Objective: To predict the outcome of dysplasia of esophageal epithelium by means of high resolution image analysis (HRIA). Methods: Asymptomatic adults were examined for balloon cytology of the esophagus in 1983 from ...Objective: To predict the outcome of dysplasia of esophageal epithelium by means of high resolution image analysis (HRIA). Methods: Asymptomatic adults were examined for balloon cytology of the esophagus in 1983 from Heshun Commune of Linxian County. 93 severe dysplasias and 122 mild dysplasias of the esophagus were selected. By means of an Axiomat-microscope equipped with TV-camera, 100 normal nuclei of well-preserved cells in the intermediate layer of Pap-stained squamous epithelium were randomly examined. Results: Of the 93 cytologically diagnosed severe dysplasia cases, 24, 14 and 7 progressed to carcinoma in 3, 5 and 9 years, respectively. In the other 48 cases, dysplasia remained stable or regressed to normal. The other cases were used as the control. According to chromatin features, correct diagnosis of cases was achieved by HRIA in 75.0% (18/24), 85.7% (12/14) and 85.7% (6/7) of the cases examined, respectively (P<0.001). Of the 122 cytologically diagnosed mild dysplasia, 16, 13 and 12 cases progressed to carcinoma in 3, 5 and 9 years, respectively. The other 81 cases remained stable or regressed to normal. Correct diagnosis was made by HRIA in 93.8% (15/16), 76.9% (10/13) and 83.3% (10/12) of the cases examined, respective1y (P<0.001). Conclusion: Chromatin nuclear features examined by HRIA can predict the outcome of precancerous lesions and discriminate progressor from non-progressor ones. It can be used as surrogate endpoint biomarkers for the evaluation of efficiency of chemo-prevention trial.展开更多
Background: Pyrazinamide (PZA) is one of the most important drugs for tuberculosis (TB) treatment, however, its susceptibility is not routinely tested. High-resolution melting (HRM) curve analysis has been widely used...Background: Pyrazinamide (PZA) is one of the most important drugs for tuberculosis (TB) treatment, however, its susceptibility is not routinely tested. High-resolution melting (HRM) curve analysis has been widely used for many applications. In this study, HRM assay was developed and evaluated for the detection of PZA resistance in Mycobacterium tuberculosis clinical isolates. Methods: Ninety five M. tuberculosis clinical isolates with different susceptibility patterns to anti-TB drugs were used to evaluate this assay. Isolates were phenotypically (Bactec MGIT 960) and genotypically (HRM and pncA gene sequencing) analysed for PZA resistance. Results: Bactec MGIT 960 analysis revealed that 29 of the 95 M. tuberculosis isolates were PZA resistant. In comparison to the Bactec MGIT 960, HRM showed a sensitivity of 47.7% and specificity of 74.6%, and the overall agreement between the two methods was 68.4%. Based on DNA sequencing, a correlation of 0.67 (significant at p-value pncA mutations was observed. PZA resistance was strongly associated with multi-drug resistant (MDR)-TB as it was shown in 79.3% of the MDR isolates included in the study. Conclusion: HRM is simple and useful for screening clinical M. tuberculosis isolates for PZA resistance, however, further modifications to improve its performance are required.展开更多
The analysis of single cells instead of cell populations is important for characterizing cellular heterogeneity and elucidating the cellular signalling pathways. Nanoelectrodes have emerged as an increasingly importan...The analysis of single cells instead of cell populations is important for characterizing cellular heterogeneity and elucidating the cellular signalling pathways. Nanoelectrodes have emerged as an increasingly important tool for biomolecule analyses at the single-cell level with high spatial or temporal resolution. Various electrochemical methods, such as amperometry and scanning electrochemical microscopy(SECM), have been applied. Research to date has focused on the development of new nanoelectrochemical architectures, such as arrays, to achieve higher spatial resolution and faster analysis rates for single-cell analysis. In this review, the fabrication of these new nanoelectrochemical architectures and their applications in high spatial resolution single-cell analyses are discussed. The recent progress of Chinese researchers is highlighted.展开更多
LA-MC-ICP-MS offers the ability to directly measure small variations in isotope composition at the micrometer scale in geological samples.However,when analyzing isotope ratios in complex minerals,mass spectrometric in...LA-MC-ICP-MS offers the ability to directly measure small variations in isotope composition at the micrometer scale in geological samples.However,when analyzing isotope ratios in complex minerals,mass spectrometric interferences from themselves and isotopic mass fractionation arising from the analytical process need to be carefully corrected.The lack of professional software to visualize and process raw data presents a significant challenge for all of LA-MC-ICP-MS labs.Moreover,the rapid development of instruments and the innovations in micro-analytical techniques necessitate specialized software to complete the complex correction and improve efficiency,such as for in situ isochron dating or new linear regression correction techniques.In this study,Plume,a free data processing software,was developed specifically for isotopic data processing in LA-MC-ICP-MS.The software provides a comprehensive set of functions,including baseline correction,signal selection and integration,mass spectrometric interference correction,isotopic fractionation correction,uncertainty propagation,delta value calculation,and real-time data processing.It also supports a complete correction workflow for the latest isochron dating using LA-(MC)-ICP-MS/MS,including isotopic and elemental ratios correction,the isochron drawing,and age calculation for multiple models.Additionally,a dedicated linear regression calculation unit has been developed in Plume to specialize in data processing of low signal-to-noise ratio or high spatial resolution analysis.These new features expand the application range of LA-MC-ICP-MS and improve work efficiency through batch processing.展开更多
基金a grant from the National Eighth Five-year Plan Foundation of China !(No. 85-914-01-02).
