In this study,α-amylase andα-glucosidase inhibition activities of cell-free supernatant(CFS)of 216 lactic acid bacteria were measured to screen lactic acid bacteria resources with potential hypoglycemic functions.Si...In this study,α-amylase andα-glucosidase inhibition activities of cell-free supernatant(CFS)of 216 lactic acid bacteria were measured to screen lactic acid bacteria resources with potential hypoglycemic functions.Significant inhibition activities were detected in Pediococcus acidilactici FM-Pa-JXM94,L15,GC215 and L31,and their probiotic properties were further assessed.Following the inhibitory activities onα-glucosidase andα-amylase,the viability of simulated gastric juice and adhesive ability,FM-Pa-JXM94 was screened and identified as Pediococcus acidilactici by 16S rDNA nucleotide sequencing,and the potential hypoglycemic mechanism was explored using IR-HepG2.CFS,cell-free extract(CFE)and bacterial cells of FM-Pa-JXM94 demonstrated a strong potential to induce glucose consumption,glycogen synthesis,and pyruvate kinase activity,as well as decrease phosphoenolpyruvate carboxykinase activity of IR-HepG2 cells.Additionally,RT-qPCR results showed that CFS,CFE and bacteria cells upregulated the mRNA expression of IRS1,AKT,GYS2 and PI3K genes and downregulated the mRNA expression of GSK3β,FOXO1,PEPCK and G-6-Pase genes of IRS-1/PI3K/AKT/GSK3β/GYS2 and PI3K/AKT/FOXO1/PEPCK-G6Pase signal pathways.These findings illustrate that the different FM-Pa-JXM94 derivatives could alleviate insulin resistance of IR-HepG2 cells from key enzyme activity and gene levels.This study showcases FM-Pa-JXM94 as a promising probiotic candidate for preparing functional foods and hypoglycemic function supplements.展开更多
基金supported by Exploring and Overturning Innovation Program of Jiangsu Academy of Agricultural Sciences(ZX(21)1225)Key R&D Program Project of the Autonomous Region(2023B02028-2)。
文摘In this study,α-amylase andα-glucosidase inhibition activities of cell-free supernatant(CFS)of 216 lactic acid bacteria were measured to screen lactic acid bacteria resources with potential hypoglycemic functions.Significant inhibition activities were detected in Pediococcus acidilactici FM-Pa-JXM94,L15,GC215 and L31,and their probiotic properties were further assessed.Following the inhibitory activities onα-glucosidase andα-amylase,the viability of simulated gastric juice and adhesive ability,FM-Pa-JXM94 was screened and identified as Pediococcus acidilactici by 16S rDNA nucleotide sequencing,and the potential hypoglycemic mechanism was explored using IR-HepG2.CFS,cell-free extract(CFE)and bacterial cells of FM-Pa-JXM94 demonstrated a strong potential to induce glucose consumption,glycogen synthesis,and pyruvate kinase activity,as well as decrease phosphoenolpyruvate carboxykinase activity of IR-HepG2 cells.Additionally,RT-qPCR results showed that CFS,CFE and bacteria cells upregulated the mRNA expression of IRS1,AKT,GYS2 and PI3K genes and downregulated the mRNA expression of GSK3β,FOXO1,PEPCK and G-6-Pase genes of IRS-1/PI3K/AKT/GSK3β/GYS2 and PI3K/AKT/FOXO1/PEPCK-G6Pase signal pathways.These findings illustrate that the different FM-Pa-JXM94 derivatives could alleviate insulin resistance of IR-HepG2 cells from key enzyme activity and gene levels.This study showcases FM-Pa-JXM94 as a promising probiotic candidate for preparing functional foods and hypoglycemic function supplements.
