Phytic acid (Myo-inositol 1,2,3,4,5,6 hexa-kisphophate) is a storage form of phosphorus and can accumulate to the levels as high as 35% in the wheat kernel. Phytic acid acts as an inhibitor for macronutrients as well ...Phytic acid (Myo-inositol 1,2,3,4,5,6 hexa-kisphophate) is a storage form of phosphorus and can accumulate to the levels as high as 35% in the wheat kernel. Phytic acid acts as an inhibitor for macronutrients as well as micronutrients and located in the bran of wheat kernel. Due to its inhibitory role, a high concentration of phytic acid is undesirable as it hinders the bio-availability of some essential nutrients such as Fe, Mg, Ca, Zn and Cu, etc. In order to check the inheritance of phytic acid in wheat kernels, phytic acid concentration was initially determined in kernels of 10 wheat genotypes to identify two contrasting genetic groups for diallel analysis. Based on pre-screening results of 10 wheat genotypes, five wheat genotypes (3 with high and 2 with low phytic acid concentration) were crossed in all possible combinations during 2007-2008 by 5 × 5 full diallel mating fashion to insight the inheritance of phytic acid and other yield contributing traits. All 20 F1 hybrids and five parental genotypes revealed significant differences statistically, except plant maturity. The narrow and broad sense heritability estimates varied widely among traits for spike length (0.17, 0.62), spikelets spike-1 (0.35, 0.74), tillers plant-1 (0.05, 0.52) and phytic acid concentration (0.01, 0.86). The values for phytic acid concentration ranged from 0.56% to 3.43% among F1 hybrids and 1.06 to 3.67% for parental genotypes. F1 hybrids, Ps-2005 × Ghaznavi (0.56%), AUP-4006 × Ps-2004 (0.74%), Janbaz × Ps-2004 (0.89%) and Janbaz × Ps-2005 (1.01%), had the lowest concentration of phytic acid. The study concluded that F1 hybrids with low phytic acid concentration could yield desirable segregants.展开更多
The genetic resistance to angular leaf spot （ALS） caused by Phaeoisariopsis griseola in the common bean cultivar Mexico 54 was investigated on disease reactions in parental, F1, F2 and backcross generations derived ...The genetic resistance to angular leaf spot （ALS） caused by Phaeoisariopsis griseola in the common bean cultivar Mexico 54 was investigated on disease reactions in parental, F1, F2 and backcross generations derived from crosses between a resistant cultivar Mexico 54 and a susceptible cultivar Kablanketi under screen house conditions. The heritability （h2） estimate was as high as 0.719 indicating a successful transfer of ALS resistance among progenies and thus selection can be performed in early generation. High heritability coupled with high expected genetic advance of 39.5% is considered to be more useful in predicting the outcome of selecting the best individuals. Chi-square values were computed to determine whether the observed ratios for disease reactions deviated from expected Mendelian ratios for a single, dominant gene controlling resistance to angular leaf spot in common bean. Based on the resistance of the F2, and the backcross generation to the resistant parent, a 3 resistant： 1 susceptible segregation ratio in the F2 and a 1 resistant： 1 susceptible segregation ratio in the backcross generation to the susceptible parent was obtained implying that resistance to the isolate ofPhaeoisariopsis griseola is governed by a single, dominant gene.展开更多
Chinese culture has a long history. Native culture, as an important part of China's traditional culture heritage, is rich in variety. In characteristic towns, native culture has strong local characteristics, and i...Chinese culture has a long history. Native culture, as an important part of China's traditional culture heritage, is rich in variety. In characteristic towns, native culture has strong local characteristics, and its protection and heritage has great value. This paper aimed to inherit traditional culture, protect the local characteristics of the towns, endow the town landscape with a unique cultural value, enhance people's cultural cohesion and identity of local culture and create characteristic towns with strong cultural characteristics.展开更多
CRISPR/Cas （clustered regularly interspaced short palindromic repeats/CR1SPR-associated proteins） is an adaptive immune system in bacteria and archaea to defend against invasion from foreign DNA fragments. Recently,...CRISPR/Cas （clustered regularly interspaced short palindromic repeats/CR1SPR-associated proteins） is an adaptive immune system in bacteria and archaea to defend against invasion from foreign DNA fragments. Recently, it has been developed as a powerful targeted genome editing tool for a wide variety of species. However, its application in maize has only been tested with transiently expressed somatic cells or with a limited number of stable transgenic To plants. The exact efficiency and specificity of the CRISPR/Cas system in the highly complex maize genome has not been documented yet. Here we report an extensive study of the well-studied type II CRISPR-Cas9 system for targeted genome editing in maize, with the codon-optimized Cas9 protein and the short non-coding guide RNA generated through a functional maize U6 snRNA promoter. Targeted gene mutagenesis was detected for 90 loci by maize protoplast assay, with an average cleavage efficiency of 10.67 %. Stable knockout transformants for maize phytoene synthase gene （PSYI） were obtained. Mutations occurred in germ ceils can be stably inherited to the next generation. Moreover, no off-target effect was detected at the computationally predicted putative off-target loci. No significant difference between the transcriptomes of the Cas9 expressed and non-expressed lines was detected. Our results confirmed that the CRISPR-Cas9 could be successfully applied as a robust targeted genome editing system in maize.展开更多
Tree growth traits （tree height, DBH and stem volume） and survival from two 9-year-old, open-pollinated progeny tests of Chinese fir were investigated for heri- tability, genotype × environment interaction, age...Tree growth traits （tree height, DBH and stem volume） and survival from two 9-year-old, open-pollinated progeny tests of Chinese fir were investigated for heri- tability, genotype × environment interaction, age-age genetic correlation and selection efficiency. The 97 and 79 families planted at two sites were collected from the thirdcycle seed orchard. Individual heritability was estimated between 0.05 and 0.21 for tree height, DBH, and volume and between 0.45 and 1.0 for survival. Family heritability was between 0.20 and 1.14. Significant genotype x envi- ronment interaction was observed for the three growth traits. Type B genetic correlation was between 0.41 and 0.67 with an increasing trend as tree grows. High age-age genetic correlation was observed with correlation reaching 0.9 after age 4 for height, DBH, and volume. The genetic gains were estimated at 3.26, 3.39 and 5.98 % for tree height, DBH, and volume with 10 % selection intensity. The implication for advanced tree breeding in Chinese fir is discussed.展开更多
The growth performance of twenty poplar （Populus deltoides Bartr.） clones, planted in the central-plain region of Punjab in north-western India, was evaluated using a randomized block design with three replications ...The growth performance of twenty poplar （Populus deltoides Bartr.） clones, planted in the central-plain region of Punjab in north-western India, was evaluated using a randomized block design with three replications and plot size of four plants. Significant differences among clones were observed for diameter at breast height （DBH）, height and volume per tree at age five, six and eight years. Clone L-48/89 recorded significantly superior volume than the control （G-48） at all ages, with superiority of 51.4, 43.9 and 48.5 per cent at age five, six and eight years, respectively. The phenotypic and genotypic coefficients of variation were the highest for volume （26.55-34.66% and 15.84-26.00%） and the minimum for tree height （8.43-12.13% and 4.71-7.59%）. The broad sense heritability was relatively higher for DBH （0.42-0.55） and genetic advance as per cent of mean was highest for volume （19.48-40.18）. All genetic parameters increased with age. All traits showed significant positive correlation with each other.展开更多
