Germin and Germin-like protein （GLP） have various proposed roles in plant developmental stages and stress- related processes. A novel GLP cDNA clone was isolated from a cDNA library of Tamarix hispida. ThGLP, coded ...Germin and Germin-like protein （GLP） have various proposed roles in plant developmental stages and stress- related processes. A novel GLP cDNA clone was isolated from a cDNA library of Tamarix hispida. ThGLP, coded 225aa, possesses conserved motif of plant germin and Germin-like protein. ThGLP belongs to true germin subfamily through phylogenetic analyses. Gene expression profiles in roots and leaves were evaluated using real-time quantitative RT-PCR. The results show that the gene was highly induced by drought, salt, low temperature, CdCl2 and abscisic acid treatments. Our results demonstrate that the ThGLP gene is expressed in leaves and roots, is involved in different abiotic stress re-sponses and controlled by an ABA-dependent signaling pathway.展开更多
Ficus hispida L. (Moraceae) is a remarkable species in the ecosystem of tropical rainforests in Xishuangbanna, China. The figs and fig_pollination wasps (Chalcidoidae: Agaonidae) are highly co_evolved mutualists tha...Ficus hispida L. (Moraceae) is a remarkable species in the ecosystem of tropical rainforests in Xishuangbanna, China. The figs and fig_pollination wasps (Chalcidoidae: Agaonidae) are highly co_evolved mutualists that depend completely on each other for propagating descendants. Pollination of all fig species is done by fig wasps; their unique symbiotic associates, the fig wasps, cannot develop in anywhere except in the fig syconia. The present paper reports on the biology and flowering phenology of F. hispida , as well as the propagation character and pollination behavior of the fig wasps (Ceratosolen solmsi marchali Mayr) based on our observations in the rainforests of Xishuangbanna, southern Yunnan of China. F. hispida is a dioecious tree that annually blossoms and bears fruits 6-8 times, with four to five fruit_bearing peaks. The male trees produce pollen and provide fig wasps with reproductive havens, while the female trees produce fig seeds after pollination by the female wasps. Pollen of F. hispida cannot escape from the dehiscent anthers until they are disturbed by fig wasps. The female wasps open the anthers and collect pollen with their antennal scrapes, mandibles and legs, and then carry pollen to the female receptive syconia where fertilization takes place. Meanwhile, some of the female wasps lay eggs in the male receptive syconia. It takes about 3-67 min to search for the receptive syconia for pollination, and 15-23 h to enter the female receptive syconia. The number of female wasps entering a syconium has close relation with the impregnation and seed_bearing rate of female flowers, as well as the oviposition and reproduction rate of the fig wasps themselves. F. hispida is endowed with a relatively high level of seed bearing (54.1%-82.5%, average 73.8 %). The wasp oviposition rate on the male flowers is between 72.3% and 93.8% with a mean of 84.4%.展开更多
Trehalose is a non-reducing disaccharide with high stability and strong water absorption properties that can improve the resistance of organisms to various abiotic stresses.Trehalose-6-phosphate synthase(TPS)plays imp...Trehalose is a non-reducing disaccharide with high stability and strong water absorption properties that can improve the resistance of organisms to various abiotic stresses.Trehalose-6-phosphate synthase(TPS)plays important roles in trehalose metabolism and signaling.In this study,the full-length cDNA of ThTPS was cloned from Tamarix hispida Willd.A phylogenetic tree including ThTPS and 11 AtTPS genes from Arabidopsis indicated that the ThTPS protein had a close evolutionary relationship with AtTPS7.However,the function of At TPS7 has not been determined.To analyze the abiotic stress tolerance function of ThTPS,the expression of ThTPS in T.hispida under salt and drought stress and JA,ABA and GA3 hormone stimulation was monitored by qRT-PCR.The results show that ThTPS expression was clearly induced by all five of these treatments at one or more times,and salt stress caused particularly strong induction of Th TPS in the roots of T.hispida.The ThTPS gene was transiently overexpressed in T.hispida.Both physiological indexes and staining results showed that ThTPS gene overexpression increased salt and osmotic stress tolerance in T.hispida.Overall,the Th TPS gene can respond to abiotic stresses such as salt and drought,and its overexpression can significantly improve salt and osmotic tolerance.These findings establish a foundation to better understand the responses of TPS genes to abiotic stress in plants.展开更多
