The NRAMP(natural resistance-associated macrophage protein)family plays a pivotal role in metal ion transport,regulating both essential micronutrient uptake and toxic heavy metal accumulation in plants.In rice(Oryza s...The NRAMP(natural resistance-associated macrophage protein)family plays a pivotal role in metal ion transport,regulating both essential micronutrient uptake and toxic heavy metal accumulation in plants.In rice(Oryza sativa),OsNRAMP transporters critically influence metal homeostasis,stress adaptation,and grain safety.Among them,OsNRAMP5 serves as a major entry point for cadmium(Cd)and manganese(Mn)uptake,making it a prime target for low-Cd rice breeding.However,knockout of OsNRAMP5 leads to severe Mn deficiency,highlighting the need for precise genetic modifications(e.g.,OsNRAMP5-Q337K),which reduce Cd accumulation while maintaining Mn nutrition.Additionally,OsNRAMP1 and OsNRAMP2 contribute to Cd translocation and plant immunity,whereas OsNRAMP3/4/6/7 participate in Mn,iron,and zinc distribution and stress responses.This review systematically summarizes the structural,functional,and regulatory mechanisms of OsNRAMPs,emphasizing their roles in metal transport,pathogen resistance,and abiotic stress adaptation.Furthermore,we discuss strategies for developing low-Cd rice varieties,including QTL-based breeding,CRISPR/Cas9-mediated gene editing,and multi-gene stacking approaches.Finally,we outline future research directions,such as structural engineering of metal-binding sites and field validation of engineered rice lines,to ensure sustainable rice production in heavy metal-contaminated soils.展开更多
Multi-label feature selection(MFS)is a crucial dimensionality reduction technique aimed at identifying informative features associated with multiple labels.However,traditional centralized methods face significant chal...Multi-label feature selection(MFS)is a crucial dimensionality reduction technique aimed at identifying informative features associated with multiple labels.However,traditional centralized methods face significant challenges in privacy-sensitive and distributed settings,often neglecting label dependencies and suffering from low computational efficiency.To address these issues,we introduce a novel framework,Fed-MFSDHBCPSO—federated MFS via dual-layer hybrid breeding cooperative particle swarm optimization algorithm with manifold and sparsity regularization(DHBCPSO-MSR).Leveraging the federated learning paradigm,Fed-MFSDHBCPSO allows clients to perform local feature selection(FS)using DHBCPSO-MSR.Locally selected feature subsets are encrypted with differential privacy(DP)and transmitted to a central server,where they are securely aggregated and refined through secure multi-party computation(SMPC)until global convergence is achieved.Within each client,DHBCPSO-MSR employs a dual-layer FS strategy.The inner layer constructs sample and label similarity graphs,generates Laplacian matrices to capture the manifold structure between samples and labels,and applies L2,1-norm regularization to sparsify the feature subset,yielding an optimized feature weight matrix.The outer layer uses a hybrid breeding cooperative particle swarm optimization algorithm to further refine the feature weight matrix and identify the optimal feature subset.The updated weight matrix is then fed back to the inner layer for further optimization.Comprehensive experiments on multiple real-world multi-label datasets demonstrate that Fed-MFSDHBCPSO consistently outperforms both centralized and federated baseline methods across several key evaluation metrics.展开更多
The doubled haploid(DH)technique accelerates homozygosity by inducing chromosome doubling in haploid embryos derived from hybrid plants.This approach offers significant advantages over conventional rice breeding metho...The doubled haploid(DH)technique accelerates homozygosity by inducing chromosome doubling in haploid embryos derived from hybrid plants.This approach offers significant advantages over conventional rice breeding methods by shortening the breeding cycle and enabling rapid development of pure homozygous lines.Anther culture(AC)has been established as an efficient and successful method for producing DH plants via androgenesis in rice.However,despite its success in japonica rice.展开更多
‘Xiangchundou 36’is a new high-yield spring soybean cultivar bred by the Crop Research Institute of Hunan Province.This cultivar was bred in 2014 by the pedigree method with‘Xiangchundou V7’as the male parent and...‘Xiangchundou 36’is a new high-yield spring soybean cultivar bred by the Crop Research Institute of Hunan Province.This cultivar was bred in 2014 by the pedigree method with‘Xiangchundou V7’as the male parent and‘Youchun 1204’as the female parent.The cultivar showed high yields and excellent comprehensive traits in all levels of tests carried out from 2019 to 2023.In 2024,it was approved by Hunan Provincial Crop Variety Certification Committee,with the certification number Xiangshendou 20241004.‘Xiangchundou 36’was suitable for planting as a spring soybean cultivar in Hunan Province.展开更多
[Objective] Strain Biok Av-023 used as the control was employed on screening of high-avermectin yield mutants by rational screening.[Method] With Biok Av-023 as the original strain,the positive mutation strain was fir...[Objective] Strain Biok Av-023 used as the control was employed on screening of high-avermectin yield mutants by rational screening.[Method] With Biok Av-023 as the original strain,the positive mutation strain was firstly screened by routine UV mutagenesis,and then the high-yield avermectin producing strain was selected by the breeding way inferred by L-Ile induction.[Result] UV mutation and L-Ile directional screening had showed that the best L-Ile screening concentration was 0.5%,and the high-yield mutation strain AV60s-32 after re-screening reached the highest titer of 4520 IU/ml,which increased by 23.4% compared with the original strain.[Conclusion] The production of avermectin can be effectively enhanced by the combined way of UV mutation and L-Ile rational breeding.展开更多
Shuangyou 092, a Brassica napus cultivar with high oil content, high quality and high yield, was bred by Institute of Industrial Crops of Henan Academy of Agricultural Sciences with Zhongshuang 9 as female parent, and...Shuangyou 092, a Brassica napus cultivar with high oil content, high quality and high yield, was bred by Institute of Industrial Crops of Henan Academy of Agricultural Sciences with Zhongshuang 9 as female parent, and the cytoplasmic male sterility maintainer line 32B as male parent. It was registered and released in 2014. The average yield was 2 858.5 kg/hm^2 in Henan province regional test of high quality, 12.43% higher than the check variety Fengyou 9 in 2010-2011 and 2011-2012. Its yield was 3 478.8 kg/ha in Henan Province productive test of high quality,9.52% higher than the check variety Fengyou 9 in 2012-2013. According to the test results in 2011-2012 and 2012-2013, the seeds quality of Shuangyou 092 was very good with near zero erucic acid （0.0%, 0.1%） in fat acid, 17.19 μmol/g and 19.78 μmol/g glucosinolates in its meal, the oil content of 46.60% and 45.76%. The cultivar was resistant （tolerant） to Sclerotinia, virus diseases, cold, and lodging. Its high yield and high quality can be obtained by planting in large scale, sowing at right time, rational close planting, balancing fertilizer and harvesting at right time.展开更多
