Atrial fibrillation(AF)is a frequent cardiac arrhythmia in the general population,which is associated with an increased risk of several health issues.It has been demonstrated that hematological variables predict the o...Atrial fibrillation(AF)is a frequent cardiac arrhythmia in the general population,which is associated with an increased risk of several health issues.It has been demonstrated that hematological variables predict the occurrence and recurrence of AF.This review article specifically only focuses on haemoglobin,hematocrit,platelet count,white blood cells(WBCs),lymphocytes,neutrophils,monocytes,neutrophil-to-lymphocyte ratio(NLR),monocyte-to-lymphocyte ratio(MLR),platelet-to-lymphocyte ratio(PLR)and red blood cells in the pathophysiology of AF.It emphasizes that there is a higher risk of new-onset AF linked with both low and high haemoglobin levels.A quantitative investigation showed that hematocrit is not linked to the development of AF.The predictive significance of platelet count was reported in nonvalvular AF patients.WBCs are consistent inflammatory markers that are associated with postoperative new-onset AF.Inflammation and in particular,leukocyte activation predisposes to AF.Enhanced migratory activity in circulating and local monocytes may play a pivotal role in the pathogenesis of progression in atrial remodeling in AF patients.In particular,the peripheral eosinophil and left atrial diameter may be important in mediating inflammation and atrial remodeling in AF.In nonvalvular AF patients,PLR may be an independent risk factor for left atrial appendage thrombogenic milieu.NLR and MLR changes are associated with early recurrence of AF,and NLR change is related to late recurrence of AF after pulmonary vein isolation.Red blood cell distribution width and left atrial dimension were the only independent risk factors associated with AF.展开更多
Lead has been recognized as a high risk toxic for most organisms including human. The effects of Pb in non-mammalian vertebrates are oprly known, particularly in anuran amphibians. The purpose of this study was to det...Lead has been recognized as a high risk toxic for most organisms including human. The effects of Pb in non-mammalian vertebrates are oprly known, particularly in anuran amphibians. The purpose of this study was to determine the effect of this metal on some hematological parameters of adult Bufo arenarum. It was found that all parameters remained unaltered within normal ranges, with the exception of reticulocyte counts which was significantly increased compared to the in controls (3. 7% vs. 0. 2 % ). It is suggested that the selective change found in reticulocyte count might be considered as an early response of a biomarker to sublethal exposition of Bufo arenarum to lead.展开更多
Diabetes mellitus(DM)is characterized by hyperglycemia and abnormalities in insulin secretion and activity.There are numerous hematological parameters;however,this review article only focuses on red blood cells,hemogl...Diabetes mellitus(DM)is characterized by hyperglycemia and abnormalities in insulin secretion and activity.There are numerous hematological parameters;however,this review article only focuses on red blood cells,hemoglobin,hematocrit,red blood cell indices,platelet count,white blood cells,lymphocytes,neutrophils,monocytes,eosinophils,neutrophil-to-lymphocyte ratio,platelet-to-lymphocyte ratio,and monocyte-to-lymphocyte ratio,which play an essential role in the pathogenesis of DM.Also,this review article aims to report the relationship between these hematological parameters and the development of DM.In con-clusion,this article shows that increased levels of platelets,red blood cells,hematocrit,lymphocytes,eosinophils,neutrophils,neutrophil-to-lymphocyte ratio,platelet-to-lymphocyte ratio,and monocyte-to-lymphocyte ratio and decreased levels of hemoglobin are involved in the pathogenesis of DM.However,the role of basophils in DM is unknown yet.展开更多
BACKGROUND Colorectal cancer(CRC)remains one of the most common malignancies worldwide,with a significant subset of patients exhibiting absence of carcinoembryonic-antigen(CEA)expression.The lack of effective diagnost...BACKGROUND Colorectal cancer(CRC)remains one of the most common malignancies worldwide,with a significant subset of patients exhibiting absence of carcinoembryonic-antigen(CEA)expression.The lack of effective diagnostic method for CEA-negative CRC prevents its early treatment.AIM To identify potentially valuable biomarkers for identifying CEA-negative CRC,the hematological characteristics of patients with CEA-negative CRC was investigated.METHODS In this retrospective analysis,74 patients were included who had been pathologically confirmed to have CEA-negative CRC,along with 79 individuals diagnosed with benign colorectal conditions.The utility of various biomarkers was evaluated using analysis of the receiver operating characteristic(ROC)curve.RESULTS Compared with patients with benign colorectal diseases,those with CEA-negative CRC had lower hemoglobin-to-red blood cell distribution width ratio(HRR)and lymphocyte-to-red blood cell distribution width ratio(LRR),and higher platelet-to-lymphocyte ratio(PLR)(P<0.05).Correlation analysis showed that HRR was negatively correlated with T stage(r=-0.237),LRR was negatively correlated with T stage(r=-0.265)and distant metastasis(r=-0.321),and PLR was positively correlated with T stage(r=0.251)(all P<0.05).ROC analysis indicated that HRR outperformed LRR and PLR in identifying CEA-negative CRC.Combining HRR and PLR provided the highest area under the curve(area under the curve=0.808;sensitivity=82.43%;specificity=68.35%)for distinguishing CEA-negative CRC from benign colorectal diseases.CONCLUSION HRR,LRR,and PLR alone or in combination could be used to distinguish CEA-negative CRC from benign colorectal diseases.展开更多
Wild rodents were collected using live snap traps in pistachio gardens of Kerman Province, Southeast Iran from 2007 to 2009, then some physiological parameters of them were measured. The samples were identified as fol...Wild rodents were collected using live snap traps in pistachio gardens of Kerman Province, Southeast Iran from 2007 to 2009, then some physiological parameters of them were measured. The samples were identified as follow: Nesokia indica, Meriones persicus, Meriones lybicus and Tatera indica. Blood samples were obtained from the heart, then the blood parameters (glucose, cholesterol, triglyceride, total protein, HDL, red and white blood cell number) in wild species of rodents and laboratory rat were compared. The results showed that there were no significant differences in serum glucose, triglyceride, HDL and total protein levels among different experimental groups. The concentration of cholesterol in T. indica was more than that in N. indica (P < 0.01). The total numbers of red blood cells also showed significant difference between wild garden rodent species and laboratory rat (P < 0.01), while the numbers of white blood cells showed no significant difference.展开更多
