Hydrogen has emerged as a promising clean energy source,leading to numerous recent efforts to integrate hydrogen into turbine engine applications[1].This integration has the potential to significantly enhance engine e...Hydrogen has emerged as a promising clean energy source,leading to numerous recent efforts to integrate hydrogen into turbine engine applications[1].This integration has the potential to significantly enhance engine efficiency while reducing carbon dioxide emissions[2].However,the degradation of nickel alloys induced by hydrogen has been well documented[3-7].Consequently,hydrogen-assisted failure of nickel alloys poses a critical concern for the design and safe operation of hydrogen-powered turbine engines.展开更多
High-performance lithium metal batteries benefit from the construction of composite polymer electrolytes(CPEs)which are synthesized by incorporating inorganic fillers into polymer matrices[1].However,the random distri...High-performance lithium metal batteries benefit from the construction of composite polymer electrolytes(CPEs)which are synthesized by incorporating inorganic fillers into polymer matrices[1].However,the random distribution of added fillers within the polymer matrix can lead to tortuous ion pathways and longer transmission distances(Fig.1).As a result,the ion transport capability of CPEs may decrease,while interface contact may deteriorate.Therefore,the organized arrangement of fillers emerges as a crucial consideration in constructing electrolyte membranes.One highly effective approach is the adoption of a vertically aligned filler configuration,where ceramic fillers are constructed to be perpendicular to the electrolyte membrane.If so,the filler/electrolyte interface impedance can be significantly reduced,while continuous ion transport channels along the specified direction are formed,thus significantly enhancing the ion conduction(Fig.1(a))[1].展开更多
The unique wave-manipulation capabilities of zero-index metamaterials(ZIMs)offer a new opportunity for realizing bound states in the continuum(BICs).However,the relationship between anomalous scattering and BICs remai...The unique wave-manipulation capabilities of zero-index metamaterials(ZIMs)offer a new opportunity for realizing bound states in the continuum(BICs).However,the relationship between anomalous scattering and BICs remains underexplored when parity–time(PT)symmetry is introduced.In this work,we demonstrate that a BIC splits into a pair of lasing modes carrying opposite topological charges by introducing PT symmetry through gain-loss cylinders embedded in ZIM layers.Theoretical analysis and numerical simulations reveal that lasing and unidirectional transparency phenomena result from the singularities and exceptional points of the scattering matrix.Moreover,exceptional points can be tuned via propagation phase modulation in the air gap,and their coalescence produces quasi-BICs with symmetric responses.This work provides a framework for manipulating BICs and topological lasing modes in non-Hermitian systems,offering new insights for designing non-Hermitian photonic devices.展开更多
To the Editor:Chinese herbal medicines(CHMs)are critical components of traditional Chinese medicine(TCM),[1]with their cold and hot properties being fundamental for guiding clinical applications and ensuring therapeut...To the Editor:Chinese herbal medicines(CHMs)are critical components of traditional Chinese medicine(TCM),[1]with their cold and hot properties being fundamental for guiding clinical applications and ensuring therapeutic efficacy.[2]However,the traditional method of identifying the cold and hot properties of CHMs relies heavily on the subjective experience of TCM practitioners and clinical practice,often leading to uncertainty and inconsistency.As such,there is a pressing need for more precise,rapid,and objective strategies to accurately identify the cold and hot properties of CHMs and their active ingredients.展开更多
Chiral metal-organic frameworks(CMOFs),an emerging class of heterogeneous chiral catalysts,show significant potential for application in asymmetric catalysis due to their tunable pore shapes and sizes,large internal s...Chiral metal-organic frameworks(CMOFs),an emerging class of heterogeneous chiral catalysts,show significant potential for application in asymmetric catalysis due to their tunable pore shapes and sizes,large internal surface areas,and rich chiral environments[1-4].Through over 20 years of development,researchers have invested heavily in exploring new CMOFs to enhance asymmetric catalytic performance[5-10].However,the dual pursuit of a substantial specific surface area and robust stability within a single CMOF presents a considerable challenge.Additionally,efficient CMOF catalysts,frequently synthesized from privileged chiral ligands such as proline,2,2'-bis(diphenylphosphino)-1,1'-binaph thyl(BINAP),Salen,and bis(oxazoline)(Box)[11,12],tend to exhibit more complex structures compared to their corresponding privileged chiral counterparts,thereby necessitating higher production costs.Consequently,the development of novel synthetic methodologies for constructing CMOFs as effective chiral catalysts remains a challenging endeavor.展开更多
