BACKGROUND Human-derived gastric cancer organoids(GCOs)are widely used in gastric cancer research;however,the culture success rate is generally low.AIM To explore the potential influencing factors,and the literature o...BACKGROUND Human-derived gastric cancer organoids(GCOs)are widely used in gastric cancer research;however,the culture success rate is generally low.AIM To explore the potential influencing factors,and the literature on successful culture rates of GCOs was reviewed using meta-analysis.METHODS PubMed,Web of Science,and EMBASE were searched for studies.Two trained researchers selected the studies and extracted data.STATA 17.0 software was used for meta-analysis of the incidence of each outcome event.The adjusted Methodological Index for Non-Randomized Studies scale was used to assess the quality of the included studies.Funnel plots and Egger’s test were used to detect publication bias.Subgroup analyses were conducted for sex,tissue source,histo-logical classification,and the pathological tumor-node-metastasis(pTNM)cancer staging system.RESULTS Eight studies with a pooled success rate of 66.6%were included.GCOs derived from women and men had success rates of 67%and 46.7%,respectively.GCOs from surgery or biopsy/endoscopic submucosal dissection showed success rates of 70.9%and 53.7%,respectively.GCOs of poorly-differentiated,moderately-differentiated and signet-ring cell cancer showed success rates of 64.6%,31%,and 32.7%,respectively.GCOs with pTNM stages I-II and III-IV showed success rates of 38.3%and 65.2%,respectively.Y-27632 and non-Y-27632 use showed success rates of 58.2%and 70%,respectively.GCOs generated with collagenase were more successful than those constructed with Liberase TH and TrypLE(72.1%vs 71%,respectively).EDTA digestion showed a 50%lower success rate than other methods(P=0.04).CONCLUSION GCO establishment rate is low and varies by sex,tissue source,histological type,and pTNM stage.Omitting Y-27632,and using Liberase TH,TrypLE,or collagenase yields greater success than EDTA.展开更多
Exploring the natural availability and intrinsic bioactivity of blood-derived proteins opens new avenues for fabricating bioactive and patient-specific solutions for biomedical applications.Despite their several advan...Exploring the natural availability and intrinsic bioactivity of blood-derived proteins opens new avenues for fabricating bioactive and patient-specific solutions for biomedical applications.Despite their several advantages,their use as inks for 3D printing is limited due to suboptimal rheological properties.To address this,we propose a dual-step strategy based on the initial generation of blood protein-based bulk hydrogels encompassing pristine and photo-responsive protein mixtures to allow their mechanical granularization followed by jamming,establishing injectable and printable granular inks.In this study,two globular-based protein matrices-human platelet lysates(PL)and bovine serum albumin(BSA)-were used as granular inks for 3D printing.We hypothesize that nozzle jamming-in contrast to the typically employed centrifugal jamming-would render optimized results for the granular protein inks’processability.Printability was evaluated in filaments,scaffold grids,and convoluted structures.Taking advantage of the previously introduced photocurable moieties,post-printing photocrosslinking was used for the annealing of themicrogels,leading to increased scaffold mechanical stability and robustness.The nozzle jamming methodology imparted the best print performance and reproducibility,where PLMA-based inks outperformed the BSAMA-based.In addition,the microgel granular constructs allowed primary human-derived adipose stem cells to adhere and proliferate,whereas the PLMA-based ink demonstrated higher cell affinity and enhanced biological performance.We further demonstrated that bioinks could be developed from PLMA-based inks,showcasing high viability without compromising 3D printing performance.Overall,this study gives clear insights into the importance of the jamming process as well as the granularization outcome requirements for the obtention of highly reproducible granular inks for 3D printing.展开更多
Vascular endothelial growth factor （VEGF） is an angiogenic regulator that stimulates endothelial cell migration, proliferation, and angiogenesis. VEGF gene therapy is a new promising approach to induce therapeutic a...Vascular endothelial growth factor （VEGF） is an angiogenic regulator that stimulates endothelial cell migration, proliferation, and angiogenesis. VEGF gene therapy is a new promising approach to induce therapeutic angiogenesis for the treatment of ischemic myocardial and limb diseases. Recently, clinical studies have demonstrated successful outcomes using plasmids, retroviruses and adenoviruses.l-3 But the safety of those vectors is poor, which has become a serious concern. Besides immunogenicity caused by viral vectors, a further problem is that viral vectors show a preference to integrate into the transcription or control region of active genes, which may induce tumors and other disorders.展开更多
基金Supported by National Natural Science Foundation of China,No.82174309 and No.81973774National Administration of Traditional Chinese Medicine:2019 Project of Building Evidence-Based Practice Capacity for TCM,No.2019XZZX-XH013Shuguang Hospital Siming Foundation Research Special Project,No.SGKJ-202304.
