The Autographa californica nucleopolyhedrovirus(AcMNPV) contains three apoptosis suppressor genes:p35,iap1 and iap2.AcMNPV P35 functions as a pancaspase inhibitor,but the function of IAP1 and IAP2 has not been entirel...The Autographa californica nucleopolyhedrovirus(AcMNPV) contains three apoptosis suppressor genes:p35,iap1 and iap2.AcMNPV P35 functions as a pancaspase inhibitor,but the function of IAP1 and IAP2 has not been entirely resolved.In this paper,we analyze the function of IAP1 and IAP2 in detail.AcMNPV with p35-deletion inhibited the apoptosis of BTI-Tn-5B1-4(Tn-Hi5) cells induced by a Helicoverpa armigera single nucleocapsid NPV(HearNPV) infection and rescued the replication of HearNPV and BV production in these cells.Transient-expression experiments indicated that both IAP1 and IAP2 suppress apoptosis of Tn-Hi5 cells during HearNPV infection.Recombinant HearNPVs expressing AcMNPV iap1,iap2 and p35,respectively,not only prevented apoptosis but also allowed HearNPV to replicate in Tn-Hi5 cells.However,the iap1,iap2 and p35 genes when expressed in HearNPV were unable to rescue BV production.These results indicate that both AcMNPV iap1 and iap2 function independently as apoptosis inhibitors of and are potential host range factors.展开更多
基金Supported by the National Basic Research and Development Program of China (Grant No. 2003CB1140)the National Nature Science Foundation of China (Grant Nos. 30325002 and 30670077)in part by a grant from the Royal Netherlands Academy of Arts and Sciences (KNAW) (Program Strategic Scientific Alliances Project,Grant No. 04-PSA-BD-02)
文摘The Autographa californica nucleopolyhedrovirus(AcMNPV) contains three apoptosis suppressor genes:p35,iap1 and iap2.AcMNPV P35 functions as a pancaspase inhibitor,but the function of IAP1 and IAP2 has not been entirely resolved.In this paper,we analyze the function of IAP1 and IAP2 in detail.AcMNPV with p35-deletion inhibited the apoptosis of BTI-Tn-5B1-4(Tn-Hi5) cells induced by a Helicoverpa armigera single nucleocapsid NPV(HearNPV) infection and rescued the replication of HearNPV and BV production in these cells.Transient-expression experiments indicated that both IAP1 and IAP2 suppress apoptosis of Tn-Hi5 cells during HearNPV infection.Recombinant HearNPVs expressing AcMNPV iap1,iap2 and p35,respectively,not only prevented apoptosis but also allowed HearNPV to replicate in Tn-Hi5 cells.However,the iap1,iap2 and p35 genes when expressed in HearNPV were unable to rescue BV production.These results indicate that both AcMNPV iap1 and iap2 function independently as apoptosis inhibitors of and are potential host range factors.
