Coconut(Cocos nucifera L.),a major oil and fruit crop of the Arecaceae family,is extensively cultivated across the Asia—Pacific region.Despite its agricultural importance,genome assembly in coconut remains challengin...Coconut(Cocos nucifera L.),a major oil and fruit crop of the Arecaceae family,is extensively cultivated across the Asia—Pacific region.Despite its agricultural importance,genome assembly in coconut remains challenging due to its large genome size and high proportion of repetitive sequences.Allele-specific expression(ASE)plays a key role in regulating plant development and evolution,yet research on ASE in coconut is limited(Shao et al.,2019;Li et al.,2021;Zhang et al.,2021;Hu et al.,2022).Among phenotypic traits,fruit color is especially important as an indicator of maturity,guiding harvest timing and post-harvest processes(Kapoor et al.,2022).While prior studies have explored various coconut traits such as salt tolerance,fiber content,and plant height(Wang et al.,2021;Yang et al.,2021),investigations into ASE and fruit color remain scarce.展开更多
Artificial intelligence,such as deep learning technology,has advanced the study of facial expression recognition since facial expression carries rich emotional information and is significant for many naturalistic situ...Artificial intelligence,such as deep learning technology,has advanced the study of facial expression recognition since facial expression carries rich emotional information and is significant for many naturalistic situations.To pursue a high facial expression recognition accuracy,the network model of deep learning is generally designed to be very deep while the model’s real-time performance is typically constrained and limited.With MobileNetV3,a lightweight model with a good accuracy,a further study is conducted by adding a basic ResNet module to each of its existing modules and an SSH(Single Stage Headless Face Detector)context module to expand the model’s perceptual field.In this article,the enhanced model named Res-MobileNetV3,could alleviate the subpar of real-time performance and compress the size of large network models,which can process information at a rate of up to 33 frames per second.Although the improved model has been verified to be slightly inferior to the current state-of-the-art method in aspect of accuracy rate on the publically available face expression datasets,it can bring a good balance on accuracy,real-time performance,model size and model complexity in practical applications.展开更多
Rice is a poor source of folate,an essential micronutrient for the body.Biofortification offers an effective way to enhance the folate content of rice and alleviate folate deficiencies in humans.In this study,we confi...Rice is a poor source of folate,an essential micronutrient for the body.Biofortification offers an effective way to enhance the folate content of rice and alleviate folate deficiencies in humans.In this study,we confirmed that OsADCS and OsGTPCHI,encoding the initial enzymes necessary for folate synthesis,positively regulate folate accumulation in knockout mutants of both japonica and indica rice backgrounds.The folate content in the low-folate japonica variety was slightly increased by the expression of the indica alleles driven by the endosperm-specific promoter.We further obtained co-expression lines by stacking OsADCS and OsGTPCHI genes;the folate accumulation in brown rice and polished rice reached 5.65μg/g and 2.95μg/g,respectively,representing 37.9-fold and 26.5-fold increases compared with the wild type.Transcriptomic analysis of rice grains from six transgenic lines showed that folate changes affected biological pathways involved in the synthesis and metabolism of rice seed storage substances,while the expression of other folate synthesis genes was weakly regulated.In addition,we identified Aus rice as a high-folate germplasm carrying superior haplotypes of OsADCS and OsGTPCHI through natural variation.This study provides an alternative and effective complementary strategy for rice biofortification,promoting the rational combination of metabolic engineering and conventional breeding to breed high-folate varieties.展开更多
Oral expression skills play an essential role in the development of EFL students’language abilities,and how to improve EFL students’oral expression skills is an essential and challenging task.This study adopts a qua...Oral expression skills play an essential role in the development of EFL students’language abilities,and how to improve EFL students’oral expression skills is an essential and challenging task.This study adopts a quasi-experimental research method to carry out the research and proposes an AI-based reflective dialogue model.Based on this,an analysis of the impact brought by this model on EFL students’oral expression performance and learning anxiety levels.The results show that students in the experimental group have significantly higher oral expression performance than those in the control group in the three dimensions of grammatical accuracy,expressive fluency,and word accuracy.In addition,the students in the experimental group produced facilitated anxiety after using the AI-based reflective dialogue model for oral expression learning,which prompted the students to learn more diligently.展开更多
Objective INF2 is a member of the formins family.Abnormal expression and regulation of INF2 have been associated with the progression of various tumors,but the expression and role of INF2 in hepatocellular carcinoma(H...Objective INF2 is a member of the formins family.Abnormal expression and regulation of INF2 have been associated with the progression of various tumors,but the expression and role of INF2 in hepatocellular carcinoma(HCC)remain unclear.HCC is a highly lethal malignant tumor.Given the limitations of traditional treatments,this study explored the expression level,clinical value and potential mechanism of INF2 in HCC in order to seek new therapeutic targets.Methods In this study,we used public databases to analyze the expression of INF2 in pan-cancer and HCC,as well as the impact of INF2 expression levels on HCC prognosis.Quantitative real time polymerase chain reaction(RT-qPCR),Western blot,and immunohistochemistry were used to detect the expression level of INF2 in liver cancer cells and human HCC tissues.The correlation between INF2 expression and clinical pathological features was analyzed using public databases and clinical data of human HCC samples.Subsequently,the effects of INF2 expression on the biological function and Drp1 phosphorylation of liver cancer cells were elucidated through in vitro and in vivo experiments.Finally,the predictive value and potential mechanism of INF2 in HCC were further analyzed through database and immunohistochemical experiments.Results INF2 is aberrantly high expression in HCC samples and the high expression of INF2 is correlated with overall survival,liver cirrhosis and pathological differentiation of HCC patients.The expression level of INF2 has certain diagnostic value in predicting the prognosis and pathological differentiation of HCC.In vivo and in vitro HCC models,upregulated expression of INF2 triggers the proliferation and migration of the HCC cell,while knockdown of INF2 could counteract this effect.INF2 in liver cancer cells may affect mitochondrial division by inducing Drp1 phosphorylation and mediate immune escape by up-regulating PD-L1 expression,thus promoting tumor progression.Conclusion INF2 is highly expressed in HCC and is associated with poor prognosis.High expression of INF2 may promote HCC progression by inducing Drp1 phosphorylation and up-regulation of PD-L1 expression,and targeting INF2 may be beneficial for HCC patients with high expression of INF2.展开更多