Flexible hybrid systems usually combine soft modules(mechanically matched with skin or clothes)and hard modulus(like rigid circuits).However,the risk of interface failure due to modulus mismatch between flexible compo...Flexible hybrid systems usually combine soft modules(mechanically matched with skin or clothes)and hard modulus(like rigid circuits).However,the risk of interface failure due to modulus mismatch between flexible components and rigid circuits limits the system’s complexity and durability.The diverse features of flexible components further complicate the development of a universal interface.In this work,we demonstrated a cocoon-mimetic feature-matched interface(CFI)that offers stable electrical contact with flexible surface.It also matches flexible systems features in stretchability(lower than 0.22Ωcm^(-1)during 900%elongation),durability(stable resistance after 20000 times 100%elongation),breathability(gas permeability 614 mm S^(-1))and self-adhesive(0.18±0.01 N mm^(-1)).We developed a direct spray-on-skin sensor and used CFI to form a hand task recognition system.This system,deployable in seconds,has 97.7%accuracy in recognition of eight hand tasks.This research offers a promising solution for flexible hybrid systems interfacing challenges.展开更多
Slot-die coating with halogen-free solvents is a promising scalable fabrication strategy for organic solar cells(OSCs).However,the complex interplay between long-time-scale solute diffusion and microstructural evoluti...Slot-die coating with halogen-free solvents is a promising scalable fabrication strategy for organic solar cells(OSCs).However,the complex interplay between long-time-scale solute diffusion and microstructural evolution during the coating process remains poorly understood,limiting further optimization of morphology and device performance.In this study,we elucidate the critical role of solution viscosity in regulating phase separation and aggregation kinetics.Specifically,lower solution viscosity enhances solute diffusion,accelerating molecular aggregation while suppressing liquid-liquid phase separation(LLPS).Notably,we observe that in three different systems with varying crystallinity and immiscibility(PM6:Y6,PTQ10:Y6,and D18:Y6),the optimal processing conditions for peak device efficiency consistently correspond to a nearly identical solution viscosity(∼0.8 mPa s),despite variations in optimal processing temperatures.In situ characterizations reveal that at this viscosity,all three systems exhibit constrained LLPS and rapid molecular aggregation,promoting the formation of finely structured,continuous nanoscale domains.These findings establish solution viscosity as a universal governing parameter for morphology control in printed active layers.By providing a fundamental framework for understanding viscosity-mediated phase separation,this work offers valuable insights for advancing high-throughput,environmentally friendly printing techniques for high-efficiency OSCs.展开更多
Transient electronics,comprising of degradable devices that disintegrate and disappear after their operational life,has received considerable interest in recent years because of the concerns related to the rapidly gro...Transient electronics,comprising of degradable devices that disintegrate and disappear after their operational life,has received considerable interest in recent years because of the concerns related to the rapidly growing electronic waste(e-waste).However,the degradability or biodegradability of electronic devices alone is insufficient to ascertain environmental safety.The evaluation of the nature of degradation by-products is also essential to assess the environmental impact of a degradable device.Herein,we investigate systematically the hydrolytic degradation by-products of two different types of devices viz.a capacitive pressure sensor and a photodetector,using liquid chromatograph mass spectrometry.The findings reveal that,despite the inherent degradability of constituent materials used in an electronic device,the released by-products can be toxic or could be complex molecules with unknown chemistry such as carcinogenic or contain almost non-degradable polystyrene derivatives/microplastic(e.g.,from PEDOT:PSS),or have copper complexes resulting from degraded silk fibroin and poly(ethylene oxide)mass fragments.This analysis underlines the need for careful selection and reassessment of materials employed in transient electronics,as an important factor,to mitigate the end-of-life issues associated with electronics and its environmental impact.展开更多