Guar [Cyamopsis tetragonoloba （L.） Taub.] is one of the most important industrial crops due to its richness in gum. Understanding the inheritance of gum content is a key to its successful genetic improvement. Gum co...Guar [Cyamopsis tetragonoloba （L.） Taub.] is one of the most important industrial crops due to its richness in gum. Understanding the inheritance of gum content is a key to its successful genetic improvement. Gum content expression is reported to be controlled by additive, dominant, and epistatic effects, and modified by the environment, fg%, a relative gum content calculated by comparing with the gum content of Kinman was used as gum content （%） in this experiment. Reciprocal crosses of two lines of guar, PI 217923 and Lewis, were made to study the heritability of gum content （fg%）. fg% of four plant introductions and four commercial varieties were studied in Lubbock in 1999-2002. Estimates of broadsense heritability （h^2 b,s.） of fg% in Lewis x PI 217923 and PI 217923 x Lewis were 75.53 and 52.74%, respectively. Estimates of narrow-sense heritability （h2 n.s.） of fg% were 40.00 and 29.00% thr Lewis x PI 217923 and PI 217923 x Lewis, respectively. At least one pair of genes were estimated to control the fg% expression in these two crosses, Significant differences of fg% were found among these eight entries. PI 217923 was found to have the highest fg% among the eight entries.展开更多
We have characterized the number of ovules per ovary (NoOV) in cacao plants originated from crossing the clones CCN 51 and TSH 1188 (segregating progeny) and determined the heritability of this characteristic in order...We have characterized the number of ovules per ovary (NoOV) in cacao plants originated from crossing the clones CCN 51 and TSH 1188 (segregating progeny) and determined the heritability of this characteristic in order to select plants with higher NoOV in this progeny. The NoOV was calculated as the average of 10 flowers per plant and ranged from 44.8 to 58.6 between the six clones (two parents and four clones belonging to their genealogy). In the progeny (n = 209 plants) the NoOV averaged 54.3 (range 44.1 to 67.8). The NoOV was distributed uniformly among the progeny indicating that this trait is conditioned by polygenes. Its heritability was estimated at 67.7%. The 32 plants with NoOV similar or superior to the genitor CCN 51 (highest amount among clones) were selected for use in the genetic improvement program. We showed that this population is suitable for genetic mapping, molecular marker identification and selection of superior cacao genotypes.展开更多
From the“Six Rising Dragons”emerging at the Hangzhou Future Sci-Tech City(Haichuang Park)to the archaeological wonders of Liangzhu,widely regarded as a cradle of Chinese civilization,dating back 5000 years.Recently,...From the“Six Rising Dragons”emerging at the Hangzhou Future Sci-Tech City(Haichuang Park)to the archaeological wonders of Liangzhu,widely regarded as a cradle of Chinese civilization,dating back 5000 years.Recently,our reporter has been conducting an in-depth interview in Zhejiang.Marveling at the ingenuity of ancient civilizations,we were amazed by the cutting-edge“intelligence”of humanoid robotics,and unlocked a unique dialogue between millennia-old heritage and the technology shaping tomorrow.展开更多
Plutella xylostella,a major pest of cruciferous vegetables worldwide,has developed resistance to diamide insecticides.Thiotraniliprole,a novel synthetic diamide insecticide,exhibits excellent activity against P.xylost...Plutella xylostella,a major pest of cruciferous vegetables worldwide,has developed resistance to diamide insecticides.Thiotraniliprole,a novel synthetic diamide insecticide,exhibits excellent activity against P.xylostella.In the present study,we aimed to confirm the resistance risk,cross-resistance,and mechanisms of resistance to thiotraniliprole in P.xylostella.After 40 consecutive generations of thiotraniliprole selection,we obtained a thiotraniliprole-resistance P.xylostella strain with a 5141.58-fold resistance ratio(RR)to thiotraniliprole.The overall realized heritability(h^(2))value of resistance was estimated as 0.9 using threshold trait analysis,indicating that the risk of developing resistance to thiotraniliprole is high in P.xylostella.The thiotraniliprole-resistant(TR)strain showed noticeable cross-resistance to chlorantraniliprole(RR=44670.05),cyantraniliprole(RR=7038.58),and tetrachlorantraniliprole(RR=1506.01),but no cross-resistance to tolfenpyrad,indoxacarb,diafenthiuron,or abamectin compared with the susceptible(S)strain.The enzyme assay data showed that the activities of glutathione-S transferase(GST),carboxylesterase(CarE),and the content of cytochrome P450 monooxygenase(P450s)were significantly higher in the TR strain than in the S strain.Sequencing of the full-length PxRyR cDNA revealed the gene site I4790K in the TR strain with a 100%frequency.This mutation in PxRyR likely underlies the high-level cross-resistance between thiotraniliprole and three other diamide insecticides.These findings provide valuable information for optimizing resistance management strategies to delay thiotraniliprole resistance development and ensure sustainable control of P.xylostella.展开更多
Maize is an important source of calories and protein in human lives in many countries of the world and is the main staple food in Africa, particularly in eastern Africa. In the Sudan, the low yield of maize was mainly...Maize is an important source of calories and protein in human lives in many countries of the world and is the main staple food in Africa, particularly in eastern Africa. In the Sudan, the low yield of maize was mainly due to the use of low yielding landraces. It is necessary to carry out breeding programs that deal with the production of high yielding, adaptable new varieties. Therefore, this study aimed to estimate genetic variability, heritability, genotypic performance and interrelationships among the traits. Ten maize genotypes evaluated at White Nile Research Station Farm, Kosti, of the Agricultural Research Corporation (ARC), Wad Medani Sudan were planted in a randomized complete block design with three replications during the two seasons of 2021 and 2022. Most evaluated genotypes exhibited a wide and significant variation in the 11 measured traits. Genotypic coefficient of variation and genetic advance were recorded for days to 50% tasseling, ear diameter (cm), number of grains per row and grain yield (t/ha) in both seasons. High heritability and genetic advance were recorded for grain yield, ear length, ear height, plant height, number of rows per ear, ear weight, days to 50% tasseling, 100-grain weight and days to 50% silking. Moreover, there was a highly significant and positive correlation of grain yield with number of rows per ear (r = 0.479), ear length (r = 0.381), 100-grain weight (r = 0.344) and days to 50% tasseling (r = 0.214). The highest yielding five genotypes across the seasons were TZCOM1/ZDPSYN (4.2 t/ha), EEPVAH-3 (4.2 t/ha), F2TWLY131228 (4.1 t/ha), PVA SYN6F2 (3.9 t/ha) and EEPVAH-9 (3.8 t/ha) these were needed to check the adaptability, stability and to test major maize growing areas to make sound recommendations for release.展开更多
