Organic room temperature phosphorescence(RTP)in water has attracted much attention recently for its potential biological applications.However,it remains a formidable challenge to achieve efficient RTP from pure organi...Organic room temperature phosphorescence(RTP)in water has attracted much attention recently for its potential biological applications.However,it remains a formidable challenge to achieve efficient RTP from pure organic compounds in aqueous phase due to the dramatic deactivation of triplet excited states in water and the poor water dispersibility of large organic particles/crystals.Represented herein is covalent incorporation of a pure organic monochromophore in silica nanoparticles(SiNPs)featuring fluorescence and bright phosphorescence in aqueous solution.The covalent bonding of organic phosphors in polysiloxane framework was found to show excellent water dispersibility,at the same time suppress the nonradiative deactivation of triplet excited states especially from water,thus leading to high phosphorescence quantum yields(up to 22%)and long lifetimes(up to 3.5 ms)in aqueous phase.More strikingly,oxygen-insensitive fluorescence as internal reference and oxygen-dependent phosphorescence as oxygen indicator from the organic chromophore in the porous SiNPs realized ratiometric hypoxia detection with ultrasensitivity(K_(SV)=449.3 bar^(-1)).展开更多
Colorectal cancer(CRC)is the third most commonly diagnosed cancer in the world,exhibiting persistently high mortality rates due to delayed diagnosis and imprecise lesion localization.Leveraging the prevalent hypoxic m...Colorectal cancer(CRC)is the third most commonly diagnosed cancer in the world,exhibiting persistently high mortality rates due to delayed diagnosis and imprecise lesion localization.Leveraging the prevalent hypoxic microenvironment characteristic of CRC lesions,this study innovatively developed a PEGylated iridium-based near-infrared(NIR)hypoxia nanoprobe,Ir-PEG.This nanoprobe can be activated in situ under hypoxic conditions,providing high-contrast imaging of colonic lesions,even though the intestine is inherently a low oxygen environment.In vitro evaluation demonstrated that Ir-PEG had excellent oxygen sensitivity,water solubility,and deep tissue penetration capability.These properties enabled Ir-PEG to achieve precise imaging of CRC in the native colonic microenvironment.Remarkably,in both in vitro and in vivo models,Ir-PEG achieved highly sensitive detection of cancer cell populations,and could detect as few as 104 CT26 cells in vivo.In addition,the nanoprobe could successfully identify different tumor types based on differential oxygen consumption rates across various cancer cells,suggesting its potential to identify intratumoral heterogeneity.As a molecular imaging tool,Ir-PEG enabled early non-invasive detection of CRC with high sensitivity and specificity,and holding significant promise for clinical translation.展开更多
基金Beijing Natural Science Foundation,Grant/Award Number:2222033National Natural Science Foundation of China,Grant/Award Numbers:22071258,21871280,22193013,22088102+1 种基金Strategic Priority Research Program of the Chinese Academy of Sciences,Grant/Award Number:XDB17000000Natural Science Foundation of Shanxi Province of China,Grant/Award Number:201901D111138。
文摘Organic room temperature phosphorescence(RTP)in water has attracted much attention recently for its potential biological applications.However,it remains a formidable challenge to achieve efficient RTP from pure organic compounds in aqueous phase due to the dramatic deactivation of triplet excited states in water and the poor water dispersibility of large organic particles/crystals.Represented herein is covalent incorporation of a pure organic monochromophore in silica nanoparticles(SiNPs)featuring fluorescence and bright phosphorescence in aqueous solution.The covalent bonding of organic phosphors in polysiloxane framework was found to show excellent water dispersibility,at the same time suppress the nonradiative deactivation of triplet excited states especially from water,thus leading to high phosphorescence quantum yields(up to 22%)and long lifetimes(up to 3.5 ms)in aqueous phase.More strikingly,oxygen-insensitive fluorescence as internal reference and oxygen-dependent phosphorescence as oxygen indicator from the organic chromophore in the porous SiNPs realized ratiometric hypoxia detection with ultrasensitivity(K_(SV)=449.3 bar^(-1)).
基金supported by the National Key Research and Devel-opment Program of China(2023YFF0715400,2023YFF0715404)the Natural Science Foundation of China(92163214,52333003 and 52373288)+4 种基金Basic Research Program of Jiangsu(BK20244003)the Natural Science Foundation of Jiangsu Province(BK20241237)China Postdoctoral Sci-ence Foundation(2024M761373)Jiangsu Funding Program for Excellent Postdoctoral Talent(2023ZB557)Nanjing University International Collaboration Initiative.
文摘Colorectal cancer(CRC)is the third most commonly diagnosed cancer in the world,exhibiting persistently high mortality rates due to delayed diagnosis and imprecise lesion localization.Leveraging the prevalent hypoxic microenvironment characteristic of CRC lesions,this study innovatively developed a PEGylated iridium-based near-infrared(NIR)hypoxia nanoprobe,Ir-PEG.This nanoprobe can be activated in situ under hypoxic conditions,providing high-contrast imaging of colonic lesions,even though the intestine is inherently a low oxygen environment.In vitro evaluation demonstrated that Ir-PEG had excellent oxygen sensitivity,water solubility,and deep tissue penetration capability.These properties enabled Ir-PEG to achieve precise imaging of CRC in the native colonic microenvironment.Remarkably,in both in vitro and in vivo models,Ir-PEG achieved highly sensitive detection of cancer cell populations,and could detect as few as 104 CT26 cells in vivo.In addition,the nanoprobe could successfully identify different tumor types based on differential oxygen consumption rates across various cancer cells,suggesting its potential to identify intratumoral heterogeneity.As a molecular imaging tool,Ir-PEG enabled early non-invasive detection of CRC with high sensitivity and specificity,and holding significant promise for clinical translation.
