研究毛竹叶柄多糖(polysaccharide from moso bamboo leaves,简称PMBL)的体外抗氧化作用。以芦丁和VC为对照,测定PMBL对二苯代苦味酰自由基(DPPH)、超氧阴离子自由基(O2-·)和羟自由基(·OH)的清除能力,同时用硫代巴比妥酸(TBA...研究毛竹叶柄多糖(polysaccharide from moso bamboo leaves,简称PMBL)的体外抗氧化作用。以芦丁和VC为对照,测定PMBL对二苯代苦味酰自由基(DPPH)、超氧阴离子自由基(O2-·)和羟自由基(·OH)的清除能力,同时用硫代巴比妥酸(TBA)法测定其对亚油酸过氧化的抑制作用。结果表明,PMBL对O2-·和·OH均具有较强清除作用,其IC50分别为5.98mg/L和5.55mg/L,效果优于VC和芦丁;对DPPH(IC50=36.08mg/L)的清除能力略弱于VC和芦丁;TBA实验表明PMBL抗脂质过氧化效果不够理想。展开更多
为了研究玉米蛋白粉的酶解关键技术和利用电子束辐照技术辅助提高玉米蛋白粉的酶解效果,该研究以蛋白质质量分数为79.4%的玉米蛋白粉为原料,以水解度(degree of hydrolysis,DH)和1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picryl-hydrazy...为了研究玉米蛋白粉的酶解关键技术和利用电子束辐照技术辅助提高玉米蛋白粉的酶解效果,该研究以蛋白质质量分数为79.4%的玉米蛋白粉为原料,以水解度(degree of hydrolysis,DH)和1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picryl-hydrazyl,DPPH)清除率为衡量指标,借助单因素和响应面试验设计方法,在优选碱性蛋白酶可控酶解制备玉米抗氧化肽的最佳工艺参数基础上,研究了不同辐照剂量的电子束辐照处理对玉米蛋白粉酶解效果的影响,并利用场发射扫描电镜检测技术分析电子束辐照前后玉米蛋白粉表面形态的变化。研究发现:当碱性蛋白酶的酶解温度为50℃、酶解p H值为8.6、酶与底物浓度比为9.13%时,玉米蛋白粉的水解度可达40.26%±0.23%;所制备的玉米抗氧化肽的DPPH清除率为70.74%±0.41%;若采用辐照剂量为3.24 k Gy进行电子束辐照处理玉米蛋白粉时,则其水解度可达到49.17%±0.42%,其DPPH清除率可达73.03%±1.02%;经场发射扫描电镜检测分析得知,被辐照处理后的玉米蛋白粉表面凹凸程度增加,完整性下降。研究结果可为玉米精深加工产业和玉米抗氧化肽的深入研究提供理论参考。展开更多
研究药桑椹花青素(anthocyanins from Morus nigra L.,AMNL)的体外抗氧化作用,并与白桑椹花青素(anthocyanins from Morus alba L.,AMAL)和黑桑椹花青素(anthocyanins from Morus alba Linn.var.tatarica,AMALV)的体外抗氧化作用进行比...研究药桑椹花青素(anthocyanins from Morus nigra L.,AMNL)的体外抗氧化作用,并与白桑椹花青素(anthocyanins from Morus alba L.,AMAL)和黑桑椹花青素(anthocyanins from Morus alba Linn.var.tatarica,AMALV)的体外抗氧化作用进行比较。以芦丁和VC为对照,测定AMNL对二苯代苦味酰肼自由基(DPPH自由基)、超氧阴离子自由基(O2.)、羟自由基(.OH)和烷基自由基的清除能力,同时测定其体外总抗氧化能力。结果表明,AMNL对DPPH自由基的清除能力(IC50=9.07mg/L)显著高于AMAL与AMALV,弱于芦丁和VC。AMNL对O2.(IC50=2.82mg/L)和.OH(IC50=7.77mg/L)的清除能力不仅明显高于AMAL与AMALV,而且清除效果也优于芦丁和VC。各桑椹花青素对烷基自由基清除率均低于50%。AMNL总抗氧化能力分别是AMAL、AMALV及芦丁的11.8倍、2.6倍及1.3倍,但低于VC。展开更多
Food provides not only essential nutrients required for life, but also bioactive compounds usefull to maintan good health and disease prevention. Abundant epidemiological evidences suggest that consumption of food ric...Food provides not only essential nutrients required for life, but also bioactive compounds usefull to maintan good health and disease prevention. Abundant epidemiological evidences suggest that consumption of food rich in antioxi-dants (non-nutritional components) can prevent degenerative diseases. A total of 26 commonly consumed nuts, oil seeds, edible oils, milk and milk products were chosen for the study. Considering the fact that antioxidant content (AOC) and phenolic contents (PC) of these foods was not established systematically in Indian context. Therefore, we have assessed and correlated the AOC and PC, an important antioxidant constituents of plant foods. AOC was assessed by DPPH (2, 2-Diphenyl-1-picryl hydrazyl) scavenging activity and FRAP (Ferric reducing antioxidant power) methods and phenolic content (PC), using Folin-Ciocalteu reagent. Among the nuts and oil seeds arecanut had the highest phenolic and antioxidant content 10841, 4220341 mg/100g respectively. In milk, edible oils and sugars the values ranged from 336 -11674 mg/100g. Jaggery had the highest PC and AOC among the foods studied . Although AOC and PC showed wide variation among the foods, AOC was correlated significantly with PC. Indeed the ‘r’ value between PC and AOC (DPPH and FRAP) was 0.99 (p < 0.01) among the nuts and oil seeds, while in milk, milk products and sugars, the “r” values ranged from 0.93 and 0.99 (p < 0.01) respectively. The overall results indicate that the phenolic compounds may be significant contributors to the AOC of the foods studied.展开更多