The xylitol dehydrogenase(XDH)is a crucial enzyme involved in the xylose utilization in pentose⁃catabolizing yeasts and fungi.In addition to producing xylulose,XDH can also be employed to develop a biosensor for monit...The xylitol dehydrogenase(XDH)is a crucial enzyme involved in the xylose utilization in pentose⁃catabolizing yeasts and fungi.In addition to producing xylulose,XDH can also be employed to develop a biosensor for monitoring xylitol concentration.In this study,the gene encoding the thermophilic fungus Talaromyces emersonii XDH(TeXDH)was heterologously expressed in Escherichia coli BL21(DE3)at 16℃in the soluble form.Recombinant TeXDH with high purity was purified by using a Ni⁃NTA affinity column.Size⁃exclusion chromatography and SDS⁃PAGE analysis demonstrated that the puri⁃fied recombinant TeXDH exists as a native trimer with a molecular mass of approximately 116 kD,and is composed of three identical subunits,each with a molecular weight of around 39 kD.The TeXDH strictly preferred NAD^(+)as a coenzyme to NADP^(+).The optimal temperature and pH of the TeXDH were 40℃and 10.0,respectively.After EDTA treatment,the enzyme activity of TeXDH decreased to 43.26%of the initial enzyme activity,while the divalent metal ions Mg^(2+)or Ca^(2+)could recover the enzyme activity of TeXDH,reaching 103.32%and 110.69%of the initial enzyme activity,respectively,making them the optimal divalent metal ion cofactors for TeXDH enzyme.However,the divalent metal ions of Mn^(2+),Ni^(2+),Cu^(2+),Zn^(2+),Co^(2+),and Cd^(2+)significantly inhibited the activity of TeXDH.ICP⁃MS and molecular doc⁃king studies revealed that 1 mol/L of TeXDH bound 2 mol/L Zn^(2+)ions and 1 mol/L Mg^(2+)ion.Further⁃more,TeXDH exhibited a high specificity for xylitol,laying the foundation for the development of future xylitol biosensors.展开更多
Based on the requirements of local high-quality economic development and addressing the critical task of transformation and upgrading in the tea industry,this paper systematically discusses the necessity and feasibili...Based on the requirements of local high-quality economic development and addressing the critical task of transformation and upgrading in the tea industry,this paper systematically discusses the necessity and feasibility of constructing an optimal industrialization operation system driven by the dual wheels of"branding+standardization".The article first clarifies the connotation of high-quality development and the synergistic mechanism between branding and standardization.It then analyzes the current situation and bottlenecks of China's tea industry development.Subsequently,it proposes a dual-wheel drive strategy where branding enhances value and standardization guarantees quality,and designs a systematic implementation plan involving industrial chain synergy optimization and integrated support from government,industry,academia,research,and application.On this basis,strategies and suggestions are proposed,encompassing the starting point,standard focal points,key effort areas,innovation points,and target achievement points.The aim is to promote the tea industry to break through homogeneous competition,achieve value ascent,and provide important industrial support for regional high-quality development through the construction of the aforementioned system.展开更多
With the rapid development of China's insurance market,the high-quality development of the insurance market is faced with many different challenges and various problems.This paper aims to deeply analyze the actual...With the rapid development of China's insurance market,the high-quality development of the insurance market is faced with many different challenges and various problems.This paper aims to deeply analyze the actual problems in China's insurance market and put forward supporting countermeasures.First,by understanding the background of highquality insurance development,we can explain the research significance of this paper in combination with the existing national policies.Secondly,by summarizing the current situation of high-quality insurance development,the paper puts forward the shortage of insurance talents in the expansion of insurance scale,the continuous improvement of insurance density and depth,and the growth of insurance compensation in the development of high-quality insurance.Finally,from the perspective of differentiated customized insurance products,training professionals,and providing financial subsidies,we will put forward the corresponding suggestions for the problem and look forward to the future development prospects.展开更多
DNA microarray technology is an extremely effective technique for studying gene expression patterns in cells, and the main challenge currently faced by this technology is how to analyze the large amount of gene expres...DNA microarray technology is an extremely effective technique for studying gene expression patterns in cells, and the main challenge currently faced by this technology is how to analyze the large amount of gene expression data generated. To address this, this paper employs a mixed-effects model to analyze gene expression data. In terms of data selection, 1176 genes from the white mouse gene expression dataset under two experimental conditions were chosen, setting up two conditions: pneumococcal infection and no infection, and constructing a mixed-effects model. After preprocessing the gene chip information, the data were imported into the model, preliminary results were calculated, and permutation tests were performed to biologically validate the preliminary results using GSEA. The final dataset consists of 20 groups of gene expression data from pneumococcal infection, which categorizes functionally related genes based on the similarity of their expression profiles, facilitating the study of genes with unknown functions.展开更多