Peanut is a major cash crop in the world. In recent years, peanut pod rot has become increasingly prevalent,resulting in significant yield and quality losses. Resistance breeding is considered a cost-effective approac...Peanut is a major cash crop in the world. In recent years, peanut pod rot has become increasingly prevalent,resulting in significant yield and quality losses. Resistance breeding is considered a cost-effective approach for managing pod rot. Previous research in the United States identified significant anatomical differences in leaves and shells among peanut cultivars with varying responses to pod rot caused by Pythium myriotylum Drechs. and Rhizoctonia solani Kuhn. However, whether similar anatomical features correlate with resistance to peanut pod rot in Laixi, Qingdao, remained unknown, where the primary causal pathogen for the disease was F. solani. In this study, nine peanut varieties with differing disease reactions to peanut pod rot were planted in the same plots.Paraffin sections of leaves and shells were prepared and stained to evaluate anatomical features, and the main agronomic traits were assessed alongside leaf spot disease ratings. All the four anatomical features, leaf palisade cell number, palisade cell width, index(palisade cell number per mm × cell width in μm), and shell lignin staining area, were measured before the onset of pod rot and were found to be negatively correlated with pod rot scores.Given the consistent and strong correlation of these anatomical features with pod rot resistance and their high heritability estimates, the pre-existing resistance could be identified even in the absence of the disease, which is particularly valuable for fields where pod rot may not occur every year. This study provided useful anatomical indicators for selecting resistance to peanut pod rot, predominantly caused by F. solani.展开更多
The Pacific oyster(Crassostrea gigas)is a primary shellfish species for aquaculture around the world.However,the industry of Pacific oyster has been impaired by the mass summer mortality caused by environmental pertur...The Pacific oyster(Crassostrea gigas)is a primary shellfish species for aquaculture around the world.However,the industry of Pacific oyster has been impaired by the mass summer mortality caused by environmental perturbations,including hypoxia.Selective breeding of oysters with high hypoxia tolerance is a sustainable approach to prevent economic loss caused by hypoxia.In this study,we constructed 36 C.gigas families and executed the hypoxia stress experiment to estimate genetic parameters for hypoxia tolerance.The survival rate among 36 C.gigas families ranged from 0 to 53.33%,suggesting that high levels of phenotypic variation existed in the trait of hypoxia tolerance.Genetic parameters of hypoxia tolerance estimated by six statistical models showed a low to moderate heritability,ranging from 0.167 to 0.291.The Pearson and Spearman correlation coefficients among families estimated breeding values(EBVs)were high and positive,indicating that different models resulted in similar results in the estimation of breeding values.The phenotypic correlation between growth traits and hypoxia tolerance ranged from-0.030 to 0.061,while genetic correlation ranged from 0.110 to 0.285,implying that growth and hypoxia tolerance were independent traits.This work reported the first estimation of genetic parameters for hypoxia tolerance in C.gigas,and provided valuable information for the genetic improvement of hypoxia tolerance.展开更多
The nonlinearity and fear hypothesis predicts that highly aroused vocal mammals and birds produce vocalizations(notably alarm calls and screams)which contain a variety of nonlinear phenomena(NLP).Such vocalizations of...The nonlinearity and fear hypothesis predicts that highly aroused vocal mammals and birds produce vocalizations(notably alarm calls and screams)which contain a variety of nonlinear phenomena(NLP).Such vocalizations often sound“noisy”because vocal production systems are over-blown when animals are highly aroused.While much is known about the conditions under which animals produce vocalizations containing NLP and how species respond to them,there is little research about the heritability of such behavioral traits.Using the quantitative genetic animal model,we estimated the genetic basis of“noise”in alarm calls produced by females and found significant heritability in call entropy-our measure of the noisiness.About 9%of the variance in noisiness can be accounted for by genetic differences.Taken together,these findings suggest that the degree to which marmots produce noisy calls is modestly heritable and can be thus subject to further evolution via natural selection.展开更多
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the progressive degeneration of upper and lower motor neurons in the brainstem and spinal cord,leading to muscle weakness,para...Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the progressive degeneration of upper and lower motor neurons in the brainstem and spinal cord,leading to muscle weakness,paralysis,and respiratory failure (Morgan and Orrell,2016).展开更多
Most behavioral traits are known to be weakly heritable,possibly due to their extreme complexity and flexibility.Despite this general pattern,within-species variation in avian colony size choice has been reported to h...Most behavioral traits are known to be weakly heritable,possibly due to their extreme complexity and flexibility.Despite this general pattern,within-species variation in avian colony size choice has been reported to have a strong additive genetic component,but we are aware of no attempts to assess the heritability of avian sociality at the finer spatial scale.Here,we used an animal model and parent-offspring regression to quantify additive genetic variance in social phenotype(local nesting density)in a nonpasserine waterbird,the common tern Sterna hirundo.For this purpose,we used a novel experimental framework,where variation in the social environment was generated by providing birds with artificial patches of attractive nesting substrate that markedly varied in size.During 2011-2019,we collected data on social preferences for either low or high nesting density in over 250 individuals,either kin(mostly parent-offspring relationships)or non-kin recorded breeding multiple times across years.All heritability estimates of local nesting density were low(<0.10),irrespectively of fixed effects(sex and year)included in the models,data used in the modeling(all individuals vs.early recruits),or methodological approach(animal model vs.parent-offspring regression).We conclude that avian sociality,as measured at the local scale,may be much less heritable than colony size choice,as measured at the landscape level.Our study adds to the understanding of additive genetic variance in avian behavior,and it underlines a scale dependency in the heritability of behavioral traits.展开更多
