The fruit of Manilkara hexandra(Roxb.)Dubard is one of the most underutilized fruits of India and in Gujarat state.It is popularly known as‘Rayan’.The fruit and seed of Rayan were analysed for their total phenolic a...The fruit of Manilkara hexandra(Roxb.)Dubard is one of the most underutilized fruits of India and in Gujarat state.It is popularly known as‘Rayan’.The fruit and seed of Rayan were analysed for their total phenolic and flavonoid content,phenolic compounds and total antioxidant capacity with six different assay methods.The results indicated that the methanolic extract of Rayan fruit being a good source of phenolic(811.3 mg GAE/100 g fw)and flavonoid(485.56 mg RE/100 g fw)content.Also,eleven known phenolic compounds were tentatively identified for the first time from the fruit and seed of Rayan.The LC–MS/MS analysis of fruit revealed the presence of major phenolic compounds such as gallic acid,quercetin and kaempferol,while quercetin,gallic acid and vanillic acid in seed.The presence of quercetin suggests health benefits.The fruit of Rayan was also proved to be a better source of antioxidants as measured by FRAP,RPA,DPPHRSA,ABTSRSA and HRSA except NORSA in comparison with that of seed.The current study explains that M.hexandra is a relatively good source of antioxidants such as phenols and flavonoids for diet.展开更多
Randomly amplified polymorphic DNA (RAPD) technique was applied to assess the genetic variations and phylogenetic relationships in genetic differentiation within 4 Chromium-treatment Leersia hexandra. The fresh leaves...Randomly amplified polymorphic DNA (RAPD) technique was applied to assess the genetic variations and phylogenetic relationships in genetic differentiation within 4 Chromium-treatment Leersia hexandra. The fresh leaves of Leersia hexandra cultivated on the condition of chrome pollution and exogenous organic acids were used as experimental material. The genomic DNA of Leersia hexandra was extracted by using CTAB method. The results showed that different samples of Leersia hexandra exhibited DNA polymorphism when using the random primer S43, S51and S55 as the primers in the RAPD reaction. One specific DNA band about 1000 bp was found in the sample which treated with 10 mmol/L concentration EDTA when used the S43 primer to RAPD. The obvious differences between different EDTA-treatment levels suggest that EDTA has certain effects on enrichment to heavy metals of Leersia hexandra, it will be more favored to Leersia hexandra accumulation of chromium when EDTA concentration increased.展开更多
Phytoextraction is a promising option for purifying hexavalent chromium(Cr(Ⅵ))-laden wastewater,but the long remediation period incurred by poor growth rate of Cr hyperaccumulators remains a primary hindrance to its ...Phytoextraction is a promising option for purifying hexavalent chromium(Cr(Ⅵ))-laden wastewater,but the long remediation period incurred by poor growth rate of Cr hyperaccumulators remains a primary hindrance to its large-scale application.In this study,we performed a hydroponic experiment to evaluate the feasibility of promoting the growth and phytoextraction efficiency of Cr hyperaccumulator Leersia hexandra Swartz(L.hexandra)by inoculating plant growth-promoting rhizobacteria(PGPR)Bacillus cereus(B.cereus).In batch tests,the Cr(Ⅵ)removal rates of L.hexandra and B.cereus co-culture were greater than the sum of their respective monocultures.This was likely due to the microbial reduction of Cr(Ⅵ)to Cr(Ⅲ),which is amiable to plant uptake.Besides,the PGPR factors of B.cereus,including indoleacetic acid(IAA)production,1-aminocyclopropane-1-carboxylic acid deamination(ACCd)activity,phosphate solubilization capacity,and siderophore production,were quantified.These PGPR factors helped explain the biomass augmentation,root elongation and enhanced Cr enrichment of the inoculated L.hexandra in pot experiments.Despite the increased Cr uptake,no aggravated oxidative damage to the cell membrane was observed in the inoculated L.hexandra.This was attributed to its capacity to confront the increased intracellular Cr stress by upregulating both the activities of antioxidative enzymes and expression of metal-binding proteins/peptides.Moreover,L.hexandra could always conserve the majority of Cr in the residual and oxalic integrated forms with low mobility and phytotoxicity,irrespective of the B.cereus inoculation.These results highlight the constructed Cr hyperaccumulatorrhizobacteria consortia as an effective candidate for decontaminating Cr(Ⅵ)-laden wastewater.展开更多