High-quality clinical evidence is the basis of evidence-based medical practice.Acupuncture is the most widely used complementary and alternative medicine in the world.However,the improvement in the quality of acupunct...High-quality clinical evidence is the basis of evidence-based medical practice.Acupuncture is the most widely used complementary and alternative medicine in the world.However,the improvement in the quality of acupuncture clinical research does not match the rapid increase in the number of acupuncture clinical research in recent years.At present,the number of high-quality acupuncture clinical research in the world remains low.Taking the trial of acupuncture for chronic spontaneous urticaria published in Annals of Internal Medicine as an example,this paper discusses the factors that contribute to producing high-quality clinical evidence for acupuncture from the aspects of selecting the topic of study,formulating the study method,designing the study plan,controlling the study process,writing the study report,and selecting the journal in the process of before,during,and after the research.It emphasizes the necessity to start from the benefit of global people's health,take practical clinical problems as guidance,cleverly choose the research entry point,and conduct high-quality research with clinical value.After the accomplishment of the study,the results must be faithfully described,the scientific conclusions accurately sublimated,and appropriate scientific journals selected for publication.展开更多
Objective:To explore the effects of high-quality nursing intervention on negative emotions and quality of life in gynecological patients after laparoscopy.Methods:A total of 132 gynecological patients after laparoscop...Objective:To explore the effects of high-quality nursing intervention on negative emotions and quality of life in gynecological patients after laparoscopy.Methods:A total of 132 gynecological patients after laparoscopy were randomly divided into an observation group(n=66)and a control group(n=66)in a prospective study.The con-trol group received routine nursing care,while the observation group received high-quality nursing intervention.Anxiety,depression,quality of life,postoperative pain,self-care ability,and patient satisfaction were compared between the two groups.Results:The Self-Rating Anxiety Scale and Self-Rating Depression Scale scores were sig-nificantly lower in the observation group compared to the control group(both P<0.001).Pain scores at 6,24,48,and 72 hours post-surgery were also lower in the observation group(all P<0.001).The observation group showed significantly higher scores in physical function,general health,social function,emotional role,and mental health(all P<0.001).Furthermore,the observation group demonstrated better self-care skills,self-concept,self-care responsibility,and health knowledge(all P<0.001).Nursing satisfaction during hospitalization was significantly higher in the observation group than in the control group(P<0.05).Conclusion:High-quality nursing intervention is effective in improving depression,anxiety,postoperative pain,and quality of life in gynecological patients after laparoscopy.It also enhances self-care ability and patient satisfaction,making it worthy of clinical promotion and application.展开更多
Neuronal activity,synaptic transmission,and molecular changes in the basolateral amygdala play critical roles in fear memory.Cylindromatosis(CYLD)is a deubiquitinase that negatively regulates the nuclear factor kappa-...Neuronal activity,synaptic transmission,and molecular changes in the basolateral amygdala play critical roles in fear memory.Cylindromatosis(CYLD)is a deubiquitinase that negatively regulates the nuclear factor kappa-B pathway.CYLD is well studied in non-neuronal cells,yet underinvestigated in the brain,where it is highly expressed.Emerging studies have shown involvement of CYLD in the remodeling of glutamatergic synapses,neuroinflammation,fear memory,and anxiety-and autism-like behaviors.However,the precise role of CYLD in glutamatergic neurons is largely unknown.Here,we first proposed involvement of CYLD in cued fear expression.We next constructed transgenic model mice with specific deletion of Cyld from glutamatergic neurons.Our results show that glutamatergic CYLD deficiency exaggerated the expression of cued fear in only male mice.Further,loss of CYLD in glutamatergic neurons resulted in enhanced neuronal activation,impaired excitatory synaptic transmission,and altered levels of glutamate receptors accompanied by over-activation of microglia in the basolateral amygdala of male mice.Altogether,our study suggests a critical role of glutamatergic CYLD in maintaining normal neuronal,synaptic,and microglial activation.This may contribute,at least in part,to cued fear expression.展开更多
Songji Ancient Town in Yongchuan District,Chongqing,is a famous historical and cultural town in China and a national AAAA-level tourist attraction.In recent years,combining its unique historical and cultural heritage,...Songji Ancient Town in Yongchuan District,Chongqing,is a famous historical and cultural town in China and a national AAAA-level tourist attraction.In recent years,combining its unique historical and cultural heritage,the scenic area has developed research travel products themed on intangible cultural heritage and red tourism,attracting students from across the country to experience it.On the other hand,in the context of the deepening of the“double reduction”policy and the concept of a“high-quality education system,”the educational connotation of Songji Ancient Town’s research products is constantly enriching.Based on this,this article will combine the RMP theory to explore strategies for improving satisfaction with Yongchuan District’s“Songji Ancient Town Research Products”under a high-quality education system,to promote the development of the scenic area’s research experience projects and overall tourism service levels.展开更多
Highly crystalline perovskite absorbers with low defect-state densities minimizing nonradiative recombination losses are a critical prerequisite for fabricating state-of-the-art photovoltaics.Here,we use a tartaric ac...Highly crystalline perovskite absorbers with low defect-state densities minimizing nonradiative recombination losses are a critical prerequisite for fabricating state-of-the-art photovoltaics.Here,we use a tartaric acid(TA)molecule with two carboxyl and two hydroxyl groups as an additive to improve the performance and stability of the device simultaneously.The strong carboxyl-Pb2+coordination slows nucleation kinetics and passivates Pb-related traps,whereas hydroxyl-I-hydrogen bonding can modulate grain growth and stabilize the lattice structure,collectively enabling low-defect-density and high-quality perovskite films.Besides,we also conducted quantitively loss analysis and confirmed that the TA addition effectively reduces trap-assisted non-radiative recombination.Consequently,the champion efficiency of the n-i-p structure is up to 24.77% with outstanding operational and humidity stability.Remarkably,in the triple-cation perovskite system,the incorporation of the TA additive similarly enabled the fabrication of high-quality films,ultimately yielding a p-i-n configuration with a champion efficiency of 26.11%.展开更多
