Current animal models of chronic peripheral nerve compression are mainly silicone tube models. However, the cross section of the rat sciatic nerve is not a perfect circle, and there are differences in the diameter of ...Current animal models of chronic peripheral nerve compression are mainly silicone tube models. However, the cross section of the rat sciatic nerve is not a perfect circle, and there are differences in the diameter of the sciatic nerve due to individual differences. The use of a silicone tube with a uniform internal diameter may not provide a reliable and consistent model. We have established a chronic sciatic nerve compression model that can induce demyelination of the sciatic nerve and lead to atrophy of skeletal muscle. In 3-week-old pups and adult rats, the sciatic nerve of the right hind limb was exposed, and a piece of surgical latex glove was gently placed under the nerve. N-butyl-cyanoacrylate was then placed over the nerve, and after it had set, another piece of glove latex was placed on top of the target area and allowed to adhere to the first piece to form a sandwich-like complex. Thus, a chronic sciatic nerve compression model was produced. Control pups with latex or N-butyl-cyanoacrylate were also prepared. Functional changes to nerves were assessed using the hot plate test and electromyography. Immunofluorescence and electron microscopy analyses of the nerves were performed to quantify the degree of neuropathological change. Masson staining was conducted to assess the degree of fibrosis in the gastrocnemius and intrinsic paw muscles. The pup group rats subjected to nerve compression displayed thermal hypoesthesia and a gradual decrease in nerve conduction velocity at 2 weeks after surgery. Neuropathological studies demonstrated that the model caused nerve demyelination and axonal irregularities and triggered collagen deposition in the epineurium and perineurium of the affected nerve at 8 weeks after surgery. The degree of fibrosis in the gastrocnemius and intrinsic paw muscles was significantly increased at 20 weeks after surgery. In conclusion, our novel model can reproduce the functional and histological changes of chronic nerve compression injury that occurs in humans and it will be a useful new tool for investigating the mechanisms underlying chronic nerve compression.展开更多
The intercostobrachial nerve (ICBN) is responsible for the sensory</span></span></span></span></span><span><span><span><span><span><span style="font-...The intercostobrachial nerve (ICBN) is responsible for the sensory</span></span></span></span></span><span><span><span><span><span><span style="font-family:""><span style="font-family:Verdana;"> innervation of a part of the inner side of the arm. Injury of the intercostobrachial nerve is </span><span style="font-family:Verdana;">a complication of axillary dissection during lymph node dissection.</span> <b><span style="font-family:Verdana;">Objective</span></b><span style="font-family:Verdana;">: </span><span style="font-family:Verdana;">This study aimed to determine the effect of preservation of the</span><span style="font-family:Verdana;"> intercostobra</span><span><span style="font-family:Verdana;">chial nerve on postoperative sensory disturbances. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> This is a</span></span><span style="font-family:Verdana;"> prospective, single-center study which was carried out in 90 patients followed in the oncology department of the Aristide Le Dantec hospital in Dakar, suffering </span><span style="font-family:Verdana;">from breast cancer and having undergone breast surgery associated with</span><span style="font-family:Verdana;"> axillary </span><span style="font-family:Verdana;">dissection, for a period of 6 months. The patients were divided into two</span><span style="font-family:Verdana;"> groups depending on whether the intercostobrachial nerve (ICBN) was preserved or </span><span style="font-family:Verdana;">not. The two groups were compared in terms of the incidence of sensory</span><span style="font-family:Verdana;"> disturbances. </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> Sixty patients without nerve preservation and 30 patients with nerve preservation were included in the study, </span><i><span style="font-family:Verdana;">i.e.</span></i><span style="font-family:Verdana;">, 90 patients in total. </span><span style="font-family:Verdana;">ICBN was injured in 60 patients of which 41 patients (83.7%) developed</span><span style="font-family:Verdana;"> numbness in the inner arm. While in the preserved group, only 8 patients suffered from numbness (16.3%) with a significant P value of 0.002;however, for </span><span style="font-family:Verdana;">other </span><span style="font-family:Verdana;">variables such as the incidence of neuropathic pain and hypoesthesia-like</span><span style="font-family:Verdana;"> sensory </span><span><span style="font-family:Verdana;">deficit, there was no significant difference. </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> Preservation of the </span></span><span style="font-family:Verdana;">intercostobrachial nerve during axillary dissection for breast cancer reduces the incidence of sensory disturbances on the upper limb.展开更多
