The Fujian oyster(Crassostrea angulata)is an economically significant shellfish species distributed mainly along the Fujian coast,Southeast China.However,its genetic diversity and structure remain unclear.The main dis...The Fujian oyster(Crassostrea angulata)is an economically significant shellfish species distributed mainly along the Fujian coast,Southeast China.However,its genetic diversity and structure remain unclear.The main distribution area of the C.angulata is located in Fujian,South China.In total,420 C.angulata were collected from 14 natural habitats(populations)along the Fujian coast,and their genetic diversity and structure were analyzed in the mitochondrial COⅠand nuclear gene ITS2 sequences.Results reveal that all the 14 populations of C.angulata exhibited high levels of genetic diversity,with a total of 57(haplotype diversity:0.811±0.016)and 124(haplotype diversity:0.912±0.007)haplotypes revealed by COⅠand ITS2,respectively.Notably,significant intermediate level of genetic differentiations between the Ningde Zhujiang(ZJ)population(F_(ST)by COI:0.035-0.142,P<0.05;F_(ST)by ITS2:0.078-0.123,P<0.05)with other populations were observed for the first time,which is also supported by the results of molecular variance analysis(F_(CT)by COⅠ:0.105,P<0.05;F_(CT)by ITS2:0.086,P<0.05)and the clustering of the ZJ population into distinct branches in the interpopulation genetic differentiation tree.Furthermore,the evolutionary tree and haplotype network analyses do not support the formation of a clear geographical genealogical structure among these 14 populations.In addition,the population dynamics analysis suggests that the C.angulata may have undergone expansion during the third ice age of the Pleistocene.These results provide a reference for the preservation and further genetic improvement of C.angulata.展开更多
This commentary is based on the work of Cooper,Davis,and Van Vliet(2016)and the commentary focuses on what problem high-frequency trading poses.It lists key literature on high-frequency trading that is missing and poi...This commentary is based on the work of Cooper,Davis,and Van Vliet(2016)and the commentary focuses on what problem high-frequency trading poses.It lists key literature on high-frequency trading that is missing and points out that the poker analogy to defend deception in financial markets is weak and misleading.The article elaborates on the negative impact created by spoofing and quote stuffing,the two typical deceptive practices used by high-frequency traders.The recent regulations regarding high-frequency trading,in response to the“Flash Crash”of 2010,are preventive,computerized and more effective.They reflect ethical requirements to maintain fair and stable financial markets.展开更多
The Devonian Donghe Sandstone complex in North Uplift of the Tarim Basin comprises of a series of diachronous sandy intervals deposited from the Late Devonian to Early Mississippian. They are constrained by a Late Dev...The Devonian Donghe Sandstone complex in North Uplift of the Tarim Basin comprises of a series of diachronous sandy intervals deposited from the Late Devonian to Early Mississippian. They are constrained by a Late Devonian to Early Pennsylvanian 2 nd-order supersequence and can be subdivided into five 3 rd-order sequences, namely, S1, S2, S3, S4, and S5, from the oldest to youngest. Cores from four wells, 40 wireline logs, 410 thin sections, and porosity and permeability data from 639 spots from four wells were used to study the sediment provenance, build up the sequence-stratigraphic model of S5, and characterize the reservoirs at a feet scale. Detrital modes of sandstone from point counting indicate that Donghe Sandstone is directly sourced from recycled orogeny. The low content of feldspar and volcanic rock fragments suggests that Donghe Sandstone is recycled from sediment with a cratonic ultimate source. 1 D and 2 D chronostratigraphic correlation shows that at least 12 4 th-order highfrequency sequences（HFSs）, from the oldest HFS1 to the youngest HFS12, can be recognized in S5. Each HFS is characterized by a general trend of shallowing-upward facies assemblage. Sequence boundaries were defined at where regionally correlatable deep-water facies overlaying shallow-water facies. There is a general shallowing-upward trend in the S5 3 rd-order sequence, characterized by a systematically increasing proportion of shallow-water facies（foreshore and upper shoreface）, and a decreasing proportion of deep facies（offshore transition and lower shoreface）. The shallowing-upward trend within both 3 rd-and 4 th-order sequences is resulted from a combined effect of eustatic sea-level change, tectonic activity, and sediment supply. The sequence-stratigraphic model of Donghe Sandstone S5 is similar to the rift-basin sequence-stratigraphic model. Sweet spots were defined as porosity 〉15% and permeability 〉100 md intervals, and their distribution and lateral continuity were investigated. HFS is one of the primary controls on the distribution of sweet spots distribution and can be used to guide hydrocarbon exploration.展开更多
Wavelet transformation is a widely used method in high-frequency sequence stratigraphic analysis.However, the application is problematic since different wavelets always return the same sequence analysis results. To ad...Wavelet transformation is a widely used method in high-frequency sequence stratigraphic analysis.However, the application is problematic since different wavelets always return the same sequence analysis results. To address this issue, we applied five commonly used wavelets to theoretical sequence models to document some application criteria. Five gradual scale-change sequence models were simplified from the glutenite succession deposition by gravity flows to form the fining-upwards cycle sequences(FUCS) and coarsening-upwards cycle sequences(CUCS). After conducting theoretical sequence model tests, the optimal wavelet(sym4) was selected and successfully used with actual data to identify the sequence boundaries. We also proposed a new method to optimize the scale of continuous wavelet transformation(CWT) for sequence boundary determination. We found that the balloon-like marks in scalograms of db4, sym4, and coif4 wavelet determine, respectively, the fourth-order sequence boundary, the thick succession sequence boundaries in FUCS, and the thick succession sequence in FUCS and CUCS. Comparing the sequence identification results shows that the asymmetric wavelets had an advantage in high-frequency sequence boundary determination and sedimentary cycle discrimination through the amplitude trend of the coefficient, in which the sym4 wavelet is the most effective. In conclusion, the asymmetry of wavelets is the first selection principle, of which asymmetric wavelets are more sensitive to sediment deposition by flood flows. The match of the wavelet between the sequence is the second selection principle, in which the correlation of time-frequency impacts the accuracy of sequence surface localization. However, the waveform of the wavelet is a visual and abstract parameter for sequence boundary detection. The appropriate wavelet for lacustrine sequence analysis is the asymmetric wavelet with a weak number of side lobes. The depositional flows, depositional process,and autogenic are three sedimentary factors that influence the sequence analysis results.展开更多