<b><span style="font-family:Verdana;">Introduction: </span></b><span style="font-family:;" "=""><span style="font-family:Verdana;">Sickl...<b><span style="font-family:Verdana;">Introduction: </span></b><span style="font-family:;" "=""><span style="font-family:Verdana;">Sickle cell disease is a public health problem in the Republic of Congo where the prevalence of sickle cell trait is estimated at 1.25%. The objective of this study is to describe the variations of hematological and biochemical parameters of hemolysis in sickle cell patients in critical and inter-critical periods. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> This is a descriptive cross-sectional study including sickle cell patients followed regularly at the National Reference Center for Sickle Cell Disease (CNRDr) from November 2019 to March 2020. A sample of 167 patients (sickle cell subjects in crisis and in steady state as well as control subjects) was randomly selected during the study period. The blood count was performed using a Sysmex-XN 350 automated system and the biochemical parameters were determined using the Cobas e 311 automated system. Statistical analysis was performed with SPSS version 22 software. </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> The study showed that the mean cholesterol level in controls was 4.16 ± 0.77 ul compared with 9.64 ± 4.34 ul in sickle cell crisis subjects. Hb and HCT levels were significantly higher in controls compared with sickle cell subjects in crisis. During crisis, total bilirubin, direct bilirubin, triglycerides, LDH, AST, and CRP were significantly elevated. Hematological parameters such as Hb and HCT were elevated in controls, while the mean WBC value and RET were higher in sickle cell patients in steady state. The mean values of the biochemical parameters were higher in sickle cell patients in steady state. </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> Evaluation of the influence of sickle cell trait on biochemical and hematological parameters showed significant differences between sickle cell and control subjects.</span></span>展开更多
Parameters of peripheral blood cell have been shown as the potential predictors of erectile dysfunction(ED).To investigate the clinical significance of hematological parameters for predicting the risk of rapid ejacula...Parameters of peripheral blood cell have been shown as the potential predictors of erectile dysfunction(ED).To investigate the clinical significance of hematological parameters for predicting the risk of rapid ejaculation,we established a rat copulatory model on the basis of ejaculation distribution theory.Blood samples from different ejaculatory groups were collected for peripheral blood cell counts and serum serotonin(5-HT)tests.Meanwhile,the relationship between hematological parameters and ejaculatory behaviors was assessed.Final analysis included 11 rapid ejaculators,10 normal ejaculators,and 10 sluggish ejaculators whose complete data were available.The platelet(PLT)count in rapid ejaculators was significantly lower than that in normal and sluggish ejaculators,whereas the platelet distribution width(PDW)and mean platelet volume(MPV)were significantly greater in rapid ejaculators.Multivariate logistic regression analysis and receiver operating characteristic(ROC)curve analysis showed that the PLT was an independent protective factor for rapid ejaculation.Meanwhile,rapid ejaculators were found to have the lowest serum 5-HT compared to normal and sluggish ejaculators(P<0.001).Furthermore,there was a positive correlation between the PLT and serum 5-HT(r=0.662,P<0.001),indicating that the PLT could indirectly reflect the serum 5-HT concentration.In addition,we assessed the association between the PLT and ejaculatory parameters.There was a negative correlation between ejaculation frequency(EF)and the PLT(r=−0.595,P<0.001),whereas there was a positive correlation between ejaculation latency(EL)and the PLT(r=0.740,P<0.001).This study indicated that the PLT might be a useful and convenient diagnostic marker for predicting the risk of rapid ejaculation.展开更多
Objective:To analyze the effects of Ageratum conyzoides L.on the monosodium iodoacetate induced osteoarthritis rats.Methods:Thin layer chromatography was performed to analyze the constituents of the babandotan extract...Objective:To analyze the effects of Ageratum conyzoides L.on the monosodium iodoacetate induced osteoarthritis rats.Methods:Thin layer chromatography was performed to analyze the constituents of the babandotan extract leaves.White male Sprague-Dawley rats used in this study were divided into 6 groups:normal control and negative control groups,both given0.5%carboxymethyl cellulose;the positive control group that was given glucosamine and chondroitin suspension(486 mg/200 g B.W.);the 3 dose variation extract groups including dose 1,2,and 3 that were given 40,80,and 160 mg/200 g B.W.respectively on day 29 until50.All the groups were induced with 0.05 mL monosodium iodoacetate(20 mg/mL)on day1,except normal control induced by saline.Measurement of edema volume of rat knees was performed on day 0,8,15,22,29,43,and 50.Hematology data was measured at day 1,29and 50.Serum was collected at day 50 to evaluate TNF-α and MMP-9 by ELISA.Cartilage histopathology was evaluated by staining with H&E and Safranin-o-fast green staining on day 50.Results:The babandotan leaves extract dose 2(80 mg/200 g B.W.)and dose 3(160mg/200 g B.W.)could decrease the edema volume,increase the area and thickness of articular cartilage,and increase proteoglycan level.Particularly,dose 3(160 mg/200 g B.W.)of extract babandotan leaves were able to significantly decrease the number of leukocytes,lymphocytes and udem volume,and decrease TNF alpha and MMP-9 levels.Conclusions:Babandotan leaves extract can recover inflammation and cartilages degradation by inhibiting TNF-αin inflammation processes and MMP-9 in the collagenase reaction in the cartilages.展开更多
Objective： This study investigated the acute and subacute toxicity of whole-plant aqueous extract of Vernonia mespilifolia Less. （AEVM） in rats for evaluating its safety profile. Methods： AEVM for the acute （2000...Objective： This study investigated the acute and subacute toxicity of whole-plant aqueous extract of Vernonia mespilifolia Less. （AEVM） in rats for evaluating its safety profile. Methods： AEVM for the acute （2000 and 5000 mg/kg） and subacute （200, 400 and 600 mg/kg） toxicity studies was administered orally to rats according the guidelines 425 and 407 of Organization for Economic Cooperation and Development, respectively. Food and water intake as well as body and organ weight of animals were recorded. Signs of toxicity were assessed, and hematological, biochemical and histopathological analyses were performed. Results： In the acute toxicity study, a single dose of the aqueous extract at 2000 or 5000 mg/kg caused no mortality in the animals, suggesting that the median lethal dose is greater than 5000 mg/kg. In the subacute toxicity study, administration of the extract for 28 d, at all doses, caused no significant changes in the body weights or organ weights of rats in the treated groups when compared with the control group. In addition, hematological and biochemical parameters also revealed no toxic effects of the extract on rats. Histological sections of the heart, liver and kidney from test animals showed no signs of degen- eration. Conclusion： These results showed that AEVM at dosage levels up to 600 mg/kg is nontoxic and could also offer protection on some body tissues. AEVM could, therefore, be considered safe.展开更多