Trophoblast stem cells(TSCs)are placental progenitors derived from the trophectoderm or the extraembryonic ectoderm.Recently,human TSCs were established via a specific culture system[1].However,the process by which hu...Trophoblast stem cells(TSCs)are placental progenitors derived from the trophectoderm or the extraembryonic ectoderm.Recently,human TSCs were established via a specific culture system[1].However,the process by which human TSCs further differentiate into terminal trophoblasts that mimic placental development remains obscure and interesting.Hence,the development of a suitable celltool to robustly study the mechanism underlying the self-renewal or differentiation of human TSCs is urgently needed.Mammalian haploid cells resemble their diploid counterparts but have a single genome,which is widely used in the genetic screening of many biological processes.However,no haploid cells for an extraembryonic lineage in humans have been derived.展开更多
To the Editor:Pathogenic copy number variants(CNVs)have been associated with congenital anomalies and neurodevelopmental disorders.[1]However,some fetuses with pathogenic CNVs have structural abnormalities,whereas oth...To the Editor:Pathogenic copy number variants(CNVs)have been associated with congenital anomalies and neurodevelopmental disorders.[1]However,some fetuses with pathogenic CNVs have structural abnormalities,whereas others appear phenotypically normal.Prenatal assessment of CNVs in fetuses with structural abnormalities currently relies on invasive prenatal diagnosis(IPD).However,the absence of routine screening for CNVs in pregnant women whose fetuses do not have structural abnormalities restricts the ability to identify clinically significant fetal CNVs.展开更多
Exosomes(Exos)are emerging as noninvasive biomarkers for diagnosis and progression monitoring of gastric cancer(GC).However,the heterogeneity discrimination and ultrasensitive quantification of Exos presents a conside...Exosomes(Exos)are emerging as noninvasive biomarkers for diagnosis and progression monitoring of gastric cancer(GC).However,the heterogeneity discrimination and ultrasensitive quantification of Exos presents a considerable analytical challenge,thereby impeding severely their clinical application.Herein,we propose an integrated terahertz metasensing platform for the discrimination of Exos in distinct subtypes of GC in a single step—through the simultaneous evaluation of the category and richness level of Exos membrane proteins.Characterized by dual-sided independent sensing capabilities with enhanced sensitivity(169 and 325 GHz per refractive index unit,respectively),the metasensor functionalized with antibodies simultaneously reflects the content of 2 membrane proteins in the terahertz spectral response.Our approach concurrently completes accurate differentiation and precise quantification of GC-subtype Exos by integrating dual-sided sensing information in merely a single assay.The dual-sided sensing design enhances the reliability of detection results.Moreover,combined with the signal amplification of gold nanoparticles,the platform experimentally demonstrates a superior dynamic response to Exos concentrations spanning from 1×10^(4)to 1×10^(8)particles/ml,with the limit of detection being 1×10^(4)particles/ml.This work provides new insights into multisensing metasurface design and paves the way for precise and personalized cancer treatment through the specific sensing of Exos.展开更多
The relentless pursuit of high-energy-density batteries has reignited global in-terest in lithium metal batteries(LMBs).However,the commercialization of LMBs has faced insurmountable hurdles during their initial explo...The relentless pursuit of high-energy-density batteries has reignited global in-terest in lithium metal batteries(LMBs).However,the commercialization of LMBs has faced insurmountable hurdles during their initial exploration,primarily due to uncontrolled lithium dendrite growth,electrolyte decomposition,and catastrophic safety risks such as thermal runaway.These challenges led to the abandonment of lithium metal anodes in favor of graphite-based systems,which dominated the market due to their inherent stability,albeit at the cost of significantly lower energy density.展开更多
Evidence-based practice address to use the best available evidence to support clinical practice.The evidence can be diverse according to the evidence hierarchy model ranging from case report and expert experience up t...Evidence-based practice address to use the best available evidence to support clinical practice.The evidence can be diverse according to the evidence hierarchy model ranging from case report and expert experience up to randomized controlled trials(RCTs).However,the hierarchy of evidence in modern medicine is undergoing a fundamental transformation.While RCTs have long occupied the apex of the evidence pyramid,their idealized designs often fail to address complex clinical questions.Other types of evidence such as case series,case-control study,and cohort study can be part of decision-making aid.The US FDA's 2016 Real-World Evidence Program marked a pivotal moment,formally recognizing real-world evidence(RWE)as valid supplementary evidence for regulatory decisions.Nowadays,people are thinking how clinical evidence could be adopted to refl ect the real-world practice.In this context,real world study was generated.展开更多