文摘BACKGROUND Human-derived gastric cancer organoids(GCOs)are widely used in gastric cancer research;however,the culture success rate is generally low.AIM To explore the potential influencing factors,and the literature on successful culture rates of GCOs was reviewed using meta-analysis.METHODS PubMed,Web of Science,and EMBASE were searched for studies.Two trained researchers selected the studies and extracted data.STATA 17.0 software was used for meta-analysis of the incidence of each outcome event.The adjusted Methodological Index for Non-Randomized Studies scale was used to assess the quality of the included studies.Funnel plots and Egger’s test were used to detect publication bias.Subgroup analyses were conducted for sex,tissue source,histo-logical classification,and the pathological tumor-node-metastasis(pTNM)cancer staging system.RESULTS Eight studies with a pooled success rate of 66.6%were included.GCOs derived from women and men had success rates of 67%and 46.7%,respectively.GCOs from surgery or biopsy/endoscopic submucosal dissection showed success rates of 70.9%and 53.7%,respectively.GCOs of poorly-differentiated,moderately-differentiated and signet-ring cell cancer showed success rates of 64.6%,31%,and 32.7%,respectively.GCOs with pTNM stages I-II and III-IV showed success rates of 38.3%and 65.2%,respectively.Y-27632 and non-Y-27632 use showed success rates of 58.2%and 70%,respectively.GCOs generated with collagenase were more successful than those constructed with Liberase TH and TrypLE(72.1%vs 71%,respectively).EDTA digestion showed a 50%lower success rate than other methods(P=0.04).CONCLUSION GCO establishment rate is low and varies by sex,tissue source,histological type,and pTNM stage.Omitting Y-27632,and using Liberase TH,TrypLE,or collagenase yields greater success than EDTA.
基金supported by the European Union(EU)Horizon 2020 for the project InterLynk(Grant agreement:953169)Funda玢o para a Ciência e Tecnologia(10.54499/2022.04605.CEECIND/CP1720/CT0021,BI/UI89/10303/2022,PTDC/BTM-MAT/3201/2020,and PRT/BD/154735/2023)+3 种基金FCT/MEC(PIDDAC)Säo Paulo Research Foundation(FAPESP)grants#2018/12871-0,#2021/10844-8,and#2013/07296-2(CEPID)National Council for Scientific and Technological Development(CNPq)Coordination of Superior Level Staff Improvement(CAPES)Finance Code 001.
文摘Exploring the natural availability and intrinsic bioactivity of blood-derived proteins opens new avenues for fabricating bioactive and patient-specific solutions for biomedical applications.Despite their several advantages,their use as inks for 3D printing is limited due to suboptimal rheological properties.To address this,we propose a dual-step strategy based on the initial generation of blood protein-based bulk hydrogels encompassing pristine and photo-responsive protein mixtures to allow their mechanical granularization followed by jamming,establishing injectable and printable granular inks.In this study,two globular-based protein matrices-human platelet lysates(PL)and bovine serum albumin(BSA)-were used as granular inks for 3D printing.We hypothesize that nozzle jamming-in contrast to the typically employed centrifugal jamming-would render optimized results for the granular protein inks’processability.Printability was evaluated in filaments,scaffold grids,and convoluted structures.Taking advantage of the previously introduced photocurable moieties,post-printing photocrosslinking was used for the annealing of themicrogels,leading to increased scaffold mechanical stability and robustness.The nozzle jamming methodology imparted the best print performance and reproducibility,where PLMA-based inks outperformed the BSAMA-based.In addition,the microgel granular constructs allowed primary human-derived adipose stem cells to adhere and proliferate,whereas the PLMA-based ink demonstrated higher cell affinity and enhanced biological performance.We further demonstrated that bioinks could be developed from PLMA-based inks,showcasing high viability without compromising 3D printing performance.Overall,this study gives clear insights into the importance of the jamming process as well as the granularization outcome requirements for the obtention of highly reproducible granular inks for 3D printing.
基金a grant from the National Natural Science Foundation of China(No.30270734)
文摘Vascular endothelial growth factor （VEGF） is an angiogenic regulator that stimulates endothelial cell migration, proliferation, and angiogenesis. VEGF gene therapy is a new promising approach to induce therapeutic angiogenesis for the treatment of ischemic myocardial and limb diseases. Recently, clinical studies have demonstrated successful outcomes using plasmids, retroviruses and adenoviruses.l-3 But the safety of those vectors is poor, which has become a serious concern. Besides immunogenicity caused by viral vectors, a further problem is that viral vectors show a preference to integrate into the transcription or control region of active genes, which may induce tumors and other disorders.