Background:Manilkara hexandra(Roxb.)dubard is an evergreen tree species that belongs to the Sapotaceae family which has been used in traditional system of medicines since ages in South Asia,especially in Western and C...Background:Manilkara hexandra(Roxb.)dubard is an evergreen tree species that belongs to the Sapotaceae family which has been used in traditional system of medicines since ages in South Asia,especially in Western and Central India.Objective:This review aims to include a thorough report on phytochemicals found in the various sections of the plant,which are responsible for their medicinal properties in turn.Method:All the informations in this article were collated from selected research papers from online database such as PubMed,Web of Science and Scopus.Results:An audit of its literature shows extracts from this plant and bio transformed products possess different activities such as antibacterial,anti-inflammatory,antiulcer,aphrodisiac,immuno-stimulation activity,arthritic activity,and free radical scavenging activity.Apart from therapeutic indications,the products obtained such as edible and nutritious fruit,valuable wood,latex,and bark,are cost effective and are a source of living for local people.The varieties of chemical compounds have been isolated from this species,including sterols,sugar,terpenoids,anthraquinone glycoside,cardiac glycoside,saponin and tannins,etc.Worldwide the large occurrence of this underutilized species offers an incentive to use this plant at crucial levels in the treatment of various ailments.Conclusion:This review attempts to systematically collate the scattered knowledge on M.hexandra,for its botan-ical elements,phytochemistry,ethnopharmacology,and biological activities,to assess the range of variation among natural populations in opening research areas,and to identify elite cultivars for popularization among farmers/tribal for conservation and sustainable use on conducting field improvement studies.展开更多
文摘The fruit of Manilkara hexandra(Roxb.)Dubard is one of the most underutilized fruits of India and in Gujarat state.It is popularly known as‘Rayan’.The fruit and seed of Rayan were analysed for their total phenolic and flavonoid content,phenolic compounds and total antioxidant capacity with six different assay methods.The results indicated that the methanolic extract of Rayan fruit being a good source of phenolic(811.3 mg GAE/100 g fw)and flavonoid(485.56 mg RE/100 g fw)content.Also,eleven known phenolic compounds were tentatively identified for the first time from the fruit and seed of Rayan.The LC–MS/MS analysis of fruit revealed the presence of major phenolic compounds such as gallic acid,quercetin and kaempferol,while quercetin,gallic acid and vanillic acid in seed.The presence of quercetin suggests health benefits.The fruit of Rayan was also proved to be a better source of antioxidants as measured by FRAP,RPA,DPPHRSA,ABTSRSA and HRSA except NORSA in comparison with that of seed.The current study explains that M.hexandra is a relatively good source of antioxidants such as phenols and flavonoids for diet.
文摘Randomly amplified polymorphic DNA (RAPD) technique was applied to assess the genetic variations and phylogenetic relationships in genetic differentiation within 4 Chromium-treatment Leersia hexandra. The fresh leaves of Leersia hexandra cultivated on the condition of chrome pollution and exogenous organic acids were used as experimental material. The genomic DNA of Leersia hexandra was extracted by using CTAB method. The results showed that different samples of Leersia hexandra exhibited DNA polymorphism when using the random primer S43, S51and S55 as the primers in the RAPD reaction. One specific DNA band about 1000 bp was found in the sample which treated with 10 mmol/L concentration EDTA when used the S43 primer to RAPD. The obvious differences between different EDTA-treatment levels suggest that EDTA has certain effects on enrichment to heavy metals of Leersia hexandra, it will be more favored to Leersia hexandra accumulation of chromium when EDTA concentration increased.