The Realgar-Indigo Naturalis formula(RIF)is a proprietary Chinese medicine,which is one of the important drugs in the treatment of pediatric acute promyelocytic leukemia(APL).However,the dose of RIF in clinical applic...The Realgar-Indigo Naturalis formula(RIF)is a proprietary Chinese medicine,which is one of the important drugs in the treatment of pediatric acute promyelocytic leukemia(APL).However,the dose of RIF in clinical application is not uniform and the long-term effectiveness and safety of combining RIF with all-trans retinoic acid(ATRA)in a larger population of pediatric APL patients remains undocumented.We conducted a multicenter single-arm clinical trial(ChiCTR-OIC-16010014)in China.Individuals newly diagnosed with APL were treated with CCCG-APL-2017 protocol which is based on RIF and ATRA in consolidation.The event-free survival(EFS)and overall survival(OS)outcomes were evaluated.We recruited 200 patients diagnosed with APL.The six-year OS rate was 100%in the low-risk(LR)group and 97.6%in the high-risk(HR)group.The six-year EFS rate was 98.3%in the LR group and 97.6%in the HR group.Plasma levels of arsenic remained stable after the administration of RIF at a dosage of 60 mg/kg/d for seven days and returned to baseline levels within fourteen days after discontinuation of RIF administration,which is consistent with a concentration of 135 mg/d/kg.Furthermore,controlling white blood cells(WBC)to maintain levels at or below 30×10^(9)/L during induction therapy can decrease the incidence of induced differentiation syndrome(DS)or alleviate its symptoms.Our study demonstrated that the CCCG-APL-2017 protocol,which combines RIF with ATRA,is both effective and safe in treating children with APL.展开更多
Introduction:Vaccination stands as the most effective preventive measure against yellow fever(YF).However,the YF vaccination associated Adverse Events following Immunization(AEFI)cases occur occasionally.Methods:The G...Introduction:Vaccination stands as the most effective preventive measure against yellow fever(YF).However,the YF vaccination associated Adverse Events following Immunization(AEFI)cases occur occasionally.Methods:The Guangdong Provincial Center for Disease Control and Prevention utilized Targeted Next-Generation Sequencing(tNGS)to determine whether the imported suspected YF case was infected by the wild-type YF virus strain or experienced an AEFI.Results:tNGS analysis successfully yielded a 10.2 kb viral genomic sequence.Subsequent in-depth analysis revealed high similarity to the YF vaccine strain 17D-213 and classified the sequence within the West Africa II genotype,clustering with the 17D vaccine strain.Conclusions and Implications for Public Health Practice:This case represented YF AEFI.The implementation of tNGS technology enables more precise and expeditious pathogen sequencing,providing critical evidence for accurate disease diagnosis and informed public health interventions.展开更多
Emerging evidence suggests that priming intestinal stem cells(ISCs)towards secretory progenitor cells is beneficial for maintaining gut homeostasis against inflammatory bowel disease(IBD).However,the mechanism driving...Emerging evidence suggests that priming intestinal stem cells(ISCs)towards secretory progenitor cells is beneficial for maintaining gut homeostasis against inflammatory bowel disease(IBD).However,the mechanism driving such biased lineage commitment remains elusive.Here we show that MG53,also named as TRIM72,prompts ISCs to secretory lineages via upregulating peroxisome proliferator-activated receptorα(PPARα),thus maintaining intestinal epithelium integrity against noxious insults.Using genetic mouse models,we found that MG53 deficiency leads to exacerbated intestinal damage caused by various injuries in mice,whereas MG53 overexpression in ISCs is sufficient to ameliorate such damage.Mechanistically,MG53 promoted asymmetric division of ISCs to generate more progenitor cells of secretory lineages via activating PPARαsignaling.Specifically,MG53 overexpression induced PPARαexpression at transcriptional level and concomitantly increased PPARαactivity by elevating the contents of a panel of unsaturated fatty acids in the intestine that serve as potent endogenous agonists of PPARα.Furthermore,genetic ablation or pharmacological inhibition of PPARαabolished the protective effects of MG53.These findings reveal a crucial role of MG53-PPARαaxis in driving the secretory lineage commitment of ISCs,especially during injury response,highlighting the important therapeutic potential of targeting MG53-PPARαsignaling for IBD treatment and marking PPARαagonists as novel therapies for IBD caused by various etiologies.展开更多
基金supported by the Science Center for Gas Turbine Project(No.P2022-B-IV-009-002).