Salience theory has been proposed as a new stock trading strategy.To assess the validity of this proposal,a complex decision trading system was constructed based on salience theory,a variational mode decomposition(VMD...Salience theory has been proposed as a new stock trading strategy.To assess the validity of this proposal,a complex decision trading system was constructed based on salience theory,a variational mode decomposition(VMD)model,a bidirectional gated recurrent unit(BiGRU)model,and high-frequency trading.The system selected 30 Chinese new energy concept stocks,ranked the stocks using salience theory,and selected the top and bottom three stocks for two portfolios.Twelve stages were established,following which the VMD and BiGRU models were applied to the predictions.The final predicted annualized returns for the high ST(salience theory value)group A(GA)and low ST group B(GB)were 194.06%and 165.88%,respectively.This finding validates the powerful utility of salience theory and deep learning to analyze the Chinese new energy market.Moreover,it explains the theoretical practicality issues that the short selling restriction is the essential reason,or even perhaps the only reason,that leads to the strength of salience theory.展开更多
Binary sequences constructed by Legendre symbols are widely used in communication and cryptography since they have many good pseudo-random properties.In this paper,we determine the 2-adic complexity of the sum sequenc...Binary sequences constructed by Legendre symbols are widely used in communication and cryptography since they have many good pseudo-random properties.In this paper,we determine the 2-adic complexity of the sum sequence of any k many Legendre sequences and show that the 2-adic complexity of the sum sequences of any k many Legendre sequences reaches the maximum by proving the case of k=2 and 3,which implies that the sum sequences can resist the attack of rational approximation algorithm.展开更多
The oil-based mud(OBM) borehole measurement environment presents significant limitations on the application of existing electrical logging instruments in high-resistance formations. In this paper, we propose a novel l...The oil-based mud(OBM) borehole measurement environment presents significant limitations on the application of existing electrical logging instruments in high-resistance formations. In this paper, we propose a novel logging method for detection of high-resistance formations in OBM using highfrequency electrodes. The method addresses the issue of shallow depth of investigation(DOI) in existing electrical logging instruments, while simultaneously ensuring the vertical resolution. Based on the principle of current continuity, the total impedance of the loop is obtained by equating the measurement loop to the series form of a capacitively coupled circuit. and its validity is verified in a homogeneous formation model and a radial two-layer formation model with a mud standoff. Then, the instrument operating frequency and electrode system parameters were preferentially determined by numerical simulation, and the effect of mud gap on impedance measurement was investigated. Subsequently, the DOI of the instrument was investigated utilizing the pseudo-geometric factor defined by the real part of impedance. It was determined that the detection depth of the instrument is 8.74 cm, while the effective vertical resolution was not less than 2 cm. Finally, a focused high-frequency electrode-type instrument was designed by introducing a pair of focused electrodes, which effectively enhanced the DOI of the instrument and was successfully deployed in the Oklahoma formation model. The simulation results demonstrate that the novel method can achieve a detection depth of 17.40 cm in highly-resistive formations drilling with OBM, which is approximately twice the depth of detection of the existing oil-based mud microimager instruments. Furthermore, its effective vertical resolution remains at or above 2 cm,which is comparable to the resolution of the existing OBM electrical logging instrument.展开更多
Objective:To analyze the significance of high-frequency ultrasound in differentiating benign and malignant breast micronodules.Methods:Eighty-five patients with breast micronodules admitted for diagnosis between Octob...Objective:To analyze the significance of high-frequency ultrasound in differentiating benign and malignant breast micronodules.Methods:Eighty-five patients with breast micronodules admitted for diagnosis between October 2022 and October 2024 were selected for high-frequency ultrasound diagnosis.The diagnostic efficacy of high-frequency ultrasound was evaluated by comparing it with the results of surgical pathology.Results:High-frequency ultrasound detected 50 benign nodules,primarily breast fibroadenomas,and 35 malignant nodules,mainly breast ductal carcinoma in situ.Based on surgical pathology results,the diagnostic accuracy of high-frequency ultrasound was 96.47%,specificity was 97.96%,and sensitivity was 94.44%.In high-frequency ultrasound diagnosis,the proportion of grade III and IV blood flow in malignant nodules was higher than that in benign nodules,while the proportion of regular shape and clear margins was lower.The proportion of microcalcifications and posterior echo attenuation was higher in malignant nodules,and the resistance index(RI)and peak blood flow velocity were lower than those in benign nodules(P<0.05).Conclusion:High-frequency ultrasound can effectively differentiate benign and malignant breast micronodules,determine specific nodule types,and exhibits high diagnostic accuracy and sensitivity.Additionally,benign and malignant nodules can be differentiated based on the grading of blood flow signals,sonographic features,and blood flow velocity,providing reasonable guidance for subsequent treatment plans.展开更多
China has a long history of coal mining,among which open-pit coal mines have a large number of small coal mine goafs underground.The distribution,shape,structure and other characteristics of goafs are isolated and dis...China has a long history of coal mining,among which open-pit coal mines have a large number of small coal mine goafs underground.The distribution,shape,structure and other characteristics of goafs are isolated and discontinuous,and there is no definite geological law to follow,which seriously threatens the safety of coal mine production and personnel life.Conventional ground geophysical methods have low accuracy in detecting goaf areas affected by mechanical interference from open-pit mines,especially for waterless goaf areas,which cannot be detected by existing methods.This article proposes the use of high-frequency electromagnetic waves for goaf detection.The feasibility of using drilling radar to detect goaf was theoretically analyzed,and a goaf detection model was established.The response characteristics of different fillers in the goaf under different frequencies of high-frequency electromagnetic waves were simulated and analyzed.In a certain open-pit mine in Inner Mongolia,100MHz high-frequency electromagnetic waves were used to detect the goaf through directional drilling on the ground.After detection,excavation verification was carried out,and the location of one goaf detected was verified.The results of engineering practice show that the application of high-frequency electromagnetic waves in goaf detection expands the detection radius of boreholes,has the advantages of high efficiency and accuracy,and has important theoretical and practical significance.展开更多
New electric power systems characterized by a high proportion of renewable energy and power electronics equipment face significant challenges due to high-frequency(HF)electromagnetic interference from the high-speed s...New electric power systems characterized by a high proportion of renewable energy and power electronics equipment face significant challenges due to high-frequency(HF)electromagnetic interference from the high-speed switching of power converters.To address this situation,this paper offers an in-depth review of HF interference problems and challenges originating from power electronic devices.First,the root cause of HF electromagnetic interference,i.e.,the resonant response of the parasitic parameters of the system to high-speed switching transients,is analyzed,and various scenarios of HF interference in power systems are highlighted.Next,the types of HF interference are summarized,with a focus on common-mode interference in grounding systems.This paper thoroughly reviews and compares various suppression methods for conducted HF interference.Finally,the challenges involved and suggestions for addressing emerging HF interference problems from the perspective of both power electronics equipment and power systems are discussed.This review aims to offer a structured understanding of HF interference problems and their suppression techniques for researchers and practitioners.展开更多
