This paper examines Hernán Díaz’s novel Trust (2022) through the lens of cultural deconstruction, analyzing how its four nested narratives systematically dismantle the American Dream ideology. The novel Bon...This paper examines Hernán Díaz’s novel Trust (2022) through the lens of cultural deconstruction, analyzing how its four nested narratives systematically dismantle the American Dream ideology. The novel Bonds and autobiography My Life collaboratively construct a financial myth portraying protagonist Andrew Bevel as an exemplar of self-made success. Subsequently, Ida’s Memoir exposes the subjective construction of history, declaring the American Dream’s bankruptcy at the epistemological level, while Mildred’s diary Futures deconstructs the myth from within by revealing that Bevel’s financial genius was built upon appropriation of his wife’s intellectual labor. This paper argues that Trust reveals the American Dream as an ideological illusion-narratively constructed, practically dependent on structural injustice, and essentially serving power reproduction.展开更多
Heme is a key cofactor in aerobic life, both in eukaryotes and prokaryotes. Because of the high reactivity of ferrous protoporphyrin IX, the reactions of heme in cells are often carried out through heme-protein comple...Heme is a key cofactor in aerobic life, both in eukaryotes and prokaryotes. Because of the high reactivity of ferrous protoporphyrin IX, the reactions of heme in cells are often carried out through heme-protein complexes. Traditionally studies of heme-binding proteins have been approached on a case by case basis, thus there is a limited global view of the distribution of heme-binding proteins in different cells or tissues. The procedure described here is aimed at profiling heme-binding proteins in mouse tissues sequentially by 1) purification of heme-binding proteins by heme-agarose, an affinity chromatographic resin; 2) isolation of heme-binding proteins by SDS-PAGE or two-dimensional electrophoresis; 3) identification of heme-binding proteins by mass spectrometry. In five mouse tissues, over 600 protein spots were visualized on 2DE gel stained by Commassie blue and 154 proteins were identified by MALDI-TOF, in which most proteins belong to heme related. This methodology makes it possible to globally characterize the heme-binding proteins in a biological system.展开更多
文摘This paper examines Hernán Díaz’s novel Trust (2022) through the lens of cultural deconstruction, analyzing how its four nested narratives systematically dismantle the American Dream ideology. The novel Bonds and autobiography My Life collaboratively construct a financial myth portraying protagonist Andrew Bevel as an exemplar of self-made success. Subsequently, Ida’s Memoir exposes the subjective construction of history, declaring the American Dream’s bankruptcy at the epistemological level, while Mildred’s diary Futures deconstructs the myth from within by revealing that Bevel’s financial genius was built upon appropriation of his wife’s intellectual labor. This paper argues that Trust reveals the American Dream as an ideological illusion-narratively constructed, practically dependent on structural injustice, and essentially serving power reproduction.
文摘Heme is a key cofactor in aerobic life, both in eukaryotes and prokaryotes. Because of the high reactivity of ferrous protoporphyrin IX, the reactions of heme in cells are often carried out through heme-protein complexes. Traditionally studies of heme-binding proteins have been approached on a case by case basis, thus there is a limited global view of the distribution of heme-binding proteins in different cells or tissues. The procedure described here is aimed at profiling heme-binding proteins in mouse tissues sequentially by 1) purification of heme-binding proteins by heme-agarose, an affinity chromatographic resin; 2) isolation of heme-binding proteins by SDS-PAGE or two-dimensional electrophoresis; 3) identification of heme-binding proteins by mass spectrometry. In five mouse tissues, over 600 protein spots were visualized on 2DE gel stained by Commassie blue and 154 proteins were identified by MALDI-TOF, in which most proteins belong to heme related. This methodology makes it possible to globally characterize the heme-binding proteins in a biological system.