The security of the seed industry is crucial for ensuring national food security.Currently,developed countries in Europe and America,along with international seed industry giants,have entered the Breeding 4.0 era.This...The security of the seed industry is crucial for ensuring national food security.Currently,developed countries in Europe and America,along with international seed industry giants,have entered the Breeding 4.0 era.This era integrates biotechnology,artificial intelligence(AI),and big data information technology.In contrast,China is still in a transition period between stages 2.0 and 3.0,which primarily relies on conventional selection and molecular breeding.In the context of increasingly complex international situations,accurately identifying core issues in China's seed industry innovation and seizing the frontier of international seed technology are strategically important.These efforts are essential for ensuring food security and revitalizing the seed industry.This paper systematically analyzes the characteristics of crop breeding data from artificial selection to intelligent design breeding.It explores the applications and development trends of AI and big data in modern crop breeding from several key perspectives.These include highthroughput phenotype acquisition and analysis,multiomics big data database and management system construction,AI-based multiomics integrated analysis,and the development of intelligent breeding software tools based on biological big data and AI technology.Based on an in-depth analysis of the current status and challenges of China's seed industry technology development,we propose strategic goals and key tasks for China's new generation of AI and big data-driven intelligent design breeding.These suggestions aim to accelerate the development of an intelligent-driven crop breeding engineering system that features large-scale gene mining,efficient gene manipulation,engineered variety design,and systematized biobreeding.This study provides a theoretical basis and practical guidance for the development of China's seed industry technology.展开更多
Wheat rusts continue to cause significant losses worldwide despite major efforts given to their genetic control. This is due to frequent evolution and selection of virulence in pathogen overcoming the deployed race-sp...Wheat rusts continue to cause significant losses worldwide despite major efforts given to their genetic control. This is due to frequent evolution and selection of virulence in pathogen overcoming the deployed race-specific resistance genes. Although the life of effective race-specific resistance genes can be prolonged by using gene combinations, an alternative approach being implemented at CIMMYT is to deploy varieties that posses adult plant resistance （APR） based on combinations of minor, slow rusting genes. When present alone, the APR genes do not confer adequate resistance especially under high disease pressure; however, combinations of 4 or 5 minor genes usually result in ＂near-immunity＂ or a high level of resistance. Although only a few APR genes are catalogued, various APR QTLs are now known and could lead to further characterization of additional genes. Four characterized genes have pleiotropic effects in conferring partial APR to all 3 rusts and powdery mildew, thus simplifying the task of breeding wheat varieties that are resistant to multiple diseases. Significant progress was made recently in developing high-yielding wheat germplasm that possesses high levels of APR to all three rusts by implementing a Mexico- Kenya shuttle breeding scheme. Parents with APR to Ug99 were hybridized with high-yielding parents that had adequate to high levels of APR to leaf rust and yellow rust. Segregating populations and advanced lines from these crosses were selected under high rust pressures in Mexico （leaf rust and yellow rust） and Kenya （Ug99 stem rust and yellow rust） to identify high- yielding progenies that possess high to adequate APR to all three rusts. International distribution of these high-yielding wheats is underway through CIMMYT intemational yield trials and screening nurseries. It is expected that several wheat varieties with APR to three rusts will be released and grown in various countries in the near-future that will allow determining the durability of resistance.展开更多
Advancements in molecular approaches have been utilized to breed crops with a wide range of economically valuable traits to develop superior cultivars.This review provides a concise overview of modern breakthroughs in...Advancements in molecular approaches have been utilized to breed crops with a wide range of economically valuable traits to develop superior cultivars.This review provides a concise overview of modern breakthroughs in molecular plant production.Genotyping and high-throughput phenotyping methods for predictive plant breeding are briefly discussed.In this study,we explore contemporary molecular breeding techniques for producing desirable crop varieties.These techniques include cisgenesis,clustered regularly interspaced short palindromic repeat(CRISPR/Cas9)gene editing,haploid induction,and de novo domestication.We examine the speed breeding approach-a strategy for cultivating plants under controlled conditions.We further highlight the significance of modern breeding technologies in efficiently utilizing agricultural resources for crop production in urban areas.The deciphering of crop genomes has led to the development of extensive DNA markers,quantitative trait loci(QTLs),and pangenomes associated with various desirable crop traits.This shift to the genotypic selection of crops considerably expedites the plant breeding process.Based on the plant population used,the connection between genotypic and phenotypic data provides several genetic elements,including genes,markers,and alleles that can be used in genomic breeding and gene editing.The integration of speed breeding with genomic-assisted breeding and cutting-edge genome editing tools has made it feasible to rapidly manipulate and generate multiple crop cycles and accelerate the plant breeding process.Breakthroughs in molecular techniques have led to substantial improvements in modern breeding methods.展开更多