[ Objective] This study aimed to clone and analyze the expression of THWRKY12 gene in Tamarix hispida. [Method] T. hispida seedlings were treated with 400 mmol/L NaC1 solutlon, 20% PEG and 100 μmol/L ABA, respectivel...[ Objective] This study aimed to clone and analyze the expression of THWRKY12 gene in Tamarix hispida. [Method] T. hispida seedlings were treated with 400 mmol/L NaC1 solutlon, 20% PEG and 100 μmol/L ABA, respectively. The expression of THIVRKY12 gene in different tissues was investigated by RT-PCR technology. [Result] Under treatments of NaC1 and PEG, the expression of THWRKY12 gene in different tissues of T. h/sp/da seedlings showed an overall upregulated trend, suggesting that THWRKYI2 is related with the saline-alkali resistance and drought resistance of T. hispida. Under ABA treatment, THWRKY12 gene had approximately the same expression pattern with the former two, indicating that THWRKY12 gene may involve in the regulation and control of the saline-al- kali resistance and drought resistance of T. hispida through the signal pathway regulated by ABA. [ Conclusion] This study laid foundation for investigating the functions of WRKY gene in the stress resistance of T. hispida.展开更多
Four new alkaloids,fcuhismines A-D(1-4),together with three known ones,were isolated from Ficus hispida.Their structures were elucidated by spectroscopic analysis and chemical method.The new compounds represent the fr...Four new alkaloids,fcuhismines A-D(1-4),together with three known ones,were isolated from Ficus hispida.Their structures were elucidated by spectroscopic analysis and chemical method.The new compounds represent the frst amine alkaloids with a rhamnosyl moiety(1-2)or with a N-oxide motif(2-4)from the genus Ficus.Compound 2 showed potent inhibitory efect in nuclear factor-κB(NF-κB)pathway luciferase assay with IC_(50) value of 0.52±0.11μM.展开更多
基金This study was supported by national natural science foundation (Grant No. 30972386)Central university basic scientific business specific foundation (Grant No. DL09BA22)Genetically modified organisms breeding major projects (Grant No.2009ZX08009-098B)
文摘Germin and Germin-like protein （GLP） have various proposed roles in plant developmental stages and stress- related processes. A novel GLP cDNA clone was isolated from a cDNA library of Tamarix hispida. ThGLP, coded 225aa, possesses conserved motif of plant germin and Germin-like protein. ThGLP belongs to true germin subfamily through phylogenetic analyses. Gene expression profiles in roots and leaves were evaluated using real-time quantitative RT-PCR. The results show that the gene was highly induced by drought, salt, low temperature, CdCl2 and abscisic acid treatments. Our results demonstrate that the ThGLP gene is expressed in leaves and roots, is involved in different abiotic stress re-sponses and controlled by an ABA-dependent signaling pathway.
文摘Ficus hispida L. (Moraceae) is a remarkable species in the ecosystem of tropical rainforests in Xishuangbanna, China. The figs and fig_pollination wasps (Chalcidoidae: Agaonidae) are highly co_evolved mutualists that depend completely on each other for propagating descendants. Pollination of all fig species is done by fig wasps; their unique symbiotic associates, the fig wasps, cannot develop in anywhere except in the fig syconia. The present paper reports on the biology and flowering phenology of F. hispida , as well as the propagation character and pollination behavior of the fig wasps (Ceratosolen solmsi marchali Mayr) based on our observations in the rainforests of Xishuangbanna, southern Yunnan of China. F. hispida is a dioecious tree that annually blossoms and bears fruits 6-8 times, with four to five fruit_bearing peaks. The male trees produce pollen and provide fig wasps with reproductive havens, while the female trees produce fig seeds after pollination by the female wasps. Pollen of F. hispida cannot escape from the dehiscent anthers until they are disturbed by fig wasps. The female wasps open the anthers and collect pollen with their antennal scrapes, mandibles and legs, and then carry pollen to the female receptive syconia where fertilization takes place. Meanwhile, some of the female wasps lay eggs in the male receptive syconia. It takes about 3-67 min to search for the receptive syconia for pollination, and 15-23 h to enter the female receptive syconia. The number of female wasps entering a syconium has close relation with the impregnation and seed_bearing rate of female flowers, as well as the oviposition and reproduction rate of the fig wasps themselves. F. hispida is endowed with a relatively high level of seed bearing (54.1%-82.5%, average 73.8 %). The wasp oviposition rate on the male flowers is between 72.3% and 93.8% with a mean of 84.4%.
