目的探究细胞凋亡抑制蛋白2(cellular inhibitor of apoptosis protein 2,c-IAP2)在鼻咽癌组织中的表达以及临床意义。方法回顾性分析95例鼻咽癌患者的肿瘤组织和40例正常患者的鼻咽组织及临床预后资料,分别利用RT-PCR、Western印迹以...目的探究细胞凋亡抑制蛋白2(cellular inhibitor of apoptosis protein 2,c-IAP2)在鼻咽癌组织中的表达以及临床意义。方法回顾性分析95例鼻咽癌患者的肿瘤组织和40例正常患者的鼻咽组织及临床预后资料,分别利用RT-PCR、Western印迹以及免疫组织化学染色检测上述组织中c-IAP2 mRNA和蛋白的表达水平;卡方检验探究肿瘤组织中c-IAP2表达与临床病理学特征的关系;采用Kaplan-Meier结合Logrank检验表达不同c-IAP2表达水平患者的生存差异;采用单因素和多因素Cox比例风险回归模型分析影响鼻咽癌患者预后的危险因素。结果c-IAP2 mRNA及蛋白水平在鼻咽癌组织中显著高于正常组织(t=12.62,P<0.001);进一步,免疫组化分析发现c-IAP2在鼻咽癌组织呈高表达,且其高表达与肿瘤T分期、N分期、TNM分期及分化程度显著相关(P均<0.05);生存分析显示c-IAP2低表达组鼻咽癌患者的总生存率显著高于高表达组(χ^(2)=11.86,P=0.001),且c-IAP2高表达是导致鼻咽癌患者总生存期缩短的一个独立危险因素(HR=2.425,95%CI:1.256~4.994,P=0.012)。结论鼻咽癌中c-IAP2的表达水平明显上调,其高表达与患者的不良预后显著相关,对于评估鼻咽癌患者的预后具有潜在的临床应用价值。展开更多
The Autographa californica nucleopolyhedrovirus(AcMNPV) contains three apoptosis suppressor genes:p35,iap1 and iap2.AcMNPV P35 functions as a pancaspase inhibitor,but the function of IAP1 and IAP2 has not been entirel...The Autographa californica nucleopolyhedrovirus(AcMNPV) contains three apoptosis suppressor genes:p35,iap1 and iap2.AcMNPV P35 functions as a pancaspase inhibitor,but the function of IAP1 and IAP2 has not been entirely resolved.In this paper,we analyze the function of IAP1 and IAP2 in detail.AcMNPV with p35-deletion inhibited the apoptosis of BTI-Tn-5B1-4(Tn-Hi5) cells induced by a Helicoverpa armigera single nucleocapsid NPV(HearNPV) infection and rescued the replication of HearNPV and BV production in these cells.Transient-expression experiments indicated that both IAP1 and IAP2 suppress apoptosis of Tn-Hi5 cells during HearNPV infection.Recombinant HearNPVs expressing AcMNPV iap1,iap2 and p35,respectively,not only prevented apoptosis but also allowed HearNPV to replicate in Tn-Hi5 cells.However,the iap1,iap2 and p35 genes when expressed in HearNPV were unable to rescue BV production.These results indicate that both AcMNPV iap1 and iap2 function independently as apoptosis inhibitors of and are potential host range factors.展开更多
文摘目的探究细胞凋亡抑制蛋白2(cellular inhibitor of apoptosis protein 2,c-IAP2)在鼻咽癌组织中的表达以及临床意义。方法回顾性分析95例鼻咽癌患者的肿瘤组织和40例正常患者的鼻咽组织及临床预后资料,分别利用RT-PCR、Western印迹以及免疫组织化学染色检测上述组织中c-IAP2 mRNA和蛋白的表达水平;卡方检验探究肿瘤组织中c-IAP2表达与临床病理学特征的关系;采用Kaplan-Meier结合Logrank检验表达不同c-IAP2表达水平患者的生存差异;采用单因素和多因素Cox比例风险回归模型分析影响鼻咽癌患者预后的危险因素。结果c-IAP2 mRNA及蛋白水平在鼻咽癌组织中显著高于正常组织(t=12.62,P<0.001);进一步,免疫组化分析发现c-IAP2在鼻咽癌组织呈高表达,且其高表达与肿瘤T分期、N分期、TNM分期及分化程度显著相关(P均<0.05);生存分析显示c-IAP2低表达组鼻咽癌患者的总生存率显著高于高表达组(χ^(2)=11.86,P=0.001),且c-IAP2高表达是导致鼻咽癌患者总生存期缩短的一个独立危险因素(HR=2.425,95%CI:1.256~4.994,P=0.012)。结论鼻咽癌中c-IAP2的表达水平明显上调,其高表达与患者的不良预后显著相关,对于评估鼻咽癌患者的预后具有潜在的临床应用价值。
基金Supported by the National Basic Research and Development Program of China (Grant No. 2003CB1140)the National Nature Science Foundation of China (Grant Nos. 30325002 and 30670077)in part by a grant from the Royal Netherlands Academy of Arts and Sciences (KNAW) (Program Strategic Scientific Alliances Project,Grant No. 04-PSA-BD-02)
文摘The Autographa californica nucleopolyhedrovirus(AcMNPV) contains three apoptosis suppressor genes:p35,iap1 and iap2.AcMNPV P35 functions as a pancaspase inhibitor,but the function of IAP1 and IAP2 has not been entirely resolved.In this paper,we analyze the function of IAP1 and IAP2 in detail.AcMNPV with p35-deletion inhibited the apoptosis of BTI-Tn-5B1-4(Tn-Hi5) cells induced by a Helicoverpa armigera single nucleocapsid NPV(HearNPV) infection and rescued the replication of HearNPV and BV production in these cells.Transient-expression experiments indicated that both IAP1 and IAP2 suppress apoptosis of Tn-Hi5 cells during HearNPV infection.Recombinant HearNPVs expressing AcMNPV iap1,iap2 and p35,respectively,not only prevented apoptosis but also allowed HearNPV to replicate in Tn-Hi5 cells.However,the iap1,iap2 and p35 genes when expressed in HearNPV were unable to rescue BV production.These results indicate that both AcMNPV iap1 and iap2 function independently as apoptosis inhibitors of and are potential host range factors.