To meet the demand of maize production in Guangxi and even the southwest region of China and realize the upgrading and undating of maize varieties which will continuously improve the yield and quality of maize,a new b...To meet the demand of maize production in Guangxi and even the southwest region of China and realize the upgrading and undating of maize varieties which will continuously improve the yield and quality of maize,a new big-ear maize variety Guidan 903 with high yield,stable yield and good quality was established by the Maize Research Institute of Guangxi Academy of Agricultural Sciences with two self-selected inbred line GRL17349 and GRL21191 as female and male parents seperately.The average yield of this variety were 7429.5 and 7000.5 kg/hm^(2)in Guangxi Maize Regional Trial and Production Test respectively,which increased by 6.3%and 5.6%than the control(Guidan162).It was approved by Guangxi Zhuang Autonomous Region Crop Variety Certification Committee in June 2020(Approval No.Gui sheng yu 2020083).Determined by the Cereal Quality Supervision and Testing Center of Ministry of Agriculture and Rural Affairs(Bejjing),the grain bulk density,ratio of crude protein,crude fat,crude starch and lysine of Guidan 903 were 782 g/L,9.41%,6.19%,71.45%,0.35%respectively.This variety has the characterisics of strong resistance to collapse and disease,good quality,high and stable yield and wide adaptability.Furthermore,its seed yield is high with easy production technology and low cost.展开更多
Shanghua 511 is a high-yield, high-oil and web blotch-resistant big peanut variety bred by the Shangqiu Academy of Agriculture and Forestry Sciences through sexual hybridization （Yuhua 18♀×Shangyan 9658♂）. In...Shanghua 511 is a high-yield, high-oil and web blotch-resistant big peanut variety bred by the Shangqiu Academy of Agriculture and Forestry Sciences through sexual hybridization （Yuhua 18♀×Shangyan 9658♂）. In the wheat-peanut interplanting regional experiment conducted in Henan Province during 2012-2013, the average pod and seed yield of Shanghua 511 were 5906.19 and 4149.3 kg/hm2, respectively. In the wheat-peanut interplanting production experiment conducted in Henan Province in 2013, the average pod and seed yield of Shanghua 511 were 6036.45 and 4309.50 kg/hm2, respectively. In the kernels of Shanghua 511, the contents of crude fat, oleic acid and linoleic acid were 56.62%, 44.9% and 33.4% respectively, and the oleic/linoleic acid ratio was 1.34. This variety had high resistance to web blotch and medium resistance to leaf spot, viral diseases, root rot and collar rot. The 100-pod weight, 100-kernel weight, shelling percentage and growth period of Shanghua 511 were 270.6, 110.9 g, 70.6% and 120 d, respectively. Shanghua 511 passed the approval of Henan Provincial Crop Variety Approval Committee in August, 2015, and is suitable for spring sowing and wheat interplanting in the surrounding area of Henan Province and the area to the north of Huaihe River.展开更多
Computer simulation permits answering theoretical and applied questions in animal and plant breeding.Blib is a novel multi-module simulation platform,which is able to handle more complicated genetic effects and models...Computer simulation permits answering theoretical and applied questions in animal and plant breeding.Blib is a novel multi-module simulation platform,which is able to handle more complicated genetic effects and models than most existing tools.In this study,we describe one major and unified application module of Blib,i.e.,ISB(abbreviated from in silico breeding),for simulating the three categories of breeding programs for developing clonal,pure-line and hybrid cultivars in plants.Genetic models on environments and breeding-targeted traits,one or several parental populations,and a number of breeding methods are key elements to run simulation experiments in ISB,which are arranged in three external input files by given formats.Applications of ISB are illustrated by three case studies,representing the three categories of plant breeding programs.Under the condition that 5000 F1 progenies were generated and tested from 50 heterozygous parents,Case study I showed that 50 crosses,each of 100 progenies,made the best balance between genetic achievement and field cost.In Case study II,one optimum breeding method was identified by which the pure lines with high yield and medium maturity could be developed.Case study III investigated the genetic consequence in hybrid breeding from five testers.One tester was identified for the simultaneous improvement in F1 hybrids and inbred lines.In summary,ISB identified a balanced crossing scheme,an optimum pure-line selection method,and an optimized tester in three case studies which are relevant to plant breeding.We believe the prediction by simulation would be highly required in front of the next generation of breeding to be driven by informatics and intelligence.展开更多
Molecular tools have drawn the attention ofmodern plant breeders for its great precision and superiority.As the global population is increasing gradually,food production should be enhanced to feed the growing populati...Molecular tools have drawn the attention ofmodern plant breeders for its great precision and superiority.As the global population is increasing gradually,food production should be enhanced to feed the growing population.Therefore,precise and fast breeding tools are becoming obvious.Moreover,climate change has become a critical issue in crop improvement.Advanced breeding methods are vital to combat the impact of climate change,including biotic and abiotic stresses.Major molecular techniques,such as‘CRISPR-Cas’mediated‘genome editing’,‘marker-assisted selection(MAS)’,‘whole genome sequencing’,‘RNAi’,transgenic approach,‘high-throughput phenotyping(HTP)’,mutation breeding,have been proven superior over traditional breeding in terms of precision,efficiency,and speed in developing stress-resistant improved varieties.This review explores the potential and superiority ofmolecular breeding methods and highlights the gaps(time,cost,efficiency,etc.)in traditional breeding methods,where modern breeding programs,asmentioned,are effective.Furthermore,this reviewwill focus on the necessity of keymodern plant breeding techniques as a foundation for sustainable farming practices to address emerging environmental challenges,ensure food security,and improve the yield and quality of crops.展开更多
This study uses prefecture-level city statistical data from China from 2000-2022 to measure the supply and demand of livestock manure nitrogen nutrients and calculates farmland livestock carrying capacity using the ni...This study uses prefecture-level city statistical data from China from 2000-2022 to measure the supply and demand of livestock manure nitrogen nutrients and calculates farmland livestock carrying capacity using the nitrogen nutrient balance method.We investigate nitrogen supply and demand and livestock carrying capacity in northeast China by comparing emissions from 2000-2022 over the past several decades.The poultry and livestock industry in northeast China has changed significantly over the past two decades:pigs are now the most bred animal and poultry production has increased dramatically.Regional livestock nitrogen emissions are influenced primarily by the size of the local livestock industry chain.Due to regional differences,each region has unique breeding structures.We also predict the anticipated situation in 2050 using the business-as usual scenario.High-risk livestock carrying capacity areas will be concentrated in the northeast and southeast regions,with significantly increased risk indices,compared with those of 2022,particularly in Shenyang,Fushun,and Tieling.Therefore,promoting farming and breeding,improving livestock manure utilization,and returning manure to nearby farmlands are crucial for meeting agricultural green development goals.展开更多
