Hyperproliferative keratinocytes and subcutaneous inflammation contribute to the characteristic symptoms of psoriasis,including erythema,scales,or scaly plaques on the skin.These symptoms significantly affect patients...Hyperproliferative keratinocytes and subcutaneous inflammation contribute to the characteristic symptoms of psoriasis,including erythema,scales,or scaly plaques on the skin.These symptoms significantly affect patients'quality of life and cause severe physical and psychological distress.However,current treatment strategies have limited therapeutic effect and may lead to adverse side effects.In this study,we present the novel organic photosensitizer TBTDC[5-(((5-(7-(4-(diphenylamino)phenyl)benzo[c][1,2,5]thiadiazol-4-yl)thiophen-2-yl)methylene)amino)-3-methylthiophene-2,4-dicarbonitrile]nanoparticles(NPs)with aggregation-induced emission(AIE)characteristics to mediate photodynamic therapy(TBTDC NP-PDT)for psoriasis treatment.We demonstrate that TBTDC NPs effectively generate reactive oxygen species upon light irradiation and lead to significant apoptosis of psoriatic keratinocytes.Furthermore,TBTDC NPs exhibit high cellular uptake in diseased keratinocytes and induce endoplasmic reticulum stress(ERS)-mediated autophagy,which can also enhance apoptosis.Importantly,TBTDC NPs show no cytotoxicity toward keratinocytes.These unique properties of TBTDC NPs enable remarkable therapeutic effects against psoriasis-like skin lesions and related inflammation in vivo.Overall,our AIE-active TBTDC NP-PDT represents a promising strategy for treating psoriasis in clinical settings.展开更多
基金supported by the Natural Science Funding of Guangdong Province(2022A1515012312 and 2021A1515010023)the National Natural Science Funding of China(82070083)Dongguan Social development technology projects Science and Technology Planning Project(20231800940792).
文摘Hyperproliferative keratinocytes and subcutaneous inflammation contribute to the characteristic symptoms of psoriasis,including erythema,scales,or scaly plaques on the skin.These symptoms significantly affect patients'quality of life and cause severe physical and psychological distress.However,current treatment strategies have limited therapeutic effect and may lead to adverse side effects.In this study,we present the novel organic photosensitizer TBTDC[5-(((5-(7-(4-(diphenylamino)phenyl)benzo[c][1,2,5]thiadiazol-4-yl)thiophen-2-yl)methylene)amino)-3-methylthiophene-2,4-dicarbonitrile]nanoparticles(NPs)with aggregation-induced emission(AIE)characteristics to mediate photodynamic therapy(TBTDC NP-PDT)for psoriasis treatment.We demonstrate that TBTDC NPs effectively generate reactive oxygen species upon light irradiation and lead to significant apoptosis of psoriatic keratinocytes.Furthermore,TBTDC NPs exhibit high cellular uptake in diseased keratinocytes and induce endoplasmic reticulum stress(ERS)-mediated autophagy,which can also enhance apoptosis.Importantly,TBTDC NPs show no cytotoxicity toward keratinocytes.These unique properties of TBTDC NPs enable remarkable therapeutic effects against psoriasis-like skin lesions and related inflammation in vivo.Overall,our AIE-active TBTDC NP-PDT represents a promising strategy for treating psoriasis in clinical settings.