Single-shot ultrafast compressed imaging(UCI)is an effective tool for studying ultrafast dynamics in physics,chemistry,or material science because of its excellent high frame rate and large frame number.However,the ra...Single-shot ultrafast compressed imaging(UCI)is an effective tool for studying ultrafast dynamics in physics,chemistry,or material science because of its excellent high frame rate and large frame number.However,the random code(Rcode)used in traditional UCI will lead to low-frequency noise covering high-frequency information due to its uneven sampling interval,which is a great challenge in the fidelity of large-frame reconstruction.Here,a high-frequency enhanced compressed active photography(H-CAP)is proposed.By uniformizing the sampling interval of R-code,H-CAP capture the ultrafast process with a random uniform sampling mode.This sampling mode makes the high-frequency sampling energy dominant,which greatly suppresses the low-frequency noise blurring caused by R-code and achieves high-frequency information of image enhanced.The superior dynamic performance and large-frame reconstruction ability of H-CAP are verified by imaging optical self-focusing effect and static object,respectively.We applied H-CAP to the spatial-temporal characterization of double-pulse induced silicon surface ablation dynamics,which is performed within 220 frames in a single-shot of 300 ps.H-CAP provides a high-fidelity imaging method for observing ultrafast unrepeatable dynamic processes with large frames.展开更多
Objective:To analyze the therapeutic effect of high-frequency electrosurgical knife surgery guided by painless digestive endoscopy(PDE)in elderly patients with gastrointestinal polyps(GP).Methods:A total of 100 elderl...Objective:To analyze the therapeutic effect of high-frequency electrosurgical knife surgery guided by painless digestive endoscopy(PDE)in elderly patients with gastrointestinal polyps(GP).Methods:A total of 100 elderly GP patients admitted between June 2021 and December 2022 were selected.Patients were randomly divided into two groups:the painless group(50 cases)underwent high-frequency electrosurgical knife surgery guided by PDE,while the conventional group(50 cases)underwent the same surgery guided by traditional digestive endoscopy(DE).The total treatment efficacy,perioperative indicators,gastrointestinal hormone levels,oxidative stress(OS)markers,and complication rates were compared between the two groups.Results:The total treatment efficacy in the painless group was higher than that in the conventional group,and perioperative indicators were superior in the painless group(P<0.05).One week after treatment,the gastrointestinal hormone levels and OS-related markers in the painless group were better than those in the conventional group(P<0.05).The complication rate in the painless group was lower than in the conventional group(P<0.05).Conclusion:High-frequency electrosurgical knife surgery guided by PDE improves the effectiveness of polyp removal in elderly GP patients and accelerates postoperative recovery.It also protects gastrointestinal function,reduces postoperative OS,and ensures higher surgical safety.展开更多
Soft magnetic composites made from metallic magnetic particles with an easy magnetization plane(referred to as easy-plane metallic soft magnetic composites(SMC))are considered ideal materials for the next generation o...Soft magnetic composites made from metallic magnetic particles with an easy magnetization plane(referred to as easy-plane metallic soft magnetic composites(SMC))are considered ideal materials for the next generation of power electronic devices.This advantage is attributed to their ability to maintain high permeability at elevated frequencies.Despite these advantages,a definitive mathematical model that connects the high-frequency magnetic properties(e.g.,effective permeability)of easy-plane metallic SMCs to the intrinsic properties of the particles is still lacking.In this work,a theoretical calculation model for the effective permeability of easy-plane metallic SMCs was formulated.This model was derived from a skin effect-corrected Landau-Lifshitz-Gilbert(LLG)equation and integrated with effective medium theory incorporating inter-particle interaction.To validate the model,we prepared samples of easy-plane Y_(2)Co_(17)particle/PU SMCs with varying particle sizes and volume fractions.The experimental results showed a strong agreement with the calculated values.This research offers critical theoretical backing for the design and optimization of soft magnetic materials intended for high-frequency applications.展开更多
High-frequency pulsed(HFP)gas tungsten arc welding(GTAW)has shown excellent performance in welding of aluminum alloys in recent years,which makes itself a promisingly potential technique for part manufacturing in avia...High-frequency pulsed(HFP)gas tungsten arc welding(GTAW)has shown excellent performance in welding of aluminum alloys in recent years,which makes itself a promisingly potential technique for part manufacturing in aviation industry.However,existing researches generally focuses on the effect of a single parameter while lacks multivariable researches.Considering of the fact that gap and misalignment are inevitable in real part clamping,adaptive intelligent welding is usually used during automatic manufacturing,which means under the control of filler wire amount per length of a weld,other parameters including current,welding speed and wire feed speed during one single weld are changing according to the specific clamping situation.Therefore,the influence of specific energy input led by different welding parameters within one adaptive welding program on microstructure and mechanical property of the weld needs to be clarified.This study investigates the effect of welding heat input(ranging from 1048.3 J/mm to 825.6 J/mm within one adaptive welding program control)on the formation quality of 3.25 mm thick 6061 aluminum alloy joints fabricated by HFP-GTAW with 4043 filler wire.According to the obtained results,non-monotonic relationship between heat input and porosity,with an optimal minimum of 4.92%achieved at an intermediate heat input of 856.8 J/mm.The 21.2%decrease of energy input during welding process would reduce the average grain size in the weld center and adjacent to fusion line by 18.6%and 19.4%,respectively.The ratios between fluctuation range to minimum value in average yield and the relative ranges of yield strength and ultimate tensile strength across the tested heat inputs were 14.7%and 12.7%,respectively.The findings provide a general overview on how the microstructure and mechanical properties would fluctuate in an adaptively controlled HFP-GTAW fabricated aluminum alloy weld.展开更多