Crop disease is best combated with host resistance,this approach rests on an understanding of pathogen-host interaction mechanisms and the availability of resistance genes and breeding materials.This special issue col...Crop disease is best combated with host resistance,this approach rests on an understanding of pathogen-host interaction mechanisms and the availability of resistance genes and breeding materials.This special issue collects 12 articles reporting recent progress in crop disease-resistance research.展开更多
Soybean(Glycine max)is a vital foundation of global food security,providing a primary source of highquality protein and oil for human consumption and animal feed.The rising global population has significantly increase...Soybean(Glycine max)is a vital foundation of global food security,providing a primary source of highquality protein and oil for human consumption and animal feed.The rising global population has significantly increased the demand for soybeans,emphasizing the urgency of developing high-yield,stresstolerant,and nutritionally superior cultivars.The extensive collection of soybean germplasm resources—including wild relatives,landraces,and cultivars—represents a valuable reservoir of genetic diversity critical for breeding advancements.Recent breakthroughs in genomic technologies,particularly highthroughput sequencing and multi-omics approaches,have revolutionized the identification of key genes associated with essential agronomic traits within these resources.These innovations enable precise and strategic utilization of genetic diversity,empowering breeders to integrate traits that improve yield potential,resilience to biotic and abiotic stresses,and nutritional quality.This review highlights the critical role of genetic resources and omics-driven innovations in soybean breeding.It also offers insights into strategies for accelerating the development of elite soybean cultivars to meet the growing demands of global soybean production.展开更多
A predictive model of meiotic crossover engineering would increase precision in crop breeding.We review the biological principles underlying crossover formation and chromosomal distribution,hierarchical control mechan...A predictive model of meiotic crossover engineering would increase precision in crop breeding.We review the biological principles underlying crossover formation and chromosomal distribution,hierarchical control mechanisms enforcing crossover assurance,and an emerging phase-separation model determining crossover interference patterning.展开更多
Low energy ion beam has been widely applied in microbe breeding, plant breeding, gene transfer and cell modification. In this study, the Escherichia coli （E.coli） strain producing tryptophanase was irradiated by a l...Low energy ion beam has been widely applied in microbe breeding, plant breeding, gene transfer and cell modification. In this study, the Escherichia coli （E.coli） strain producing tryptophanase was irradiated by a low energy nitrogen ion beam with an energy of 10 keV at a fluence of 13 × 10^14 N^＋/cm^2 when glycerin at a 15% concentration was used as a protector. The effect on the biomass of E. coli after N^＋ implantation was analyzed in detail by statistic methods. The screening methods used in this study were proven to be effective. After continuous mutagenicity, a high-yield tryptophanase strain was selected and both its biomass and enzymatic activity were higher than those of the parent strain. The results of scale-up production showed that the biomass could reach wet weight 8.2 g/L and 110 g L-tryptophan could be formed in the volume of the 1 l enzymatic reaction system.展开更多
Extensive transcriptomic reprogramming is triggered by biotic and abiotic stresses in plants,with coordinated regulation mediated through multiple transcription factor families,such as WRKY,MYB,NAC,and BBX proteins.Am...Extensive transcriptomic reprogramming is triggered by biotic and abiotic stresses in plants,with coordinated regulation mediated through multiple transcription factor families,such as WRKY,MYB,NAC,and BBX proteins.Among these,B-box(BBX)proteins represent a distinct class of zinc finger transcription factors characterized by the presence of conserved B-box domains.They serve as central regulators in plant photomorphogenesis and developmental processes.Accumulating genetic and biochemical evidence demonstrates that BBX family members orchestrate plant responses to biotic and abiotic stresses through multifaceted molecular mechanisms,including the regulation of reactive oxygen species(ROS)homeostasis,enhancement of anthocyanin biosynthesis,and modulation of hormonal signaling pathways.This review systematically summarizes recent advances in the identification of BBX family genes in different plant species.Furthermore,their emerging roles in mediating plant stress responses are elucidated,with molecularmechanisms being comprehensively analyzed at both transcriptional and post-translational levels.However,to fully harness the potential of BBX genes in crop improvement,a deeper understanding of their functional mechanisms including BBX-mediated hormonal crosstalk networks,growth-defense trade-offs,and more extensive field performance data remains essential.These insights provide a theoretical foundation for developing climate-resilient crop varieties through targeted genetic improvement strategies.展开更多
The identification of haploid induction genes has promoted the advancement of several breeding technologies.Haploid induction genes in wheat,coupled with visual marker,have led to the establishment of a novel in vivo ...The identification of haploid induction genes has promoted the advancement of several breeding technologies.Haploid induction genes in wheat,coupled with visual marker,have led to the establishment of a novel in vivo doubled-haploid(DH)technology.When combined with dominant male sterile genes,this innovative DH method presents a promising avenue for high-throughput production of DH lines.Furthermore,the application of haploid induction genes has facilitated the establishment of other innovative breeding technologies,such as HI-Edit and cyto-swapping in creating cytoplasmic male sterility lines,as well as synthetic apomixis.This review summarizes the progress of DH technology in wheat and presents examples of application of haploid induction genes in accelerating breeding practices,aiming to promote the development of these innovative technologies in wheat and enhancing wheat breeding efficiency.展开更多
基金supported by the National Key R&D Program,China(Grant No.2022YFD1201505)the Key Laboratory of Sichuan Province Open Project,China(Grant No.2023LYKF02)+1 种基金the Central Public-Interest Scientific Institution Basal Research Fund,China(Grant No.CPSIBRF-CNRRI-202306)the Sichuan Provincial Financial Independent Innovation Project,China(Grant No.2022ZZCX001).