The giant freshwater prawn Macrobrachium rosenbergii distributed from tropical to subtropical regions,is a warm-water species,and its survival temperature is 14-35°C,which greatly limits its culture cycle and cul...The giant freshwater prawn Macrobrachium rosenbergii distributed from tropical to subtropical regions,is a warm-water species,and its survival temperature is 14-35°C,which greatly limits its culture cycle and culture area in China.Therefore,it is urgent to cultivate a new high quality,high yield variety with improved cold-resistance,but the genetic parameters for cold-resistance traits are unknown in M.rosenbergii.In this study,the cold-resistance of adult M.rosenbergii populations was tested using the indoor artificial cooling method.Individuals were selected from 139 families of Shufeng G3 generation and cultured for 200 days.A linear mixed model was constructed by ASReml-R to evaluate the genetic parameters of the cold-resistance trait(cooling degree hours,CDH)and growth traits(body weight,BW,and body length,BL)based on the restricted maximum likelihood(REML)method.The results show that the heritability of CDH was low(0.12±0.05),while the growth traits(BW and BL)had low to moderate heritability,with 0.20±0.06 for BW and 0.06±0.04 for BL.The phenotypic and genetic correlation between BW and BL was significantly positive,but significantly negative phenotypic and genetic correlations were detected between CDH and BW and between CDH and BL.Furthermore,the analysis of the differences between cold-resistance and phenotypic traits showed that the female reproductive status,exoskeleton hardness and claw number of adult prawns had a great influence on the cold-resistance of M.rosenbergii(P<0.05),indicating that adults with claws and hard exoskeletons are preferred as parents in subsequent breeding selection.The present results can be attributed to the selection and breeding of a new cold-resistant variety of M.rosenbergii.展开更多
Background Thermal stress in subtropical regions is a major limiting factor in beef cattle production systems with around$369 million being lost annually due to reduced performance.Heat stress causes numerous physiolo...Background Thermal stress in subtropical regions is a major limiting factor in beef cattle production systems with around$369 million being lost annually due to reduced performance.Heat stress causes numerous physiological and behavioral disturbances including reduced feed intake and decreased production levels.Cattle utilize various physiological mechanisms such as sweating to regulate internal heat.Variation in these traits can help identify genetic variants that control sweat gland properties and subsequently allow for genetic selection of cattle with greater thermotolerance.Methods This study used 2,401 Brangus cattle from two commercial ranches in Florida.Precise phenotypes that contribute to an animal's ability to manage heat stress were calculated from skin biopsies and included sweat gland area,sweat gland depth,and sweat gland length.All animals were genotyped with the Bovine GGP F250K,and BLUPF90 software was used to estimate genetic parameters and for Genome Wide Association Study.Results Sweat gland phenotypes heritability ranged from 0.17 to 0.42 indicating a moderate amount of the phenotypic variation is due to genetics,allowing producers the ability to select for favorable sweat gland properties.A weighted single-step GWAS using sliding 10 kb windows identified multiple quantitative trait loci(QTLs)explaining a significant amount of genetic variation.QTLs located on BTA7 and BTA12 explained over 1.0%of genetic variance and overlap the ADGRV1 and CCDC168 genes,respectively.The variants identified in this study are implicated in processes related to immune function and cellular proliferation which could be relevant to heat management.Breed of Origin Alleles(BOA)were predicted using local ancestry in admixed populations(LAMP-LD),allowing for identification of markers'origin from either Brahman or Angus ancestry.A BOA GWAS was performed to identify regions inherited from particular ancestral breeds that might have a significant impact on sweat gland phenotypes.Conclusions The results of the BOA GWAS indicate that both Brahman and Angus alleles contribute positively to sweat gland traits,as evidenced by favorable marker effects observed from both genetic backgrounds.Understanding and utilizing genetic traits that confer better heat tolerance is a proactive approach to managing the impacts of climate change on livestock farming.展开更多
Diversity information mining about a crop for different attributes is an essential step for effective breeding programs.The present investigation evaluates the quantum of genetic variability and determines the relatio...Diversity information mining about a crop for different attributes is an essential step for effective breeding programs.The present investigation evaluates the quantum of genetic variability and determines the relationship among the important agro-economic traits based on two years of phenotypic data of 210 accessions of linseed.The traits,capsule weight per plant,capsule per plant,husk weight per plant,and seed weight per plant exhibited comparatively higher genetic coefficient of variation(GCV)and phenotypic coefficient of variation(PCV).In contrast,oil content and seed per capsule exhibited a lower value.The high magnitude of broad sense heritability was observed for all traits except seeds per capsule and husk weight per plant.The trait,capsules per plant,plant height,and days to 50%flowering showed high genetic advance coupled with high heritability.Hierarchical cluster analysis grouped 210 accessions into six distinct clusters.Out of 210,144(68.57%)accessions were grouped into three clusters(I,II,and III),in which cluster-III was the largest,containing 64 accessions followed by cluster II and cluster-I.The highest inter-cluster distance was observed between clusters-I and V(127.85),while the lowest was between clusters-II and IV(27.09).The positive correlation of capsule weight per plant with the seed weight per plant and a negative correlation with the days to 50%flowering indicates that high yielding linseed varieties with early flowering/maturity could be developed through direct and indirect selection.Further,seed yield and oil content could be enhanced together as indicated by ghe positive association among these two important traits.In this study,high yielding accessions with moderate to high oil content such as GP36,GP31,GP14,GP54,GP26,GP24,GP34,GP21,GP37 and GP27 and early flowering(less than 70 days)accessions such as GP2,GP26,GP27,CG33,CG44,CG42,CG132,and CG31 identified as potential genetic materials that could be exploited for developing early maturing varieties with high yield.In addition,information’s on various genetic parameters will help breeders to devise suitable breeding methodology for linseed genetic improvement for targeted traits.展开更多
Quinoa(Chenopodium quinoa Willd.)is regarded as a superfood due to its exceptionally high nutritional value and ability to withstand stress.Six quinoa genotypes(viz.,SAU Quinoa-1,Regalona,GPBQ-1,GPBQ-2,GPBQ-3,and GPBQ...Quinoa(Chenopodium quinoa Willd.)is regarded as a superfood due to its exceptionally high nutritional value and ability to withstand stress.Six quinoa genotypes(viz.,SAU Quinoa-1,Regalona,GPBQ-1,GPBQ-2,GPBQ-3,and GPBQ-4)were characterized for morphological,yield,and nutritional quality attributes while being grown under the agro-climatic conditions of Bangladesh.The field experiment was carried out in the winter season(November 2022–March 2023)at the Agronomy Field Laboratory of the Department of Agronomy,Bangladesh Agricultural University,Mymensingh,using a randomized complete block design with three replications.Data on various qualitative and quantitative traits related to growth and yield-attributes were recorded.A large variability was observed among the genotypes for growth habit,stem color,panicle shape,and panicle color at maturity.The whitish color of the seed was found in GPBQ-3 which indicates less saponin content as confirmed by saponin test.Yield attributing quantitative traits(viz.,days to first flowering,days to maturity,plant height,stem diameter,panicle weight,1000-seed weight,yield plant^(-1),above-ground biomass,and harvest index)also showed significant variation among the genotypes studied.Genotypes SAU Quinoa-1 and GPBQ-2 were identified as early maturing genotypes.The higher yield plant^(-1) was recorded in GPBQ-1 and GPBQ-3 genotypes.The phenotypic coefficient of variation for the majority of the traits evaluated was slightly higher than the corresponding genotypic coefficient of variation.For stem diameter,panicle weight,1000-seed weight,yields plant^(-1),and above-ground biomass,high heritability and high genetic advancement were seen as percentages of the mean.Yield plant^(-1) showed significant positive correlation with days to first flowering,days to maturity,plant height,stem diameter,panicle weight,and above-ground biomass(0.568*,0.812***,0.744***,0.895***,0.993***,and 0.985***,respectively).The first two components accounted for 85.5%of the overall variation among the genotypes,according to principal component analysis.Significant variability was also found for seed mineral contents(viz.,calcium,copper,iron,potassium,magnesium,manganese,and zinc)in the studied genotypes.The SAU Quinoa-1 genotype contained the highest amount of calcium and zinc,whereas,the highest amount of potassium was recorded in the GPBQ-1 genotype.We found a non-significant variability in carbohydrate,protein,fat,fiber,and vitamins(viz.,thiamine,riboflavin,niacin,and folic acid)contents in the studied genotypes.Considering all the yield and nutritional quality traits under study,the genotypes GPBQ-1,GPBQ-3,and GPBQ-4 were selected for future variety development program.展开更多