文摘Hydrogen has emerged as a promising clean energy source,leading to numerous recent efforts to integrate hydrogen into turbine engine applications[1].This integration has the potential to significantly enhance engine efficiency while reducing carbon dioxide emissions[2].However,the degradation of nickel alloys induced by hydrogen has been well documented[3-7].Consequently,hydrogen-assisted failure of nickel alloys poses a critical concern for the design and safe operation of hydrogen-powered turbine engines.
基金supported by the National Natural Science Foundation of China(No.51972293)Hangzhou Key Research Program Project(2023SZD0099)LingYan Project(2024C01090).
文摘High-performance lithium metal batteries benefit from the construction of composite polymer electrolytes(CPEs)which are synthesized by incorporating inorganic fillers into polymer matrices[1].However,the random distribution of added fillers within the polymer matrix can lead to tortuous ion pathways and longer transmission distances(Fig.1).As a result,the ion transport capability of CPEs may decrease,while interface contact may deteriorate.Therefore,the organized arrangement of fillers emerges as a crucial consideration in constructing electrolyte membranes.One highly effective approach is the adoption of a vertically aligned filler configuration,where ceramic fillers are constructed to be perpendicular to the electrolyte membrane.If so,the filler/electrolyte interface impedance can be significantly reduced,while continuous ion transport channels along the specified direction are formed,thus significantly enhancing the ion conduction(Fig.1(a))[1].
基金supported by the National Natural Science Foundation of China(Grant Nos.12504361,12274313,and 62465005)the Natural Science Foundation of Guangxi(Grant No.2025GXNSFBA069179)the Guangxi Colleges and Universities Young and Middle-aged Teachers’Basic Scientific Research Ability Enhancement Project(Grant No.2025KY0093)。
文摘The unique wave-manipulation capabilities of zero-index metamaterials(ZIMs)offer a new opportunity for realizing bound states in the continuum(BICs).However,the relationship between anomalous scattering and BICs remains underexplored when parity–time(PT)symmetry is introduced.In this work,we demonstrate that a BIC splits into a pair of lasing modes carrying opposite topological charges by introducing PT symmetry through gain-loss cylinders embedded in ZIM layers.Theoretical analysis and numerical simulations reveal that lasing and unidirectional transparency phenomena result from the singularities and exceptional points of the scattering matrix.Moreover,exceptional points can be tuned via propagation phase modulation in the air gap,and their coalescence produces quasi-BICs with symmetric responses.This work provides a framework for manipulating BICs and topological lasing modes in non-Hermitian systems,offering new insights for designing non-Hermitian photonic devices.
基金supported by the Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(CACMS)(No.CI2023C065YLL)Scientific and technological innovation project of CACMS(No.CI2021B003)+1 种基金CACMS Innovation Fund(No.CI2021A01509)National Natural Science Foundation of China(No.61872297).
文摘To the Editor:Chinese herbal medicines(CHMs)are critical components of traditional Chinese medicine(TCM),[1]with their cold and hot properties being fundamental for guiding clinical applications and ensuring therapeutic efficacy.[2]However,the traditional method of identifying the cold and hot properties of CHMs relies heavily on the subjective experience of TCM practitioners and clinical practice,often leading to uncertainty and inconsistency.As such,there is a pressing need for more precise,rapid,and objective strategies to accurately identify the cold and hot properties of CHMs and their active ingredients.
基金supported by the National Key R&D Program of China(2022YFA1503300)and the National Natural Science Foundation of China(21971241 and 21935010)。
文摘Chiral metal-organic frameworks(CMOFs),an emerging class of heterogeneous chiral catalysts,show significant potential for application in asymmetric catalysis due to their tunable pore shapes and sizes,large internal surface areas,and rich chiral environments[1-4].Through over 20 years of development,researchers have invested heavily in exploring new CMOFs to enhance asymmetric catalytic performance[5-10].However,the dual pursuit of a substantial specific surface area and robust stability within a single CMOF presents a considerable challenge.Additionally,efficient CMOF catalysts,frequently synthesized from privileged chiral ligands such as proline,2,2'-bis(diphenylphosphino)-1,1'-binaph thyl(BINAP),Salen,and bis(oxazoline)(Box)[11,12],tend to exhibit more complex structures compared to their corresponding privileged chiral counterparts,thereby necessitating higher production costs.Consequently,the development of novel synthetic methodologies for constructing CMOFs as effective chiral catalysts remains a challenging endeavor.