文摘The NRAMP(natural resistance-associated macrophage protein)family plays a pivotal role in metal ion transport,regulating both essential micronutrient uptake and toxic heavy metal accumulation in plants.In rice(Oryza sativa),OsNRAMP transporters critically influence metal homeostasis,stress adaptation,and grain safety.Among them,OsNRAMP5 serves as a major entry point for cadmium(Cd)and manganese(Mn)uptake,making it a prime target for low-Cd rice breeding.However,knockout of OsNRAMP5 leads to severe Mn deficiency,highlighting the need for precise genetic modifications(e.g.,OsNRAMP5-Q337K),which reduce Cd accumulation while maintaining Mn nutrition.Additionally,OsNRAMP1 and OsNRAMP2 contribute to Cd translocation and plant immunity,whereas OsNRAMP3/4/6/7 participate in Mn,iron,and zinc distribution and stress responses.This review systematically summarizes the structural,functional,and regulatory mechanisms of OsNRAMPs,emphasizing their roles in metal transport,pathogen resistance,and abiotic stress adaptation.Furthermore,we discuss strategies for developing low-Cd rice varieties,including QTL-based breeding,CRISPR/Cas9-mediated gene editing,and multi-gene stacking approaches.Finally,we outline future research directions,such as structural engineering of metal-binding sites and field validation of engineered rice lines,to ensure sustainable rice production in heavy metal-contaminated soils.
文摘Multi-label feature selection(MFS)is a crucial dimensionality reduction technique aimed at identifying informative features associated with multiple labels.However,traditional centralized methods face significant challenges in privacy-sensitive and distributed settings,often neglecting label dependencies and suffering from low computational efficiency.To address these issues,we introduce a novel framework,Fed-MFSDHBCPSO—federated MFS via dual-layer hybrid breeding cooperative particle swarm optimization algorithm with manifold and sparsity regularization(DHBCPSO-MSR).Leveraging the federated learning paradigm,Fed-MFSDHBCPSO allows clients to perform local feature selection(FS)using DHBCPSO-MSR.Locally selected feature subsets are encrypted with differential privacy(DP)and transmitted to a central server,where they are securely aggregated and refined through secure multi-party computation(SMPC)until global convergence is achieved.Within each client,DHBCPSO-MSR employs a dual-layer FS strategy.The inner layer constructs sample and label similarity graphs,generates Laplacian matrices to capture the manifold structure between samples and labels,and applies L2,1-norm regularization to sparsify the feature subset,yielding an optimized feature weight matrix.The outer layer uses a hybrid breeding cooperative particle swarm optimization algorithm to further refine the feature weight matrix and identify the optimal feature subset.The updated weight matrix is then fed back to the inner layer for further optimization.Comprehensive experiments on multiple real-world multi-label datasets demonstrate that Fed-MFSDHBCPSO consistently outperforms both centralized and federated baseline methods across several key evaluation metrics.
基金supported by the Science and Technology Innovation Program of Fujian Agriculture and Forestry University,China(Grant No.KFB22045)the General Program of Natural Science Foundation Fujian,China(Grant No.2023J01460).
文摘The doubled haploid(DH)technique accelerates homozygosity by inducing chromosome doubling in haploid embryos derived from hybrid plants.This approach offers significant advantages over conventional rice breeding methods by shortening the breeding cycle and enabling rapid development of pure homozygous lines.Anther culture(AC)has been established as an efficient and successful method for producing DH plants via androgenesis in rice.However,despite its success in japonica rice.
文摘‘Xiangchundou 36’is a new high-yield spring soybean cultivar bred by the Crop Research Institute of Hunan Province.This cultivar was bred in 2014 by the pedigree method with‘Xiangchundou V7’as the male parent and‘Youchun 1204’as the female parent.The cultivar showed high yields and excellent comprehensive traits in all levels of tests carried out from 2019 to 2023.In 2024,it was approved by Hunan Provincial Crop Variety Certification Committee,with the certification number Xiangshendou 20241004.‘Xiangchundou 36’was suitable for planting as a spring soybean cultivar in Hunan Province.
基金Supported by 863 Project (2009AA032904 )Scientific ResearchStarting-up Project for Young Teachers in Changshu Institute oTechnology" Hundreds of Entrepreneurs into Campus" Project inChangshu Institute of Technology~~
文摘[Objective] Strain Biok Av-023 used as the control was employed on screening of high-avermectin yield mutants by rational screening.[Method] With Biok Av-023 as the original strain,the positive mutation strain was firstly screened by routine UV mutagenesis,and then the high-yield avermectin producing strain was selected by the breeding way inferred by L-Ile induction.[Result] UV mutation and L-Ile directional screening had showed that the best L-Ile screening concentration was 0.5%,and the high-yield mutation strain AV60s-32 after re-screening reached the highest titer of 4520 IU/ml,which increased by 23.4% compared with the original strain.[Conclusion] The production of avermectin can be effectively enhanced by the combined way of UV mutation and L-Ile rational breeding.
文摘Shuangyou 092, a Brassica napus cultivar with high oil content, high quality and high yield, was bred by Institute of Industrial Crops of Henan Academy of Agricultural Sciences with Zhongshuang 9 as female parent, and the cytoplasmic male sterility maintainer line 32B as male parent. It was registered and released in 2014. The average yield was 2 858.5 kg/hm^2 in Henan province regional test of high quality, 12.43% higher than the check variety Fengyou 9 in 2010-2011 and 2011-2012. Its yield was 3 478.8 kg/ha in Henan Province productive test of high quality,9.52% higher than the check variety Fengyou 9 in 2012-2013. According to the test results in 2011-2012 and 2012-2013, the seeds quality of Shuangyou 092 was very good with near zero erucic acid （0.0%, 0.1%） in fat acid, 17.19 μmol/g and 19.78 μmol/g glucosinolates in its meal, the oil content of 46.60% and 45.76%. The cultivar was resistant （tolerant） to Sclerotinia, virus diseases, cold, and lodging. Its high yield and high quality can be obtained by planting in large scale, sowing at right time, rational close planting, balancing fertilizer and harvesting at right time.
