We first introduce the empirical mode decomposition （EMD） and holo-spectrum analysis. Then we decompose the vibration signal of the equipment by EMD, re-organize the signal closely associated with the original signa...We first introduce the empirical mode decomposition （EMD） and holo-spectrum analysis. Then we decompose the vibration signal of the equipment by EMD, re-organize the signal closely associated with the original signal, eliminate the noise signal better, and analyze the holographic re-signal spectrum, which makes the amplitude, frequency and phase combinecompletely, reflecting the equipment vibration morphology fully. Particularly applying a two-dimensional holo-spectrum can obtain the direction of rotation order harmonic, size, shape, and the relation- . ship between order harmonic generation rotor vibrations, and so on. The traditional signal processing methods can not eliminate noise well. We apply this method to the rotor vibration signal of fault diagnosis, eliminate noise and reflect the equipment vibration characteristics well.展开更多
Cup-like nuclear morphological alterations in acute myeloid leukemia(AML)blasts have been widely correlated with Nucleophosmin 1(NPM1)mutations.NPM1-mutated AML has earned recognition as a distinct entity among myeloi...Cup-like nuclear morphological alterations in acute myeloid leukemia(AML)blasts have been widely correlated with Nucleophosmin 1(NPM1)mutations.NPM1-mutated AML has earned recognition as a distinct entity among myeloid tumors,but the absence of a thoroughly established tool for its morphological analysis remains a notable gap.Holographic tomography(HT)can offer a label-free solution for quantitatively assessing the 3D shape of the nucleus based on the volumetric variations of its refractive indices(RIs).However,traditional HT methods analyze adherent cells in a 2D layer,leading to non-isotropic reconstructions due to missing cone artifacts.Here we show for the first time that holo-tomographic flow cytometry(HTFC)achieves quantitative specificity and precise capture of the nucleus volumetric shape in AML cells in suspension.To retrieve nucleus specificity in label-free RI tomograms of flowing AML cells,we conceive and demonstrate in a real-world clinical case a novel strategy for segmenting 3D concave nuclei.This method implies that the correlation between the"phenotype"and"genotype"of nuclei is demonstrated through HTFC by creating a challenging link not yet explored between the aberrant morphological features of AML nuclei and NPM1 mutations.We conduct an ensemble-level statistical characterization of NPM1-wild type and NPM1-mutated blasts to discern their complex morphological and biophysical variances.Our findings suggest that characterizing cup-like nuclei in NPM1-related AML cells by HTFC may enhance the diagnostic approach for these tumors.Furthermore,we integrate virtual reality to provide an immersive fruition of morphological changes in AML cells within a true 3D environment.展开更多
Holo-tomographic flow cytometry for label-free phenotyping of suspended acute myeloid leukemia blasts is demonstrated.A concave segmentation algorithm is applied to 3D refractive index tomograms to quantify NPM1-mutat...Holo-tomographic flow cytometry for label-free phenotyping of suspended acute myeloid leukemia blasts is demonstrated.A concave segmentation algorithm is applied to 3D refractive index tomograms to quantify NPM1-mutation-associated cup-like nuclear morphologies,with virtual reality visualization offering engaging immersion.The method enables population-level detection of statistically significant shifts in 3D cell morphology,originally correlating phenotype with genotype.展开更多
Targeted drugs could significantly reduce cytotoxic effect and increase therapeutic activity. Dihydroartemisinin (DHA) has been shown to be effective in killing cancer cells. However, it exhibits non-targeted property...Targeted drugs could significantly reduce cytotoxic effect and increase therapeutic activity. Dihydroartemisinin (DHA) has been shown to be effective in killing cancer cells. However, it exhibits non-targeted property. Holo-transferrin (TF) is a suitable drug-carrier to target cancer cells, because cancer cells need iron uptake by the TF-mediated mechanism to maintain their uncontrolled growth. Furthermore, TF receptors (TF-R) are highly expressed on cancer cell surfaces. In this paper, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the different killing effect of 4-(12-Dihydroartemisininoxy) Benzoic Acid Hydrozide-transferrin (DBAH-TF) on human breast cancer cells (MCF-7) cells and human normal breast (HNB) cells, and atomic force microscopy (AFM) was used to visually observe the targeted effect of DBAH-TF on MCF-7 cells. MTT results show that DBAH-TF is 172 times more potent than DHA in killing MCF-7 cells, while the cytotoxic effect of DBAH-TF on HNB cells is merely 1/33 to DHA. Also, the killing effect of DBAH-TF on MCF-7 cells is 286 times that on HNB cells, showing targeted effect. Moreover, there are distinct differences in ultrastructures of cellular surfaces after DBAH-TF and DHA treatment. Through AFM imaging, many characteristic holes were observed on the cancer cell surface after being effected by DBAH-TF, which differ from the holes with irregular shapes affected by DHA. These results visually show that the DBAH-TF targeted drug has more potent killing effect on MCF-7 cells compared with DHA.展开更多
文摘We first introduce the empirical mode decomposition （EMD） and holo-spectrum analysis. Then we decompose the vibration signal of the equipment by EMD, re-organize the signal closely associated with the original signal, eliminate the noise signal better, and analyze the holographic re-signal spectrum, which makes the amplitude, frequency and phase combinecompletely, reflecting the equipment vibration morphology fully. Particularly applying a two-dimensional holo-spectrum can obtain the direction of rotation order harmonic, size, shape, and the relation- . ship between order harmonic generation rotor vibrations, and so on. The traditional signal processing methods can not eliminate noise well. We apply this method to the rotor vibration signal of fault diagnosis, eliminate noise and reflect the equipment vibration characteristics well.