基金supported by the National Natural Science Foundation of China,No.81471270
文摘Current animal models of chronic peripheral nerve compression are mainly silicone tube models. However, the cross section of the rat sciatic nerve is not a perfect circle, and there are differences in the diameter of the sciatic nerve due to individual differences. The use of a silicone tube with a uniform internal diameter may not provide a reliable and consistent model. We have established a chronic sciatic nerve compression model that can induce demyelination of the sciatic nerve and lead to atrophy of skeletal muscle. In 3-week-old pups and adult rats, the sciatic nerve of the right hind limb was exposed, and a piece of surgical latex glove was gently placed under the nerve. N-butyl-cyanoacrylate was then placed over the nerve, and after it had set, another piece of glove latex was placed on top of the target area and allowed to adhere to the first piece to form a sandwich-like complex. Thus, a chronic sciatic nerve compression model was produced. Control pups with latex or N-butyl-cyanoacrylate were also prepared. Functional changes to nerves were assessed using the hot plate test and electromyography. Immunofluorescence and electron microscopy analyses of the nerves were performed to quantify the degree of neuropathological change. Masson staining was conducted to assess the degree of fibrosis in the gastrocnemius and intrinsic paw muscles. The pup group rats subjected to nerve compression displayed thermal hypoesthesia and a gradual decrease in nerve conduction velocity at 2 weeks after surgery. Neuropathological studies demonstrated that the model caused nerve demyelination and axonal irregularities and triggered collagen deposition in the epineurium and perineurium of the affected nerve at 8 weeks after surgery. The degree of fibrosis in the gastrocnemius and intrinsic paw muscles was significantly increased at 20 weeks after surgery. In conclusion, our novel model can reproduce the functional and histological changes of chronic nerve compression injury that occurs in humans and it will be a useful new tool for investigating the mechanisms underlying chronic nerve compression.
文摘The intercostobrachial nerve (ICBN) is responsible for the sensory</span></span></span></span></span><span><span><span><span><span><span style="font-family:""><span style="font-family:Verdana;"> innervation of a part of the inner side of the arm. Injury of the intercostobrachial nerve is </span><span style="font-family:Verdana;">a complication of axillary dissection during lymph node dissection.</span> <b><span style="font-family:Verdana;">Objective</span></b><span style="font-family:Verdana;">: </span><span style="font-family:Verdana;">This study aimed to determine the effect of preservation of the</span><span style="font-family:Verdana;"> intercostobra</span><span><span style="font-family:Verdana;">chial nerve on postoperative sensory disturbances. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> This is a</span></span><span style="font-family:Verdana;"> prospective, single-center study which was carried out in 90 patients followed in the oncology department of the Aristide Le Dantec hospital in Dakar, suffering </span><span style="font-family:Verdana;">from breast cancer and having undergone breast surgery associated with</span><span style="font-family:Verdana;"> axillary </span><span style="font-family:Verdana;">dissection, for a period of 6 months. The patients were divided into two</span><span style="font-family:Verdana;"> groups depending on whether the intercostobrachial nerve (ICBN) was preserved or </span><span style="font-family:Verdana;">not. The two groups were compared in terms of the incidence of sensory</span><span style="font-family:Verdana;"> disturbances. </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> Sixty patients without nerve preservation and 30 patients with nerve preservation were included in the study, </span><i><span style="font-family:Verdana;">i.e.</span></i><span style="font-family:Verdana;">, 90 patients in total. </span><span style="font-family:Verdana;">ICBN was injured in 60 patients of which 41 patients (83.7%) developed</span><span style="font-family:Verdana;"> numbness in the inner arm. While in the preserved group, only 8 patients suffered from numbness (16.3%) with a significant P value of 0.002;however, for </span><span style="font-family:Verdana;">other </span><span style="font-family:Verdana;">variables such as the incidence of neuropathic pain and hypoesthesia-like</span><span style="font-family:Verdana;"> sensory </span><span><span style="font-family:Verdana;">deficit, there was no significant difference. </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> Preservation of the </span></span><span style="font-family:Verdana;">intercostobrachial nerve during axillary dissection for breast cancer reduces the incidence of sensory disturbances on the upper limb.
