Objective To evaluate effect of Neurokinin receptor antagonist on the prevention from hyper-pnea-induced bronchoconstriction using a dual Neurokinin receptor antagonist FK224. Methods 12 pathogen-free Hartley guinea p...Objective To evaluate effect of Neurokinin receptor antagonist on the prevention from hyper-pnea-induced bronchoconstriction using a dual Neurokinin receptor antagonist FK224. Methods 12 pathogen-free Hartley guinea pigs were divided into two groups randomly-. Dimethyl sulfoxide (DMSO) control group (n =6)and FK224 group (n =6). Guinea pigs were anesthetized with pentobarbital sodium. A cervical tracheostomy wasperformed and a polyethylene tube was inserted into the trachea. After measuring baseline value of the lung resist-ance (RL) and dynamic compliance of respiratory system (Cdyn), DMSO (0.3 ml/kg) and FK224 (1mg/kg) wereadministered by injection through jugular vein respectively. A rodent respirator with dry 5% CO2-95% O2 mixture atroom temperature provided mechanical ventilation ( VT 8ml/animal, 100breaths/min) for 5min. RL and Cdyn of 2groups were measured after isocapnic hyperpnea challenge. Results In DMSO control group, isocapnic hyper-pnea of dry gas elicited a marked increase in RL and decrease in Cdyn. RL and Cdyn of FK224 group did not changesignificantly. Conclusion FK224 can inhibit the increase in RL and decrease in Cdyn caused by isocapnic hy-perpnea in guinea pigs. And antagonists of tachykinins receptors might have effect on prophylaxis and treatment inexercise-induced asthma.展开更多
文摘Objective To evaluate effect of Neurokinin receptor antagonist on the prevention from hyper-pnea-induced bronchoconstriction using a dual Neurokinin receptor antagonist FK224. Methods 12 pathogen-free Hartley guinea pigs were divided into two groups randomly-. Dimethyl sulfoxide (DMSO) control group (n =6)and FK224 group (n =6). Guinea pigs were anesthetized with pentobarbital sodium. A cervical tracheostomy wasperformed and a polyethylene tube was inserted into the trachea. After measuring baseline value of the lung resist-ance (RL) and dynamic compliance of respiratory system (Cdyn), DMSO (0.3 ml/kg) and FK224 (1mg/kg) wereadministered by injection through jugular vein respectively. A rodent respirator with dry 5% CO2-95% O2 mixture atroom temperature provided mechanical ventilation ( VT 8ml/animal, 100breaths/min) for 5min. RL and Cdyn of 2groups were measured after isocapnic hyperpnea challenge. Results In DMSO control group, isocapnic hyper-pnea of dry gas elicited a marked increase in RL and decrease in Cdyn. RL and Cdyn of FK224 group did not changesignificantly. Conclusion FK224 can inhibit the increase in RL and decrease in Cdyn caused by isocapnic hy-perpnea in guinea pigs. And antagonists of tachykinins receptors might have effect on prophylaxis and treatment inexercise-induced asthma.