Under the background of"Digital Commerce for Rural Vitalization",rural E-commerce has experienced rapid development.However,agricultural products like strawberries,often produced by small-scale,fragmented,an...Under the background of"Digital Commerce for Rural Vitalization",rural E-commerce has experienced rapid development.However,agricultural products like strawberries,often produced by small-scale,fragmented,and less competitive individual farmers,struggle to meet the compliance and scalability demands of E-commerce,thereby constraining high-quality local economic development.Aiming to address this issue,this paper,guided by relevant policies and strategies,employs case analysis and logical deduction to explore the industrialization path of the"Cooperative+E-commerce"model for the strawberry industry.The research finds that by optimizing the cooperative's organizational structure,implementing multi-channel E-commerce strategies,upgrading the supply chain(including cold chain and quality traceability),and engaging in collaborative brand building,a robust industrial system can be formed.Supplemented by benefit evaluation,policy support,and regulatory oversight,this system can effectively bridge small-scale production with the broader market.This study concludes that this pathway can enhance the added value of the strawberry industry,increase farmer incomes,and provide practical insights for promoting high-quality local economic development.展开更多
[Objectives]This study was conducted to investigate the impact of soybean intercropping on soil fertility in the planting areas of high-quality peach orchards in Shenxi Town,Zunyi City,Guizhou Province.[Methods]Soil s...[Objectives]This study was conducted to investigate the impact of soybean intercropping on soil fertility in the planting areas of high-quality peach orchards in Shenxi Town,Zunyi City,Guizhou Province.[Methods]Soil samples were collected during the seedling,growth,and maturity stages of soybeans from both non-intercropped soil(control check group,CK)and soybean-intercropped soil(Qiandou 11,experimental group,EK).Analysis was conducted on soil fertility properties,including alkali-hydrolyzable N,available P,and available K.[Results]The impact of soybean intercropping on soil physicochemical properties was primarily manifested as the increased levels of alkali-hydrolyzable N and available K and the decreased level of available P in the soil during the seedling,growing,and maturity stages.Overall,this approach optimized and enhanced soil fertility in the peach orchards,providing a viable pathway for soil improvement in peach orchards located in karst areas.[Conclusions]The findings of this study provide a scientific basis for the high-quality development of high-quality peach orchards.展开更多
The high-quality development of grassroots teaching organizations in universities is crucial to improving the quality of higher education.From the perspective of dual-track drive,this paper deeply analyzes the synerge...The high-quality development of grassroots teaching organizations in universities is crucial to improving the quality of higher education.From the perspective of dual-track drive,this paper deeply analyzes the synergetic evolution relationship between institutions and teachers in the development of grassroots teaching organizations in universities.At present,the development of grassroots teaching organizations in universities is faced with such dilemmas as lagging institutional supply,insufficient motivation for teachers’development,and lack of synergy mechanisms.These interwoven problems have formed systemic obstacles restricting high-quality development,which urgently need in-depth analysis and resolution.Currently,only from the perspective of synergistic promotion of institutions and teachers and by constructing a systematic implementation framework can the existing problems be effectively solved.Through three dimensions-goal guidance,resource guarantee,and mechanism optimization-this paper refines nine specific measures,aiming to break the barriers between institutional development and teachers’development,form a joint force,provide theoretical support and practical paths for improving the efficiency of grassroots teaching organizations,promote the overall improvement of education and teaching quality,enhance the quality of talent training in universities,and advance the in-depth development of education and teaching reform.展开更多
Integrated urban-rural development is an inevitable choice for promoting Chinese modernization.High-quality integrated urban-rural development is essentially a practical response to the current multi-factor and multi-...Integrated urban-rural development is an inevitable choice for promoting Chinese modernization.High-quality integrated urban-rural development is essentially a practical response to the current multi-factor and multi-sectoral challenges in urban-rural relations,and plays an important role in implementing the new development philosophy,which promotes Chinese modernization.The theoretical logic supporting the function it plays in enabling Chinese modernization lies in the following aspects:it leverages the free flow of population and resources between urban and rural areas and thus facilitates the balanced development of China’s modernization;it advances the common prosperity of all people through its new type of urban-rural and industrial-agricultural relations;it fosters the coordinated development of the material and spiritual civilizations through the integration of urban and rural cultures;it promotes steering the modernization toward green development through the joint building and managing of the overall urban-rural ecosystem;and it drives modernization in a harmonious and stable direction through integrated urban-rural development.Currently,integrated urban-rural development still faces challenges such as restrictions on the two-way flow of factors,the low efficiency of industrial coordination and support,unequal access to public service facilities,and the insufficient use of land.Therefore,based on the strategic framework of urban-rural integrated development,it is necessary to continuously promote Chinese modernization through high-quality integrated urban-rural development from the perspectives of deepening institutional coordination,expanding the application of digital intelligence in urban and rural industries,optimizing public services,and improving land management.展开更多
In the current situation of decelerating economic expansion,examining the digital economy(DE)as a novel economic model is beneficial for the local economy’s sustainable and high-quality development(HQD).We analyzed p...In the current situation of decelerating economic expansion,examining the digital economy(DE)as a novel economic model is beneficial for the local economy’s sustainable and high-quality development(HQD).We analyzed panel data from the Yellow River(YR)region from 2013 to 2021 and discovered notable spatial variances in the composite index and coupling coordination of the two systems.Specifically,the downstream region exhibited the highest coupling coordination,while the upstream region had the lowest.We identified that favorable factors such as economic development,innovation,industrial upgrading,and government intervention can bolster the coupling.Our findings provide a valuable framework for promoting DE and HQD in the YR region.展开更多
基金supported by Central Public-interest Scientific Institution Basal Research Fund(CATAS-Nos.1630152023007,1630152023011,1630152023012,1630152023013)the National Natural Science Foundation of China(Grant No.32071805).