基金supported by the National Natural Science Foundation of China(Nos.52000046,52100034,52170154,and 52070051)the Special Project of Guangxi Science and Technology Base and Talent(Nos.GuiKe AD20297009 and GuiKe AD20297007)the Middle-aged and Young Teachers’Basic Ability Promotion Project of Guangxi(Nos.2020KY05039 and 2021KY0221).
文摘Phytoextraction is a promising option for purifying hexavalent chromium(Cr(Ⅵ))-laden wastewater,but the long remediation period incurred by poor growth rate of Cr hyperaccumulators remains a primary hindrance to its large-scale application.In this study,we performed a hydroponic experiment to evaluate the feasibility of promoting the growth and phytoextraction efficiency of Cr hyperaccumulator Leersia hexandra Swartz(L.hexandra)by inoculating plant growth-promoting rhizobacteria(PGPR)Bacillus cereus(B.cereus).In batch tests,the Cr(Ⅵ)removal rates of L.hexandra and B.cereus co-culture were greater than the sum of their respective monocultures.This was likely due to the microbial reduction of Cr(Ⅵ)to Cr(Ⅲ),which is amiable to plant uptake.Besides,the PGPR factors of B.cereus,including indoleacetic acid(IAA)production,1-aminocyclopropane-1-carboxylic acid deamination(ACCd)activity,phosphate solubilization capacity,and siderophore production,were quantified.These PGPR factors helped explain the biomass augmentation,root elongation and enhanced Cr enrichment of the inoculated L.hexandra in pot experiments.Despite the increased Cr uptake,no aggravated oxidative damage to the cell membrane was observed in the inoculated L.hexandra.This was attributed to its capacity to confront the increased intracellular Cr stress by upregulating both the activities of antioxidative enzymes and expression of metal-binding proteins/peptides.Moreover,L.hexandra could always conserve the majority of Cr in the residual and oxalic integrated forms with low mobility and phytotoxicity,irrespective of the B.cereus inoculation.These results highlight the constructed Cr hyperaccumulatorrhizobacteria consortia as an effective candidate for decontaminating Cr(Ⅵ)-laden wastewater.
基金support from the database(literature survey)avail-able from library of Chitkara College of pharmacy and the Knowledge Centre of Chitkara University.
文摘Background:Manilkara hexandra(Roxb.)dubard is an evergreen tree species that belongs to the Sapotaceae family which has been used in traditional system of medicines since ages in South Asia,especially in Western and Central India.Objective:This review aims to include a thorough report on phytochemicals found in the various sections of the plant,which are responsible for their medicinal properties in turn.Method:All the informations in this article were collated from selected research papers from online database such as PubMed,Web of Science and Scopus.Results:An audit of its literature shows extracts from this plant and bio transformed products possess different activities such as antibacterial,anti-inflammatory,antiulcer,aphrodisiac,immuno-stimulation activity,arthritic activity,and free radical scavenging activity.Apart from therapeutic indications,the products obtained such as edible and nutritious fruit,valuable wood,latex,and bark,are cost effective and are a source of living for local people.The varieties of chemical compounds have been isolated from this species,including sterols,sugar,terpenoids,anthraquinone glycoside,cardiac glycoside,saponin and tannins,etc.Worldwide the large occurrence of this underutilized species offers an incentive to use this plant at crucial levels in the treatment of various ailments.Conclusion:This review attempts to systematically collate the scattered knowledge on M.hexandra,for its botan-ical elements,phytochemistry,ethnopharmacology,and biological activities,to assess the range of variation among natural populations in opening research areas,and to identify elite cultivars for popularization among farmers/tribal for conservation and sustainable use on conducting field improvement studies.
