Objective:To observe the protective effect of fasudil hydrochloride against acute renal injury in septicopyemia rats.Methods:A total of 60 Wister rats were included in the study and divided into control group(n = 10),...Objective:To observe the protective effect of fasudil hydrochloride against acute renal injury in septicopyemia rats.Methods:A total of 60 Wister rats were included in the study and divided into control group(n = 10),model group(n = 25) and treatment group(n = 25).Model group and treatment group received intraperitoneal injection of endotoxin(ET) to establish acute renal injury models while the control group only received daily intraperitoneal injection of normal saline 1 mL.Five rats were taken out of model group and treatment group respectively at 1 h(T1),6 h(T2),12 h(T3),24 h(T4) and 48 h(T5),for intraperitoneal injection of ET 30 mg/kg.Treatment group received intraperitoneal injection of fasudil hydrochloride 30 mg/kg 1 h before injection of ET.For three groups,5 mL blood samples were collected from postcava for determination of serum creatinine and urea nitrogen levels at different time points.Concentrations of serum tumor necrosis factor and ET-1 were determined by using ELISA.The renal pathologic changes were observed under the microscope.Results:Serum creatinine levels in both model group and treatment group were significantly higher than control group at T2-T5(P < 0.05) while the levels in treatment group were significantly lower than control group at T3-T5(P < 0.05).At T2-T5,blood urea nitrogen levels in model group and treatment group were significantly higher than control group(P < 0.05) while the levels in treatment group were significantly lower than model group at T3-T5(P < 0.05).Concentrations of serum tumor necrosis factor in model group and treatment group were significantly higher than control group at T1-T5(P < 0.05) while the levels in treatment group were significantly lower than model group at T1-T5(P < 0.05).Serum ET-1 concentrations in model group and treatment group were significantly higher than control group at T1-T5(P <0.05) while the levels in treatment group at T1-T4 were significantly lower man model group(P <0.05).Rats in control group showed no swelling or hyperaemia in kidney cells but normal structure and normally arranged renal tubular epithelial cells.Obvious injury was observed in model group at T3 and renal tubular epithelial cells in disorder and at swelling condition,hyperaemia and angiectasis in glomerulus,degenerative opacities and vacuolar degeneration,and maximized injury were observed at T4.Injury in renal tissue in treatment group was significantly milder than model group.Conclusions:Fasudil hydrochloride has the significantly protective effect against acute renal injury in septicopyemia rats.展开更多
目的探究盐酸氨溴索纤维支气管镜(简称“纤支镜”)肺泡灌洗(BAL)治疗对肺炎支原体肺炎(MPP)伴气道内黏液栓患儿临床症状、肺实变吸收及支气管肺泡灌洗液(BALF)内黏蛋白5AC(MUC5AC)、磷脂酰肌醇3-激酶(PI3K)与可溶性程序性死亡因子配体-2...目的探究盐酸氨溴索纤维支气管镜(简称“纤支镜”)肺泡灌洗(BAL)治疗对肺炎支原体肺炎(MPP)伴气道内黏液栓患儿临床症状、肺实变吸收及支气管肺泡灌洗液(BALF)内黏蛋白5AC(MUC5AC)、磷脂酰肌醇3-激酶(PI3K)与可溶性程序性死亡因子配体-2(sPD-L2)水平的影响。方法随机数字表法将康复大学青岛中心医院医院2024年6月至2025年10月收治的102例MPP伴气道内黏液栓患儿分为观察组与对照组,每组51例。两组患儿均接受常规综合治疗,此外对照组给予单纯生理盐水纤支镜BAL治疗,观察组则给予盐酸氨溴索混合生理盐水纤支镜BAL治疗。对比两组高热/咳嗽/肺部啰音/黏液栓消失时间、住院时间及肺实变吸收情况;治疗前、治疗10 d后(治疗后)检测对比两组患儿呼吸力学[肺容积(LV)、肺静态顺应性(Cstat)、气道平台压(Pplat)]及血清C反应蛋白(CRP)、白细胞计数(WBC)、白细胞介素-6(IL-6)、干扰素-γ(IFN-γ)水平;首次纤支镜BAL时、末次BAL治疗后第3天采集BALF,测定对比两组BALF内MUC5AC、PI3K、sPD-L2水平。同时统计两组治疗期间不良反应率。结果观察组高热/咳嗽/肺部啰音/黏液栓消失时间、住院时间均短于对照组(P<0.05)。观察组肺实变吸收面积分级优于对照组(P<0.05)。对比治疗前,两组治疗后LV、Cstat均上升(P<0.05),Pplat、血清CRP、WBC、IL-6、IFN-γ水平均下降(P<0.05);对比首次纤支镜BAL时,两组末次BAL治疗后第3 d BALF内MUC5AC、PI3K、sPD-L2水平均下降(P<0.05),且观察组上述指标下降或上升程度均大于对照组(P<0.05)。两组不良反应率(15.69%vs.21.57%)对比差异无统计学意义(P>0.05)。结论盐酸氨溴索纤支镜BAL能有效促进MPP伴气道内黏液栓患儿临床症状体征缓解及肺实变吸收,改善其呼吸力学,其作用机制可能与BALF内MUC5AC、PI3K、sPD-L2水平下调有关。展开更多