Objective:To evaluate the effects of ethanol–water(80:20)extract of Marrubium vulgare(M.vulgare)on the hematological parameters,macroscopic and histological aspects of the uterus and fetus in non-pregnant and pregnan...Objective:To evaluate the effects of ethanol–water(80:20)extract of Marrubium vulgare(M.vulgare)on the hematological parameters,macroscopic and histological aspects of the uterus and fetus in non-pregnant and pregnant rats.Methods:Female rats were divided into 4 equal groups(n=9),group N(normal rats)and group G(pregnant rats)considered as control groups,group NE(normal rats treated with the ethanol–water(80:20,v/v)extract of M.vulgare)and group GE(pregnant rats treated with the extract).The ethanol–water(80:20)plant extract was administered in a single daily dose 1 g/kg at the morning,during 19 d.On the 19 day of the experiment,animals were sacrificed,the uterus and fetuses were removed for the morphological and histological studies and the blood was collected in EDTA tubes for the measurement of hematological parameters with the use of an automate‘HORIBA ABX Micros 60 Hematology Analyzer'.Results:Our results showed,in group NE and GE,a significant decrease on hematological parameters:red blood cells(NE:18.6%;GE:38.4%),hematocrit(NE:13.8%;GE:20.4%),hemoglobin(NE:12.1%;GE:8.3%)and mean corpuscular volume(NE:6.4%;GE:2%)with P more less a 0.05.Indeed,the extract of M.vulgare caused a significant decrease on the mean implantations of fetuses(82.5%,P<0.001)and their size(47.2%,P<0.01).As for the macroscopic and histological appearance of uterus,our data showed no change in normal treated rats.In contrast,the treated pregnant rats showed a severe histological change characterized by the existence of location of stopped gestation.Furthermore,it was also found in the uterus of these rat lyses placental and embryo tissue.Conclusions:All these results support the hypothesis of an abortifacient effect of M.vulgare.展开更多
The concentration of Fe（II） is high in some groundwater supplies used in turbot culture, and the toxicity of waterborne Fe（II） is unknown. We investigated the stress responses of juvenile turbot, Scophthalmus maxi...The concentration of Fe（II） is high in some groundwater supplies used in turbot culture, and the toxicity of waterborne Fe（II） is unknown. We investigated the stress responses of juvenile turbot, Scophthalmus maximus, exposed to Fe（II） of different concentrations （0.01, 0.05, 0.1, 0.5, 1, and 2 mg/L） for 1, 7, 14, and 28 d, under the same ambient conditions of other parameters. Changes in respiratory rate, hematological parameters, and gill structure were determined. The results show that waterborne Fe（II） did not cause severe hematological perturbation to turbot. A low-medium Fe（II） concentration （lower than 0.1 mg/L） could boost the respiratory rate, and caused no or very limited damage to fish. A high Fe（II） concentration （0.1 mg/L or higher）, however, caused gill damage, such as vacuoles in branchial lamellae, epithelial necrosis, and hypertrophy of epithelial cells, and even death after extended exposure time. Therefore, excess waterborne Fe（II） and long-term exposure to Fe（II） could be responsible for poor growth and high mortality of turbot in culture. The concentration of waterborne Fe（II） in turbot culture should be kept below 0.1 mg/L.展开更多
Background:Dengue fever(DF)and malaria are the two major public health concerns in tropical countries such as Thailand.Early differentiation between dengue and malaria could help clinicians to identify patients who sh...Background:Dengue fever(DF)and malaria are the two major public health concerns in tropical countries such as Thailand.Early differentiation between dengue and malaria could help clinicians to identify patients who should be closely monitored for signs of dengue hemorrhagic fever or severe malaria.This study aims to build knowledge on diagnostic markers that are used to discriminate between the infections,which frequently occur in malaria-endemic areas,such as the ones in Thailand.Methods:A retrospective study was conducted in Phop Phra Hospital,a hospital located in the Thailand-Burma border area,a malaria-endemic area,between 2013 and 2015.In brief,data on 336 patients infected with malaria were compared to data on 347 patients infected with DF.Results:White blood cells,neutrophil,monocyte,eosinophil,neutrophil-lymphocyte ratio,and monocyte-lymphocyte ratio were significantly lower in patients with DF compared to patients with malaria(P<0.0001).In contrast,red blood cells,hemoglobin,hematocrit,mean corpuscular volume,mean corpuscular hemoglobin,and mean corpuscular hemoglobin concentration were significantly higher in patients with DF as compared to patients with malaria(P<0.0001).A decision tree model revealed that using neutrophils,lymphocyte,MCHC,and gender was guided to discriminate between dengue and malaria infection.Conclusion:This study concluded that several hematological parameters were different for diagnosing DF and malaria.A decision tree model revealed that using neutrophils,lymphocyte,MCHC,and gender was guided to discriminate patients with dengue and malaria infection.In addition,using these markers will thus lead to early detection,diagnosis,and prompt treatment of these tropical diseases.展开更多
Momordica foetida is a plant widely used in tropical Africa to manage gastroenteric diseases. Previous studies demonstrated interesting antibacterial activity against human pathogenic bacteria. However, the security o...Momordica foetida is a plant widely used in tropical Africa to manage gastroenteric diseases. Previous studies demonstrated interesting antibacterial activity against human pathogenic bacteria. However, the security or toxicity of methanol leaf extract has not been determined yet. The aim of this study was to evaluate the acute and sub-acute toxicity of the leaf extract of Momordica foetida. In the acute toxicity study, a single oral dose of 5000 mg/kg body weight was administered to rats which were observed for 14 days in order to identify signs of toxicity or death. In the sub-acute toxicity, the animals were treated with 250, 500 and 1000 mg/kg of the extract for 28 consecutive days. Body weights and behavior were noted throughout the experiment. Upon treatment, blood and urine were collected for hematological and biochemical analysis. Liver, lungs, heart, kidneys, testes and ovaries were analyzed for relative weights and histopathology. The acute toxicity study of M. foetida leaf extract revealed no signs of toxicity related to the treatment, indicating that the median-lethal-dose (LD50) value is greater than 5000 mg/Kg of body weight. In the sub-acute toxicity assay, the extract did not affect the general behavior of animals, meanwhile, it led to a significant increase in the levels of red blood cells, platelets, hemoglobin, granulocytes and Mid-Cells (MIDs). Biochemical parameters showed an increase in total cholesterol, HDL cholesterol, serum urea, serum and urinary glucose and a decrease in urinary proteins, serum creatinine, urinary urea levels, serum activities of AST, ALT and proteins levels, as well as increases in lung, spleen and ovaries relative weight were noticed, all compared to control animals. Histological analysis revealed a normal architecture of kidneys, liver, heart, lung, ovaries and testes. This study provides valuable data on the safety of per os administration of Momordica foetida leaf methanol extract that could be very useful for future assays.展开更多
To evaluate the antitumor activity of Lactuca serriola against EAC （Ehrlich ascites carcinoma） in Swiss albino mice. The in vivo antitumor activity of the methanol extract of plant Lactuca serriola was evaluated at ...To evaluate the antitumor activity of Lactuca serriola against EAC （Ehrlich ascites carcinoma） in Swiss albino mice. The in vivo antitumor activity of the methanol extract of plant Lactuca serriola was evaluated at （100 mg/kg and 200 mg/kg of hole plant and 200 mg/kg, 400 mg/kg of fruit bw） against EAC using mean survival time. After administration of the extracts of Lactuca serriola, viable EAC cell count and body weight in the EAC tumor hosts were observed. The animal was also observed for improvement in the hematological parameters （e.g., hemoglobin content, red and white blood cells count and differential cell count） after treatment of plant extract. Intraperitoneal administration of plant extracts reduced viable EAC cells, increased the survival time and restored altered hematological parameters. Significant efficacy was observed for fruit extract at high concentration 400 mg/kg dose （P 〈 0.05）. It can be concluded that the methyl extract ofLactuca serriola possesses significant antitumor activity.展开更多