基金partially supported by the Construction of Collaborative Innovation Center of Beijing Academy of Agricultural and Forestry Sciences(KJCX20240406)the Beijing Natural Science Foundation(JQ24037)+1 种基金the National Natural Science Foundation of China(32330075)the Earmarked Fund for China Agriculture Research System(CARS-02 and CARS-54)。
文摘The security of the seed industry is crucial for ensuring national food security.Currently,developed countries in Europe and America,along with international seed industry giants,have entered the Breeding 4.0 era.This era integrates biotechnology,artificial intelligence(AI),and big data information technology.In contrast,China is still in a transition period between stages 2.0 and 3.0,which primarily relies on conventional selection and molecular breeding.In the context of increasingly complex international situations,accurately identifying core issues in China's seed industry innovation and seizing the frontier of international seed technology are strategically important.These efforts are essential for ensuring food security and revitalizing the seed industry.This paper systematically analyzes the characteristics of crop breeding data from artificial selection to intelligent design breeding.It explores the applications and development trends of AI and big data in modern crop breeding from several key perspectives.These include highthroughput phenotype acquisition and analysis,multiomics big data database and management system construction,AI-based multiomics integrated analysis,and the development of intelligent breeding software tools based on biological big data and AI technology.Based on an in-depth analysis of the current status and challenges of China's seed industry technology development,we propose strategic goals and key tasks for China's new generation of AI and big data-driven intelligent design breeding.These suggestions aim to accelerate the development of an intelligent-driven crop breeding engineering system that features large-scale gene mining,efficient gene manipulation,engineered variety design,and systematized biobreeding.This study provides a theoretical basis and practical guidance for the development of China's seed industry technology.
基金financial resources from the Durable Rust Resistant Wheat Project led by Cornell University,USAsupported by the Bill & Melinda Gates Foundation+6 种基金ICAR-IndiaUSDA-ARS and USAID,USAGRDC AustraliaAgrovegetal-Spainthe Northwestern Mexican Farmer Association (Patronato) and CONFUPRO,MexicoSDC,SwitzerlandCIMMYT and INIFAP
文摘Wheat rusts continue to cause significant losses worldwide despite major efforts given to their genetic control. This is due to frequent evolution and selection of virulence in pathogen overcoming the deployed race-specific resistance genes. Although the life of effective race-specific resistance genes can be prolonged by using gene combinations, an alternative approach being implemented at CIMMYT is to deploy varieties that posses adult plant resistance （APR） based on combinations of minor, slow rusting genes. When present alone, the APR genes do not confer adequate resistance especially under high disease pressure; however, combinations of 4 or 5 minor genes usually result in ＂near-immunity＂ or a high level of resistance. Although only a few APR genes are catalogued, various APR QTLs are now known and could lead to further characterization of additional genes. Four characterized genes have pleiotropic effects in conferring partial APR to all 3 rusts and powdery mildew, thus simplifying the task of breeding wheat varieties that are resistant to multiple diseases. Significant progress was made recently in developing high-yielding wheat germplasm that possesses high levels of APR to all three rusts by implementing a Mexico- Kenya shuttle breeding scheme. Parents with APR to Ug99 were hybridized with high-yielding parents that had adequate to high levels of APR to leaf rust and yellow rust. Segregating populations and advanced lines from these crosses were selected under high rust pressures in Mexico （leaf rust and yellow rust） and Kenya （Ug99 stem rust and yellow rust） to identify high- yielding progenies that possess high to adequate APR to all three rusts. International distribution of these high-yielding wheats is underway through CIMMYT intemational yield trials and screening nurseries. It is expected that several wheat varieties with APR to three rusts will be released and grown in various countries in the near-future that will allow determining the durability of resistance.
基金funded by the United Arab Emirates UniversityResearch Officegrant number 12F041 to KM。
文摘Advancements in molecular approaches have been utilized to breed crops with a wide range of economically valuable traits to develop superior cultivars.This review provides a concise overview of modern breakthroughs in molecular plant production.Genotyping and high-throughput phenotyping methods for predictive plant breeding are briefly discussed.In this study,we explore contemporary molecular breeding techniques for producing desirable crop varieties.These techniques include cisgenesis,clustered regularly interspaced short palindromic repeat(CRISPR/Cas9)gene editing,haploid induction,and de novo domestication.We examine the speed breeding approach-a strategy for cultivating plants under controlled conditions.We further highlight the significance of modern breeding technologies in efficiently utilizing agricultural resources for crop production in urban areas.The deciphering of crop genomes has led to the development of extensive DNA markers,quantitative trait loci(QTLs),and pangenomes associated with various desirable crop traits.This shift to the genotypic selection of crops considerably expedites the plant breeding process.Based on the plant population used,the connection between genotypic and phenotypic data provides several genetic elements,including genes,markers,and alleles that can be used in genomic breeding and gene editing.The integration of speed breeding with genomic-assisted breeding and cutting-edge genome editing tools has made it feasible to rapidly manipulate and generate multiple crop cycles and accelerate the plant breeding process.Breakthroughs in molecular techniques have led to substantial improvements in modern breeding methods.
基金Special Fund for Basal Scientific Research of Guangxi Academy of Agricultural Sciences(GNK 2021YT017,GNK 2017YM08)Science and Technology Development Fund of Maize Research Institute,Guangxi Academy of Agricultural Sciences(GYK 2017010)Natural Science Foundation Project of Guangxi(2018JJB130196).