With the evolution of DC distribution networks from traditional radial topologies to more complex multi-branch structures,the number of measurement points supporting synchronous communication remains relatively limite...With the evolution of DC distribution networks from traditional radial topologies to more complex multi-branch structures,the number of measurement points supporting synchronous communication remains relatively limited.This poses challenges for conventional fault distance estimation methods,which are often tailored to simple topologies and are thus difficult to apply to large-scale,multi-node DC networks.To address this,a fault distance estimation method based on sparse measurement of high-frequency electrical quantities is proposed in this paper.First,a preliminary fault line identification model based on compressed sensing is constructed to effectively narrow the fault search range and improve localization efficiency.Then,leveraging the high-frequency impedance characteristics and the voltage-current relationship of electrical quantities,a fault distance estimation approach based on high-frequency measurements from both ends of a line is designed.This enables accurate distance estimation even when the measurement devices are not directly placed at both ends of the faulted line,overcoming the dependence on specific sensor placement inherent in traditional methods.Finally,to further enhance accuracy,an optimization model based on minimizing the high-frequency voltage error at the fault point is introduced to reduce estimation error.Simulation results demonstrate that the proposed method achieves a fault distance estimation error of less than 1%under normal conditions,and maintains good performance even under adverse scenarios.展开更多
Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macro...Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macrophages have been poorly understood and largely overlooked. However, a recent study reported that border-associated macrophages participate in stroke-induced inflammation, although many details and the underlying mechanisms remain unclear. In this study, we performed a comprehensive single-cell analysis of mouse border-associated macrophages using sequencing data obtained from the Gene Expression Omnibus(GEO) database(GSE174574 and GSE225948). Differentially expressed genes were identified, and enrichment analysis was performed to identify the transcription profile of border-associated macrophages. CellChat analysis was conducted to determine the cell communication network of border-associated macrophages. Transcription factors were predicted using the ‘pySCENIC' tool. We found that, in response to hypoxia, borderassociated macrophages underwent dynamic transcriptional changes and participated in the regulation of inflammatory-related pathways. Notably, the tumor necrosis factor pathway was activated by border-associated macrophages following ischemic stroke. The pySCENIC analysis indicated that the activity of signal transducer and activator of transcription 3(Stat3) was obviously upregulated in stroke, suggesting that Stat3 inhibition may be a promising strategy for treating border-associated macrophages-induced neuroinflammation. Finally, we constructed an animal model to investigate the effects of border-associated macrophages depletion following a stroke. Treatment with liposomes containing clodronate significantly reduced infarct volume in the animals and improved neurological scores compared with untreated animals. Taken together, our results demonstrate comprehensive changes in border-associated macrophages following a stroke, providing a theoretical basis for targeting border-associated macrophages-induced neuroinflammation in stroke treatment.展开更多
In this paper,we introduce non-abelian cohomology groups and classify the nonabelian extensions of Rota-Baxter pre-Lie algebras in terms of non-abelian cohomology groups.Next,we explore the inducibility of pairs of au...In this paper,we introduce non-abelian cohomology groups and classify the nonabelian extensions of Rota-Baxter pre-Lie algebras in terms of non-abelian cohomology groups.Next,we explore the inducibility of pairs of automorphisms and derive the analog Wells exact sequences under the circumstance of Rota-Baxter pre-Lie algebras.Finally,we discuss the inducibility problem of pairs of automorphisms about an abelian extensions of Rota-Baxter pre-Lie algebras.展开更多
The internal hotspot temperature rise prediction in nanocrystalline high-frequency transformers(nanoHFTs) is essential to ensure reliable operation. This paper presents a three-dimensional thermal network(3DTN) model ...The internal hotspot temperature rise prediction in nanocrystalline high-frequency transformers(nanoHFTs) is essential to ensure reliable operation. This paper presents a three-dimensional thermal network(3DTN) model for epoxy resin encapsulated nano HFTs, which aims to precisely predict the temperature distribution inside the transformer in combination with the finite element method(FEM). A magnetothermal bidirectional coupling 3DTN model is established by analyzing the thermal conduction between the core, windings, and epoxy resin, while also considering the convection and radiation heat transfer mechanisms on the surface of the epoxy resin. The model considers the impact of loss distribution in the core and windings on the temperature field and adopts a simplified 1/2 thermal network model to reduce computational complexity. Furthermore, the results of FEM are compared with experimental results to verify the accuracy of the 3DTN model in predicting the temperature rise of nano HFT. The results show that the 3DTN model reduces errors by an average of 5.25% over the traditional two-dimensional thermal network(2DTN) model, particularly for temperature distributions in the windings and core. This paper provides a temperature rise prediction method for the thermal design and offers a theoretical basis and engineering guidance for the optimization of their thermal management systems.展开更多
Global brain ischemia and neurological deficit are consequences of cardiac arrest that lead to high mortality.Despite advancements in resuscitation science,our limited understanding of the cellular and molecular mecha...Global brain ischemia and neurological deficit are consequences of cardiac arrest that lead to high mortality.Despite advancements in resuscitation science,our limited understanding of the cellular and molecular mechanisms underlying post-cardiac arrest brain injury have hindered the development of effective neuroprotective strategies.Previous studies primarily focused on neuronal death,potentially overlooking the contributions of non-neuronal cells and intercellular communication to the pathophysiology of cardiac arrest-induced brain injury.To address these gaps,we hypothesized that single-cell transcriptomic analysis could uncover previously unidentified cellular subpopulations,altered cell communication networks,and novel molecular mechanisms involved in post-cardiac arrest brain injury.In this study,we performed a single-cell transcriptomic analysis of the hippocampus from pigs with ventricular fibrillation-induced cardiac arrest at 6 and 24 hours following the return of spontaneous circulation,and from sham control pigs.Sequencing results revealed changes in the proportions of different cell types,suggesting post-arrest disruption in the blood-brain barrier and infiltration of neutrophils.These results were validated through western blotting,quantitative reverse transcription-polymerase chain reaction,and immunofluorescence staining.We also identified and validated a unique subcluster of activated microglia with high expression of S100A8,which increased over time following cardiac arrest.This subcluster simultaneously exhibited significant M1/M2 polarization and expressed key functional genes related to chemokines and interleukins.Additionally,we revealed the post-cardiac arrest dysfunction of oligodendrocytes and the differentiation of oligodendrocyte precursor cells into oligodendrocytes.Cell communication analysis identified enhanced post-cardiac arrest communication between neutrophils and microglia that was mediated by neutrophil-derived resistin,driving pro-inflammatory microglial polarization.Our findings provide a comprehensive single-cell map of the post-cardiac arrest hippocampus,offering potential novel targets for neuroprotection and repair following cardiac arrest.展开更多
