Bearberry(Arctostaphylos uva-ursi L.)is a perennial plant of the heather family(Ericaceae).The leaves are dominated by arbutin,phenol carbonic acids flavonoids,saponins,etc.It was previously shown that the bearberry l...Bearberry(Arctostaphylos uva-ursi L.)is a perennial plant of the heather family(Ericaceae).The leaves are dominated by arbutin,phenol carbonic acids flavonoids,saponins,etc.It was previously shown that the bearberry leaves extract reduced blood glucose level in healthy animals under glucose overload,so it need to be studied more detail.The aim of the study was to investigate the chemical composition and the effect of dry alcohol extract from bearberry leaves,which enriched with cysteine,on the rats pancreas under experimental dexamethasone-induced insulin resistance(IR).Arctostaphylos uva-ursi L.leaves extract and its modified cysteine preparation were obtained according to the developed method with 50%ethanol solution.Their phytochemical profile,hypoglycaemic and pancreatic protective effect were investigated.Phenologlycoside(arbutin),phenolic carboxylic acid(gallic acid),5 flavonoids and 4 hydroxy-cinnamic acids were identified and quantified in the extracts by HPLC.Present data revealed that bearberry leaves alcoholic dry extract enriched with cysteine has a hypoglycaemic and pancreatic protective effect in treated animals under dexamethasone-induced IR model.Treatment improved hyperglycaemia,insulin resistance and beta cell reduc-tion induced by dexamethasone injections.展开更多
Selenium-enriched polysaccharides refer to polysaccharides that contain selenium,which have emerged as a significant area of research in recent years due to the bioactivities associated with both polysaccharides and s...Selenium-enriched polysaccharides refer to polysaccharides that contain selenium,which have emerged as a significant area of research in recent years due to the bioactivities associated with both polysaccharides and selenium.In this study,we prepared Spirulina platensis polysaccharides(SPP)and selenium-enriched SPP(SeSPP),and assessed their structural properties and hypoglycaemic functions both in vitro and in vivo.The re-sults of the structural characterization indicated that SPP was successfully selenized.The interaction between SPP and selenium primarily occurred in the forms of Se-O-C and O-Se-O through covalent interactions.The molecular weight of SeSPP was found to be more concentrated compared to SPP,and its microscopic morphology exhibited greater complexity,including both rod-shaped and spherical particles.It was determined that SPP and SeSPP were mainly composed of glucose,rhamnose,and fucose.In vitro assays demonstrated that SeSPP exhibited significantly enhanced hypoglycaemic and antioxidant properties compared to SPP.In an in vivo model of streptozotocin-and high-fat diet-induced type 2 diabetes mice,SeSPP improved hypoglycaemic activity by reversing body weight,blood glucose levels,glucose tolerance,insulin resistance,lipid levels,hepatic oxidative stress levels,as well as liver and pancreatic morphology and function.Consequently,SeSPP may serve as a se-lenium supplement that could enhance hypoglycaemic effects.展开更多
Partridge tea is a plant used as a speciality tea drink in Hainan Province,China.The aim of this study was to isolate polyphenols from partridge tea,investigate their main constituent compositions and ameliorative eff...Partridge tea is a plant used as a speciality tea drink in Hainan Province,China.The aim of this study was to isolate polyphenols from partridge tea,investigate their main constituent compositions and ameliorative effects on insulin resistance in insulin resistant HepG2(IR-HepG2)cells.PTP was obtained from partridge tea,then PTP-1,PTP-2 and PTP-3 were obtained after further purification.The polyphenol contents of PTP,PTP-1,PTP-2,PTP-3 were determined to be 15.9%,51.4%,42.51% and 22.74%,respectively.PTP and PTP-1 had similar compositions,and the contents of the main components of PTP-1 was higher than that of PTP.Structural compositions and contents of PTP-2 and PTP-3 differ significantly.Further analysis indicated that PTP failed to have significant hypoglycaemic activity,while other three polyphenols regulated the fat metabolism disorders caused by insulin resistance,thus improving the body’s utilization of blood glucose.In addition,the three polyphenols reversed the damage caused by insulin resistance in IR-HepG2 cells by up-regulating the activities of HK(200μg/mL PTP-1:2.8414 mol/min/mL)and PK(200μg/mL PTP-1:10.7204 U/L),increasing the activity of SOD and CAT and decreasing the level of MDA.The mRNA results showed that PTP-1,PTP-2 and PTP-3 could achieve hypoglycaemic efficacy in the body by regulating the IRS-1/PI3K/AKT signal pathway.In summary,our results suggested that the polyphenols of partridge tea could be a promising natural source for preventing and treating hyperglycemia.展开更多
基金funded by the Ministry of Health Care of Ukraine at the expense of the State Budget in the framework#2301020“Scientific and scientific-technical activity in the field of health protection”on the topic“Modern approaches to the creation of new medicines for a correction of metabolic syndrome”.