Bridelia micrantha, commonly known as coastal golden leaf, is a member of the family Phyllanthaceae. In preliminary studies nine fractions, named F1 - F9, were obtained by fractionating the crude methanol extract of t...Bridelia micrantha, commonly known as coastal golden leaf, is a member of the family Phyllanthaceae. In preliminary studies nine fractions, named F1 - F9, were obtained by fractionating the crude methanol extract of the stem bark of Bridelia micrantha using column chromatographic techniques. The fraction F6 was the most active when tested for antibacterial activity. Thus, toxicity of this fraction was investigated for further use. The present study evaluated the acute and sub-chronic toxicity of the crude methanolic bark extract of Bridelia micrantha and its fraction. The acute toxicity was carried out according to the experimental protocol of Organization for Economic Co-operation and Development (OECD). The plant extract or the fraction F<sub>6</sub> was administered orally to female mice at a single dose of 2000 mg/kg and the animals were observed for any behavioral changes or mortality for 14 days. In the sub-chronic toxicity study, the extract and fraction were administered orally at 200, 400 and 800 mg/kg bw/day for 28 days to healthy Wistar rats. The general behavior and body weight of the rats were recorded daily. At the end of the experimental period, hematological and biochemical analyses, changes in vital organ weight (liver, lung, heart, spleen and kidney), and histopathological examination of the liver and kidney were performed. No mortality or adverse effects were noted at the 2000 mg/kg dose during the oral acute toxicity test. In the sub-chronic study, the crude methanolic bark extract of Bridelia micrantha and the fraction F<sub>6</sub> induced no mortality or treatment-related adverse effects on body weight, general behavior, relative organ weights, hematological and biochemical parameters. Histopathological examination of the liver and kidney showed normal architecture suggesting no morphological alterations. In conclusion, the oral administration of the crude methanolic bark extract of Bridelia micrantha and the fraction F<sub>6</sub> for 28 days at a dosage of up to 800 mg/kg did not induce toxicological damage in rats. From acute toxicity study, the median lethal dose (LD<sub>50</sub>) of the crude methanolic bark extract of Bridelia micrantha and the fraction F<sub>6</sub> was estimated to be more than 2000 mg/kg.展开更多
Therapeutic efficacy of QS （quinapyramine sulphate） and FCA （Freund＇s complete adjuvant） combination was studied. The aim of the study was to evaluate therapeutic efficacy of QS using FCA in Trypanosorna congolen...Therapeutic efficacy of QS （quinapyramine sulphate） and FCA （Freund＇s complete adjuvant） combination was studied. The aim of the study was to evaluate therapeutic efficacy of QS using FCA in Trypanosorna congolense infection. GrouPs treated with QS and FCA had parasite disappeared in peripheral circulation 2 days pi, relapse was observed one week later. Effect of treatment on rectal temperature shows no significance （p 〈 0.05）, normalization of rectal temperature occurred in QS and FCA treated groups （34.1℃） than untreated （42.8 ℃）, QS （37.4 ℃） and FCA （35.92 ℃） treated groups. Mean body weight was significant （p 〈 0.001） in QS and FCA, QS, and FCA groups. Packed cell volume and hemoglobin concentration for untreated groups were lower, but increased in QS, FCA, QS and FCA treated groups, indicating anemia amelioration. White blood cell counted in untreated, QS and FCA treated groups showed no significance （p 〈 0.05）, however, there was leukocytosis due to lymphocytosis in QS and FCA treated group （6.79 × 10^3/μl） compared with untreated and other groups. There was comparative decrease in serum liver enzymes in QS and FCA treated group than other groups. Therefore, QS at lower recommended dose with FCA may enhance efficacy of QS in trypanosomiasis.展开更多
The gut microbiota undergoes substantial changes in COVID-19 patients;yet,the utility of these alterations as prognostic biomarkers at the time of hospital admission,and its correlation with immunological and hematolo...The gut microbiota undergoes substantial changes in COVID-19 patients;yet,the utility of these alterations as prognostic biomarkers at the time of hospital admission,and its correlation with immunological and hematological parameters,remains unclear.The objective of this study is to investigate the gut microbiota's dynamic change in critically ill patients with COVID-19 and evaluate its predictive capability for clinical outcomes alongside immunological and hematological parameters.In this study,anal swabs were consecutively collected from 192 COVID-19 patients(583 samples)upon hospital admission for metagenome sequencing.Simultaneously,blood samples were obtained to measure the concentrations of 27 cytokines and chemokines,along with hematological and biochemical indicators.Our findings indicate a significant correlation between the composition and dynamics of gut microbiota with disease severity and mortality in COVID-19 patients.Recovered patients exhibited a higher abundance of Veillonella and denser interactions among gut commensal bacteria compared to deceased patients.Furthermore,the abundance of gut commensal bacteria exhibited a negative correlation with the concentration of proinflammatory cytokines and organ damage markers.The gut microbiota upon admission showed moderate prognostic prediction ability with an AUC of 0.78,which was less effective compared to predictions based on immunological and hematological parameters(AUC 0.80 and 0.88,respectively).Noteworthy,the integration of these three datasets yielded a higher predictive accuracy(AUC 0.93).Our findings suggest the gut microbiota as an informative biomarker for COVID-19 prognosis,augmenting existing immune and hematological indicators.展开更多
Objective:To investigate the hematological,biochemical and immunological changes in catfish(Clarias gariepinus)(C.gariepinus)experimental exposed to cadmium.Methods:C.gariepinus were exposed to different concentration...Objective:To investigate the hematological,biochemical and immunological changes in catfish(Clarias gariepinus)(C.gariepinus)experimental exposed to cadmium.Methods:C.gariepinus were exposed to different concentrations of cadmium(Cd)(0,2,5,and 10 mg/L)for 3 weeks.Blood samples were collected for assessing some hematological,biochemical and immunological studies at the end of experiment.Results:neutrophilia and lymphopenia in 5,10 mg/L in cadmium exposed fish.Also the blood level activities of ALT and AST significantly increased,as well as glucose,creatinine,urea,potassium and uric acid.Meanwhile total protein,albumin and sodium were significantly decreased at 5,10 mg/L of cadmium exposed fish.The immunological parameters in cadmium exposed experimental dose groups decreased serum bactericidal activity,lysozyme,neutrophils adhesion test as well as decreased resistance to Aeromonas hydrophilla with increasing exposure dose seemed to correspond with suppressive of non-specific immune functions.Conclusions:The treatment of C.gariepinus with cadmium under the same conditions had immunosuppressive and decrease diseases resistance in a dose-dependent effect.展开更多