DDPH是具有α肾上腺素受体阻断作用和较弱抗钙作用的新化合物。本实验研究了DDPH对麻醉猫、大鼠心肌缺血再灌注所致心律失常的作用和对猫血压的影响。结果表明,DDPH iv 0.5～3 mg/kg可降低麻醉猫血压,减少麻醉猫冠状动脉左前降支结扎时...DDPH是具有α肾上腺素受体阻断作用和较弱抗钙作用的新化合物。本实验研究了DDPH对麻醉猫、大鼠心肌缺血再灌注所致心律失常的作用和对猫血压的影响。结果表明,DDPH iv 0.5～3 mg/kg可降低麻醉猫血压,减少麻醉猫冠状动脉左前降支结扎时心肌缺血产生的VEB,减少麻醉猫和大鼠再灌注所致VEB和VT,VF的持续时间,降低VT,VF的发生率。对动物再灌注时由VF引起的死亡率也有降低的趋势。与哌唑嗪比较,两者具有相似的抗麻醉猫和大鼠心肌缺血再灌注所致心律失常的作用。展开更多
基金Supported by Science and Technology Planning Project of Shandong Province under the fund(No.2014WS012)
文摘Objective:To observe the protective effect of fasudil hydrochloride against acute renal injury in septicopyemia rats.Methods:A total of 60 Wister rats were included in the study and divided into control group(n = 10),model group(n = 25) and treatment group(n = 25).Model group and treatment group received intraperitoneal injection of endotoxin(ET) to establish acute renal injury models while the control group only received daily intraperitoneal injection of normal saline 1 mL.Five rats were taken out of model group and treatment group respectively at 1 h(T1),6 h(T2),12 h(T3),24 h(T4) and 48 h(T5),for intraperitoneal injection of ET 30 mg/kg.Treatment group received intraperitoneal injection of fasudil hydrochloride 30 mg/kg 1 h before injection of ET.For three groups,5 mL blood samples were collected from postcava for determination of serum creatinine and urea nitrogen levels at different time points.Concentrations of serum tumor necrosis factor and ET-1 were determined by using ELISA.The renal pathologic changes were observed under the microscope.Results:Serum creatinine levels in both model group and treatment group were significantly higher than control group at T2-T5(P < 0.05) while the levels in treatment group were significantly lower than control group at T3-T5(P < 0.05).At T2-T5,blood urea nitrogen levels in model group and treatment group were significantly higher than control group(P < 0.05) while the levels in treatment group were significantly lower than model group at T3-T5(P < 0.05).Concentrations of serum tumor necrosis factor in model group and treatment group were significantly higher than control group at T1-T5(P < 0.05) while the levels in treatment group were significantly lower than model group at T1-T5(P < 0.05).Serum ET-1 concentrations in model group and treatment group were significantly higher than control group at T1-T5(P <0.05) while the levels in treatment group at T1-T4 were significantly lower man model group(P <0.05).Rats in control group showed no swelling or hyperaemia in kidney cells but normal structure and normally arranged renal tubular epithelial cells.Obvious injury was observed in model group at T3 and renal tubular epithelial cells in disorder and at swelling condition,hyperaemia and angiectasis in glomerulus,degenerative opacities and vacuolar degeneration,and maximized injury were observed at T4.Injury in renal tissue in treatment group was significantly milder than model group.Conclusions:Fasudil hydrochloride has the significantly protective effect against acute renal injury in septicopyemia rats.
