The co-continuous(HA+β-TCP)/Zn−3Sn composite was fabricated via vacuum casting-infiltration method.The microstructure,mechanical properties,corrosion behaviors,and hemolysis ratio of the composite were studied by sca...The co-continuous(HA+β-TCP)/Zn−3Sn composite was fabricated via vacuum casting-infiltration method.The microstructure,mechanical properties,corrosion behaviors,and hemolysis ratio of the composite were studied by scanning electron microscope,X-ray diffractometer,mechanical testing,electrochemical test,immersion test,and ultraviolet spectrophotometry.The results indicate that Zn−3Sn alloy infiltrated into porous HA+β-TCP scaffold,which resulted in the formation of a compact(HA+β-TCP)/Zn−3Sn co-continuous composite,without any reaction layer between the Zn−3Sn alloy and the HA+β-TCP scaffold.The compressive strength of the composite was equal to about 3/4 that of Zn−3Sn alloy bulk.The corrosion rate of composite in simulated body fluid solution was slightly higher than that of Zn−3Sn alloy bulk.The main corrosion product on the composite surface was Zn(OH)2.The hemolysis rate of the composite was lower than that of Zn–3Sn alloy bulk and exhibited superior blood compatibility.展开更多
Hemorrhage control requires hemostatic materials that are both efective and biocompatible.Among these,diatom biosilica(DBs)could signifcantly improve hemorrhage control,but it induces hemolysis(the hemolysis rate>5...Hemorrhage control requires hemostatic materials that are both efective and biocompatible.Among these,diatom biosilica(DBs)could signifcantly improve hemorrhage control,but it induces hemolysis(the hemolysis rate>5%).Thus,the purpose of this study was to explore the infuence of Ca^(2+)biomineralization on DBs for developing fast hemostatic materials with a low hemolysis rate.Here,CaCl_(2)was added to the diatom medium under high light(cool white,fuorescent lamps,67.5µmol m^(−2) s^(−1)),producing Ca-DBs-3 with a particle size of 40-50μm and a Ca^(2+)content of Ca-DBs-3 obtained from the higher concentration CaCl_(2)group(6.7 mmol L^(−1))of 0.16%.The liquid absorption capacity of Ca-DBs-3 was 30.43±0.57 times its dry weight;the in vitro clotting time was comparable to QuikClot®zeolite;the hemostatic time and blood loss using the rat tail amputation model were 36.40±2.52 s and 0.39±0.12 g,which were 40.72%and 19.50%of QuikClot®zeolite,respectively.Ca-DBs-3 showed no apparent toxicity to L929 cells(cell viability>80%)and was non-hemolysis(the hemolysis rate<2%).This study prepared Ca-DBs-3 with a rapid hemostatic efect and good biocompatibility,providing a path to develop diatom biosilica hemostatic materials.展开更多
基金the National Natural Science Foundation of China(No.51101039)the Fundamental Research Funds for the Central Universities,China(No.3072020CFT0702).
文摘The co-continuous(HA+β-TCP)/Zn−3Sn composite was fabricated via vacuum casting-infiltration method.The microstructure,mechanical properties,corrosion behaviors,and hemolysis ratio of the composite were studied by scanning electron microscope,X-ray diffractometer,mechanical testing,electrochemical test,immersion test,and ultraviolet spectrophotometry.The results indicate that Zn−3Sn alloy infiltrated into porous HA+β-TCP scaffold,which resulted in the formation of a compact(HA+β-TCP)/Zn−3Sn co-continuous composite,without any reaction layer between the Zn−3Sn alloy and the HA+β-TCP scaffold.The compressive strength of the composite was equal to about 3/4 that of Zn−3Sn alloy bulk.The corrosion rate of composite in simulated body fluid solution was slightly higher than that of Zn−3Sn alloy bulk.The main corrosion product on the composite surface was Zn(OH)2.The hemolysis rate of the composite was lower than that of Zn–3Sn alloy bulk and exhibited superior blood compatibility.
基金The work was supported by National Natural Science Foundation of China(U22A20588,82172095)Shandong Provincial Natural Science Foundation(ZR2019QD005)Qingdao Science and Technology Demonstration and Guidance Project(20-3-4-50-nsh).
文摘Hemorrhage control requires hemostatic materials that are both efective and biocompatible.Among these,diatom biosilica(DBs)could signifcantly improve hemorrhage control,but it induces hemolysis(the hemolysis rate>5%).Thus,the purpose of this study was to explore the infuence of Ca^(2+)biomineralization on DBs for developing fast hemostatic materials with a low hemolysis rate.Here,CaCl_(2)was added to the diatom medium under high light(cool white,fuorescent lamps,67.5µmol m^(−2) s^(−1)),producing Ca-DBs-3 with a particle size of 40-50μm and a Ca^(2+)content of Ca-DBs-3 obtained from the higher concentration CaCl_(2)group(6.7 mmol L^(−1))of 0.16%.The liquid absorption capacity of Ca-DBs-3 was 30.43±0.57 times its dry weight;the in vitro clotting time was comparable to QuikClot®zeolite;the hemostatic time and blood loss using the rat tail amputation model were 36.40±2.52 s and 0.39±0.12 g,which were 40.72%and 19.50%of QuikClot®zeolite,respectively.Ca-DBs-3 showed no apparent toxicity to L929 cells(cell viability>80%)and was non-hemolysis(the hemolysis rate<2%).This study prepared Ca-DBs-3 with a rapid hemostatic efect and good biocompatibility,providing a path to develop diatom biosilica hemostatic materials.
