Dear Editor,Allele conversion technology has broad application value in plant breeding.Here,we report a user-friendly allele conversion technology based on clustered regularly interspaced short palindromic repeats(CRI...Dear Editor,Allele conversion technology has broad application value in plant breeding.Here,we report a user-friendly allele conversion technology based on clustered regularly interspaced short palindromic repeats(CRISPR)-Cas9 and hyperrecombination lines(HRLs)(Figure 1A).Many agronomically important traits are controlled by single nucleotide polymorphisms(SNPs),insertions or deletions,and other types of small variations between alleles.Traditionally,breeders have relied on backcrossing and selection to introduce a useful allele into the genome of another variety.展开更多
基金supported by the National Natural Science Foundation of China(32272727)the Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-IVFCAAS).
文摘Dear Editor,Allele conversion technology has broad application value in plant breeding.Here,we report a user-friendly allele conversion technology based on clustered regularly interspaced short palindromic repeats(CRISPR)-Cas9 and hyperrecombination lines(HRLs)(Figure 1A).Many agronomically important traits are controlled by single nucleotide polymorphisms(SNPs),insertions or deletions,and other types of small variations between alleles.Traditionally,breeders have relied on backcrossing and selection to introduce a useful allele into the genome of another variety.