文摘Bearberry(Arctostaphylos uva-ursi L.)is a perennial plant of the heather family(Ericaceae).The leaves are dominated by arbutin,phenol carbonic acids flavonoids,saponins,etc.It was previously shown that the bearberry leaves extract reduced blood glucose level in healthy animals under glucose overload,so it need to be studied more detail.The aim of the study was to investigate the chemical composition and the effect of dry alcohol extract from bearberry leaves,which enriched with cysteine,on the rats pancreas under experimental dexamethasone-induced insulin resistance(IR).Arctostaphylos uva-ursi L.leaves extract and its modified cysteine preparation were obtained according to the developed method with 50%ethanol solution.Their phytochemical profile,hypoglycaemic and pancreatic protective effect were investigated.Phenologlycoside(arbutin),phenolic carboxylic acid(gallic acid),5 flavonoids and 4 hydroxy-cinnamic acids were identified and quantified in the extracts by HPLC.Present data revealed that bearberry leaves alcoholic dry extract enriched with cysteine has a hypoglycaemic and pancreatic protective effect in treated animals under dexamethasone-induced IR model.Treatment improved hyperglycaemia,insulin resistance and beta cell reduc-tion induced by dexamethasone injections.
基金supported by the National Natural Science Foundation of China(32172259,32472381)the Key Research and Development Project of Henan Province(231111111800)the Food Engineering Technology Research Center/Key Laboratory of Henan Province(GO202318).
文摘Selenium-enriched polysaccharides refer to polysaccharides that contain selenium,which have emerged as a significant area of research in recent years due to the bioactivities associated with both polysaccharides and selenium.In this study,we prepared Spirulina platensis polysaccharides(SPP)and selenium-enriched SPP(SeSPP),and assessed their structural properties and hypoglycaemic functions both in vitro and in vivo.The re-sults of the structural characterization indicated that SPP was successfully selenized.The interaction between SPP and selenium primarily occurred in the forms of Se-O-C and O-Se-O through covalent interactions.The molecular weight of SeSPP was found to be more concentrated compared to SPP,and its microscopic morphology exhibited greater complexity,including both rod-shaped and spherical particles.It was determined that SPP and SeSPP were mainly composed of glucose,rhamnose,and fucose.In vitro assays demonstrated that SeSPP exhibited significantly enhanced hypoglycaemic and antioxidant properties compared to SPP.In an in vivo model of streptozotocin-and high-fat diet-induced type 2 diabetes mice,SeSPP improved hypoglycaemic activity by reversing body weight,blood glucose levels,glucose tolerance,insulin resistance,lipid levels,hepatic oxidative stress levels,as well as liver and pancreatic morphology and function.Consequently,SeSPP may serve as a se-lenium supplement that could enhance hypoglycaemic effects.
基金supported by the National Natural Science Foundation of China(32072201,32372286)Natural Science Foundation of Guangdong Province(2023A1515011967,2023A1515012223)+2 种基金Shenzhen Science and Technology Program(JCYJ20230807094503006,JCYJ20230807094503007)Foshan City Science and Technology Tackling Special Project in Social Areas(2120001008478)National Natural Foundation of China(82374239).
文摘Partridge tea is a plant used as a speciality tea drink in Hainan Province,China.The aim of this study was to isolate polyphenols from partridge tea,investigate their main constituent compositions and ameliorative effects on insulin resistance in insulin resistant HepG2(IR-HepG2)cells.PTP was obtained from partridge tea,then PTP-1,PTP-2 and PTP-3 were obtained after further purification.The polyphenol contents of PTP,PTP-1,PTP-2,PTP-3 were determined to be 15.9%,51.4%,42.51% and 22.74%,respectively.PTP and PTP-1 had similar compositions,and the contents of the main components of PTP-1 was higher than that of PTP.Structural compositions and contents of PTP-2 and PTP-3 differ significantly.Further analysis indicated that PTP failed to have significant hypoglycaemic activity,while other three polyphenols regulated the fat metabolism disorders caused by insulin resistance,thus improving the body’s utilization of blood glucose.In addition,the three polyphenols reversed the damage caused by insulin resistance in IR-HepG2 cells by up-regulating the activities of HK(200μg/mL PTP-1:2.8414 mol/min/mL)and PK(200μg/mL PTP-1:10.7204 U/L),increasing the activity of SOD and CAT and decreasing the level of MDA.The mRNA results showed that PTP-1,PTP-2 and PTP-3 could achieve hypoglycaemic efficacy in the body by regulating the IRS-1/PI3K/AKT signal pathway.In summary,our results suggested that the polyphenols of partridge tea could be a promising natural source for preventing and treating hyperglycemia.