文摘Coconut(Cocos nucifera L.),a major oil and fruit crop of the Arecaceae family,is extensively cultivated across the Asia—Pacific region.Despite its agricultural importance,genome assembly in coconut remains challenging due to its large genome size and high proportion of repetitive sequences.Allele-specific expression(ASE)plays a key role in regulating plant development and evolution,yet research on ASE in coconut is limited(Shao et al.,2019;Li et al.,2021;Zhang et al.,2021;Hu et al.,2022).Among phenotypic traits,fruit color is especially important as an indicator of maturity,guiding harvest timing and post-harvest processes(Kapoor et al.,2022).While prior studies have explored various coconut traits such as salt tolerance,fiber content,and plant height(Wang et al.,2021;Yang et al.,2021),investigations into ASE and fruit color remain scarce.
基金supported by China Academy of Railway Sciences Corporation Limited(No.2021YJ127).
文摘Artificial intelligence,such as deep learning technology,has advanced the study of facial expression recognition since facial expression carries rich emotional information and is significant for many naturalistic situations.To pursue a high facial expression recognition accuracy,the network model of deep learning is generally designed to be very deep while the model’s real-time performance is typically constrained and limited.With MobileNetV3,a lightweight model with a good accuracy,a further study is conducted by adding a basic ResNet module to each of its existing modules and an SSH(Single Stage Headless Face Detector)context module to expand the model’s perceptual field.In this article,the enhanced model named Res-MobileNetV3,could alleviate the subpar of real-time performance and compress the size of large network models,which can process information at a rate of up to 33 frames per second.Although the improved model has been verified to be slightly inferior to the current state-of-the-art method in aspect of accuracy rate on the publically available face expression datasets,it can bring a good balance on accuracy,real-time performance,model size and model complexity in practical applications.
基金supported by the Central Public-Interest Scientific Institution Basal Research Fund,China(Grant No.CPSIBRF-CNRRI-202403)。
文摘Rice is a poor source of folate,an essential micronutrient for the body.Biofortification offers an effective way to enhance the folate content of rice and alleviate folate deficiencies in humans.In this study,we confirmed that OsADCS and OsGTPCHI,encoding the initial enzymes necessary for folate synthesis,positively regulate folate accumulation in knockout mutants of both japonica and indica rice backgrounds.The folate content in the low-folate japonica variety was slightly increased by the expression of the indica alleles driven by the endosperm-specific promoter.We further obtained co-expression lines by stacking OsADCS and OsGTPCHI genes;the folate accumulation in brown rice and polished rice reached 5.65μg/g and 2.95μg/g,respectively,representing 37.9-fold and 26.5-fold increases compared with the wild type.Transcriptomic analysis of rice grains from six transgenic lines showed that folate changes affected biological pathways involved in the synthesis and metabolism of rice seed storage substances,while the expression of other folate synthesis genes was weakly regulated.In addition,we identified Aus rice as a high-folate germplasm carrying superior haplotypes of OsADCS and OsGTPCHI through natural variation.This study provides an alternative and effective complementary strategy for rice biofortification,promoting the rational combination of metabolic engineering and conventional breeding to breed high-folate varieties.
基金2024 Provincial Teaching Reform Program for Graduate Students in the Second Batch of the 14th Five-Year Plan of Zhejiang Provincial Office of Education:Innovation and Practice of“Six Synergistic”Graduate Teaching Guided by Educator’s Spirit(No.JGCG2024406)Key Project of Zhejiang Provincial Education Science Planning:Research on an interdisciplinary teaching model to promote students’computational thinking from multiple analytical perspectives[No.2025SB103].
文摘Oral expression skills play an essential role in the development of EFL students’language abilities,and how to improve EFL students’oral expression skills is an essential and challenging task.This study adopts a quasi-experimental research method to carry out the research and proposes an AI-based reflective dialogue model.Based on this,an analysis of the impact brought by this model on EFL students’oral expression performance and learning anxiety levels.The results show that students in the experimental group have significantly higher oral expression performance than those in the control group in the three dimensions of grammatical accuracy,expressive fluency,and word accuracy.In addition,the students in the experimental group produced facilitated anxiety after using the AI-based reflective dialogue model for oral expression learning,which prompted the students to learn more diligently.
文摘Objective INF2 is a member of the formins family.Abnormal expression and regulation of INF2 have been associated with the progression of various tumors,but the expression and role of INF2 in hepatocellular carcinoma(HCC)remain unclear.HCC is a highly lethal malignant tumor.Given the limitations of traditional treatments,this study explored the expression level,clinical value and potential mechanism of INF2 in HCC in order to seek new therapeutic targets.Methods In this study,we used public databases to analyze the expression of INF2 in pan-cancer and HCC,as well as the impact of INF2 expression levels on HCC prognosis.Quantitative real time polymerase chain reaction(RT-qPCR),Western blot,and immunohistochemistry were used to detect the expression level of INF2 in liver cancer cells and human HCC tissues.The correlation between INF2 expression and clinical pathological features was analyzed using public databases and clinical data of human HCC samples.Subsequently,the effects of INF2 expression on the biological function and Drp1 phosphorylation of liver cancer cells were elucidated through in vitro and in vivo experiments.Finally,the predictive value and potential mechanism of INF2 in HCC were further analyzed through database and immunohistochemical experiments.Results INF2 is aberrantly high expression in HCC samples and the high expression of INF2 is correlated with overall survival,liver cirrhosis and pathological differentiation of HCC patients.The expression level of INF2 has certain diagnostic value in predicting the prognosis and pathological differentiation of HCC.In vivo and in vitro HCC models,upregulated expression of INF2 triggers the proliferation and migration of the HCC cell,while knockdown of INF2 could counteract this effect.INF2 in liver cancer cells may affect mitochondrial division by inducing Drp1 phosphorylation and mediate immune escape by up-regulating PD-L1 expression,thus promoting tumor progression.Conclusion INF2 is highly expressed in HCC and is associated with poor prognosis.High expression of INF2 may promote HCC progression by inducing Drp1 phosphorylation and up-regulation of PD-L1 expression,and targeting INF2 may be beneficial for HCC patients with high expression of INF2.