This work was undertaken to explore the potential of fruit waste materials as sources of natural antioxidants. Kernels of four varieties of mangoes of Indian origin were studied. Kernels were extracted with five diffe...This work was undertaken to explore the potential of fruit waste materials as sources of natural antioxidants. Kernels of four varieties of mangoes of Indian origin were studied. Kernels were extracted with five different solvents, tested for extraction efficiency and total phenolic content （TPC）. As the methanol proved to be the best solvent, it was used for further analysis. The methanolic extracts of kernels had reducing power （RP） and scavenged 2, 2 diphenyl-l-picryl hydrazyl （DPPH） radical, which is related to their antioxidant activity （AA）. When analyzed in HPLC, the extracts showed the presence of phenolic compounds. The extracts had the higher capacity to reduce the formation of peroxides and slow down the rate of oxidation than butylated hydroxyl toluene （BHT） in refined, bleached deodorized soybean oil （RBD SBO）. Based on the results obtained mango kernels are potential source of natural antioxidants owing to their antioxidant activity.展开更多
Five batches from the same stock of seeds of Ocimum basilicum were irradiated at 5, 10, 15, 20 and 25 Gy, respectively using <sup>60</sup>C source. Methanolic leaf extracts of these samples and a control w...Five batches from the same stock of seeds of Ocimum basilicum were irradiated at 5, 10, 15, 20 and 25 Gy, respectively using <sup>60</sup>C source. Methanolic leaf extracts of these samples and a control were evaluated for their antioxidant activity by the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging method using M<sub>2</sub> plants. All the methanolic extracts showed antioxidant activity. The IC<sub>50</sub> of the methanolic extracts of the six different treatments, control, 5 Gy, 10 Gy, 15 Gy, 20 Gy and 25 Gy, showed antioxidant activity with IC<sub>50</sub> values of 100, 90, 86, 61, 71 and 70 μg/ml, respectively. Three individual mutants, M-15-5, M-20-6 and M-15-4, had IC<sub>50</sub> values of 26, 30 and 40 μg/ml, respectively. These mutants were from the 15 Gy and 20 Gy treatments. From the results, it is confirmed that induced mutation can be employed to create variation in the levels of free radical scavenging activity in O. basilicum and can therefore serve as a tool for breeding for high levels of antioxidant activity in O. basilicum.展开更多
The antioxidant of seeds was carried out using extracts from methanol and Silver Nanoparticles from the spice. The SEM shows the shapes, dispersion and agglomeration of the sample, while the EDX confirms the SEM and t...The antioxidant of seeds was carried out using extracts from methanol and Silver Nanoparticles from the spice. The SEM shows the shapes, dispersion and agglomeration of the sample, while the EDX confirms the SEM and the presence of some compounds. The FT-IR reveals the AgNP<sub>s</sub> capping and reducing the particular biomolecule from the functional group for identification. Compounds found in the FT-IR seeds of Capsicum annum are Ag L (Silver iodide), C K (Cyanogen chloride), P K (Phenol). Monodora myristica are Mo L (Molybdenum), Ag L (Silver iodide), C K (Cyanogen chloride), P K (Phenol), Mg K (Magnesium). Piper guineense are Ag L (Silver iodide), Ci K (Potassium chloride), C K (Cyanogen chloride), P K (Phenol). The seeds show that the AgNP<sub>S</sub> of CA and MM has a better antioxidant activity than the