文摘Phytic acid (Myo-inositol 1,2,3,4,5,6 hexa-kisphophate) is a storage form of phosphorus and can accumulate to the levels as high as 35% in the wheat kernel. Phytic acid acts as an inhibitor for macronutrients as well as micronutrients and located in the bran of wheat kernel. Due to its inhibitory role, a high concentration of phytic acid is undesirable as it hinders the bio-availability of some essential nutrients such as Fe, Mg, Ca, Zn and Cu, etc. In order to check the inheritance of phytic acid in wheat kernels, phytic acid concentration was initially determined in kernels of 10 wheat genotypes to identify two contrasting genetic groups for diallel analysis. Based on pre-screening results of 10 wheat genotypes, five wheat genotypes (3 with high and 2 with low phytic acid concentration) were crossed in all possible combinations during 2007-2008 by 5 × 5 full diallel mating fashion to insight the inheritance of phytic acid and other yield contributing traits. All 20 F1 hybrids and five parental genotypes revealed significant differences statistically, except plant maturity. The narrow and broad sense heritability estimates varied widely among traits for spike length (0.17, 0.62), spikelets spike-1 (0.35, 0.74), tillers plant-1 (0.05, 0.52) and phytic acid concentration (0.01, 0.86). The values for phytic acid concentration ranged from 0.56% to 3.43% among F1 hybrids and 1.06 to 3.67% for parental genotypes. F1 hybrids, Ps-2005 × Ghaznavi (0.56%), AUP-4006 × Ps-2004 (0.74%), Janbaz × Ps-2004 (0.89%) and Janbaz × Ps-2005 (1.01%), had the lowest concentration of phytic acid. The study concluded that F1 hybrids with low phytic acid concentration could yield desirable segregants.
文摘The genetic resistance to angular leaf spot （ALS） caused by Phaeoisariopsis griseola in the common bean cultivar Mexico 54 was investigated on disease reactions in parental, F1, F2 and backcross generations derived from crosses between a resistant cultivar Mexico 54 and a susceptible cultivar Kablanketi under screen house conditions. The heritability （h2） estimate was as high as 0.719 indicating a successful transfer of ALS resistance among progenies and thus selection can be performed in early generation. High heritability coupled with high expected genetic advance of 39.5% is considered to be more useful in predicting the outcome of selecting the best individuals. Chi-square values were computed to determine whether the observed ratios for disease reactions deviated from expected Mendelian ratios for a single, dominant gene controlling resistance to angular leaf spot in common bean. Based on the resistance of the F2, and the backcross generation to the resistant parent, a 3 resistant： 1 susceptible segregation ratio in the F2 and a 1 resistant： 1 susceptible segregation ratio in the backcross generation to the susceptible parent was obtained implying that resistance to the isolate ofPhaeoisariopsis griseola is governed by a single, dominant gene.
基金Sponsored by Graduate Innovation Fund of Jiangxi Normal University(YJS2017044)
文摘Chinese culture has a long history. Native culture, as an important part of China's traditional culture heritage, is rich in variety. In characteristic towns, native culture has strong local characteristics, and its protection and heritage has great value. This paper aimed to inherit traditional culture, protect the local characteristics of the towns, endow the town landscape with a unique cultural value, enhance people's cultural cohesion and identity of local culture and create characteristic towns with strong cultural characteristics.
基金supported by the grants from the National Natural Science Foundation of China(Nos.31225020,31421005,and 91435206)grant from the National High Technology Research and Development of China(No.2012AA10A305)2011-G15 from the‘948’project
文摘CRISPR/Cas （clustered regularly interspaced short palindromic repeats/CR1SPR-associated proteins） is an adaptive immune system in bacteria and archaea to defend against invasion from foreign DNA fragments. Recently, it has been developed as a powerful targeted genome editing tool for a wide variety of species. However, its application in maize has only been tested with transiently expressed somatic cells or with a limited number of stable transgenic To plants. The exact efficiency and specificity of the CRISPR/Cas system in the highly complex maize genome has not been documented yet. Here we report an extensive study of the well-studied type II CRISPR-Cas9 system for targeted genome editing in maize, with the codon-optimized Cas9 protein and the short non-coding guide RNA generated through a functional maize U6 snRNA promoter. Targeted gene mutagenesis was detected for 90 loci by maize protoplast assay, with an average cleavage efficiency of 10.67 %. Stable knockout transformants for maize phytoene synthase gene （PSYI） were obtained. Mutations occurred in germ ceils can be stably inherited to the next generation. Moreover, no off-target effect was detected at the computationally predicted putative off-target loci. No significant difference between the transcriptomes of the Cas9 expressed and non-expressed lines was detected. Our results confirmed that the CRISPR-Cas9 could be successfully applied as a robust targeted genome editing system in maize.
基金funded by the Fujian Province Science and Technology Research funding for the Tree Breeding Program of Chinese fir(Min Lin 2009-4 and Min Lin Ke2013-1)Twelfth Five Year Plan in areas like national science and technology plan project(2012BAD01B0201)the Innovative Research Team of the Educational Department of China and the Innovative Research Team of the Universities of Jiangsu Province
文摘Tree growth traits （tree height, DBH and stem volume） and survival from two 9-year-old, open-pollinated progeny tests of Chinese fir were investigated for heri- tability, genotype × environment interaction, age-age genetic correlation and selection efficiency. The 97 and 79 families planted at two sites were collected from the thirdcycle seed orchard. Individual heritability was estimated between 0.05 and 0.21 for tree height, DBH, and volume and between 0.45 and 1.0 for survival. Family heritability was between 0.20 and 1.14. Significant genotype x envi- ronment interaction was observed for the three growth traits. Type B genetic correlation was between 0.41 and 0.67 with an increasing trend as tree grows. High age-age genetic correlation was observed with correlation reaching 0.9 after age 4 for height, DBH, and volume. The genetic gains were estimated at 3.26, 3.39 and 5.98 % for tree height, DBH, and volume with 10 % selection intensity. The implication for advanced tree breeding in Chinese fir is discussed.