基金湖南省教育厅基金优秀青年项目(No.22B0482)湖南科技大学博士启动基金(No.E51992 and E51993)资助。
文摘The xylitol dehydrogenase(XDH)is a crucial enzyme involved in the xylose utilization in pentose⁃catabolizing yeasts and fungi.In addition to producing xylulose,XDH can also be employed to develop a biosensor for monitoring xylitol concentration.In this study,the gene encoding the thermophilic fungus Talaromyces emersonii XDH(TeXDH)was heterologously expressed in Escherichia coli BL21(DE3)at 16℃in the soluble form.Recombinant TeXDH with high purity was purified by using a Ni⁃NTA affinity column.Size⁃exclusion chromatography and SDS⁃PAGE analysis demonstrated that the puri⁃fied recombinant TeXDH exists as a native trimer with a molecular mass of approximately 116 kD,and is composed of three identical subunits,each with a molecular weight of around 39 kD.The TeXDH strictly preferred NAD^(+)as a coenzyme to NADP^(+).The optimal temperature and pH of the TeXDH were 40℃and 10.0,respectively.After EDTA treatment,the enzyme activity of TeXDH decreased to 43.26%of the initial enzyme activity,while the divalent metal ions Mg^(2+)or Ca^(2+)could recover the enzyme activity of TeXDH,reaching 103.32%and 110.69%of the initial enzyme activity,respectively,making them the optimal divalent metal ion cofactors for TeXDH enzyme.However,the divalent metal ions of Mn^(2+),Ni^(2+),Cu^(2+),Zn^(2+),Co^(2+),and Cd^(2+)significantly inhibited the activity of TeXDH.ICP⁃MS and molecular doc⁃king studies revealed that 1 mol/L of TeXDH bound 2 mol/L Zn^(2+)ions and 1 mol/L Mg^(2+)ion.Further⁃more,TeXDH exhibited a high specificity for xylitol,laying the foundation for the development of future xylitol biosensors.
基金Supported by General Project of Philosophy and Social Sciences Research in Universities of Jiangsu Province,2024(2024SJYB1650).
文摘Based on the requirements of local high-quality economic development and addressing the critical task of transformation and upgrading in the tea industry,this paper systematically discusses the necessity and feasibility of constructing an optimal industrialization operation system driven by the dual wheels of"branding+standardization".The article first clarifies the connotation of high-quality development and the synergistic mechanism between branding and standardization.It then analyzes the current situation and bottlenecks of China's tea industry development.Subsequently,it proposes a dual-wheel drive strategy where branding enhances value and standardization guarantees quality,and designs a systematic implementation plan involving industrial chain synergy optimization and integrated support from government,industry,academia,research,and application.On this basis,strategies and suggestions are proposed,encompassing the starting point,standard focal points,key effort areas,innovation points,and target achievement points.The aim is to promote the tea industry to break through homogeneous competition,achieve value ascent,and provide important industrial support for regional high-quality development through the construction of the aforementioned system.
文摘With the rapid development of China's insurance market,the high-quality development of the insurance market is faced with many different challenges and various problems.This paper aims to deeply analyze the actual problems in China's insurance market and put forward supporting countermeasures.First,by understanding the background of highquality insurance development,we can explain the research significance of this paper in combination with the existing national policies.Secondly,by summarizing the current situation of high-quality insurance development,the paper puts forward the shortage of insurance talents in the expansion of insurance scale,the continuous improvement of insurance density and depth,and the growth of insurance compensation in the development of high-quality insurance.Finally,from the perspective of differentiated customized insurance products,training professionals,and providing financial subsidies,we will put forward the corresponding suggestions for the problem and look forward to the future development prospects.
文摘DNA microarray technology is an extremely effective technique for studying gene expression patterns in cells, and the main challenge currently faced by this technology is how to analyze the large amount of gene expression data generated. To address this, this paper employs a mixed-effects model to analyze gene expression data. In terms of data selection, 1176 genes from the white mouse gene expression dataset under two experimental conditions were chosen, setting up two conditions: pneumococcal infection and no infection, and constructing a mixed-effects model. After preprocessing the gene chip information, the data were imported into the model, preliminary results were calculated, and permutation tests were performed to biologically validate the preliminary results using GSEA. The final dataset consists of 20 groups of gene expression data from pneumococcal infection, which categorizes functionally related genes based on the similarity of their expression profiles, facilitating the study of genes with unknown functions.
基金Supported by Sichuan Province science and technology education joint fund project:2024NSFSC1976Science and Technology Research Project of Sichuan Ad-ministration of Traditional Chinese Medicine:2021MS320.
文摘High-quality clinical evidence is the basis of evidence-based medical practice.Acupuncture is the most widely used complementary and alternative medicine in the world.However,the improvement in the quality of acupuncture clinical research does not match the rapid increase in the number of acupuncture clinical research in recent years.At present,the number of high-quality acupuncture clinical research in the world remains low.Taking the trial of acupuncture for chronic spontaneous urticaria published in Annals of Internal Medicine as an example,this paper discusses the factors that contribute to producing high-quality clinical evidence for acupuncture from the aspects of selecting the topic of study,formulating the study method,designing the study plan,controlling the study process,writing the study report,and selecting the journal in the process of before,during,and after the research.It emphasizes the necessity to start from the benefit of global people's health,take practical clinical problems as guidance,cleverly choose the research entry point,and conduct high-quality research with clinical value.After the accomplishment of the study,the results must be faithfully described,the scientific conclusions accurately sublimated,and appropriate scientific journals selected for publication.