methanol of CA and MM, while the PG methanol has a better activity than the AgNP<sub>S</sub> PG. The control (Catechin and Galic acid) has a slight overall better DPPH activity than the AgNP<sub>S</sub>. It is important to note that there is a concentration dependency in CA, MM AgNP<sub>S,</sub> PG methanol respectively. Notably, at CA methanol, the conc. at 125 was higher than the conc. at 250. Hence, there is need to create a great part in using plant samples for making tabulated or capsulated drugs for treatment of diseases and using plant silver nanoparticles to develop a healthy food/drug preservative package material “smart packaging” that will enhance shelf-life.展开更多
文摘研究毛竹叶柄多糖(polysaccharide from moso bamboo leaves,简称PMBL)的体外抗氧化作用。以芦丁和VC为对照,测定PMBL对二苯代苦味酰自由基(DPPH)、超氧阴离子自由基(O2-·)和羟自由基(·OH)的清除能力,同时用硫代巴比妥酸(TBA)法测定其对亚油酸过氧化的抑制作用。结果表明,PMBL对O2-·和·OH均具有较强清除作用,其IC50分别为5.98mg/L和5.55mg/L,效果优于VC和芦丁;对DPPH(IC50=36.08mg/L)的清除能力略弱于VC和芦丁;TBA实验表明PMBL抗脂质过氧化效果不够理想。
文摘为了研究玉米蛋白粉的酶解关键技术和利用电子束辐照技术辅助提高玉米蛋白粉的酶解效果,该研究以蛋白质质量分数为79.4%的玉米蛋白粉为原料,以水解度(degree of hydrolysis,DH)和1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picryl-hydrazyl,DPPH)清除率为衡量指标,借助单因素和响应面试验设计方法,在优选碱性蛋白酶可控酶解制备玉米抗氧化肽的最佳工艺参数基础上,研究了不同辐照剂量的电子束辐照处理对玉米蛋白粉酶解效果的影响,并利用场发射扫描电镜检测技术分析电子束辐照前后玉米蛋白粉表面形态的变化。研究发现:当碱性蛋白酶的酶解温度为50℃、酶解p H值为8.6、酶与底物浓度比为9.13%时,玉米蛋白粉的水解度可达40.26%±0.23%;所制备的玉米抗氧化肽的DPPH清除率为70.74%±0.41%;若采用辐照剂量为3.24 k Gy进行电子束辐照处理玉米蛋白粉时,则其水解度可达到49.17%±0.42%,其DPPH清除率可达73.03%±1.02%;经场发射扫描电镜检测分析得知,被辐照处理后的玉米蛋白粉表面凹凸程度增加,完整性下降。研究结果可为玉米精深加工产业和玉米抗氧化肽的深入研究提供理论参考。
文摘研究药桑椹花青素(anthocyanins from Morus nigra L.,AMNL)的体外抗氧化作用,并与白桑椹花青素(anthocyanins from Morus alba L.,AMAL)和黑桑椹花青素(anthocyanins from Morus alba Linn.var.tatarica,AMALV)的体外抗氧化作用进行比较。以芦丁和VC为对照,测定AMNL对二苯代苦味酰肼自由基(DPPH自由基)、超氧阴离子自由基(O2.)、羟自由基(.OH)和烷基自由基的清除能力,同时测定其体外总抗氧化能力。结果表明,AMNL对DPPH自由基的清除能力(IC50=9.07mg/L)显著高于AMAL与AMALV,弱于芦丁和VC。AMNL对O2.(IC50=2.82mg/L)和.OH(IC50=7.77mg/L)的清除能力不仅明显高于AMAL与AMALV,而且清除效果也优于芦丁和VC。各桑椹花青素对烷基自由基清除率均低于50%。AMNL总抗氧化能力分别是AMAL、AMALV及芦丁的11.8倍、2.6倍及1.3倍,但低于VC。
文摘Food provides not only essential nutrients required for life, but also bioactive compounds usefull to maintan good health and disease prevention. Abundant epidemiological evidences suggest that consumption of food rich in antioxi-dants (non-nutritional components) can prevent degenerative diseases. A total of 26 commonly consumed nuts, oil seeds, edible oils, milk and milk products were chosen for the study. Considering the fact that antioxidant content (AOC) and phenolic contents (PC) of these foods was not established systematically in Indian context. Therefore, we have assessed and correlated the AOC and PC, an important antioxidant constituents of plant foods. AOC was assessed by DPPH (2, 2-Diphenyl-1-picryl hydrazyl) scavenging activity and FRAP (Ferric reducing antioxidant power) methods and phenolic content (PC), using Folin-Ciocalteu reagent. Among the nuts and oil seeds arecanut had the highest phenolic and antioxidant content 10841, 4220341 mg/100g respectively. In milk, edible oils and sugars the values ranged from 336 -11674 mg/100g. Jaggery had the highest PC and AOC among the foods studied . Although AOC and PC showed wide variation among the foods, AOC was correlated significantly with PC. Indeed the ‘r’ value between PC and AOC (DPPH and FRAP) was 0.99 (p < 0.01) among the nuts and oil seeds, while in milk, milk products and sugars, the “r” values ranged from 0.93 and 0.99 (p < 0.01) respectively. The overall results indicate that the phenolic compounds may be significant contributors to the AOC of the foods studied.