文摘The growth performance of twenty poplar （Populus deltoides Bartr.） clones, planted in the central-plain region of Punjab in north-western India, was evaluated using a randomized block design with three replications and plot size of four plants. Significant differences among clones were observed for diameter at breast height （DBH）, height and volume per tree at age five, six and eight years. Clone L-48/89 recorded significantly superior volume than the control （G-48） at all ages, with superiority of 51.4, 43.9 and 48.5 per cent at age five, six and eight years, respectively. The phenotypic and genotypic coefficients of variation were the highest for volume （26.55-34.66% and 15.84-26.00%） and the minimum for tree height （8.43-12.13% and 4.71-7.59%）. The broad sense heritability was relatively higher for DBH （0.42-0.55） and genetic advance as per cent of mean was highest for volume （19.48-40.18）. All genetic parameters increased with age. All traits showed significant positive correlation with each other.
基金supported by the Halliburton Energy Services,Inc.,USA
文摘Guar [Cyamopsis tetragonoloba （L.） Taub.] is one of the most important industrial crops due to its richness in gum. Understanding the inheritance of gum content is a key to its successful genetic improvement. Gum content expression is reported to be controlled by additive, dominant, and epistatic effects, and modified by the environment, fg%, a relative gum content calculated by comparing with the gum content of Kinman was used as gum content （%） in this experiment. Reciprocal crosses of two lines of guar, PI 217923 and Lewis, were made to study the heritability of gum content （fg%）. fg% of four plant introductions and four commercial varieties were studied in Lubbock in 1999-2002. Estimates of broadsense heritability （h^2 b,s.） of fg% in Lewis x PI 217923 and PI 217923 x Lewis were 75.53 and 52.74%, respectively. Estimates of narrow-sense heritability （h2 n.s.） of fg% were 40.00 and 29.00% thr Lewis x PI 217923 and PI 217923 x Lewis, respectively. At least one pair of genes were estimated to control the fg% expression in these two crosses, Significant differences of fg% were found among these eight entries. PI 217923 was found to have the highest fg% among the eight entries.
文摘We have characterized the number of ovules per ovary (NoOV) in cacao plants originated from crossing the clones CCN 51 and TSH 1188 (segregating progeny) and determined the heritability of this characteristic in order to select plants with higher NoOV in this progeny. The NoOV was calculated as the average of 10 flowers per plant and ranged from 44.8 to 58.6 between the six clones (two parents and four clones belonging to their genealogy). In the progeny (n = 209 plants) the NoOV averaged 54.3 (range 44.1 to 67.8). The NoOV was distributed uniformly among the progeny indicating that this trait is conditioned by polygenes. Its heritability was estimated at 67.7%. The 32 plants with NoOV similar or superior to the genitor CCN 51 (highest amount among clones) were selected for use in the genetic improvement program. We showed that this population is suitable for genetic mapping, molecular marker identification and selection of superior cacao genotypes.
文摘From the“Six Rising Dragons”emerging at the Hangzhou Future Sci-Tech City(Haichuang Park)to the archaeological wonders of Liangzhu,widely regarded as a cradle of Chinese civilization,dating back 5000 years.Recently,our reporter has been conducting an in-depth interview in Zhejiang.Marveling at the ingenuity of ancient civilizations,we were amazed by the cutting-edge“intelligence”of humanoid robotics,and unlocked a unique dialogue between millennia-old heritage and the technology shaping tomorrow.
基金Supported by the Zhejiang Provincial Public Welfare Technology Application Research Program(No:LGN21C140001).
文摘Plutella xylostella,a major pest of cruciferous vegetables worldwide,has developed resistance to diamide insecticides.Thiotraniliprole,a novel synthetic diamide insecticide,exhibits excellent activity against P.xylostella.In the present study,we aimed to confirm the resistance risk,cross-resistance,and mechanisms of resistance to thiotraniliprole in P.xylostella.After 40 consecutive generations of thiotraniliprole selection,we obtained a thiotraniliprole-resistance P.xylostella strain with a 5141.58-fold resistance ratio(RR)to thiotraniliprole.The overall realized heritability(h^(2))value of resistance was estimated as 0.9 using threshold trait analysis,indicating that the risk of developing resistance to thiotraniliprole is high in P.xylostella.The thiotraniliprole-resistant(TR)strain showed noticeable cross-resistance to chlorantraniliprole(RR=44670.05),cyantraniliprole(RR=7038.58),and tetrachlorantraniliprole(RR=1506.01),but no cross-resistance to tolfenpyrad,indoxacarb,diafenthiuron,or abamectin compared with the susceptible(S)strain.The enzyme assay data showed that the activities of glutathione-S transferase(GST),carboxylesterase(CarE),and the content of cytochrome P450 monooxygenase(P450s)were significantly higher in the TR strain than in the S strain.Sequencing of the full-length PxRyR cDNA revealed the gene site I4790K in the TR strain with a 100%frequency.This mutation in PxRyR likely underlies the high-level cross-resistance between thiotraniliprole and three other diamide insecticides.These findings provide valuable information for optimizing resistance management strategies to delay thiotraniliprole resistance development and ensure sustainable control of P.xylostella.
文摘Maize is an important source of calories and protein in human lives in many countries of the world and is the main staple food in Africa, particularly in eastern Africa. In the Sudan, the low yield of maize was mainly due to the use of low yielding landraces. It is necessary to carry out breeding programs that deal with the production of high yielding, adaptable new varieties. Therefore, this study aimed to estimate genetic variability, heritability, genotypic performance and interrelationships among the traits. Ten maize genotypes evaluated at White Nile Research Station Farm, Kosti, of the Agricultural Research Corporation (ARC), Wad Medani Sudan were planted in a randomized complete block design with three replications during the two seasons of 2021 and 2022. Most evaluated genotypes exhibited a wide and significant variation in the 11 measured traits. Genotypic coefficient of variation and genetic advance were recorded for days to 50% tasseling, ear diameter (cm), number of grains per row and grain yield (t/ha) in both seasons. High heritability and genetic advance were recorded for grain yield, ear length, ear height, plant height, number of rows per ear, ear weight, days to 50% tasseling, 100-grain weight and days to 50% silking. Moreover, there was a highly significant and positive correlation of grain yield with number of rows per ear (r = 0.479), ear length (r = 0.381), 100-grain weight (r = 0.344) and days to 50% tasseling (r = 0.214). The highest yielding five genotypes across the seasons were TZCOM1/ZDPSYN (4.2 t/ha), EEPVAH-3 (4.2 t/ha), F2TWLY131228 (4.1 t/ha), PVA SYN6F2 (3.9 t/ha) and EEPVAH-9 (3.8 t/ha) these were needed to check the adaptability, stability and to test major maize growing areas to make sound recommendations for release.
基金support from Mining Stress Tolerant Early Maturing Peanut Genetic Resources and Breeding Processing Type Peanut Varieties in Xinjiang(2022A02008-3)China Agricultural Research System(CARS-13)+1 种基金Agricultural Science&Technology Innovation Project of Shandong Academy of Agricultural Sciences(CXGC2024D19)Key Research&Development Program of Shandong Province(2024TSGC0532).