文摘Objective:To explore the effects of high-quality nursing intervention on negative emotions and quality of life in gynecological patients after laparoscopy.Methods:A total of 132 gynecological patients after laparoscopy were randomly divided into an observation group(n=66)and a control group(n=66)in a prospective study.The con-trol group received routine nursing care,while the observation group received high-quality nursing intervention.Anxiety,depression,quality of life,postoperative pain,self-care ability,and patient satisfaction were compared between the two groups.Results:The Self-Rating Anxiety Scale and Self-Rating Depression Scale scores were sig-nificantly lower in the observation group compared to the control group(both P<0.001).Pain scores at 6,24,48,and 72 hours post-surgery were also lower in the observation group(all P<0.001).The observation group showed significantly higher scores in physical function,general health,social function,emotional role,and mental health(all P<0.001).Furthermore,the observation group demonstrated better self-care skills,self-concept,self-care responsibility,and health knowledge(all P<0.001).Nursing satisfaction during hospitalization was significantly higher in the observation group than in the control group(P<0.05).Conclusion:High-quality nursing intervention is effective in improving depression,anxiety,postoperative pain,and quality of life in gynecological patients after laparoscopy.It also enhances self-care ability and patient satisfaction,making it worthy of clinical promotion and application.
基金supported by the National Natural Science Foundation of China,Nos.32371065(to CL)and 32170950(to LY)the Natural Science Foundation of the Guangdong Province,No.2023A1515010899(to CL)the Science and Technology Projects in Guangzhou,Nos.2023A4J0578 and 2024A03J0180(to CW)。
文摘Neuronal activity,synaptic transmission,and molecular changes in the basolateral amygdala play critical roles in fear memory.Cylindromatosis(CYLD)is a deubiquitinase that negatively regulates the nuclear factor kappa-B pathway.CYLD is well studied in non-neuronal cells,yet underinvestigated in the brain,where it is highly expressed.Emerging studies have shown involvement of CYLD in the remodeling of glutamatergic synapses,neuroinflammation,fear memory,and anxiety-and autism-like behaviors.However,the precise role of CYLD in glutamatergic neurons is largely unknown.Here,we first proposed involvement of CYLD in cued fear expression.We next constructed transgenic model mice with specific deletion of Cyld from glutamatergic neurons.Our results show that glutamatergic CYLD deficiency exaggerated the expression of cued fear in only male mice.Further,loss of CYLD in glutamatergic neurons resulted in enhanced neuronal activation,impaired excitatory synaptic transmission,and altered levels of glutamate receptors accompanied by over-activation of microglia in the basolateral amygdala of male mice.Altogether,our study suggests a critical role of glutamatergic CYLD in maintaining normal neuronal,synaptic,and microglial activation.This may contribute,at least in part,to cued fear expression.
文摘Songji Ancient Town in Yongchuan District,Chongqing,is a famous historical and cultural town in China and a national AAAA-level tourist attraction.In recent years,combining its unique historical and cultural heritage,the scenic area has developed research travel products themed on intangible cultural heritage and red tourism,attracting students from across the country to experience it.On the other hand,in the context of the deepening of the“double reduction”policy and the concept of a“high-quality education system,”the educational connotation of Songji Ancient Town’s research products is constantly enriching.Based on this,this article will combine the RMP theory to explore strategies for improving satisfaction with Yongchuan District’s“Songji Ancient Town Research Products”under a high-quality education system,to promote the development of the scenic area’s research experience projects and overall tourism service levels.
基金funding support from the National Key Research and Development Program of China(2022YFE0137400)the National Natural Science Foundation of China(62274040)+3 种基金funding support from the National Natural Science Foundation of China(62304046)the National Key Research and Development Program of China(2022YFB2802802)the Key Laboratory of Rare Earths,Ganjiang Innovation Academy,Chinese Academy of Sciencessupport from the Shanghai Science and Technology Innovation Action Plan 2023 Special Project for Supporting Carbon Peak Carbon Neutrality Project(23DZ1200400)。
文摘Highly crystalline perovskite absorbers with low defect-state densities minimizing nonradiative recombination losses are a critical prerequisite for fabricating state-of-the-art photovoltaics.Here,we use a tartaric acid(TA)molecule with two carboxyl and two hydroxyl groups as an additive to improve the performance and stability of the device simultaneously.The strong carboxyl-Pb2+coordination slows nucleation kinetics and passivates Pb-related traps,whereas hydroxyl-I-hydrogen bonding can modulate grain growth and stabilize the lattice structure,collectively enabling low-defect-density and high-quality perovskite films.Besides,we also conducted quantitively loss analysis and confirmed that the TA addition effectively reduces trap-assisted non-radiative recombination.Consequently,the champion efficiency of the n-i-p structure is up to 24.77% with outstanding operational and humidity stability.Remarkably,in the triple-cation perovskite system,the incorporation of the TA additive similarly enabled the fabrication of high-quality films,ultimately yielding a p-i-n configuration with a champion efficiency of 26.11%.
基金General Project of Philosophy and Social Sciences Research in Universities of Jiangsu Province,2024(2024SJYB1650).