Various plant based milk extract is appropriate for human nutrition among which cottonseed is one of the potentialcrops with the advantages of stable milk emulsion, improved nutrient profile with affordable waste util...Various plant based milk extract is appropriate for human nutrition among which cottonseed is one of the potentialcrops with the advantages of stable milk emulsion, improved nutrient profile with affordable waste utilization.Although cottonseed milk is a popular indigenous beverage, it has not been exploited worldwide for regular consumptionowing to the presence of gossypol. The gossypol toxicity and associated gossypol-iron complex formationin the intestine lead to changes in hematological characteristics and alternation of sperm motility in monogastricanimals. Male Wistar albino rats weighing 60 to 70 g were divided into four groups of five animals each. The groupfed the standard diet (STD) served as control, and the experimental groups included the group (i) rats fed cottonseeddiet (CSD) supplemented at 10% of cottonseed level (ii) rats fed conventional aqueous extracted cottonseed milk(CCM) diet (CAD) and (iii) rats fed enzymatic assisted aqueous extracted cottonseed milk (ECM) diet (EAD). The CADand EAD feed was administrated @ 1 ml/100 g of animal body weight /day for the study period of 45 days among theexperimental groups and control group. A significant difference in weight gain of the experimental rats was noticedbetween the CCM and ECM cottonseed milk extracts fed experimental groups compared with the standard and cottonseed fed groups. The rats fed with CSD, CCM and ECM diet exhibited higher white blood cell counts, also reducingthe red blood cells count, hemoglobin hematocrit and platelet in the group compared with STD. No significantdifference in semen motility characteristics was noticed among the CSD, CCM and ECM fed groups. In conclusion, theintake of less than the permissible level of gossypol from selected cottonseed and its aqueous extracted milk sampleshas influenced the hematological parameters and whereas an improved effect was shown in semen characteristics.展开更多
Acute viral infections may lead to long-term adverse health effects.Investigating the hematological and biochemical profiles during recovery can provide valuable insights into the prognosis of severe fever with thromb...Acute viral infections may lead to long-term adverse health effects.Investigating the hematological and biochemical profiles during recovery can provide valuable insights into the prognosis of severe fever with thrombocytopenia syndrome(SFTS)virus infection.Herein,we performed a cross-sectional analysis of 24 hematological parameters and 12 liver and kidney function-related indicators in 143 naturally infected SFTS patients from the acute phase to 10 years post-recovery.Statistical analyses were performed using the Chi-square test(χ2),Fisher’s exact test,or the ANOVA with Bonferroni correction to assess group differences.Most indicators gradually recovered over time during the recovery period.The decrease in platelet(PLT),white blood cell,neutrophil(NEU),and lymphocyte counts in the acute phase showed a gradual recovery trend from 1-8 months to 6-10 years post-recovery.PLT count levels positively correlated significantly with recovery duration(P=0.0149).NEU%and thrombocytocrit continued to improve with the recovery time.In addition,some indicators,including platelet distribution width,mean platelet volume,and mean corpuscular hemoglobin concentration,continued to show abnormalities in a certain proportion(12.9%-69.8%)of individuals post-recovery.For liver and kidney function-related indicators,acute-phase elevations in aspartate aminotransferase and alanine aminotransferase resolved progressively.Direct bilirubin showed a gradual upward trend over time.Additionally,persistent reductions in total protein and albumin were observed in a subset of recovered individuals.These findings highlight the need for long-term monitoring of SFTS survivors and inform clinical management strategies.展开更多
文摘Atrial fibrillation(AF)is a frequent cardiac arrhythmia in the general population,which is associated with an increased risk of several health issues.It has been demonstrated that hematological variables predict the occurrence and recurrence of AF.This review article specifically only focuses on haemoglobin,hematocrit,platelet count,white blood cells(WBCs),lymphocytes,neutrophils,monocytes,neutrophil-to-lymphocyte ratio(NLR),monocyte-to-lymphocyte ratio(MLR),platelet-to-lymphocyte ratio(PLR)and red blood cells in the pathophysiology of AF.It emphasizes that there is a higher risk of new-onset AF linked with both low and high haemoglobin levels.A quantitative investigation showed that hematocrit is not linked to the development of AF.The predictive significance of platelet count was reported in nonvalvular AF patients.WBCs are consistent inflammatory markers that are associated with postoperative new-onset AF.Inflammation and in particular,leukocyte activation predisposes to AF.Enhanced migratory activity in circulating and local monocytes may play a pivotal role in the pathogenesis of progression in atrial remodeling in AF patients.In particular,the peripheral eosinophil and left atrial diameter may be important in mediating inflammation and atrial remodeling in AF.In nonvalvular AF patients,PLR may be an independent risk factor for left atrial appendage thrombogenic milieu.NLR and MLR changes are associated with early recurrence of AF,and NLR change is related to late recurrence of AF after pulmonary vein isolation.Red blood cell distribution width and left atrial dimension were the only independent risk factors associated with AF.
文摘Lead has been recognized as a high risk toxic for most organisms including human. The effects of Pb in non-mammalian vertebrates are oprly known, particularly in anuran amphibians. The purpose of this study was to determine the effect of this metal on some hematological parameters of adult Bufo arenarum. It was found that all parameters remained unaltered within normal ranges, with the exception of reticulocyte counts which was significantly increased compared to the in controls (3. 7% vs. 0. 2 % ). It is suggested that the selective change found in reticulocyte count might be considered as an early response of a biomarker to sublethal exposition of Bufo arenarum to lead.
文摘Diabetes mellitus(DM)is characterized by hyperglycemia and abnormalities in insulin secretion and activity.There are numerous hematological parameters;however,this review article only focuses on red blood cells,hemoglobin,hematocrit,red blood cell indices,platelet count,white blood cells,lymphocytes,neutrophils,monocytes,eosinophils,neutrophil-to-lymphocyte ratio,platelet-to-lymphocyte ratio,and monocyte-to-lymphocyte ratio,which play an essential role in the pathogenesis of DM.Also,this review article aims to report the relationship between these hematological parameters and the development of DM.In con-clusion,this article shows that increased levels of platelets,red blood cells,hematocrit,lymphocytes,eosinophils,neutrophils,neutrophil-to-lymphocyte ratio,platelet-to-lymphocyte ratio,and monocyte-to-lymphocyte ratio and decreased levels of hemoglobin are involved in the pathogenesis of DM.However,the role of basophils in DM is unknown yet.