As an important resource in data link,time slots should be strategically allocated to enhance transmission efficiency and resist eavesdropping,especially considering the tremendous increase in the number of nodes and ...As an important resource in data link,time slots should be strategically allocated to enhance transmission efficiency and resist eavesdropping,especially considering the tremendous increase in the number of nodes and diverse communication needs.It is crucial to design control sequences with robust randomness and conflict-freeness to properly address differentiated access control in data link.In this paper,we propose a hierarchical access control scheme based on control sequences to achieve high utilization of time slots and differentiated access control.A theoretical bound of the hierarchical control sequence set is derived to characterize the constraints on the parameters of the sequence set.Moreover,two classes of optimal hierarchical control sequence sets satisfying the theoretical bound are constructed,both of which enable the scheme to achieve maximum utilization of time slots.Compared with the fixed time slot allocation scheme,our scheme reduces the symbol error rate by up to 9%,which indicates a significant improvement in anti-interference and eavesdropping capabilities.展开更多
基金Supported by the National Natural Science Foundation of China(No.32172979)the Natural Science Foundation of Fujian Province(No.2021J05159)the 2023 Special Program for Promoting High-Quality Development of Marine and Fishery Industry in Fujian Province(No.PJHYF-L-2023-2)。
文摘The Fujian oyster(Crassostrea angulata)is an economically significant shellfish species distributed mainly along the Fujian coast,Southeast China.However,its genetic diversity and structure remain unclear.The main distribution area of the C.angulata is located in Fujian,South China.In total,420 C.angulata were collected from 14 natural habitats(populations)along the Fujian coast,and their genetic diversity and structure were analyzed in the mitochondrial COⅠand nuclear gene ITS2 sequences.Results reveal that all the 14 populations of C.angulata exhibited high levels of genetic diversity,with a total of 57(haplotype diversity:0.811±0.016)and 124(haplotype diversity:0.912±0.007)haplotypes revealed by COⅠand ITS2,respectively.Notably,significant intermediate level of genetic differentiations between the Ningde Zhujiang(ZJ)population(F_(ST)by COI:0.035-0.142,P<0.05;F_(ST)by ITS2:0.078-0.123,P<0.05)with other populations were observed for the first time,which is also supported by the results of molecular variance analysis(F_(CT)by COⅠ:0.105,P<0.05;F_(CT)by ITS2:0.086,P<0.05)and the clustering of the ZJ population into distinct branches in the interpopulation genetic differentiation tree.Furthermore,the evolutionary tree and haplotype network analyses do not support the formation of a clear geographical genealogical structure among these 14 populations.In addition,the population dynamics analysis suggests that the C.angulata may have undergone expansion during the third ice age of the Pleistocene.These results provide a reference for the preservation and further genetic improvement of C.angulata.
文摘This commentary is based on the work of Cooper,Davis,and Van Vliet(2016)and the commentary focuses on what problem high-frequency trading poses.It lists key literature on high-frequency trading that is missing and points out that the poker analogy to defend deception in financial markets is weak and misleading.The article elaborates on the negative impact created by spoofing and quote stuffing,the two typical deceptive practices used by high-frequency traders.The recent regulations regarding high-frequency trading,in response to the“Flash Crash”of 2010,are preventive,computerized and more effective.They reflect ethical requirements to maintain fair and stable financial markets.
文摘The Devonian Donghe Sandstone complex in North Uplift of the Tarim Basin comprises of a series of diachronous sandy intervals deposited from the Late Devonian to Early Mississippian. They are constrained by a Late Devonian to Early Pennsylvanian 2 nd-order supersequence and can be subdivided into five 3 rd-order sequences, namely, S1, S2, S3, S4, and S5, from the oldest to youngest. Cores from four wells, 40 wireline logs, 410 thin sections, and porosity and permeability data from 639 spots from four wells were used to study the sediment provenance, build up the sequence-stratigraphic model of S5, and characterize the reservoirs at a feet scale. Detrital modes of sandstone from point counting indicate that Donghe Sandstone is directly sourced from recycled orogeny. The low content of feldspar and volcanic rock fragments suggests that Donghe Sandstone is recycled from sediment with a cratonic ultimate source. 1 D and 2 D chronostratigraphic correlation shows that at least 12 4 th-order highfrequency sequences（HFSs）, from the oldest HFS1 to the youngest HFS12, can be recognized in S5. Each HFS is characterized by a general trend of shallowing-upward facies assemblage. Sequence boundaries were defined at where regionally correlatable deep-water facies overlaying shallow-water facies. There is a general shallowing-upward trend in the S5 3 rd-order sequence, characterized by a systematically increasing proportion of shallow-water facies（foreshore and upper shoreface）, and a decreasing proportion of deep facies（offshore transition and lower shoreface）. The shallowing-upward trend within both 3 rd-and 4 th-order sequences is resulted from a combined effect of eustatic sea-level change, tectonic activity, and sediment supply. The sequence-stratigraphic model of Donghe Sandstone S5 is similar to the rift-basin sequence-stratigraphic model. Sweet spots were defined as porosity 〉15% and permeability 〉100 md intervals, and their distribution and lateral continuity were investigated. HFS is one of the primary controls on the distribution of sweet spots distribution and can be used to guide hydrocarbon exploration.
文摘Wavelet transformation is a widely used method in high-frequency sequence stratigraphic analysis.However, the application is problematic since different wavelets always return the same sequence analysis results. To address this issue, we applied five commonly used wavelets to theoretical sequence models to document some application criteria. Five gradual scale-change sequence models were simplified from the glutenite succession deposition by gravity flows to form the fining-upwards cycle sequences(FUCS) and coarsening-upwards cycle sequences(CUCS). After conducting theoretical sequence model tests, the optimal wavelet(sym4) was selected and successfully used with actual data to identify the sequence boundaries. We also proposed a new method to optimize the scale of continuous wavelet transformation(CWT) for sequence boundary determination. We found that the balloon-like marks in scalograms of db4, sym4, and coif4 wavelet determine, respectively, the fourth-order sequence boundary, the thick succession sequence boundaries in FUCS, and the thick succession sequence in FUCS and CUCS. Comparing the sequence identification results shows that the asymmetric wavelets had an advantage in high-frequency sequence boundary determination and sedimentary cycle discrimination through the amplitude trend of the coefficient, in which the sym4 wavelet is the most effective. In conclusion, the asymmetry of wavelets is the first selection principle, of which asymmetric wavelets are more sensitive to sediment deposition by flood flows. The match of the wavelet between the sequence is the second selection principle, in which the correlation of time-frequency impacts the accuracy of sequence surface localization. However, the waveform of the wavelet is a visual and abstract parameter for sequence boundary detection. The appropriate wavelet for lacustrine sequence analysis is the asymmetric wavelet with a weak number of side lobes. The depositional flows, depositional process,and autogenic are three sedimentary factors that influence the sequence analysis results.
基金supported by the National Natural Science Foundation of China(Grant Nos.72032006 and 92146005).