文摘Under the background of"Digital Commerce for Rural Vitalization",rural E-commerce has experienced rapid development.However,agricultural products like strawberries,often produced by small-scale,fragmented,and less competitive individual farmers,struggle to meet the compliance and scalability demands of E-commerce,thereby constraining high-quality local economic development.Aiming to address this issue,this paper,guided by relevant policies and strategies,employs case analysis and logical deduction to explore the industrialization path of the"Cooperative+E-commerce"model for the strawberry industry.The research finds that by optimizing the cooperative's organizational structure,implementing multi-channel E-commerce strategies,upgrading the supply chain(including cold chain and quality traceability),and engaging in collaborative brand building,a robust industrial system can be formed.Supplemented by benefit evaluation,policy support,and regulatory oversight,this system can effectively bridge small-scale production with the broader market.This study concludes that this pathway can enhance the added value of the strawberry industry,increase farmer incomes,and provide practical insights for promoting high-quality local economic development.
基金Supported by Undergraduate Innovation and Entrepreneurship Training Program of Guizhou Province(S2024106642161)Zunyi City and Zunyi Normal University Joint Technology Research Fund(ZSKH HZ Z(2023)159)+3 种基金Zunyi Science and Technology Support Program Project(ZSKHZC NS[2023]15)Science and Technology Commissioner Innovation and Entrepreneurship Service Training Demonstration Base of Guizhou Province(HHG2025001)Natural Science Research Project of Department of Education of Guizhou Province(QJJ[2023]043)Zunyi Science and Technology Innovation Team Project(ZKCTD008).
文摘[Objectives]This study was conducted to investigate the impact of soybean intercropping on soil fertility in the planting areas of high-quality peach orchards in Shenxi Town,Zunyi City,Guizhou Province.[Methods]Soil samples were collected during the seedling,growth,and maturity stages of soybeans from both non-intercropped soil(control check group,CK)and soybean-intercropped soil(Qiandou 11,experimental group,EK).Analysis was conducted on soil fertility properties,including alkali-hydrolyzable N,available P,and available K.[Results]The impact of soybean intercropping on soil physicochemical properties was primarily manifested as the increased levels of alkali-hydrolyzable N and available K and the decreased level of available P in the soil during the seedling,growing,and maturity stages.Overall,this approach optimized and enhanced soil fertility in the peach orchards,providing a viable pathway for soil improvement in peach orchards located in karst areas.[Conclusions]The findings of this study provide a scientific basis for the high-quality development of high-quality peach orchards.
基金Interim results of the 2024 high-quality development project at Jingjiang College,Jiangsu University,titled“Research on the Teacher Evaluation Mechanism of Independent Colleges Based on the Three-Dimensional System of Innovation-Efficiency-Impact”(2024JFYA001)Interim results of the special project“Research on the Digitalization of Textbooks in the New Era”in Jiangsu Province’s universities in 2024(2024JCSZ37)。
文摘The high-quality development of grassroots teaching organizations in universities is crucial to improving the quality of higher education.From the perspective of dual-track drive,this paper deeply analyzes the synergetic evolution relationship between institutions and teachers in the development of grassroots teaching organizations in universities.At present,the development of grassroots teaching organizations in universities is faced with such dilemmas as lagging institutional supply,insufficient motivation for teachers’development,and lack of synergy mechanisms.These interwoven problems have formed systemic obstacles restricting high-quality development,which urgently need in-depth analysis and resolution.Currently,only from the perspective of synergistic promotion of institutions and teachers and by constructing a systematic implementation framework can the existing problems be effectively solved.Through three dimensions-goal guidance,resource guarantee,and mechanism optimization-this paper refines nine specific measures,aiming to break the barriers between institutional development and teachers’development,form a joint force,provide theoretical support and practical paths for improving the efficiency of grassroots teaching organizations,promote the overall improvement of education and teaching quality,enhance the quality of talent training in universities,and advance the in-depth development of education and teaching reform.
基金the Research on the Original Contribution and Global Significance of the Concept of Community with a Shared Future for Mankind from a Historical Materialist Perspective(Project No.:20&ZD026)a major project of the National Social Science Foundation of China.
文摘Integrated urban-rural development is an inevitable choice for promoting Chinese modernization.High-quality integrated urban-rural development is essentially a practical response to the current multi-factor and multi-sectoral challenges in urban-rural relations,and plays an important role in implementing the new development philosophy,which promotes Chinese modernization.The theoretical logic supporting the function it plays in enabling Chinese modernization lies in the following aspects:it leverages the free flow of population and resources between urban and rural areas and thus facilitates the balanced development of China’s modernization;it advances the common prosperity of all people through its new type of urban-rural and industrial-agricultural relations;it fosters the coordinated development of the material and spiritual civilizations through the integration of urban and rural cultures;it promotes steering the modernization toward green development through the joint building and managing of the overall urban-rural ecosystem;and it drives modernization in a harmonious and stable direction through integrated urban-rural development.Currently,integrated urban-rural development still faces challenges such as restrictions on the two-way flow of factors,the low efficiency of industrial coordination and support,unequal access to public service facilities,and the insufficient use of land.Therefore,based on the strategic framework of urban-rural integrated development,it is necessary to continuously promote Chinese modernization through high-quality integrated urban-rural development from the perspectives of deepening institutional coordination,expanding the application of digital intelligence in urban and rural industries,optimizing public services,and improving land management.
基金supported by the National Office for Philosophy and Social Sciences(grant reference 22&ZD067).
文摘In the current situation of decelerating economic expansion,examining the digital economy(DE)as a novel economic model is beneficial for the local economy’s sustainable and high-quality development(HQD).We analyzed panel data from the Yellow River(YR)region from 2013 to 2021 and discovered notable spatial variances in the composite index and coupling coordination of the two systems.Specifically,the downstream region exhibited the highest coupling coordination,while the upstream region had the lowest.We identified that favorable factors such as economic development,innovation,industrial upgrading,and government intervention can bolster the coupling.Our findings provide a valuable framework for promoting DE and HQD in the YR region.