基金Supported by Youth Project of Guangxi International Zhuang Medicine Hospital,No.[2022]203Discipline Project of Guangxi International Zhuang Medicine Hospital,No.[2021]33Research Fund of Guangxi International Zhuang Medicine Hospital,No.RCYJ202201.
文摘BACKGROUND Colorectal cancer(CRC)remains one of the most common malignancies worldwide,with a significant subset of patients exhibiting absence of carcinoembryonic-antigen(CEA)expression.The lack of effective diagnostic method for CEA-negative CRC prevents its early treatment.AIM To identify potentially valuable biomarkers for identifying CEA-negative CRC,the hematological characteristics of patients with CEA-negative CRC was investigated.METHODS In this retrospective analysis,74 patients were included who had been pathologically confirmed to have CEA-negative CRC,along with 79 individuals diagnosed with benign colorectal conditions.The utility of various biomarkers was evaluated using analysis of the receiver operating characteristic(ROC)curve.RESULTS Compared with patients with benign colorectal diseases,those with CEA-negative CRC had lower hemoglobin-to-red blood cell distribution width ratio(HRR)and lymphocyte-to-red blood cell distribution width ratio(LRR),and higher platelet-to-lymphocyte ratio(PLR)(P<0.05).Correlation analysis showed that HRR was negatively correlated with T stage(r=-0.237),LRR was negatively correlated with T stage(r=-0.265)and distant metastasis(r=-0.321),and PLR was positively correlated with T stage(r=0.251)(all P<0.05).ROC analysis indicated that HRR outperformed LRR and PLR in identifying CEA-negative CRC.Combining HRR and PLR provided the highest area under the curve(area under the curve=0.808;sensitivity=82.43%;specificity=68.35%)for distinguishing CEA-negative CRC from benign colorectal diseases.CONCLUSION HRR,LRR,and PLR alone or in combination could be used to distinguish CEA-negative CRC from benign colorectal diseases.
文摘Wild rodents were collected using live snap traps in pistachio gardens of Kerman Province, Southeast Iran from 2007 to 2009, then some physiological parameters of them were measured. The samples were identified as follow: Nesokia indica, Meriones persicus, Meriones lybicus and Tatera indica. Blood samples were obtained from the heart, then the blood parameters (glucose, cholesterol, triglyceride, total protein, HDL, red and white blood cell number) in wild species of rodents and laboratory rat were compared. The results showed that there were no significant differences in serum glucose, triglyceride, HDL and total protein levels among different experimental groups. The concentration of cholesterol in T. indica was more than that in N. indica (P < 0.01). The total numbers of red blood cells also showed significant difference between wild garden rodent species and laboratory rat (P < 0.01), while the numbers of white blood cells showed no significant difference.
文摘<b><span style="font-family:Verdana;">Introduction: </span></b><span style="font-family:;" "=""><span style="font-family:Verdana;">Sickle cell disease is a public health problem in the Republic of Congo where the prevalence of sickle cell trait is estimated at 1.25%. The objective of this study is to describe the variations of hematological and biochemical parameters of hemolysis in sickle cell patients in critical and inter-critical periods. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> This is a descriptive cross-sectional study including sickle cell patients followed regularly at the National Reference Center for Sickle Cell Disease (CNRDr) from November 2019 to March 2020. A sample of 167 patients (sickle cell subjects in crisis and in steady state as well as control subjects) was randomly selected during the study period. The blood count was performed using a Sysmex-XN 350 automated system and the biochemical parameters were determined using the Cobas e 311 automated system. Statistical analysis was performed with SPSS version 22 software. </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> The study showed that the mean cholesterol level in controls was 4.16 ± 0.77 ul compared with 9.64 ± 4.34 ul in sickle cell crisis subjects. Hb and HCT levels were significantly higher in controls compared with sickle cell subjects in crisis. During crisis, total bilirubin, direct bilirubin, triglycerides, LDH, AST, and CRP were significantly elevated. Hematological parameters such as Hb and HCT were elevated in controls, while the mean WBC value and RET were higher in sickle cell patients in steady state. The mean values of the biochemical parameters were higher in sickle cell patients in steady state. </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> Evaluation of the influence of sickle cell trait on biochemical and hematological parameters showed significant differences between sickle cell and control subjects.</span></span>
基金funded by the Research Foundation of Anhui Provincial Institute of Translational Medicine(No.2023zhyx-C94)the National Natural Science Foundation of China(No.82071637).
文摘Parameters of peripheral blood cell have been shown as the potential predictors of erectile dysfunction(ED).To investigate the clinical significance of hematological parameters for predicting the risk of rapid ejaculation,we established a rat copulatory model on the basis of ejaculation distribution theory.Blood samples from different ejaculatory groups were collected for peripheral blood cell counts and serum serotonin(5-HT)tests.Meanwhile,the relationship between hematological parameters and ejaculatory behaviors was assessed.Final analysis included 11 rapid ejaculators,10 normal ejaculators,and 10 sluggish ejaculators whose complete data were available.The platelet(PLT)count in rapid ejaculators was significantly lower than that in normal and sluggish ejaculators,whereas the platelet distribution width(PDW)and mean platelet volume(MPV)were significantly greater in rapid ejaculators.Multivariate logistic regression analysis and receiver operating characteristic(ROC)curve analysis showed that the PLT was an independent protective factor for rapid ejaculation.Meanwhile,rapid ejaculators were found to have the lowest serum 5-HT compared to normal and sluggish ejaculators(P<0.001).Furthermore,there was a positive correlation between the PLT and serum 5-HT(r=0.662,P<0.001),indicating that the PLT could indirectly reflect the serum 5-HT concentration.In addition,we assessed the association between the PLT and ejaculatory parameters.There was a negative correlation between ejaculation frequency(EF)and the PLT(r=−0.595,P<0.001),whereas there was a positive correlation between ejaculation latency(EL)and the PLT(r=0.740,P<0.001).This study indicated that the PLT might be a useful and convenient diagnostic marker for predicting the risk of rapid ejaculation.