文摘Salience theory has been proposed as a new stock trading strategy.To assess the validity of this proposal,a complex decision trading system was constructed based on salience theory,a variational mode decomposition(VMD)model,a bidirectional gated recurrent unit(BiGRU)model,and high-frequency trading.The system selected 30 Chinese new energy concept stocks,ranked the stocks using salience theory,and selected the top and bottom three stocks for two portfolios.Twelve stages were established,following which the VMD and BiGRU models were applied to the predictions.The final predicted annualized returns for the high ST(salience theory value)group A(GA)and low ST group B(GB)were 194.06%and 165.88%,respectively.This finding validates the powerful utility of salience theory and deep learning to analyze the Chinese new energy market.Moreover,it explains the theoretical practicality issues that the short selling restriction is the essential reason,or even perhaps the only reason,that leads to the strength of salience theory.
文摘Binary sequences constructed by Legendre symbols are widely used in communication and cryptography since they have many good pseudo-random properties.In this paper,we determine the 2-adic complexity of the sum sequence of any k many Legendre sequences and show that the 2-adic complexity of the sum sequences of any k many Legendre sequences reaches the maximum by proving the case of k=2 and 3,which implies that the sum sequences can resist the attack of rational approximation algorithm.
基金the National Natural Science Foundation of China(42074134,42474152,42374150)CNPC Innovation Found(2024DQ02-0152).
文摘The oil-based mud(OBM) borehole measurement environment presents significant limitations on the application of existing electrical logging instruments in high-resistance formations. In this paper, we propose a novel logging method for detection of high-resistance formations in OBM using highfrequency electrodes. The method addresses the issue of shallow depth of investigation(DOI) in existing electrical logging instruments, while simultaneously ensuring the vertical resolution. Based on the principle of current continuity, the total impedance of the loop is obtained by equating the measurement loop to the series form of a capacitively coupled circuit. and its validity is verified in a homogeneous formation model and a radial two-layer formation model with a mud standoff. Then, the instrument operating frequency and electrode system parameters were preferentially determined by numerical simulation, and the effect of mud gap on impedance measurement was investigated. Subsequently, the DOI of the instrument was investigated utilizing the pseudo-geometric factor defined by the real part of impedance. It was determined that the detection depth of the instrument is 8.74 cm, while the effective vertical resolution was not less than 2 cm. Finally, a focused high-frequency electrode-type instrument was designed by introducing a pair of focused electrodes, which effectively enhanced the DOI of the instrument and was successfully deployed in the Oklahoma formation model. The simulation results demonstrate that the novel method can achieve a detection depth of 17.40 cm in highly-resistive formations drilling with OBM, which is approximately twice the depth of detection of the existing oil-based mud microimager instruments. Furthermore, its effective vertical resolution remains at or above 2 cm,which is comparable to the resolution of the existing OBM electrical logging instrument.
文摘Objective:To analyze the significance of high-frequency ultrasound in differentiating benign and malignant breast micronodules.Methods:Eighty-five patients with breast micronodules admitted for diagnosis between October 2022 and October 2024 were selected for high-frequency ultrasound diagnosis.The diagnostic efficacy of high-frequency ultrasound was evaluated by comparing it with the results of surgical pathology.Results:High-frequency ultrasound detected 50 benign nodules,primarily breast fibroadenomas,and 35 malignant nodules,mainly breast ductal carcinoma in situ.Based on surgical pathology results,the diagnostic accuracy of high-frequency ultrasound was 96.47%,specificity was 97.96%,and sensitivity was 94.44%.In high-frequency ultrasound diagnosis,the proportion of grade III and IV blood flow in malignant nodules was higher than that in benign nodules,while the proportion of regular shape and clear margins was lower.The proportion of microcalcifications and posterior echo attenuation was higher in malignant nodules,and the resistance index(RI)and peak blood flow velocity were lower than those in benign nodules(P<0.05).Conclusion:High-frequency ultrasound can effectively differentiate benign and malignant breast micronodules,determine specific nodule types,and exhibits high diagnostic accuracy and sensitivity.Additionally,benign and malignant nodules can be differentiated based on the grading of blood flow signals,sonographic features,and blood flow velocity,providing reasonable guidance for subsequent treatment plans.
文摘China has a long history of coal mining,among which open-pit coal mines have a large number of small coal mine goafs underground.The distribution,shape,structure and other characteristics of goafs are isolated and discontinuous,and there is no definite geological law to follow,which seriously threatens the safety of coal mine production and personnel life.Conventional ground geophysical methods have low accuracy in detecting goaf areas affected by mechanical interference from open-pit mines,especially for waterless goaf areas,which cannot be detected by existing methods.This article proposes the use of high-frequency electromagnetic waves for goaf detection.The feasibility of using drilling radar to detect goaf was theoretically analyzed,and a goaf detection model was established.The response characteristics of different fillers in the goaf under different frequencies of high-frequency electromagnetic waves were simulated and analyzed.In a certain open-pit mine in Inner Mongolia,100MHz high-frequency electromagnetic waves were used to detect the goaf through directional drilling on the ground.After detection,excavation verification was carried out,and the location of one goaf detected was verified.The results of engineering practice show that the application of high-frequency electromagnetic waves in goaf detection expands the detection radius of boreholes,has the advantages of high efficiency and accuracy,and has important theoretical and practical significance.
基金supported by the science and technology project of State Grid Shanghai Municipal Electric Power Company(No.52094023003L).
文摘New electric power systems characterized by a high proportion of renewable energy and power electronics equipment face significant challenges due to high-frequency(HF)electromagnetic interference from the high-speed switching of power converters.To address this situation,this paper offers an in-depth review of HF interference problems and challenges originating from power electronic devices.First,the root cause of HF electromagnetic interference,i.e.,the resonant response of the parasitic parameters of the system to high-speed switching transients,is analyzed,and various scenarios of HF interference in power systems are highlighted.Next,the types of HF interference are summarized,with a focus on common-mode interference in grounding systems.This paper thoroughly reviews and compares various suppression methods for conducted HF interference.Finally,the challenges involved and suggestions for addressing emerging HF interference problems from the perspective of both power electronics equipment and power systems are discussed.This review aims to offer a structured understanding of HF interference problems and their suppression techniques for researchers and practitioners.
基金supported by the National Science Foundation of China(No.12127806,No.62175195 and No.12304382)the International Joint Research Laboratory for Micro/Nano Manufacturing and Measurement Technologies.
