Graphene-based sponge is a novel hemostatic material prepared by chemical cross-link of graphene oxide. It has a fast fluid absorption capacity to quickly absorb blood from wounds, activate clotting pathways, and achi...Graphene-based sponge is a novel hemostatic material prepared by chemical cross-link of graphene oxide. It has a fast fluid absorption capacity to quickly absorb blood from wounds, activate clotting pathways, and achieve rapid hemostasis. In addition, graphene-based sponge is also a good platform carrier.It can be prepared by organic cross-linking, compounding with inorganic clay, and adding bioactive factors to enhance coagulation stimulation. By these methods, the hemostatic performance of the sponge is further improved, which shows great potential for application in the field of trauma hemostasis. This article reviews the research progress of graphene-based sponges from three different preparation strategies(organic cross-linking, inorganic compounding and adding bioactive factor), summarizes their hemostatic mechanisms, and prospects the development of graphene-based hemostatic sponges.展开更多
Hydrogel hemostatic sponges have been recognized for its effectiveness in wound treatment due to its excellent biocompatibility,degradability,as well as multi-facet functionalities.Current research focuses on optimizi...Hydrogel hemostatic sponges have been recognized for its effectiveness in wound treatment due to its excellent biocompatibility,degradability,as well as multi-facet functionalities.Current research focuses on optimizing the composition and structure of the sponge to enhance its therapeutic effectiveness.Here,we propose an adhesive hydrogel made from purely natural substances extracted from okra and Panax notoginseng.We utilize 3-dimensional(3D)printing technology to fabricate the hemostatic hydrogel scaffold,incorporating gelatin into the hydrogel and refining the mixing ratio.The interaction between gelatin and okra polyphenols contributes to successful injectability as well as stability of the printed scaffold.The okra in the scaffold exhibits favorable adhesion and hemostatic effects,and the total saponins of Panax notoginseng facilitate angiogenesis.Through in vitro experiments,we have substantiated the scaffold's excellent stability,adhesion,biocompatibility,and angiogenesis-promoting ability.Furthermore,invivo experiments have demonstrated its dual functionality in rapid hemostasis and wound repair.These features suggest that the 3D-printed,natural substance-derived hydrogel scaffolds have valuable potential in wound healing and related applications.展开更多
Uncontrolled hemorrhage remains the leading cause of death in clinical and emergency care,posing a major threat to human life.To achieve effective bleeding control,many hemostatic materials have emerged.Among them,nat...Uncontrolled hemorrhage remains the leading cause of death in clinical and emergency care,posing a major threat to human life.To achieve effective bleeding control,many hemostatic materials have emerged.Among them,nature-derived biopolymers occupy an important position due to the excellent inherent biocompatibility,biodegradability and bioactivity.Additionally,sponges have been widely used in clinical and daily life because of their rapid blood absorption.Therefore,we provide the overview focusing on the latest advances and smart designs of biopolymer-based hemostatic sponge.Starting from the component,the applications of polysaccharide and polypeptide in hemostasis are systematically introduced,and the unique bioactivities such as antibacterial,antioxidant and immunomodulation are also concerned.From the perspective of sponge structure,different preparation processes can obtain unique physical properties and structures,which will affect the material properties such as hemostasis,antibacterial and tissue repair.Notably,as development frontier,the multifunctions of hemostatic materials is summarized,mainly including enhanced coagulation,antibacterial,avoiding tumor recurrence,promoting tissue repair,and hemorrhage monitoring.Finally,the challenges facing the development of biopolymer-based hemostatic sponges are emphasized,and future directions for in vivo biosafety,emerging materials,multiple application scenarios and translational research are proposed.展开更多
Uncontrollable non-compressible hemorrhage and traumatic infection have been major causes of mortality and disability in both civilian and military populations.A dressing designed for point-of-care control of noncompr...Uncontrollable non-compressible hemorrhage and traumatic infection have been major causes of mortality and disability in both civilian and military populations.A dressing designed for point-of-care control of noncompressible hemorrhage and prevention of traumatic infections represents an urgent medical need.Here,a novel self-gelling sponge OHN@ε-pL is developed,integrating N-succinimidyl ester oxidized hyaluronic acid(OHN)andε-poly-L-lysine(ε-pL).Upon application to the wound site,the sponge can rapidly absorb interfacial fluids and undergo a phase transition from sponge to gel.The transformed gel facilitates robust tissue adhesion and achieves synergistic hemostasis