文摘This work was undertaken to explore the potential of fruit waste materials as sources of natural antioxidants. Kernels of four varieties of mangoes of Indian origin were studied. Kernels were extracted with five different solvents, tested for extraction efficiency and total phenolic content （TPC）. As the methanol proved to be the best solvent, it was used for further analysis. The methanolic extracts of kernels had reducing power （RP） and scavenged 2, 2 diphenyl-l-picryl hydrazyl （DPPH） radical, which is related to their antioxidant activity （AA）. When analyzed in HPLC, the extracts showed the presence of phenolic compounds. The extracts had the higher capacity to reduce the formation of peroxides and slow down the rate of oxidation than butylated hydroxyl toluene （BHT） in refined, bleached deodorized soybean oil （RBD SBO）. Based on the results obtained mango kernels are potential source of natural antioxidants owing to their antioxidant activity.
文摘Five batches from the same stock of seeds of Ocimum basilicum were irradiated at 5, 10, 15, 20 and 25 Gy, respectively using <sup>60</sup>C source. Methanolic leaf extracts of these samples and a control were evaluated for their antioxidant activity by the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging method using M<sub>2</sub> plants. All the methanolic extracts showed antioxidant activity. The IC<sub>50</sub> of the methanolic extracts of the six different treatments, control, 5 Gy, 10 Gy, 15 Gy, 20 Gy and 25 Gy, showed antioxidant activity with IC<sub>50</sub> values of 100, 90, 86, 61, 71 and 70 μg/ml, respectively. Three individual mutants, M-15-5, M-20-6 and M-15-4, had IC<sub>50</sub> values of 26, 30 and 40 μg/ml, respectively. These mutants were from the 15 Gy and 20 Gy treatments. From the results, it is confirmed that induced mutation can be employed to create variation in the levels of free radical scavenging activity in O. basilicum and can therefore serve as a tool for breeding for high levels of antioxidant activity in O. basilicum.
文摘The antioxidant of seeds was carried out using extracts from methanol and Silver Nanoparticles from the spice. The SEM shows the shapes, dispersion and agglomeration of the sample, while the EDX confirms the SEM and the presence of some compounds. The FT-IR reveals the AgNP<sub>s</sub> capping and reducing the particular biomolecule from the functional group for identification. Compounds found in the FT-IR seeds of Capsicum annum are Ag L (Silver iodide), C K (Cyanogen chloride), P K (Phenol). Monodora myristica are Mo L (Molybdenum), Ag L (Silver iodide), C K (Cyanogen chloride), P K (Phenol), Mg K (Magnesium). Piper guineense are Ag L (Silver iodide), Ci K (Potassium chloride), C K (Cyanogen chloride), P K (Phenol). The seeds show that the AgNP<sub>S</sub> of CA and MM has a better antioxidant activity than the methanol of CA and MM, while the PG methanol has a better activity than the AgNP<sub>S</sub> PG. The control (Catechin and Galic acid) has a slight overall better DPPH activity than the AgNP<sub>S</sub>. It is important to note that there is a concentration dependency in CA, MM AgNP<sub>S,</sub> PG methanol respectively. Notably, at CA methanol, the conc. at 125 was higher than the conc. at 250. Hence, there is need to create a great part in using plant samples for making tabulated or capsulated drugs for treatment of diseases and using plant silver nanoparticles to develop a healthy food/drug preservative package material “smart packaging” that will enhance shelf-life.