文摘Peanut is a major cash crop in the world. In recent years, peanut pod rot has become increasingly prevalent,resulting in significant yield and quality losses. Resistance breeding is considered a cost-effective approach for managing pod rot. Previous research in the United States identified significant anatomical differences in leaves and shells among peanut cultivars with varying responses to pod rot caused by Pythium myriotylum Drechs. and Rhizoctonia solani Kuhn. However, whether similar anatomical features correlate with resistance to peanut pod rot in Laixi, Qingdao, remained unknown, where the primary causal pathogen for the disease was F. solani. In this study, nine peanut varieties with differing disease reactions to peanut pod rot were planted in the same plots.Paraffin sections of leaves and shells were prepared and stained to evaluate anatomical features, and the main agronomic traits were assessed alongside leaf spot disease ratings. All the four anatomical features, leaf palisade cell number, palisade cell width, index(palisade cell number per mm × cell width in μm), and shell lignin staining area, were measured before the onset of pod rot and were found to be negatively correlated with pod rot scores.Given the consistent and strong correlation of these anatomical features with pod rot resistance and their high heritability estimates, the pre-existing resistance could be identified even in the absence of the disease, which is particularly valuable for fields where pod rot may not occur every year. This study provided useful anatomical indicators for selecting resistance to peanut pod rot, predominantly caused by F. solani.
基金supported by the National Natural Science Foundation of China (No. 32341060)the National Key Research and Development Program of China (No.2022YFD2400300)+2 种基金the Key Research and Development Program of Shandong Province (No. 2021ZLGX03)the earmarked fund for China Agriculture Research System (CARS-49)support from the High-Performance Biological Supercomputing Center of the Ocean University of China.
文摘The Pacific oyster(Crassostrea gigas)is a primary shellfish species for aquaculture around the world.However,the industry of Pacific oyster has been impaired by the mass summer mortality caused by environmental perturbations,including hypoxia.Selective breeding of oysters with high hypoxia tolerance is a sustainable approach to prevent economic loss caused by hypoxia.In this study,we constructed 36 C.gigas families and executed the hypoxia stress experiment to estimate genetic parameters for hypoxia tolerance.The survival rate among 36 C.gigas families ranged from 0 to 53.33%,suggesting that high levels of phenotypic variation existed in the trait of hypoxia tolerance.Genetic parameters of hypoxia tolerance estimated by six statistical models showed a low to moderate heritability,ranging from 0.167 to 0.291.The Pearson and Spearman correlation coefficients among families estimated breeding values(EBVs)were high and positive,indicating that different models resulted in similar results in the estimation of breeding values.The phenotypic correlation between growth traits and hypoxia tolerance ranged from-0.030 to 0.061,while genetic correlation ranged from 0.110 to 0.285,implying that growth and hypoxia tolerance were independent traits.This work reported the first estimation of genetic parameters for hypoxia tolerance in C.gigas,and provided valuable information for the genetic improvement of hypoxia tolerance.
基金supported by the National Geographic Society,the University of California Los Angeles(Faculty Senate and Division of Life Sciences)an RMBL research fellowship and the U.S.National Science Foundation(NSF IDBR-0754247 and DEB-1119660 and 1557130 to D.T.B.,as well as DBI 0242960,07211346 and 1226713 to RMBL).
文摘The nonlinearity and fear hypothesis predicts that highly aroused vocal mammals and birds produce vocalizations(notably alarm calls and screams)which contain a variety of nonlinear phenomena(NLP).Such vocalizations often sound“noisy”because vocal production systems are over-blown when animals are highly aroused.While much is known about the conditions under which animals produce vocalizations containing NLP and how species respond to them,there is little research about the heritability of such behavioral traits.Using the quantitative genetic animal model,we estimated the genetic basis of“noise”in alarm calls produced by females and found significant heritability in call entropy-our measure of the noisiness.About 9%of the variance in noisiness can be accounted for by genetic differences.Taken together,these findings suggest that the degree to which marmots produce noisy calls is modestly heritable and can be thus subject to further evolution via natural selection.
文摘Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the progressive degeneration of upper and lower motor neurons in the brainstem and spinal cord,leading to muscle weakness,paralysis,and respiratory failure (Morgan and Orrell,2016).
基金supported by the research grant of the National Science Centre in Poland(2016/23/B/NZ8/02374).
文摘Most behavioral traits are known to be weakly heritable,possibly due to their extreme complexity and flexibility.Despite this general pattern,within-species variation in avian colony size choice has been reported to have a strong additive genetic component,but we are aware of no attempts to assess the heritability of avian sociality at the finer spatial scale.Here,we used an animal model and parent-offspring regression to quantify additive genetic variance in social phenotype(local nesting density)in a nonpasserine waterbird,the common tern Sterna hirundo.For this purpose,we used a novel experimental framework,where variation in the social environment was generated by providing birds with artificial patches of attractive nesting substrate that markedly varied in size.During 2011-2019,we collected data on social preferences for either low or high nesting density in over 250 individuals,either kin(mostly parent-offspring relationships)or non-kin recorded breeding multiple times across years.All heritability estimates of local nesting density were low(<0.10),irrespectively of fixed effects(sex and year)included in the models,data used in the modeling(all individuals vs.early recruits),or methodological approach(animal model vs.parent-offspring regression).We conclude that avian sociality,as measured at the local scale,may be much less heritable than colony size choice,as measured at the landscape level.Our study adds to the understanding of additive genetic variance in avian behavior,and it underlines a scale dependency in the heritability of behavioral traits.
基金Supported by the Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural(Aquaculture)Varieties(No.2021C02069-4-3)the Major Research&Development Program(Modern Agriculture)of Jiangsu Province(No.BE2019352)+1 种基金the Earmarked Fund for the China Agriculture Research System(No.CARS-48)the Innovation Project of Postgraduate Scientific Research in Huzhou University in 2022(No.2022KYCX63)。
文摘The giant freshwater prawn Macrobrachium rosenbergii distributed from tropical to subtropical regions,is a warm-water species,and its survival temperature is 14-35°C,which greatly limits its culture cycle and culture area in China.Therefore,it is urgent to cultivate a new high quality,high yield variety with improved cold-resistance,but the genetic parameters for cold-resistance traits are unknown in M.rosenbergii.In this study,the cold-resistance of adult M.rosenbergii populations was tested using the indoor artificial cooling method.Individuals were selected from 139 families of Shufeng G3 generation and cultured for 200 days.A linear mixed model was constructed by ASReml-R to evaluate the genetic parameters of the cold-resistance trait(cooling degree hours,CDH)and growth traits(body weight,BW,and body length,BL)based on the restricted maximum likelihood(REML)method.The results show that the heritability of CDH was low(0.12±0.05),while the growth traits(BW and BL)had low to moderate heritability,with 0.20±0.06 for BW and 0.06±0.04 for BL.The phenotypic and genetic correlation between BW and BL was significantly positive,but significantly negative phenotypic and genetic correlations were detected between CDH and BW and between CDH and BL.Furthermore,the analysis of the differences between cold-resistance and phenotypic traits showed that the female reproductive status,exoskeleton hardness and claw number of adult prawns had a great influence on the cold-resistance of M.rosenbergii(P<0.05),indicating that adults with claws and hard exoskeletons are preferred as parents in subsequent breeding selection.The present results can be attributed to the selection and breeding of a new cold-resistant variety of M.rosenbergii.