by enriching coagulation factors within the sponge phase and providing a barrier effect in the gel phase.The in vitro and in vivo studies revealed that the optimized OHN@ε-pL_(3) sponge possesses self-gelling capability,tissue adhesion,enhanced coagulation ability,and exhibits excellent biocompatibility and antibacterial efficacy.In hemostasis,OHN@ε-pL_(3) sponges exhibited reduced blood loss and decreased hemostatic time compared to commercial hemostatic agents,as demonstrated in rat liver,femoral vein,and tail truncation bleeding models.Furthermore,the OHN@ε-pL_(3) sponge exhibited superior performance in accelerating wound closure and healing of S.aureus-infected wounds.Collectively,OHN@ε-pL sponges represent a promising candidate for medical dressings,specifically for managing uncontrollable noncompressible hemorrhage and traumatic infections.展开更多
Uncontrolled hemorrhage resulting from traumas causes severe health risks.There is an urgent need for expeditious hemostatic materials to treat bleeding incidents.Here,we developed a natural protein-based hemostatic s...Uncontrolled hemorrhage resulting from traumas causes severe health risks.There is an urgent need for expeditious hemostatic materials to treat bleeding incidents.Here,we developed a natural protein-based hemostatic sponge extracted from nonmulberry cocoons that exhibited rapid coagulation and effective absorption.We first built a degumming and dissolution system suitable for the Dictyoploca japonica cocoons to obtain regenerated silk fibroin(DSF).The DSF was then combined with carboxymethyl chitosan(CMCS)by glutaraldehyde(GA)crosslinking to ensure the structural stability of sponges.The resulting DSF–CMCS–GA exhibited remarkable hemostatic properties,displaying the highest absorption rate.It also demonstrated comparable efficacy to commercial hemostatic sponges.The blood-clotting index and hemolysis test showed that the prepared sponge possessed hemostatic activity and good hemocompatibility.Compared with mulberry silk fibroin hemostatic sponges(SF–CMCS–GA),DSF–CMCS–GA showed slightly better effects,making them a potential alternative to mulberry silk.In conclusion,our study introduces the use of Dictyoploca japonica silk fibroin for hemostasis,highlighting the exploitation of wild silkworm resources and providing an excellent silk fibroin-based hemostatic sealant for acute accident wounds and biomedical applications involving massive hemorrhage.展开更多
基金National Natural Science Foundation of China (No.22005020) for the financial support。
文摘Graphene-based sponge is a novel hemostatic material prepared by chemical cross-link of graphene oxide. It has a fast fluid absorption capacity to quickly absorb blood from wounds, activate clotting pathways, and achieve rapid hemostasis. In addition, graphene-based sponge is also a good platform carrier.It can be prepared by organic cross-linking, compounding with inorganic clay, and adding bioactive factors to enhance coagulation stimulation. By these methods, the hemostatic performance of the sponge is further improved, which shows great potential for application in the field of trauma hemostasis. This article reviews the research progress of graphene-based sponges from three different preparation strategies(organic cross-linking, inorganic compounding and adding bioactive factor), summarizes their hemostatic mechanisms, and prospects the development of graphene-based hemostatic sponges.
基金supported by the National Key Research and Development Program of China(2020YFA0908200)the National Natural Science Foundation of China(32271383).
文摘Hydrogel hemostatic sponges have been recognized for its effectiveness in wound treatment due to its excellent biocompatibility,degradability,as well as multi-facet functionalities.Current research focuses on optimizing the composition and structure of the sponge to enhance its therapeutic effectiveness.Here,we propose an adhesive hydrogel made from purely natural substances extracted from okra and Panax notoginseng.We utilize 3-dimensional(3D)printing technology to fabricate the hemostatic hydrogel scaffold,incorporating gelatin into the hydrogel and refining the mixing ratio.The interaction between gelatin and okra polyphenols contributes to successful injectability as well as stability of the printed scaffold.The okra in the scaffold exhibits favorable adhesion and hemostatic effects,and the total saponins of Panax notoginseng facilitate angiogenesis.Through in vitro experiments,we have substantiated the scaffold's excellent stability,adhesion,biocompatibility,and angiogenesis-promoting ability.Furthermore,invivo experiments have demonstrated its dual functionality in rapid hemostasis and wound repair.These features suggest that the 3D-printed,natural substance-derived hydrogel scaffolds have valuable potential in wound healing and related applications.
基金jointly sponsored by Sichuan Science and Tech-nology Program(2024NSFSC0002 and MZGC20240011)“1.3.5”Project for Disciplines of Excellence,West China Hospital,Sichuan University(ZYGD23037)+1 种基金the Frontiers Medical Center,Tianfu Jincheng Laboratory Foundation(TFJC2023010002)the National Natural Science Foundation of China(Grant No.32171351).