文摘Single-shot ultrafast compressed imaging(UCI)is an effective tool for studying ultrafast dynamics in physics,chemistry,or material science because of its excellent high frame rate and large frame number.However,the random code(Rcode)used in traditional UCI will lead to low-frequency noise covering high-frequency information due to its uneven sampling interval,which is a great challenge in the fidelity of large-frame reconstruction.Here,a high-frequency enhanced compressed active photography(H-CAP)is proposed.By uniformizing the sampling interval of R-code,H-CAP capture the ultrafast process with a random uniform sampling mode.This sampling mode makes the high-frequency sampling energy dominant,which greatly suppresses the low-frequency noise blurring caused by R-code and achieves high-frequency information of image enhanced.The superior dynamic performance and large-frame reconstruction ability of H-CAP are verified by imaging optical self-focusing effect and static object,respectively.We applied H-CAP to the spatial-temporal characterization of double-pulse induced silicon surface ablation dynamics,which is performed within 220 frames in a single-shot of 300 ps.H-CAP provides a high-fidelity imaging method for observing ultrafast unrepeatable dynamic processes with large frames.
文摘Objective:To analyze the therapeutic effect of high-frequency electrosurgical knife surgery guided by painless digestive endoscopy(PDE)in elderly patients with gastrointestinal polyps(GP).Methods:A total of 100 elderly GP patients admitted between June 2021 and December 2022 were selected.Patients were randomly divided into two groups:the painless group(50 cases)underwent high-frequency electrosurgical knife surgery guided by PDE,while the conventional group(50 cases)underwent the same surgery guided by traditional digestive endoscopy(DE).The total treatment efficacy,perioperative indicators,gastrointestinal hormone levels,oxidative stress(OS)markers,and complication rates were compared between the two groups.Results:The total treatment efficacy in the painless group was higher than that in the conventional group,and perioperative indicators were superior in the painless group(P<0.05).One week after treatment,the gastrointestinal hormone levels and OS-related markers in the painless group were better than those in the conventional group(P<0.05).The complication rate in the painless group was lower than in the conventional group(P<0.05).Conclusion:High-frequency electrosurgical knife surgery guided by PDE improves the effectiveness of polyp removal in elderly GP patients and accelerates postoperative recovery.It also protects gastrointestinal function,reduces postoperative OS,and ensures higher surgical safety.
基金supported by the National Key R&D Program of China(Grant No.2021YFB3501300)the 9th Research Institute of China Electronics Technology Group Corporation’s open projects(Grant No.2024SK-002-01)the Science and Technology Project of Gansu Province(Grant No.22YF7GA001).
文摘Soft magnetic composites made from metallic magnetic particles with an easy magnetization plane(referred to as easy-plane metallic soft magnetic composites(SMC))are considered ideal materials for the next generation of power electronic devices.This advantage is attributed to their ability to maintain high permeability at elevated frequencies.Despite these advantages,a definitive mathematical model that connects the high-frequency magnetic properties(e.g.,effective permeability)of easy-plane metallic SMCs to the intrinsic properties of the particles is still lacking.In this work,a theoretical calculation model for the effective permeability of easy-plane metallic SMCs was formulated.This model was derived from a skin effect-corrected Landau-Lifshitz-Gilbert(LLG)equation and integrated with effective medium theory incorporating inter-particle interaction.To validate the model,we prepared samples of easy-plane Y_(2)Co_(17)particle/PU SMCs with varying particle sizes and volume fractions.The experimental results showed a strong agreement with the calculated values.This research offers critical theoretical backing for the design and optimization of soft magnetic materials intended for high-frequency applications.
基金supported by the Commercial Aircraft Corporation of China Ltd.(Grant No.COMAC-SFGS-2024–569)Fundamental Research Funds for the Central Universities and Institute of Marine Equipment,Shanghai Rising-Star Program of Science and Technology Commission of Shanghai Municipality(Grant No.23QA1404700)+1 种基金National Natural Science Foundation of China(Grant No.52475384,52505409)China Postdoctoral Science Foundation(Grant No.2024M761963)。
文摘High-frequency pulsed(HFP)gas tungsten arc welding(GTAW)has shown excellent performance in welding of aluminum alloys in recent years,which makes itself a promisingly potential technique for part manufacturing in aviation industry.However,existing researches generally focuses on the effect of a single parameter while lacks multivariable researches.Considering of the fact that gap and misalignment are inevitable in real part clamping,adaptive intelligent welding is usually used during automatic manufacturing,which means under the control of filler wire amount per length of a weld,other parameters including current,welding speed and wire feed speed during one single weld are changing according to the specific clamping situation.Therefore,the influence of specific energy input led by different welding parameters within one adaptive welding program on microstructure and mechanical property of the weld needs to be clarified.This study investigates the effect of welding heat input(ranging from 1048.3 J/mm to 825.6 J/mm within one adaptive welding program control)on the formation quality of 3.25 mm thick 6061 aluminum alloy joints fabricated by HFP-GTAW with 4043 filler wire.According to the obtained results,non-monotonic relationship between heat input and porosity,with an optimal minimum of 4.92%achieved at an intermediate heat input of 856.8 J/mm.The 21.2%decrease of energy input during welding process would reduce the average grain size in the weld center and adjacent to fusion line by 18.6%and 19.4%,respectively.The ratios between fluctuation range to minimum value in average yield and the relative ranges of yield strength and ultimate tensile strength across the tested heat inputs were 14.7%and 12.7%,respectively.The findings provide a general overview on how the microstructure and mechanical properties would fluctuate in an adaptively controlled HFP-GTAW fabricated aluminum alloy weld.
基金National Natural Science Foundation of China, grant number 52177074.
文摘With the evolution of DC distribution networks from traditional radial topologies to more complex multi-branch structures,the number of measurement points supporting synchronous communication remains relatively limited.This poses challenges for conventional fault distance estimation methods,which are often tailored to simple topologies and are thus difficult to apply to large-scale,multi-node DC networks.To address this,a fault distance estimation method based on sparse measurement of high-frequency electrical quantities is proposed in this paper.First,a preliminary fault line identification model based on compressed sensing is constructed to effectively narrow the fault search range and improve localization efficiency.Then,leveraging the high-frequency impedance characteristics and the voltage-current relationship of electrical quantities,a fault distance estimation approach based on high-frequency measurements from both ends of a line is designed.This enables accurate distance estimation even when the measurement devices are not directly placed at both ends of the faulted line,overcoming the dependence on specific sensor placement inherent in traditional methods.Finally,to further enhance accuracy,an optimization model based on minimizing the high-frequency voltage error at the fault point is introduced to reduce estimation error.Simulation results demonstrate that the proposed method achieves a fault distance estimation error of less than 1%under normal conditions,and maintains good performance even under adverse scenarios.
