The influence of a 40-fold attenuated geomagnetic field and its combined action with low doses of α- and γ-irradiation on the physiological characteristics of seeds of the highest plants and redox properties of wate...The influence of a 40-fold attenuated geomagnetic field and its combined action with low doses of α- and γ-irradiation on the physiological characteristics of seeds of the highest plants and redox properties of water was investigated. It established the reduction of seed germination both under direct and indirect effects due to water action of attenuated geomagnetic field. A negative effect of hypomagnetic field on grown characteristics of seeds under indirect effect via water was decreased by the low doses of γ-irradiation, and was increased by low doses of α-irradiation, i.e. ionized radiation was the dominant factor in their combined action. It was revealed the increasing of the value of the oxidation-reduction potential of water under the influence of low-intensive α-ir-radiation (239Pu), γ-irradiation (137Cs) and also that the magnetic induction attenuated pointing to a natural decline. The increasing of the oxidation-reduction potential value testifies about “the regular decreasing of internal energy of water molecules” and the increasing of its oxidative properties, which, in our opinion, is caused the inhibition of the germination of seeds. It is supposed that namely water is the main component in the effects of studying factors on bio-objects, which acts due to the alterations of the properties and structural content of water.展开更多
The article represents the generalizing data for the studying of the effect of hypomagnetic field on physico-chemistry properties of water and bio-objects. It was revealed the changing state of water: increasing of it...The article represents the generalizing data for the studying of the effect of hypomagnetic field on physico-chemistry properties of water and bio-objects. It was revealed the changing state of water: increasing of its oxidation-reduction potential and oxidative properties as magnetic induction attenuated pointing to a natural decline, that testifies about the regular decreasing of internal energy of water molecules, which, in our opinion, is caused the inhibition of the germination of seeds of the highest plants, embryonic development of Planorbarius corneus and the changing of energy state of growing mediums for cell culture of mammals. It is supposed that namely the changing state of water is the main component in the effects of weakening of magnetic field on the studying bio-objects.展开更多
Research has shown that the hypomagnetic field(HMF)can affect embryo development,cell proliferation,learning and memory,and in vitro tubulin assembly.In the present study,we aimed to elucidate the molecular mechanism ...Research has shown that the hypomagnetic field(HMF)can affect embryo development,cell proliferation,learning and memory,and in vitro tubulin assembly.In the present study,we aimed to elucidate the molecular mechanism by which the HMF exerts its effect,by comparing the transcriptome profiles of human neuroblastoma cells exposed to either the HMF or the geomagnetic field.A total of 2464 differentially expressed genes(DEGs)were identified,216 of which were up-regulated and2248 of which were down-regulated after exposure to the HMF.These DEGs were found to be significantly clustered into several key processes,namely macromolecule localization,protein transport,RNA processing,and brain function.Seventeen DEGs were verified by real-time quantitative PCR,and the expression levels of nine of these DEGs were measured every 6 h.Most notably,MAPK1 and CRY2,showed significant up-and down-regulation,respectively,during the first 6 h of HMF exposure,which suggests involvement of the MAPK pathway and cryptochrome in the early bio-HMF response.Our results provide insights into the molecular mechanisms underlying the observed biological effects of the HMF.展开更多
Accumulative evidence has shown the adverse effects of a geomagnetic field shielded condition, so called a hypomagnetic field （HMF）, on the metabolic processes and oxidative stress in animals and cells. However, the...Accumulative evidence has shown the adverse effects of a geomagnetic field shielded condition, so called a hypomagnetic field （HMF）, on the metabolic processes and oxidative stress in animals and cells. However, the underlying mechanism remains unclear. In this study, we evaluate the role of HMF on the regulation of cellular reactive oxygen species （ROS） in human neuroblastoma SH-SY5Y cells. We found that HMF exposure led to ROS decrease, and that restoring the decrease by additional H2O2 rescued the HMF-enhanced cell proliferation. The measurements on ROS related indexes, including total anti-oxidant capacity, H2O2 and superoxide anion levels, and superoxide dismutase （SOD） activity and expres- sion, indicated that the HMF reduced H2O2 production and inhibited the activity of CuZn-SOD. Moreover, the HMF accelerated the denaturation of CuZn-SOD as well as enhanced aggregation of CuZn-SOD protein, in vitro. Our findings indicate that CuZn-SOD is able to response to the HMF stress and suggest it a mediator of the HMF effect.展开更多
Living organisms are exposed to the geomagnetic field （GMF） throughout their lifespan. Elimination of the GMF, resulting in a hypogeomagnetic field （HMF）, leads to central nervous system dysfunction and abnormal d...Living organisms are exposed to the geomagnetic field （GMF） throughout their lifespan. Elimination of the GMF, resulting in a hypogeomagnetic field （HMF）, leads to central nervous system dysfunction and abnormal development in animals. However, the cellular mecha-nisms underlying these effects have not been identified so far. Here, we show that exposure to an HMF （〈200 nT）, produced by a magnetic field shielding chamber, pro- motes the proliferation of neural progenitor/stem cells （NPCslNSCs） from C57BL/6 mice. Following seven-day HMF-exposure, the primary neurospheres （NSs） were significantly larger in size, and twice more NPCslNSCs were harvested from neonatal NSs, when compared to the GMF controls. The self-renewal capacity and multi- potency of the NSs were maintalned, as HMF-exposed NSs were posltlve for NSC markers （Nestin and Sox2）, and could differentiate into neurons and astrocytelglial cells and be passaged continuously. In addition, adult mice exposed to the HMF for one month were observed to have a greater number of proliferative cells In the subventrlcular zone. These flndlngs indicate that continuous HMF-exposure increases the proliferation of NPCslNSCs, In vitro and in vlvo. HMF-dlsturbed NPCs/NSCs production probably affects brain development and function, which provides a novel clue for elucidating the cellular mechanisms of the bio.HMF response.展开更多
文摘The influence of a 40-fold attenuated geomagnetic field and its combined action with low doses of α- and γ-irradiation on the physiological characteristics of seeds of the highest plants and redox properties of water was investigated. It established the reduction of seed germination both under direct and indirect effects due to water action of attenuated geomagnetic field. A negative effect of hypomagnetic field on grown characteristics of seeds under indirect effect via water was decreased by the low doses of γ-irradiation, and was increased by low doses of α-irradiation, i.e. ionized radiation was the dominant factor in their combined action. It was revealed the increasing of the value of the oxidation-reduction potential of water under the influence of low-intensive α-ir-radiation (239Pu), γ-irradiation (137Cs) and also that the magnetic induction attenuated pointing to a natural decline. The increasing of the oxidation-reduction potential value testifies about “the regular decreasing of internal energy of water molecules” and the increasing of its oxidative properties, which, in our opinion, is caused the inhibition of the germination of seeds. It is supposed that namely water is the main component in the effects of studying factors on bio-objects, which acts due to the alterations of the properties and structural content of water.
