovery efficiencies.The incremental displacement and recovery efficiencies were employed to quantify the potential of surfactants to enhance oil displacement and recovery efficiencies,respectively.The incremental displacement and recovery efficiencies versus EI curves revealed a critical EI(EI_(c))value of 0.53.When EIEI_(c),their increasing rates slowed down markedly.Therefore,the technical limits of the emulsification capability and IFT of surfactants used in this study are determined at EI≥0.53 andσ≤8.5×10^(-2)mN/m,respectively.展开更多
目的观察纤毛内转运蛋白20(intraflagellar transport 20,IFT20)在卵巢良、恶性肿瘤中的表达,探讨其与卵巢癌发生、发展及预后的关系。方法采用免疫组化SP法检测IFT20在157例卵巢肿瘤组织中的表达,其中浆液性囊腺癌52例、黏液性囊腺癌2...目的观察纤毛内转运蛋白20(intraflagellar transport 20,IFT20)在卵巢良、恶性肿瘤中的表达,探讨其与卵巢癌发生、发展及预后的关系。方法采用免疫组化SP法检测IFT20在157例卵巢肿瘤组织中的表达,其中浆液性囊腺癌52例、黏液性囊腺癌22例、子宫内膜样腺癌14例、透明细胞腺癌10例、交界性卵巢肿瘤25例、良性肿瘤18例及正常卵巢组织16例。结果(1)IFT20在卵巢癌组织(76.5%,75/98)和交界性肿瘤组织(32%,8/25)中的阳性率明显高于卵巢良性肿瘤(16.6%,3/18)和正常卵巢组织(12.5%,2/16),组间差异有统计学意义(P<0.05);(2)IFT20蛋白在不同FIGO分期和组织学分级的卵巢癌组织中的表达差异有显著性(P<0.05),随着FIGO分期和组织学分级的升高,且伴有淋巴结转移的卵巢癌组织中IFT20蛋白的表达显著升高;(3)IFT20蛋白表达强弱与患者生存时间呈负相关(P<0.05)。(4)IFT20蛋白表达与卵巢癌患者年龄无关。结论 IFT20蛋白与卵巢癌的发生、浸润和转移相关,在一定程度上可判断卵巢癌恶性程度和评估临床预后,同时为卵巢癌的靶向治疗提供新靶点。展开更多
基金supported by National Natural Science Foundation of China(52204068 and U20B6003)the Program for Scientific Research Innovation Team of Young Scholars in Colleges and Universities of Shandong Province(2022KJ067)。
文摘CO_(2)/N_(2) injection in heavy oil reservoirs has been demonstrated to enhance oil recovery(EOR)and facilitate CO_(2) capture,utilization,and storage(CCUS).Interfacial tension(IFT)is a crucial parameter for characterizing oil recovery,but it can be influenced by real-time changes in reservoir pressure and temperature during gas injection.The impact of the CO_(2)/N_(2) ratio on the oil-gas IFT under varying temperature and pressure conditions remains unclear.Therefore,a systematic study was conducted to investigate the effects of multiple parameters on the oil-gas IFT during development processes,and a three-dimensional(3D)database and a regression model of IFT were established using experimental data.The results show that IFT is strongly correlated with density difference,moderately correlated with pressure and CO_(2) proportion,weakly correlated with saturates content and resin content,and nonlinearly correlated with temperature,aromatics content,and asphaltene content,respectively.Moreover,it has been observed that an increase in pressure or CO_(2) proportion can lead to a reduction in IFT.However,the impact of temperature changes on IFT varies across different pressure ranges.We introduce a new parameter,the equivalent interfacial tension pressure during temperature changes(EITP),to characterize this effect and discuss the reasons for the emergence of EITP,providing new insight into optimizing the CO_(2)/N_(2) injection ratio in the reservoir.This study aims to reveal the advantages of oil-gas interface characteristics under the influence of multiple parameters in promoting low-carbon and efficient development of heavy oil reservoirs,and to explore the significance of CO_(2)/N_(2) for enhancing heavy oil recovery.
基金funded by a basic research project of SINOPEC(KL22023)。
文摘In chemical flooding,emulsification and interfacial tension(IFT)reduction are crucial for enhanced oil recovery(EOR).However,the dominant performance parameter and technical limits of surfactants for oil displacement remain underexplored.This study investigated the relationship between the emulsification capability and IFT.Accordingly,the dominant performance parameter and the technical limits of surfactants were determined using oil displacement experiments.Specifically,an analysis of 74 sets of experimental results revealed a shift in the significant correlation between EI(a quantitative measure of emulsification capability)andσat anσvalue of 8.5×10^(-2)mN/m(i.e.,critical valueσ_(c)).Forσ<σ_(c),emulsification capability and IFT function as independent performance parameters.The oil displacement experiments using two surfactants with contrasting EI andσvalues demonstrate that emulsification capability,rather than ultra-low IFT,is the dominant performance parameter.This study determined the technical limit of EI using oil displacement experiments via in-situ emulsification.The experimental results indicate strong correlations of EI with oil displacement and recovery efficiencies.The incremental displacement and recovery efficiencies were employed to quantify the potential of surfactants to enhance oil displacement and recovery efficiencies,respectively.The incremental displacement and recovery efficiencies versus EI curves revealed a critical EI(EI_(c))value of 0.53.When EIEI_(c),their increasing rates slowed down markedly.Therefore,the technical limits of the emulsification capability and IFT of surfactants used in this study are determined at EI≥0.53 andσ≤8.5×10^(-2)mN/m,respectively.
文摘目的观察纤毛内转运蛋白20(intraflagellar transport 20,IFT20)在卵巢良、恶性肿瘤中的表达,探讨其与卵巢癌发生、发展及预后的关系。方法采用免疫组化SP法检测IFT20在157例卵巢肿瘤组织中的表达,其中浆液性囊腺癌52例、黏液性囊腺癌22例、子宫内膜样腺癌14例、透明细胞腺癌10例、交界性卵巢肿瘤25例、良性肿瘤18例及正常卵巢组织16例。结果(1)IFT20在卵巢癌组织(76.5%,75/98)和交界性肿瘤组织(32%,8/25)中的阳性率明显高于卵巢良性肿瘤(16.6%,3/18)和正常卵巢组织(12.5%,2/16),组间差异有统计学意义(P<0.05);(2)IFT20蛋白在不同FIGO分期和组织学分级的卵巢癌组织中的表达差异有显著性(P<0.05),随着FIGO分期和组织学分级的升高,且伴有淋巴结转移的卵巢癌组织中IFT20蛋白的表达显著升高;(3)IFT20蛋白表达强弱与患者生存时间呈负相关(P<0.05)。(4)IFT20蛋白表达与卵巢癌患者年龄无关。结论 IFT20蛋白与卵巢癌的发生、浸润和转移相关,在一定程度上可判断卵巢癌恶性程度和评估临床预后,同时为卵巢癌的靶向治疗提供新靶点。