基金supported by Qingdao Key Medical and Health Discipline ProjectThe Intramural Research Program of the Affiliated Hospital of Qingdao University,No. 4910Qingdao West Coast New Area Science and Technology Project,No. 2020-55 (all to SW)。
文摘Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macrophages have been poorly understood and largely overlooked. However, a recent study reported that border-associated macrophages participate in stroke-induced inflammation, although many details and the underlying mechanisms remain unclear. In this study, we performed a comprehensive single-cell analysis of mouse border-associated macrophages using sequencing data obtained from the Gene Expression Omnibus(GEO) database(GSE174574 and GSE225948). Differentially expressed genes were identified, and enrichment analysis was performed to identify the transcription profile of border-associated macrophages. CellChat analysis was conducted to determine the cell communication network of border-associated macrophages. Transcription factors were predicted using the ‘pySCENIC' tool. We found that, in response to hypoxia, borderassociated macrophages underwent dynamic transcriptional changes and participated in the regulation of inflammatory-related pathways. Notably, the tumor necrosis factor pathway was activated by border-associated macrophages following ischemic stroke. The pySCENIC analysis indicated that the activity of signal transducer and activator of transcription 3(Stat3) was obviously upregulated in stroke, suggesting that Stat3 inhibition may be a promising strategy for treating border-associated macrophages-induced neuroinflammation. Finally, we constructed an animal model to investigate the effects of border-associated macrophages depletion following a stroke. Treatment with liposomes containing clodronate significantly reduced infarct volume in the animals and improved neurological scores compared with untreated animals. Taken together, our results demonstrate comprehensive changes in border-associated macrophages following a stroke, providing a theoretical basis for targeting border-associated macrophages-induced neuroinflammation in stroke treatment.
基金Supported by the National Natural Science Foundation of China(Grant No.12161013)the School-Level Student Research Project of Guizhou University of Finance and Economics(Grant No.2024ZXSY239).
文摘In this paper,we introduce non-abelian cohomology groups and classify the nonabelian extensions of Rota-Baxter pre-Lie algebras in terms of non-abelian cohomology groups.Next,we explore the inducibility of pairs of automorphisms and derive the analog Wells exact sequences under the circumstance of Rota-Baxter pre-Lie algebras.Finally,we discuss the inducibility problem of pairs of automorphisms about an abelian extensions of Rota-Baxter pre-Lie algebras.
基金supported by the Project of the National Key Research and Development Program of China under Grant 2022YFB2404100。
文摘The internal hotspot temperature rise prediction in nanocrystalline high-frequency transformers(nanoHFTs) is essential to ensure reliable operation. This paper presents a three-dimensional thermal network(3DTN) model for epoxy resin encapsulated nano HFTs, which aims to precisely predict the temperature distribution inside the transformer in combination with the finite element method(FEM). A magnetothermal bidirectional coupling 3DTN model is established by analyzing the thermal conduction between the core, windings, and epoxy resin, while also considering the convection and radiation heat transfer mechanisms on the surface of the epoxy resin. The model considers the impact of loss distribution in the core and windings on the temperature field and adopts a simplified 1/2 thermal network model to reduce computational complexity. Furthermore, the results of FEM are compared with experimental results to verify the accuracy of the 3DTN model in predicting the temperature rise of nano HFT. The results show that the 3DTN model reduces errors by an average of 5.25% over the traditional two-dimensional thermal network(2DTN) model, particularly for temperature distributions in the windings and core. This paper provides a temperature rise prediction method for the thermal design and offers a theoretical basis and engineering guidance for the optimization of their thermal management systems.
基金supported by the National Science Foundation of China,Nos.82325031(to FX),82030059(to YC),82102290(to YG),U23A20485(to YC)Noncommunicable Chronic Diseases-National Science and Technology Major Project,No.2023ZD0505504(to FX),2023ZD0505500(to YC)the Key R&D Program of Shandong Province,No.2022ZLGX03(to YC).
文摘Global brain ischemia and neurological deficit are consequences of cardiac arrest that lead to high mortality.Despite advancements in resuscitation science,our limited understanding of the cellular and molecular mechanisms underlying post-cardiac arrest brain injury have hindered the development of effective neuroprotective strategies.Previous studies primarily focused on neuronal death,potentially overlooking the contributions of non-neuronal cells and intercellular communication to the pathophysiology of cardiac arrest-induced brain injury.To address these gaps,we hypothesized that single-cell transcriptomic analysis could uncover previously unidentified cellular subpopulations,altered cell communication networks,and novel molecular mechanisms involved in post-cardiac arrest brain injury.In this study,we performed a single-cell transcriptomic analysis of the hippocampus from pigs with ventricular fibrillation-induced cardiac arrest at 6 and 24 hours following the return of spontaneous circulation,and from sham control pigs.Sequencing results revealed changes in the proportions of different cell types,suggesting post-arrest disruption in the blood-brain barrier and infiltration of neutrophils.These results were validated through western blotting,quantitative reverse transcription-polymerase chain reaction,and immunofluorescence staining.We also identified and validated a unique subcluster of activated microglia with high expression of S100A8,which increased over time following cardiac arrest.This subcluster simultaneously exhibited significant M1/M2 polarization and expressed key functional genes related to chemokines and interleukins.Additionally,we revealed the post-cardiac arrest dysfunction of oligodendrocytes and the differentiation of oligodendrocyte precursor cells into oligodendrocytes.Cell communication analysis identified enhanced post-cardiac arrest communication between neutrophils and microglia that was mediated by neutrophil-derived resistin,driving pro-inflammatory microglial polarization.Our findings provide a comprehensive single-cell map of the post-cardiac arrest hippocampus,offering potential novel targets for neuroprotection and repair following cardiac arrest.
基金supported by the National Science Foundation of China(No.62171387)the Science and Technology Program of Sichuan Province(No.2024NSFSC0468)the China Postdoctoral Science Foundation(No.2019M663475).
文摘As an important resource in data link,time slots should be strategically allocated to enhance transmission efficiency and resist eavesdropping,especially considering the tremendous increase in the number of nodes and diverse communication needs.It is crucial to design control sequences with robust randomness and conflict-freeness to properly address differentiated access control in data link.In this paper,we propose a hierarchical access control scheme based on control sequences to achieve high utilization of time slots and differentiated access control.A theoretical bound of the hierarchical control sequence set is derived to characterize the constraints on the parameters of the sequence set.Moreover,two classes of optimal hierarchical control sequence sets satisfying the theoretical bound are constructed,both of which enable the scheme to achieve maximum utilization of time slots.Compared with the fixed time slot allocation scheme,our scheme reduces the symbol error rate by up to 9%,which indicates a significant improvement in anti-interference and eavesdropping capabilities.