文摘Uncontrolled hemorrhage remains the leading cause of death in clinical and emergency care,posing a major threat to human life.To achieve effective bleeding control,many hemostatic materials have emerged.Among them,nature-derived biopolymers occupy an important position due to the excellent inherent biocompatibility,biodegradability and bioactivity.Additionally,sponges have been widely used in clinical and daily life because of their rapid blood absorption.Therefore,we provide the overview focusing on the latest advances and smart designs of biopolymer-based hemostatic sponge.Starting from the component,the applications of polysaccharide and polypeptide in hemostasis are systematically introduced,and the unique bioactivities such as antibacterial,antioxidant and immunomodulation are also concerned.From the perspective of sponge structure,different preparation processes can obtain unique physical properties and structures,which will affect the material properties such as hemostasis,antibacterial and tissue repair.Notably,as development frontier,the multifunctions of hemostatic materials is summarized,mainly including enhanced coagulation,antibacterial,avoiding tumor recurrence,promoting tissue repair,and hemorrhage monitoring.Finally,the challenges facing the development of biopolymer-based hemostatic sponges are emphasized,and future directions for in vivo biosafety,emerging materials,multiple application scenarios and translational research are proposed.
基金supported by the China Postdoctoral Science Foundation(Grant No.2023M740626)National Natural Science Foundation of China(Grant No.82102324)Natural Science Foundation of Chongqing(Grant No.CSTB2022BSXM-JCX0028).
文摘Uncontrollable non-compressible hemorrhage and traumatic infection have been major causes of mortality and disability in both civilian and military populations.A dressing designed for point-of-care control of noncompressible hemorrhage and prevention of traumatic infections represents an urgent medical need.Here,a novel self-gelling sponge OHN@ε-pL is developed,integrating N-succinimidyl ester oxidized hyaluronic acid(OHN)andε-poly-L-lysine(ε-pL).Upon application to the wound site,the sponge can rapidly absorb interfacial fluids and undergo a phase transition from sponge to gel.The transformed gel facilitates robust tissue adhesion and achieves synergistic hemostasis by enriching coagulation factors within the sponge phase and providing a barrier effect in the gel phase.The in vitro and in vivo studies revealed that the optimized OHN@ε-pL_(3) sponge possesses self-gelling capability,tissue adhesion,enhanced coagulation ability,and exhibits excellent biocompatibility and antibacterial efficacy.In hemostasis,OHN@ε-pL_(3) sponges exhibited reduced blood loss and decreased hemostatic time compared to commercial hemostatic agents,as demonstrated in rat liver,femoral vein,and tail truncation bleeding models.Furthermore,the OHN@ε-pL_(3) sponge exhibited superior performance in accelerating wound closure and healing of S.aureus-infected wounds.Collectively,OHN@ε-pL sponges represent a promising candidate for medical dressings,specifically for managing uncontrollable noncompressible hemorrhage and traumatic infections.
基金the National Natural Science Foundation of China(Grant Nos.51672250,32301177,and 51902289)the Key Research&Development Program of Zhejiang Province(Grant Nos.2021C01180 and 2019C04020)the Scientific Research Foundation of Zhejiang Sci-Tech University(Grant No.22212238-Y).
文摘Uncontrolled hemorrhage resulting from traumas causes severe health risks.There is an urgent need for expeditious hemostatic materials to treat bleeding incidents.Here,we developed a natural protein-based hemostatic sponge extracted from nonmulberry cocoons that exhibited rapid coagulation and effective absorption.We first built a degumming and dissolution system suitable for the Dictyoploca japonica cocoons to obtain regenerated silk fibroin(DSF).The DSF was then combined with carboxymethyl chitosan(CMCS)by glutaraldehyde(GA)crosslinking to ensure the structural stability of sponges.The resulting DSF–CMCS–GA exhibited remarkable hemostatic properties,displaying the highest absorption rate.It also demonstrated comparable efficacy to commercial hemostatic sponges.The blood-clotting index and hemolysis test showed that the prepared sponge possessed hemostatic activity and good hemocompatibility.Compared with mulberry silk fibroin hemostatic sponges(SF–CMCS–GA),DSF–CMCS–GA showed slightly better effects,making them a potential alternative to mulberry silk.In conclusion,our study introduces the use of Dictyoploca japonica silk fibroin for hemostasis,highlighting the exploitation of wild silkworm resources and providing an excellent silk fibroin-based hemostatic sealant for acute accident wounds and biomedical applications involving massive hemorrhage.