基金supported by the National Key Research and Development Program of China(2021YFC3002200)the National Natural Science Foundation of China(U20A20168)+1 种基金the Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(CI2023C002YG,ZN2023A01)a grant from the Guoqiang Institute,Tsinghua University.
文摘Flexible hybrid systems usually combine soft modules(mechanically matched with skin or clothes)and hard modulus(like rigid circuits).However,the risk of interface failure due to modulus mismatch between flexible components and rigid circuits limits the system’s complexity and durability.The diverse features of flexible components further complicate the development of a universal interface.In this work,we demonstrated a cocoon-mimetic feature-matched interface(CFI)that offers stable electrical contact with flexible surface.It also matches flexible systems features in stretchability(lower than 0.22Ωcm^(-1)during 900%elongation),durability(stable resistance after 20000 times 100%elongation),breathability(gas permeability 614 mm S^(-1))and self-adhesive(0.18±0.01 N mm^(-1)).We developed a direct spray-on-skin sensor and used CFI to form a hand task recognition system.This system,deployable in seconds,has 97.7%accuracy in recognition of eight hand tasks.This research offers a promising solution for flexible hybrid systems interfacing challenges.
基金the National Natural Science Foundation of China(W2411049,52303247,and 52173023)the Fundamental Research Funds for the Central Universities(xzy012023169)+2 种基金the Postdoctoral Research Project of Shaanxi Province(2023BSHEDZZ24)the 111 Project 2.0(BP0618008)the China Postdoctoral Science Foundation(2023TQ0273)。
文摘Slot-die coating with halogen-free solvents is a promising scalable fabrication strategy for organic solar cells(OSCs).However,the complex interplay between long-time-scale solute diffusion and microstructural evolution during the coating process remains poorly understood,limiting further optimization of morphology and device performance.In this study,we elucidate the critical role of solution viscosity in regulating phase separation and aggregation kinetics.Specifically,lower solution viscosity enhances solute diffusion,accelerating molecular aggregation while suppressing liquid-liquid phase separation(LLPS).Notably,we observe that in three different systems with varying crystallinity and immiscibility(PM6:Y6,PTQ10:Y6,and D18:Y6),the optimal processing conditions for peak device efficiency consistently correspond to a nearly identical solution viscosity(∼0.8 mPa s),despite variations in optimal processing temperatures.In situ characterizations reveal that at this viscosity,all three systems exhibit constrained LLPS and rapid molecular aggregation,promoting the formation of finely structured,continuous nanoscale domains.These findings establish solution viscosity as a universal governing parameter for morphology control in printed active layers.By providing a fundamental framework for understanding viscosity-mediated phase separation,this work offers valuable insights for advancing high-throughput,environmentally friendly printing techniques for high-efficiency OSCs.
文摘Transient electronics,comprising of degradable devices that disintegrate and disappear after their operational life,has received considerable interest in recent years because of the concerns related to the rapidly growing electronic waste(e-waste).However,the degradability or biodegradability of electronic devices alone is insufficient to ascertain environmental safety.The evaluation of the nature of degradation by-products is also essential to assess the environmental impact of a degradable device.Herein,we investigate systematically the hydrolytic degradation by-products of two different types of devices viz.a capacitive pressure sensor and a photodetector,using liquid chromatograph mass spectrometry.The findings reveal that,despite the inherent degradability of constituent materials used in an electronic device,the released by-products can be toxic or could be complex molecules with unknown chemistry such as carcinogenic or contain almost non-degradable polystyrene derivatives/microplastic(e.g.,from PEDOT:PSS),or have copper complexes resulting from degraded silk fibroin and poly(ethylene oxide)mass fragments.This analysis underlines the need for careful selection and reassessment of materials employed in transient electronics,as an important factor,to mitigate the end-of-life issues associated with electronics and its environmental impact.