文摘The article represents the generalizing data for the studying of the effect of hypomagnetic field on physico-chemistry properties of water and bio-objects. It was revealed the changing state of water: increasing of its oxidation-reduction potential and oxidative properties as magnetic induction attenuated pointing to a natural decline, that testifies about the regular decreasing of internal energy of water molecules, which, in our opinion, is caused the inhibition of the germination of seeds of the highest plants, embryonic development of Planorbarius corneus and the changing of energy state of growing mediums for cell culture of mammals. It is supposed that namely the changing state of water is the main component in the effects of weakening of magnetic field on the studying bio-objects.
基金supported by the Queensland-Chinese Academy of Sciences(QCAS)Biotechnology Fund(GJHZ1131)the Project of Chinese Academy of Sciences for the Development of Major Scientific Research Equipment(YZ201148)+1 种基金the National Natural Science Foundation of China(31200628)the External Cooperation Program of Bureau of International Cooperation,Chinese Academy of Sciences(GJHZ201302)
文摘Research has shown that the hypomagnetic field(HMF)can affect embryo development,cell proliferation,learning and memory,and in vitro tubulin assembly.In the present study,we aimed to elucidate the molecular mechanism by which the HMF exerts its effect,by comparing the transcriptome profiles of human neuroblastoma cells exposed to either the HMF or the geomagnetic field.A total of 2464 differentially expressed genes(DEGs)were identified,216 of which were up-regulated and2248 of which were down-regulated after exposure to the HMF.These DEGs were found to be significantly clustered into several key processes,namely macromolecule localization,protein transport,RNA processing,and brain function.Seventeen DEGs were verified by real-time quantitative PCR,and the expression levels of nine of these DEGs were measured every 6 h.Most notably,MAPK1 and CRY2,showed significant up-and down-regulation,respectively,during the first 6 h of HMF exposure,which suggests involvement of the MAPK pathway and cryptochrome in the early bio-HMF response.Our results provide insights into the molecular mechanisms underlying the observed biological effects of the HMF.
文摘Accumulative evidence has shown the adverse effects of a geomagnetic field shielded condition, so called a hypomagnetic field （HMF）, on the metabolic processes and oxidative stress in animals and cells. However, the underlying mechanism remains unclear. In this study, we evaluate the role of HMF on the regulation of cellular reactive oxygen species （ROS） in human neuroblastoma SH-SY5Y cells. We found that HMF exposure led to ROS decrease, and that restoring the decrease by additional H2O2 rescued the HMF-enhanced cell proliferation. The measurements on ROS related indexes, including total anti-oxidant capacity, H2O2 and superoxide anion levels, and superoxide dismutase （SOD） activity and expres- sion, indicated that the HMF reduced H2O2 production and inhibited the activity of CuZn-SOD. Moreover, the HMF accelerated the denaturation of CuZn-SOD as well as enhanced aggregation of CuZn-SOD protein, in vitro. Our findings indicate that CuZn-SOD is able to response to the HMF stress and suggest it a mediator of the HMF effect.
文摘Living organisms are exposed to the geomagnetic field （GMF） throughout their lifespan. Elimination of the GMF, resulting in a hypogeomagnetic field （HMF）, leads to central nervous system dysfunction and abnormal development in animals. However, the cellular mecha-nisms underlying these effects have not been identified so far. Here, we show that exposure to an HMF （〈200 nT）, produced by a magnetic field shielding chamber, pro- motes the proliferation of neural progenitor/stem cells （NPCslNSCs） from C57BL/6 mice. Following seven-day HMF-exposure, the primary neurospheres （NSs） were significantly larger in size, and twice more NPCslNSCs were harvested from neonatal NSs, when compared to the GMF controls. The self-renewal capacity and multi- potency of the NSs were maintalned, as HMF-exposed NSs were posltlve for NSC markers （Nestin and Sox2）, and could differentiate into neurons and astrocytelglial cells and be passaged continuously. In addition, adult mice exposed to the HMF for one month were observed to have a greater number of proliferative cells In the subventrlcular zone. These flndlngs indicate that continuous HMF-exposure increases the proliferation of NPCslNSCs, In vitro and in vlvo. HMF-dlsturbed NPCs/NSCs production probably affects brain development and function, which provides a novel clue for elucidating the cellular mechanisms of the bio.HMF response.