基金supported by the Ministry of Research Technology and Higher Education of Indonesia 2016
文摘Objective:To analyze the effects of Ageratum conyzoides L.on the monosodium iodoacetate induced osteoarthritis rats.Methods:Thin layer chromatography was performed to analyze the constituents of the babandotan extract leaves.White male Sprague-Dawley rats used in this study were divided into 6 groups:normal control and negative control groups,both given0.5%carboxymethyl cellulose;the positive control group that was given glucosamine and chondroitin suspension(486 mg/200 g B.W.);the 3 dose variation extract groups including dose 1,2,and 3 that were given 40,80,and 160 mg/200 g B.W.respectively on day 29 until50.All the groups were induced with 0.05 mL monosodium iodoacetate(20 mg/mL)on day1,except normal control induced by saline.Measurement of edema volume of rat knees was performed on day 0,8,15,22,29,43,and 50.Hematology data was measured at day 1,29and 50.Serum was collected at day 50 to evaluate TNF-α and MMP-9 by ELISA.Cartilage histopathology was evaluated by staining with H&E and Safranin-o-fast green staining on day 50.Results:The babandotan leaves extract dose 2(80 mg/200 g B.W.)and dose 3(160mg/200 g B.W.)could decrease the edema volume,increase the area and thickness of articular cartilage,and increase proteoglycan level.Particularly,dose 3(160 mg/200 g B.W.)of extract babandotan leaves were able to significantly decrease the number of leukocytes,lymphocytes and udem volume,and decrease TNF alpha and MMP-9 levels.Conclusions:Babandotan leaves extract can recover inflammation and cartilages degradation by inhibiting TNF-αin inflammation processes and MMP-9 in the collagenase reaction in the cartilages.
基金the financial support of Govan Mbeki Research Development Centre, University of Fort Hare, South Africa.Grant number C127
文摘Objective： This study investigated the acute and subacute toxicity of whole-plant aqueous extract of Vernonia mespilifolia Less. （AEVM） in rats for evaluating its safety profile. Methods： AEVM for the acute （2000 and 5000 mg/kg） and subacute （200, 400 and 600 mg/kg） toxicity studies was administered orally to rats according the guidelines 425 and 407 of Organization for Economic Cooperation and Development, respectively. Food and water intake as well as body and organ weight of animals were recorded. Signs of toxicity were assessed, and hematological, biochemical and histopathological analyses were performed. Results： In the acute toxicity study, a single dose of the aqueous extract at 2000 or 5000 mg/kg caused no mortality in the animals, suggesting that the median lethal dose is greater than 5000 mg/kg. In the subacute toxicity study, administration of the extract for 28 d, at all doses, caused no significant changes in the body weights or organ weights of rats in the treated groups when compared with the control group. In addition, hematological and biochemical parameters also revealed no toxic effects of the extract on rats. Histological sections of the heart, liver and kidney from test animals showed no signs of degen- eration. Conclusion： These results showed that AEVM at dosage levels up to 600 mg/kg is nontoxic and could also offer protection on some body tissues. AEVM could, therefore, be considered safe.
基金supported by grants from the Ministry of Higher Education and Research,was conducted in laboratory of research:Monitoring and nutritional epidemiology in Tunisia,LR 12SP05
文摘Objective:To evaluate the effects of ethanol–water(80:20)extract of Marrubium vulgare(M.vulgare)on the hematological parameters,macroscopic and histological aspects of the uterus and fetus in non-pregnant and pregnant rats.Methods:Female rats were divided into 4 equal groups(n=9),group N(normal rats)and group G(pregnant rats)considered as control groups,group NE(normal rats treated with the ethanol–water(80:20,v/v)extract of M.vulgare)and group GE(pregnant rats treated with the extract).The ethanol–water(80:20)plant extract was administered in a single daily dose 1 g/kg at the morning,during 19 d.On the 19 day of the experiment,animals were sacrificed,the uterus and fetuses were removed for the morphological and histological studies and the blood was collected in EDTA tubes for the measurement of hematological parameters with the use of an automate‘HORIBA ABX Micros 60 Hematology Analyzer'.Results:Our results showed,in group NE and GE,a significant decrease on hematological parameters:red blood cells(NE:18.6%;GE:38.4%),hematocrit(NE:13.8%;GE:20.4%),hemoglobin(NE:12.1%;GE:8.3%)and mean corpuscular volume(NE:6.4%;GE:2%)with P more less a 0.05.Indeed,the extract of M.vulgare caused a significant decrease on the mean implantations of fetuses(82.5%,P<0.001)and their size(47.2%,P<0.01).As for the macroscopic and histological appearance of uterus,our data showed no change in normal treated rats.In contrast,the treated pregnant rats showed a severe histological change characterized by the existence of location of stopped gestation.Furthermore,it was also found in the uterus of these rat lyses placental and embryo tissue.Conclusions:All these results support the hypothesis of an abortifacient effect of M.vulgare.
基金Supported by the National Key Technology R&D Program(Nos.2011BAD13B04,2006BAD09A11)Shandong Agricultural Seed Stock Breeding Project
文摘The concentration of Fe（II） is high in some groundwater supplies used in turbot culture, and the toxicity of waterborne Fe（II） is unknown. We investigated the stress responses of juvenile turbot, Scophthalmus maximus, exposed to Fe（II） of different concentrations （0.01, 0.05, 0.1, 0.5, 1, and 2 mg/L） for 1, 7, 14, and 28 d, under the same ambient conditions of other parameters. Changes in respiratory rate, hematological parameters, and gill structure were determined. The results show that waterborne Fe（II） did not cause severe hematological perturbation to turbot. A low-medium Fe（II） concentration （lower than 0.1 mg/L） could boost the respiratory rate, and caused no or very limited damage to fish. A high Fe（II） concentration （0.1 mg/L or higher）, however, caused gill damage, such as vacuoles in branchial lamellae, epithelial necrosis, and hypertrophy of epithelial cells, and even death after extended exposure time. Therefore, excess waterborne Fe（II） and long-term exposure to Fe（II） could be responsible for poor growth and high mortality of turbot in culture. The concentration of waterborne Fe（II） in turbot culture should be kept below 0.1 mg/L.
基金This research was funded by grants from Walailak University(Predictive Risk Factors for Diseases and Health Impact Research Group,WU58525).