基金supported by the project PRIN 2022 PNRR—flow-cytometry ImaGing by Holographic tomography for predicting TUMor control in Oncology patients treated with Radiotherapy(FIGHT-TUMOR),Prot.P2022ATE2J—funded by the Italian Ministry of University&Research in the framework of Next Generation EUby project“CITOM”—Programma AMICO 2,CNR—UVR—within the PoC 2022—PNRR funded by the Italian Ministry of Business and Made in Italy—UIBM in the framework of Next Generation EUby#NEXTGENERATIONEU(NGEU),Ministry of University and Research(MUR),National Recovery and Resilience Plan(NRRP),project MNESYS(PE0000006)—a multiscale integrated approach to the study of the nervous system in health and disease(DN.155311.10.2022)。
文摘Cup-like nuclear morphological alterations in acute myeloid leukemia(AML)blasts have been widely correlated with Nucleophosmin 1(NPM1)mutations.NPM1-mutated AML has earned recognition as a distinct entity among myeloid tumors,but the absence of a thoroughly established tool for its morphological analysis remains a notable gap.Holographic tomography(HT)can offer a label-free solution for quantitatively assessing the 3D shape of the nucleus based on the volumetric variations of its refractive indices(RIs).However,traditional HT methods analyze adherent cells in a 2D layer,leading to non-isotropic reconstructions due to missing cone artifacts.Here we show for the first time that holo-tomographic flow cytometry(HTFC)achieves quantitative specificity and precise capture of the nucleus volumetric shape in AML cells in suspension.To retrieve nucleus specificity in label-free RI tomograms of flowing AML cells,we conceive and demonstrate in a real-world clinical case a novel strategy for segmenting 3D concave nuclei.This method implies that the correlation between the"phenotype"and"genotype"of nuclei is demonstrated through HTFC by creating a challenging link not yet explored between the aberrant morphological features of AML nuclei and NPM1 mutations.We conduct an ensemble-level statistical characterization of NPM1-wild type and NPM1-mutated blasts to discern their complex morphological and biophysical variances.Our findings suggest that characterizing cup-like nuclei in NPM1-related AML cells by HTFC may enhance the diagnostic approach for these tumors.Furthermore,we integrate virtual reality to provide an immersive fruition of morphological changes in AML cells within a true 3D environment.
文摘Holo-tomographic flow cytometry for label-free phenotyping of suspended acute myeloid leukemia blasts is demonstrated.A concave segmentation algorithm is applied to 3D refractive index tomograms to quantify NPM1-mutation-associated cup-like nuclear morphologies,with virtual reality visualization offering engaging immersion.The method enables population-level detection of statistically significant shifts in 3D cell morphology,originally correlating phenotype with genotype.
基金supported by the National Key Basic Research and Devel-opment Program of China (2010CB833603)the National Natural Science Foundation of China (30828023)
文摘Targeted drugs could significantly reduce cytotoxic effect and increase therapeutic activity. Dihydroartemisinin (DHA) has been shown to be effective in killing cancer cells. However, it exhibits non-targeted property. Holo-transferrin (TF) is a suitable drug-carrier to target cancer cells, because cancer cells need iron uptake by the TF-mediated mechanism to maintain their uncontrolled growth. Furthermore, TF receptors (TF-R) are highly expressed on cancer cell surfaces. In this paper, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the different killing effect of 4-(12-Dihydroartemisininoxy) Benzoic Acid Hydrozide-transferrin (DBAH-TF) on human breast cancer cells (MCF-7) cells and human normal breast (HNB) cells, and atomic force microscopy (AFM) was used to visually observe the targeted effect of DBAH-TF on MCF-7 cells. MTT results show that DBAH-TF is 172 times more potent than DHA in killing MCF-7 cells, while the cytotoxic effect of DBAH-TF on HNB cells is merely 1/33 to DHA. Also, the killing effect of DBAH-TF on MCF-7 cells is 286 times that on HNB cells, showing targeted effect. Moreover, there are distinct differences in ultrastructures of cellular surfaces after DBAH-TF and DHA treatment. Through AFM imaging, many characteristic holes were observed on the cancer cell surface after being effected by DBAH-TF, which differ from the holes with irregular shapes affected by DHA. These results visually show that the DBAH-TF targeted drug has more potent killing effect on MCF-7 cells compared with DHA.