文摘To meet the demand of maize production in Guangxi and even the southwest region of China and realize the upgrading and undating of maize varieties which will continuously improve the yield and quality of maize,a new big-ear maize variety Guidan 903 with high yield,stable yield and good quality was established by the Maize Research Institute of Guangxi Academy of Agricultural Sciences with two self-selected inbred line GRL17349 and GRL21191 as female and male parents seperately.The average yield of this variety were 7429.5 and 7000.5 kg/hm^(2)in Guangxi Maize Regional Trial and Production Test respectively,which increased by 6.3%and 5.6%than the control(Guidan162).It was approved by Guangxi Zhuang Autonomous Region Crop Variety Certification Committee in June 2020(Approval No.Gui sheng yu 2020083).Determined by the Cereal Quality Supervision and Testing Center of Ministry of Agriculture and Rural Affairs(Bejjing),the grain bulk density,ratio of crude protein,crude fat,crude starch and lysine of Guidan 903 were 782 g/L,9.41%,6.19%,71.45%,0.35%respectively.This variety has the characterisics of strong resistance to collapse and disease,good quality,high and stable yield and wide adaptability.Furthermore,its seed yield is high with easy production technology and low cost.
基金Supported by Genetic Breeding Post Special Fund of Peanut Industrial Technology System in Henan Province(S2012-05-G01)Major Science and Technology Program of Henan Province(141100110600,161100111000)~~
文摘Shanghua 511 is a high-yield, high-oil and web blotch-resistant big peanut variety bred by the Shangqiu Academy of Agriculture and Forestry Sciences through sexual hybridization （Yuhua 18♀×Shangyan 9658♂）. In the wheat-peanut interplanting regional experiment conducted in Henan Province during 2012-2013, the average pod and seed yield of Shanghua 511 were 5906.19 and 4149.3 kg/hm2, respectively. In the wheat-peanut interplanting production experiment conducted in Henan Province in 2013, the average pod and seed yield of Shanghua 511 were 6036.45 and 4309.50 kg/hm2, respectively. In the kernels of Shanghua 511, the contents of crude fat, oleic acid and linoleic acid were 56.62%, 44.9% and 33.4% respectively, and the oleic/linoleic acid ratio was 1.34. This variety had high resistance to web blotch and medium resistance to leaf spot, viral diseases, root rot and collar rot. The 100-pod weight, 100-kernel weight, shelling percentage and growth period of Shanghua 511 were 270.6, 110.9 g, 70.6% and 120 d, respectively. Shanghua 511 passed the approval of Henan Provincial Crop Variety Approval Committee in August, 2015, and is suitable for spring sowing and wheat interplanting in the surrounding area of Henan Province and the area to the north of Huaihe River.
基金supported by Biological Breeding-National Science and Technology Major Project(2023ZD0407501)National Natural Science Foundation of China(31861143003)Innovation Program of Chinese Academy of Agricultural Sciences.
文摘Computer simulation permits answering theoretical and applied questions in animal and plant breeding.Blib is a novel multi-module simulation platform,which is able to handle more complicated genetic effects and models than most existing tools.In this study,we describe one major and unified application module of Blib,i.e.,ISB(abbreviated from in silico breeding),for simulating the three categories of breeding programs for developing clonal,pure-line and hybrid cultivars in plants.Genetic models on environments and breeding-targeted traits,one or several parental populations,and a number of breeding methods are key elements to run simulation experiments in ISB,which are arranged in three external input files by given formats.Applications of ISB are illustrated by three case studies,representing the three categories of plant breeding programs.Under the condition that 5000 F1 progenies were generated and tested from 50 heterozygous parents,Case study I showed that 50 crosses,each of 100 progenies,made the best balance between genetic achievement and field cost.In Case study II,one optimum breeding method was identified by which the pure lines with high yield and medium maturity could be developed.Case study III investigated the genetic consequence in hybrid breeding from five testers.One tester was identified for the simultaneous improvement in F1 hybrids and inbred lines.In summary,ISB identified a balanced crossing scheme,an optimum pure-line selection method,and an optimized tester in three case studies which are relevant to plant breeding.We believe the prediction by simulation would be highly required in front of the next generation of breeding to be driven by informatics and intelligence.
文摘Molecular tools have drawn the attention ofmodern plant breeders for its great precision and superiority.As the global population is increasing gradually,food production should be enhanced to feed the growing population.Therefore,precise and fast breeding tools are becoming obvious.Moreover,climate change has become a critical issue in crop improvement.Advanced breeding methods are vital to combat the impact of climate change,including biotic and abiotic stresses.Major molecular techniques,such as‘CRISPR-Cas’mediated‘genome editing’,‘marker-assisted selection(MAS)’,‘whole genome sequencing’,‘RNAi’,transgenic approach,‘high-throughput phenotyping(HTP)’,mutation breeding,have been proven superior over traditional breeding in terms of precision,efficiency,and speed in developing stress-resistant improved varieties.This review explores the potential and superiority ofmolecular breeding methods and highlights the gaps(time,cost,efficiency,etc.)in traditional breeding methods,where modern breeding programs,asmentioned,are effective.Furthermore,this reviewwill focus on the necessity of keymodern plant breeding techniques as a foundation for sustainable farming practices to address emerging environmental challenges,ensure food security,and improve the yield and quality of crops.
基金supported by the National Natural Science Foundation of Youth Project“Research on the Environmental-economic Synergistic Mechanism and Promotion Strategy of Farming and Breeding Circular Pattern in the Black Soil Region of Northeast China”[Grant No.72303087].