文摘目的探究盐酸氨溴索纤维支气管镜(简称“纤支镜”)肺泡灌洗(BAL)治疗对肺炎支原体肺炎(MPP)伴气道内黏液栓患儿临床症状、肺实变吸收及支气管肺泡灌洗液(BALF)内黏蛋白5AC(MUC5AC)、磷脂酰肌醇3-激酶(PI3K)与可溶性程序性死亡因子配体-2(sPD-L2)水平的影响。方法随机数字表法将康复大学青岛中心医院医院2024年6月至2025年10月收治的102例MPP伴气道内黏液栓患儿分为观察组与对照组,每组51例。两组患儿均接受常规综合治疗,此外对照组给予单纯生理盐水纤支镜BAL治疗,观察组则给予盐酸氨溴索混合生理盐水纤支镜BAL治疗。对比两组高热/咳嗽/肺部啰音/黏液栓消失时间、住院时间及肺实变吸收情况;治疗前、治疗10 d后(治疗后)检测对比两组患儿呼吸力学[肺容积(LV)、肺静态顺应性(Cstat)、气道平台压(Pplat)]及血清C反应蛋白(CRP)、白细胞计数(WBC)、白细胞介素-6(IL-6)、干扰素-γ(IFN-γ)水平;首次纤支镜BAL时、末次BAL治疗后第3天采集BALF,测定对比两组BALF内MUC5AC、PI3K、sPD-L2水平。同时统计两组治疗期间不良反应率。结果观察组高热/咳嗽/肺部啰音/黏液栓消失时间、住院时间均短于对照组(P<0.05)。观察组肺实变吸收面积分级优于对照组(P<0.05)。对比治疗前,两组治疗后LV、Cstat均上升(P<0.05),Pplat、血清CRP、WBC、IL-6、IFN-γ水平均下降(P<0.05);对比首次纤支镜BAL时,两组末次BAL治疗后第3 d BALF内MUC5AC、PI3K、sPD-L2水平均下降(P<0.05),且观察组上述指标下降或上升程度均大于对照组(P<0.05)。两组不良反应率(15.69%vs.21.57%)对比差异无统计学意义(P>0.05)。结论盐酸氨溴索纤支镜BAL能有效促进MPP伴气道内黏液栓患儿临床症状体征缓解及肺实变吸收,改善其呼吸力学,其作用机制可能与BALF内MUC5AC、PI3K、sPD-L2水平下调有关。
文摘DDPH是具有α肾上腺素受体阻断作用和较弱抗钙作用的新化合物。本实验研究了DDPH对麻醉猫、大鼠心肌缺血再灌注所致心律失常的作用和对猫血压的影响。结果表明,DDPH iv 0.5～3 mg/kg可降低麻醉猫血压,减少麻醉猫冠状动脉左前降支结扎时心肌缺血产生的VEB,减少麻醉猫和大鼠再灌注所致VEB和VT,VF的持续时间,降低VT,VF的发生率。对动物再灌注时由VF引起的死亡率也有降低的趋势。与哌唑嗪比较,两者具有相似的抗麻醉猫和大鼠心肌缺血再灌注所致心律失常的作用。