基金supported by the Province in Heilongjiang Outstanding Youth Science Fund(JC2017004)the National Natural Science Foundation of China(No.31370676)Heilongjiang Touyan Innovation Team Program(Tree Genetics and Breeding Innovation Team)。
文摘Trehalose is a non-reducing disaccharide with high stability and strong water absorption properties that can improve the resistance of organisms to various abiotic stresses.Trehalose-6-phosphate synthase(TPS)plays important roles in trehalose metabolism and signaling.In this study,the full-length cDNA of ThTPS was cloned from Tamarix hispida Willd.A phylogenetic tree including ThTPS and 11 AtTPS genes from Arabidopsis indicated that the ThTPS protein had a close evolutionary relationship with AtTPS7.However,the function of At TPS7 has not been determined.To analyze the abiotic stress tolerance function of ThTPS,the expression of ThTPS in T.hispida under salt and drought stress and JA,ABA and GA3 hormone stimulation was monitored by qRT-PCR.The results show that ThTPS expression was clearly induced by all five of these treatments at one or more times,and salt stress caused particularly strong induction of Th TPS in the roots of T.hispida.The ThTPS gene was transiently overexpressed in T.hispida.Both physiological indexes and staining results showed that ThTPS gene overexpression increased salt and osmotic stress tolerance in T.hispida.Overall,the Th TPS gene can respond to abiotic stresses such as salt and drought,and its overexpression can significantly improve salt and osmotic tolerance.These findings establish a foundation to better understand the responses of TPS genes to abiotic stress in plants.
基金Supported by College Students Innovative Experimental Project of Northeast Forestry University(No.201210225004)
文摘[ Objective] This study aimed to clone and analyze the expression of THWRKY12 gene in Tamarix hispida. [Method] T. hispida seedlings were treated with 400 mmol/L NaC1 solutlon, 20% PEG and 100 μmol/L ABA, respectively. The expression of THIVRKY12 gene in different tissues was investigated by RT-PCR technology. [Result] Under treatments of NaC1 and PEG, the expression of THWRKY12 gene in different tissues of T. h/sp/da seedlings showed an overall upregulated trend, suggesting that THWRKYI2 is related with the saline-alkali resistance and drought resistance of T. hispida. Under ABA treatment, THWRKY12 gene had approximately the same expression pattern with the former two, indicating that THWRKY12 gene may involve in the regulation and control of the saline-al- kali resistance and drought resistance of T. hispida through the signal pathway regulated by ABA. [ Conclusion] This study laid foundation for investigating the functions of WRKY gene in the stress resistance of T. hispida.
基金supports from the National Natural Science Foundation of China(Grant Nos.81222045 and 21672040)the Drug Innovation Major Project of China(Grant No.2018ZX09735001-002-006)are gratefully acknowledged.
文摘Four new alkaloids,fcuhismines A-D(1-4),together with three known ones,were isolated from Ficus hispida.Their structures were elucidated by spectroscopic analysis and chemical method.The new compounds represent the frst amine alkaloids with a rhamnosyl moiety(1-2)or with a N-oxide motif(2-4)from the genus Ficus.Compound 2 showed potent inhibitory efect in nuclear factor-κB(NF-κB)pathway luciferase assay with IC_(50) value of 0.52±0.11μM.