文摘This study uses prefecture-level city statistical data from China from 2000-2022 to measure the supply and demand of livestock manure nitrogen nutrients and calculates farmland livestock carrying capacity using the nitrogen nutrient balance method.We investigate nitrogen supply and demand and livestock carrying capacity in northeast China by comparing emissions from 2000-2022 over the past several decades.The poultry and livestock industry in northeast China has changed significantly over the past two decades:pigs are now the most bred animal and poultry production has increased dramatically.Regional livestock nitrogen emissions are influenced primarily by the size of the local livestock industry chain.Due to regional differences,each region has unique breeding structures.We also predict the anticipated situation in 2050 using the business-as usual scenario.High-risk livestock carrying capacity areas will be concentrated in the northeast and southeast regions,with significantly increased risk indices,compared with those of 2022,particularly in Shenyang,Fushun,and Tieling.Therefore,promoting farming and breeding,improving livestock manure utilization,and returning manure to nearby farmlands are crucial for meeting agricultural green development goals.
文摘Crop disease is best combated with host resistance,this approach rests on an understanding of pathogen-host interaction mechanisms and the availability of resistance genes and breeding materials.This special issue collects 12 articles reporting recent progress in crop disease-resistance research.
基金supported by the National Key Research and Development Program of China(2022YFF1003301,2023YFF1000101,2022YFE0130200)the Taishan Scholars Program。
文摘Soybean(Glycine max)is a vital foundation of global food security,providing a primary source of highquality protein and oil for human consumption and animal feed.The rising global population has significantly increased the demand for soybeans,emphasizing the urgency of developing high-yield,stresstolerant,and nutritionally superior cultivars.The extensive collection of soybean germplasm resources—including wild relatives,landraces,and cultivars—represents a valuable reservoir of genetic diversity critical for breeding advancements.Recent breakthroughs in genomic technologies,particularly highthroughput sequencing and multi-omics approaches,have revolutionized the identification of key genes associated with essential agronomic traits within these resources.These innovations enable precise and strategic utilization of genetic diversity,empowering breeders to integrate traits that improve yield potential,resilience to biotic and abiotic stresses,and nutritional quality.This review highlights the critical role of genetic resources and omics-driven innovations in soybean breeding.It also offers insights into strategies for accelerating the development of elite soybean cultivars to meet the growing demands of global soybean production.
基金funded by the National Natural Science Foundation of China(U2102219 and 32370901)the Project of Zhongshan Biological Breeding Laboratory(ZSBBL-KY2023-06-3).
文摘A predictive model of meiotic crossover engineering would increase precision in crop breeding.We review the biological principles underlying crossover formation and chromosomal distribution,hierarchical control mechanisms enforcing crossover assurance,and an emerging phase-separation model determining crossover interference patterning.
文摘Low energy ion beam has been widely applied in microbe breeding, plant breeding, gene transfer and cell modification. In this study, the Escherichia coli （E.coli） strain producing tryptophanase was irradiated by a low energy nitrogen ion beam with an energy of 10 keV at a fluence of 13 × 10^14 N^＋/cm^2 when glycerin at a 15% concentration was used as a protector. The effect on the biomass of E. coli after N^＋ implantation was analyzed in detail by statistic methods. The screening methods used in this study were proven to be effective. After continuous mutagenicity, a high-yield tryptophanase strain was selected and both its biomass and enzymatic activity were higher than those of the parent strain. The results of scale-up production showed that the biomass could reach wet weight 8.2 g/L and 110 g L-tryptophan could be formed in the volume of the 1 l enzymatic reaction system.
基金National Natural Science Foundation of China(grant No.32301870 and 32572302 to Chen Lin)Natural Science Foundation of Jiangsu Province(grant No.BK20230568 to Chen Lin)+3 种基金the Jiangsu Provincial Agricultural Science and Technology Independent Innovation Fund(grant No.CX(24)3124 to Chen Lin)Outstanding Ph.D.Program in Yangzhou(grant No.YZLYJFJH2022YXBS147 to Chen Lin)the General Project of Basic Scientific Research to colleges and universities in Jiangsu Province(grant No.22KJB210019 to Chen Lin)the Priority Academic Program Development of Jiangsu Higher Education Institutions is greatly acknowledged.
文摘Extensive transcriptomic reprogramming is triggered by biotic and abiotic stresses in plants,with coordinated regulation mediated through multiple transcription factor families,such as WRKY,MYB,NAC,and BBX proteins.Among these,B-box(BBX)proteins represent a distinct class of zinc finger transcription factors characterized by the presence of conserved B-box domains.They serve as central regulators in plant photomorphogenesis and developmental processes.Accumulating genetic and biochemical evidence demonstrates that BBX family members orchestrate plant responses to biotic and abiotic stresses through multifaceted molecular mechanisms,including the regulation of reactive oxygen species(ROS)homeostasis,enhancement of anthocyanin biosynthesis,and modulation of hormonal signaling pathways.This review systematically summarizes recent advances in the identification of BBX family genes in different plant species.Furthermore,their emerging roles in mediating plant stress responses are elucidated,with molecularmechanisms being comprehensively analyzed at both transcriptional and post-translational levels.However,to fully harness the potential of BBX genes in crop improvement,a deeper understanding of their functional mechanisms including BBX-mediated hormonal crosstalk networks,growth-defense trade-offs,and more extensive field performance data remains essential.These insights provide a theoretical foundation for developing climate-resilient crop varieties through targeted genetic improvement strategies.
基金financially supported by the Jiangsu Provincial Key R&D Program(Modern Agriculture)(BE2023313)the National Natural Science Foundation of China(31901535)the China Agricultural Research System(CARS-03).
文摘The identification of haploid induction genes has promoted the advancement of several breeding technologies.Haploid induction genes in wheat,coupled with visual marker,have led to the establishment of a novel in vivo doubled-haploid(DH)technology.When combined with dominant male sterile genes,this innovative DH method presents a promising avenue for high-throughput production of DH lines.Furthermore,the application of haploid induction genes has facilitated the establishment of other innovative breeding technologies,such as HI-Edit and cyto-swapping in creating cytoplasmic male sterility lines,as well as synthetic apomixis.This review summarizes the progress of DH technology in wheat and presents examples of application of haploid induction genes in accelerating breeding practices,aiming to promote the development of these innovative technologies in wheat and enhancing wheat breeding efficiency.