文摘Objective: To predict the outcome of dysplasia of esophageal epithelium by means of high resolution image analysis (HRIA). Methods: Asymptomatic adults were examined for balloon cytology of the esophagus in 1983 from Heshun Commune of Linxian County. 93 severe dysplasias and 122 mild dysplasias of the esophagus were selected. By means of an Axiomat-microscope equipped with TV-camera, 100 normal nuclei of well-preserved cells in the intermediate layer of Pap-stained squamous epithelium were randomly examined. Results: Of the 93 cytologically diagnosed severe dysplasia cases, 24, 14 and 7 progressed to carcinoma in 3, 5 and 9 years, respectively. In the other 48 cases, dysplasia remained stable or regressed to normal. The other cases were used as the control. According to chromatin features, correct diagnosis of cases was achieved by HRIA in 75.0% (18/24), 85.7% (12/14) and 85.7% (6/7) of the cases examined, respectively (P<0.001). Of the 122 cytologically diagnosed mild dysplasia, 16, 13 and 12 cases progressed to carcinoma in 3, 5 and 9 years, respectively. The other 81 cases remained stable or regressed to normal. Correct diagnosis was made by HRIA in 93.8% (15/16), 76.9% (10/13) and 83.3% (10/12) of the cases examined, respective1y (P<0.001). Conclusion: Chromatin nuclear features examined by HRIA can predict the outcome of precancerous lesions and discriminate progressor from non-progressor ones. It can be used as surrogate endpoint biomarkers for the evaluation of efficiency of chemo-prevention trial.
文摘Background: Pyrazinamide (PZA) is one of the most important drugs for tuberculosis (TB) treatment, however, its susceptibility is not routinely tested. High-resolution melting (HRM) curve analysis has been widely used for many applications. In this study, HRM assay was developed and evaluated for the detection of PZA resistance in Mycobacterium tuberculosis clinical isolates. Methods: Ninety five M. tuberculosis clinical isolates with different susceptibility patterns to anti-TB drugs were used to evaluate this assay. Isolates were phenotypically (Bactec MGIT 960) and genotypically (HRM and pncA gene sequencing) analysed for PZA resistance. Results: Bactec MGIT 960 analysis revealed that 29 of the 95 M. tuberculosis isolates were PZA resistant. In comparison to the Bactec MGIT 960, HRM showed a sensitivity of 47.7% and specificity of 74.6%, and the overall agreement between the two methods was 68.4%. Based on DNA sequencing, a correlation of 0.67 (significant at p-value pncA mutations was observed. PZA resistance was strongly associated with multi-drug resistant (MDR)-TB as it was shown in 79.3% of the MDR isolates included in the study. Conclusion: HRM is simple and useful for screening clinical M. tuberculosis isolates for PZA resistance, however, further modifications to improve its performance are required.
基金supported by the National Natural Science Foundation of China (21327902)
文摘The analysis of single cells instead of cell populations is important for characterizing cellular heterogeneity and elucidating the cellular signalling pathways. Nanoelectrodes have emerged as an increasingly important tool for biomolecule analyses at the single-cell level with high spatial or temporal resolution. Various electrochemical methods, such as amperometry and scanning electrochemical microscopy(SECM), have been applied. Research to date has focused on the development of new nanoelectrochemical architectures, such as arrays, to achieve higher spatial resolution and faster analysis rates for single-cell analysis. In this review, the fabrication of these new nanoelectrochemical architectures and their applications in high spatial resolution single-cell analyses are discussed. The recent progress of Chinese researchers is highlighted.
基金supported by the National Key Research and Development Program of China(Grant No.2024YFF0808200)the National Natural Science Foundation of China(Grant Nos.42330104,42473035)+1 种基金Hubei Provincial Natural Science Foundation of China(Grant No.2025AFA005)the Most Special Fund from the State Key Laboratory of Geological Processes and Mineral Resources,China University of Geosciences(Grant Nos.MSFGPMR04 and MSFGPMR08)。
文摘LA-MC-ICP-MS offers the ability to directly measure small variations in isotope composition at the micrometer scale in geological samples.However,when analyzing isotope ratios in complex minerals,mass spectrometric interferences from themselves and isotopic mass fractionation arising from the analytical process need to be carefully corrected.The lack of professional software to visualize and process raw data presents a significant challenge for all of LA-MC-ICP-MS labs.Moreover,the rapid development of instruments and the innovations in micro-analytical techniques necessitate specialized software to complete the complex correction and improve efficiency,such as for in situ isochron dating or new linear regression correction techniques.In this study,Plume,a free data processing software,was developed specifically for isotopic data processing in LA-MC-ICP-MS.The software provides a comprehensive set of functions,including baseline correction,signal selection and integration,mass spectrometric interference correction,isotopic fractionation correction,uncertainty propagation,delta value calculation,and real-time data processing.It also supports a complete correction workflow for the latest isochron dating using LA-(MC)-ICP-MS/MS,including isotopic and elemental ratios correction,the isochron drawing,and age calculation for multiple models.Additionally,a dedicated linear regression calculation unit has been developed in Plume to specialize in data processing of low signal-to-noise ratio or high spatial resolution analysis.These new features expand the application range of LA-MC-ICP-MS and improve work efficiency through batch processing.