基金supported by the National Key Research and Development Program of China(2023YFC2705900)the Fundamental Research Funds for the Central Universities(63223030)+1 种基金the National Natural Science Foundation of China(82371671,82201840,and 82301879)the Natural Science Foundation of Tianjin City(22JCZDJC00480).
文摘Trophoblast stem cells(TSCs)are placental progenitors derived from the trophectoderm or the extraembryonic ectoderm.Recently,human TSCs were established via a specific culture system[1].However,the process by which human TSCs further differentiate into terminal trophoblasts that mimic placental development remains obscure and interesting.Hence,the development of a suitable celltool to robustly study the mechanism underlying the self-renewal or differentiation of human TSCs is urgently needed.Mammalian haploid cells resemble their diploid counterparts but have a single genome,which is widely used in the genetic screening of many biological processes.However,no haploid cells for an extraembryonic lineage in humans have been derived.
基金supported by grants from the National Key R&D Program of China(Nos.2022YFC2703400 and 2023YFC2705605)the Key R&D Program of Sichuan Province(No.2024YFFK0266).
文摘To the Editor:Pathogenic copy number variants(CNVs)have been associated with congenital anomalies and neurodevelopmental disorders.[1]However,some fetuses with pathogenic CNVs have structural abnormalities,whereas others appear phenotypically normal.Prenatal assessment of CNVs in fetuses with structural abnormalities currently relies on invasive prenatal diagnosis(IPD).However,the absence of routine screening for CNVs in pregnant women whose fetuses do not have structural abnormalities restricts the ability to identify clinically significant fetal CNVs.
基金supported by the Young Scientists Fund of the National Natural Science Foundation of China(Grant Nos.62401629 and 12304354).
文摘Exosomes(Exos)are emerging as noninvasive biomarkers for diagnosis and progression monitoring of gastric cancer(GC).However,the heterogeneity discrimination and ultrasensitive quantification of Exos presents a considerable analytical challenge,thereby impeding severely their clinical application.Herein,we propose an integrated terahertz metasensing platform for the discrimination of Exos in distinct subtypes of GC in a single step—through the simultaneous evaluation of the category and richness level of Exos membrane proteins.Characterized by dual-sided independent sensing capabilities with enhanced sensitivity(169 and 325 GHz per refractive index unit,respectively),the metasensor functionalized with antibodies simultaneously reflects the content of 2 membrane proteins in the terahertz spectral response.Our approach concurrently completes accurate differentiation and precise quantification of GC-subtype Exos by integrating dual-sided sensing information in merely a single assay.The dual-sided sensing design enhances the reliability of detection results.Moreover,combined with the signal amplification of gold nanoparticles,the platform experimentally demonstrates a superior dynamic response to Exos concentrations spanning from 1×10^(4)to 1×10^(8)particles/ml,with the limit of detection being 1×10^(4)particles/ml.This work provides new insights into multisensing metasurface design and paves the way for precise and personalized cancer treatment through the specific sensing of Exos.
基金supported by the Science and Technology Research and Development Joint Foundation of Henan Province under grant number 242301420003the Postdoctoral Fellowship Program of CPSF under grant number GZB20240683.
文摘The relentless pursuit of high-energy-density batteries has reignited global in-terest in lithium metal batteries(LMBs).However,the commercialization of LMBs has faced insurmountable hurdles during their initial exploration,primarily due to uncontrolled lithium dendrite growth,electrolyte decomposition,and catastrophic safety risks such as thermal runaway.These challenges led to the abandonment of lithium metal anodes in favor of graphite-based systems,which dominated the market due to their inherent stability,albeit at the cost of significantly lower energy density.
文摘Evidence-based practice address to use the best available evidence to support clinical practice.The evidence can be diverse according to the evidence hierarchy model ranging from case report and expert experience up to randomized controlled trials(RCTs).However,the hierarchy of evidence in modern medicine is undergoing a fundamental transformation.While RCTs have long occupied the apex of the evidence pyramid,their idealized designs often fail to address complex clinical questions.Other types of evidence such as case series,case-control study,and cohort study can be part of decision-making aid.The US FDA's 2016 Real-World Evidence Program marked a pivotal moment,formally recognizing real-world evidence(RWE)as valid supplementary evidence for regulatory decisions.Nowadays,people are thinking how clinical evidence could be adopted to refl ect the real-world practice.In this context,real world study was generated.