基金supported by USDA-NIFA Grants#2017–67007-26143,2020–67015-30820Florida Agricultural Experiment Station Hatch FLAANS-005548+1 种基金supported by USDA NIFA grant 2019–38420-28977Town Creek Farms。
文摘Background Thermal stress in subtropical regions is a major limiting factor in beef cattle production systems with around$369 million being lost annually due to reduced performance.Heat stress causes numerous physiological and behavioral disturbances including reduced feed intake and decreased production levels.Cattle utilize various physiological mechanisms such as sweating to regulate internal heat.Variation in these traits can help identify genetic variants that control sweat gland properties and subsequently allow for genetic selection of cattle with greater thermotolerance.Methods This study used 2,401 Brangus cattle from two commercial ranches in Florida.Precise phenotypes that contribute to an animal's ability to manage heat stress were calculated from skin biopsies and included sweat gland area,sweat gland depth,and sweat gland length.All animals were genotyped with the Bovine GGP F250K,and BLUPF90 software was used to estimate genetic parameters and for Genome Wide Association Study.Results Sweat gland phenotypes heritability ranged from 0.17 to 0.42 indicating a moderate amount of the phenotypic variation is due to genetics,allowing producers the ability to select for favorable sweat gland properties.A weighted single-step GWAS using sliding 10 kb windows identified multiple quantitative trait loci(QTLs)explaining a significant amount of genetic variation.QTLs located on BTA7 and BTA12 explained over 1.0%of genetic variance and overlap the ADGRV1 and CCDC168 genes,respectively.The variants identified in this study are implicated in processes related to immune function and cellular proliferation which could be relevant to heat management.Breed of Origin Alleles(BOA)were predicted using local ancestry in admixed populations(LAMP-LD),allowing for identification of markers'origin from either Brahman or Angus ancestry.A BOA GWAS was performed to identify regions inherited from particular ancestral breeds that might have a significant impact on sweat gland phenotypes.Conclusions The results of the BOA GWAS indicate that both Brahman and Angus alleles contribute positively to sweat gland traits,as evidenced by favorable marker effects observed from both genetic backgrounds.Understanding and utilizing genetic traits that confer better heat tolerance is a proactive approach to managing the impacts of climate change on livestock farming.
基金supported by the Department of Biotechnology,Government of India,New Delhi.Grant Number-BT/Ag/Network/Linseed/2019-20.
文摘Diversity information mining about a crop for different attributes is an essential step for effective breeding programs.The present investigation evaluates the quantum of genetic variability and determines the relationship among the important agro-economic traits based on two years of phenotypic data of 210 accessions of linseed.The traits,capsule weight per plant,capsule per plant,husk weight per plant,and seed weight per plant exhibited comparatively higher genetic coefficient of variation(GCV)and phenotypic coefficient of variation(PCV).In contrast,oil content and seed per capsule exhibited a lower value.The high magnitude of broad sense heritability was observed for all traits except seeds per capsule and husk weight per plant.The trait,capsules per plant,plant height,and days to 50%flowering showed high genetic advance coupled with high heritability.Hierarchical cluster analysis grouped 210 accessions into six distinct clusters.Out of 210,144(68.57%)accessions were grouped into three clusters(I,II,and III),in which cluster-III was the largest,containing 64 accessions followed by cluster II and cluster-I.The highest inter-cluster distance was observed between clusters-I and V(127.85),while the lowest was between clusters-II and IV(27.09).The positive correlation of capsule weight per plant with the seed weight per plant and a negative correlation with the days to 50%flowering indicates that high yielding linseed varieties with early flowering/maturity could be developed through direct and indirect selection.Further,seed yield and oil content could be enhanced together as indicated by ghe positive association among these two important traits.In this study,high yielding accessions with moderate to high oil content such as GP36,GP31,GP14,GP54,GP26,GP24,GP34,GP21,GP37 and GP27 and early flowering(less than 70 days)accessions such as GP2,GP26,GP27,CG33,CG44,CG42,CG132,and CG31 identified as potential genetic materials that could be exploited for developing early maturing varieties with high yield.In addition,information’s on various genetic parameters will help breeders to devise suitable breeding methodology for linseed genetic improvement for targeted traits.
文摘Quinoa(Chenopodium quinoa Willd.)is regarded as a superfood due to its exceptionally high nutritional value and ability to withstand stress.Six quinoa genotypes(viz.,SAU Quinoa-1,Regalona,GPBQ-1,GPBQ-2,GPBQ-3,and GPBQ-4)were characterized for morphological,yield,and nutritional quality attributes while being grown under the agro-climatic conditions of Bangladesh.The field experiment was carried out in the winter season(November 2022–March 2023)at the Agronomy Field Laboratory of the Department of Agronomy,Bangladesh Agricultural University,Mymensingh,using a randomized complete block design with three replications.Data on various qualitative and quantitative traits related to growth and yield-attributes were recorded.A large variability was observed among the genotypes for growth habit,stem color,panicle shape,and panicle color at maturity.The whitish color of the seed was found in GPBQ-3 which indicates less saponin content as confirmed by saponin test.Yield attributing quantitative traits(viz.,days to first flowering,days to maturity,plant height,stem diameter,panicle weight,1000-seed weight,yield plant^(-1),above-ground biomass,and harvest index)also showed significant variation among the genotypes studied.Genotypes SAU Quinoa-1 and GPBQ-2 were identified as early maturing genotypes.The higher yield plant^(-1) was recorded in GPBQ-1 and GPBQ-3 genotypes.The phenotypic coefficient of variation for the majority of the traits evaluated was slightly higher than the corresponding genotypic coefficient of variation.For stem diameter,panicle weight,1000-seed weight,yields plant^(-1),and above-ground biomass,high heritability and high genetic advancement were seen as percentages of the mean.Yield plant^(-1) showed significant positive correlation with days to first flowering,days to maturity,plant height,stem diameter,panicle weight,and above-ground biomass(0.568*,0.812***,0.744***,0.895***,0.993***,and 0.985***,respectively).The first two components accounted for 85.5%of the overall variation among the genotypes,according to principal component analysis.Significant variability was also found for seed mineral contents(viz.,calcium,copper,iron,potassium,magnesium,manganese,and zinc)in the studied genotypes.The SAU Quinoa-1 genotype contained the highest amount of calcium and zinc,whereas,the highest amount of potassium was recorded in the GPBQ-1 genotype.We found a non-significant variability in carbohydrate,protein,fat,fiber,and vitamins(viz.,thiamine,riboflavin,niacin,and folic acid)contents in the studied genotypes.Considering all the yield and nutritional quality traits under study,the genotypes GPBQ-1,GPBQ-3,and GPBQ-4 were selected for future variety development program.