基金supported by National Key Research and Development Program of China(2021YFE0106900)the Chinese Academy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(2021-I2M-1-041,2021-I2M-1-001)the National Natural Science Foundation of China(82270144).
文摘The Realgar-Indigo Naturalis formula(RIF)is a proprietary Chinese medicine,which is one of the important drugs in the treatment of pediatric acute promyelocytic leukemia(APL).However,the dose of RIF in clinical application is not uniform and the long-term effectiveness and safety of combining RIF with all-trans retinoic acid(ATRA)in a larger population of pediatric APL patients remains undocumented.We conducted a multicenter single-arm clinical trial(ChiCTR-OIC-16010014)in China.Individuals newly diagnosed with APL were treated with CCCG-APL-2017 protocol which is based on RIF and ATRA in consolidation.The event-free survival(EFS)and overall survival(OS)outcomes were evaluated.We recruited 200 patients diagnosed with APL.The six-year OS rate was 100%in the low-risk(LR)group and 97.6%in the high-risk(HR)group.The six-year EFS rate was 98.3%in the LR group and 97.6%in the HR group.Plasma levels of arsenic remained stable after the administration of RIF at a dosage of 60 mg/kg/d for seven days and returned to baseline levels within fourteen days after discontinuation of RIF administration,which is consistent with a concentration of 135 mg/d/kg.Furthermore,controlling white blood cells(WBC)to maintain levels at or below 30×10^(9)/L during induction therapy can decrease the incidence of induced differentiation syndrome(DS)or alleviate its symptoms.Our study demonstrated that the CCCG-APL-2017 protocol,which combines RIF with ATRA,is both effective and safe in treating children with APL.
基金Supported by the Guangdong Provincial Key Laboratory of Pathogen Detection for Emerging Infectious Disease Response(2023B1212010010).
文摘Introduction:Vaccination stands as the most effective preventive measure against yellow fever(YF).However,the YF vaccination associated Adverse Events following Immunization(AEFI)cases occur occasionally.Methods:The Guangdong Provincial Center for Disease Control and Prevention utilized Targeted Next-Generation Sequencing(tNGS)to determine whether the imported suspected YF case was infected by the wild-type YF virus strain or experienced an AEFI.Results:tNGS analysis successfully yielded a 10.2 kb viral genomic sequence.Subsequent in-depth analysis revealed high similarity to the YF vaccine strain 17D-213 and classified the sequence within the West Africa II genotype,clustering with the 17D vaccine strain.Conclusions and Implications for Public Health Practice:This case represented YF AEFI.The implementation of tNGS technology enables more precise and expeditious pathogen sequencing,providing critical evidence for accurate disease diagnosis and informed public health interventions.
基金funded by National Key R&D Program of China(2022YFA1303003,2018YFA0800701,2018YFA0507603,and 2018YFA0800501)National Natural Science Foundation of China(81770376,81630008,81790621,31521062,31671177,and 81370234).
文摘Emerging evidence suggests that priming intestinal stem cells(ISCs)towards secretory progenitor cells is beneficial for maintaining gut homeostasis against inflammatory bowel disease(IBD).However,the mechanism driving such biased lineage commitment remains elusive.Here we show that MG53,also named as TRIM72,prompts ISCs to secretory lineages via upregulating peroxisome proliferator-activated receptorα(PPARα),thus maintaining intestinal epithelium integrity against noxious insults.Using genetic mouse models,we found that MG53 deficiency leads to exacerbated intestinal damage caused by various injuries in mice,whereas MG53 overexpression in ISCs is sufficient to ameliorate such damage.Mechanistically,MG53 promoted asymmetric division of ISCs to generate more progenitor cells of secretory lineages via activating PPARαsignaling.Specifically,MG53 overexpression induced PPARαexpression at transcriptional level and concomitantly increased PPARαactivity by elevating the contents of a panel of unsaturated fatty acids in the intestine that serve as potent endogenous agonists of PPARα.Furthermore,genetic ablation or pharmacological inhibition of PPARαabolished the protective effects of MG53.These findings reveal a crucial role of MG53-PPARαaxis in driving the secretory lineage commitment of ISCs,especially during injury response,highlighting the important therapeutic potential of targeting MG53-PPARαsignaling for IBD treatment and marking PPARαagonists as novel therapies for IBD caused by various etiologies.