文摘Background:Dengue fever(DF)and malaria are the two major public health concerns in tropical countries such as Thailand.Early differentiation between dengue and malaria could help clinicians to identify patients who should be closely monitored for signs of dengue hemorrhagic fever or severe malaria.This study aims to build knowledge on diagnostic markers that are used to discriminate between the infections,which frequently occur in malaria-endemic areas,such as the ones in Thailand.Methods:A retrospective study was conducted in Phop Phra Hospital,a hospital located in the Thailand-Burma border area,a malaria-endemic area,between 2013 and 2015.In brief,data on 336 patients infected with malaria were compared to data on 347 patients infected with DF.Results:White blood cells,neutrophil,monocyte,eosinophil,neutrophil-lymphocyte ratio,and monocyte-lymphocyte ratio were significantly lower in patients with DF compared to patients with malaria(P<0.0001).In contrast,red blood cells,hemoglobin,hematocrit,mean corpuscular volume,mean corpuscular hemoglobin,and mean corpuscular hemoglobin concentration were significantly higher in patients with DF as compared to patients with malaria(P<0.0001).A decision tree model revealed that using neutrophils,lymphocyte,MCHC,and gender was guided to discriminate between dengue and malaria infection.Conclusion:This study concluded that several hematological parameters were different for diagnosing DF and malaria.A decision tree model revealed that using neutrophils,lymphocyte,MCHC,and gender was guided to discriminate patients with dengue and malaria infection.In addition,using these markers will thus lead to early detection,diagnosis,and prompt treatment of these tropical diseases.
文摘Momordica foetida is a plant widely used in tropical Africa to manage gastroenteric diseases. Previous studies demonstrated interesting antibacterial activity against human pathogenic bacteria. However, the security or toxicity of methanol leaf extract has not been determined yet. The aim of this study was to evaluate the acute and sub-acute toxicity of the leaf extract of Momordica foetida. In the acute toxicity study, a single oral dose of 5000 mg/kg body weight was administered to rats which were observed for 14 days in order to identify signs of toxicity or death. In the sub-acute toxicity, the animals were treated with 250, 500 and 1000 mg/kg of the extract for 28 consecutive days. Body weights and behavior were noted throughout the experiment. Upon treatment, blood and urine were collected for hematological and biochemical analysis. Liver, lungs, heart, kidneys, testes and ovaries were analyzed for relative weights and histopathology. The acute toxicity study of M. foetida leaf extract revealed no signs of toxicity related to the treatment, indicating that the median-lethal-dose (LD50) value is greater than 5000 mg/Kg of body weight. In the sub-acute toxicity assay, the extract did not affect the general behavior of animals, meanwhile, it led to a significant increase in the levels of red blood cells, platelets, hemoglobin, granulocytes and Mid-Cells (MIDs). Biochemical parameters showed an increase in total cholesterol, HDL cholesterol, serum urea, serum and urinary glucose and a decrease in urinary proteins, serum creatinine, urinary urea levels, serum activities of AST, ALT and proteins levels, as well as increases in lung, spleen and ovaries relative weight were noticed, all compared to control animals. Histological analysis revealed a normal architecture of kidneys, liver, heart, lung, ovaries and testes. This study provides valuable data on the safety of per os administration of Momordica foetida leaf methanol extract that could be very useful for future assays.
文摘To evaluate the antitumor activity of Lactuca serriola against EAC （Ehrlich ascites carcinoma） in Swiss albino mice. The in vivo antitumor activity of the methanol extract of plant Lactuca serriola was evaluated at （100 mg/kg and 200 mg/kg of hole plant and 200 mg/kg, 400 mg/kg of fruit bw） against EAC using mean survival time. After administration of the extracts of Lactuca serriola, viable EAC cell count and body weight in the EAC tumor hosts were observed. The animal was also observed for improvement in the hematological parameters （e.g., hemoglobin content, red and white blood cells count and differential cell count） after treatment of plant extract. Intraperitoneal administration of plant extracts reduced viable EAC cells, increased the survival time and restored altered hematological parameters. Significant efficacy was observed for fruit extract at high concentration 400 mg/kg dose （P 〈 0.05）. It can be concluded that the methyl extract ofLactuca serriola possesses significant antitumor activity.
文摘Bridelia micrantha, commonly known as coastal golden leaf, is a member of the family Phyllanthaceae. In preliminary studies nine fractions, named F1 - F9, were obtained by fractionating the crude methanol extract of the stem bark of Bridelia micrantha using column chromatographic techniques. The fraction F6 was the most active when tested for antibacterial activity. Thus, toxicity of this fraction was investigated for further use. The present study evaluated the acute and sub-chronic toxicity of the crude methanolic bark extract of Bridelia micrantha and its fraction. The acute toxicity was carried out according to the experimental protocol of Organization for Economic Co-operation and Development (OECD). The plant extract or the fraction F<sub>6</sub> was administered orally to female mice at a single dose of 2000 mg/kg and the animals were observed for any behavioral changes or mortality for 14 days. In the sub-chronic toxicity study, the extract and fraction were administered orally at 200, 400 and 800 mg/kg bw/day for 28 days to healthy Wistar rats. The general behavior and body weight of the rats were recorded daily. At the end of the experimental period, hematological and biochemical analyses, changes in vital organ weight (liver, lung, heart, spleen and kidney), and histopathological examination of the liver and kidney were performed. No mortality or adverse effects were noted at the 2000 mg/kg dose during the oral acute toxicity test. In the sub-chronic study, the crude methanolic bark extract of Bridelia micrantha and the fraction F<sub>6</sub> induced no mortality or treatment-related adverse effects on body weight, general behavior, relative organ weights, hematological and biochemical parameters. Histopathological examination of the liver and kidney showed normal architecture suggesting no morphological alterations. In conclusion, the oral administration of the crude methanolic bark extract of Bridelia micrantha and the fraction F<sub>6</sub> for 28 days at a dosage of up to 800 mg/kg did not induce toxicological damage in rats. From acute toxicity study, the median lethal dose (LD<sub>50</sub>) of the crude methanolic bark extract of Bridelia micrantha and the fraction F<sub>6</sub> was estimated to be more than 2000 mg/kg.
文摘Therapeutic efficacy of QS （quinapyramine sulphate） and FCA （Freund＇s complete adjuvant） combination was studied. The aim of the study was to evaluate therapeutic efficacy of QS using FCA in Trypanosorna congolense infection. GrouPs treated with QS and FCA had parasite disappeared in peripheral circulation 2 days pi, relapse was observed one week later. Effect of treatment on rectal temperature shows no significance （p 〈 0.05）, normalization of rectal temperature occurred in QS and FCA treated groups （34.1℃） than untreated （42.8 ℃）, QS （37.4 ℃） and FCA （35.92 ℃） treated groups. Mean body weight was significant （p 〈 0.001） in QS and FCA, QS, and FCA groups. Packed cell volume and hemoglobin concentration for untreated groups were lower, but increased in QS, FCA, QS and FCA treated groups, indicating anemia amelioration. White blood cell counted in untreated, QS and FCA treated groups showed no significance （p 〈 0.05）, however, there was leukocytosis due to lymphocytosis in QS and FCA treated group （6.79 × 10^3/μl） compared with untreated and other groups. There was comparative decrease in serum liver enzymes in QS and FCA treated group than other groups. Therefore, QS at lower recommended dose with FCA may enhance efficacy of QS in trypanosomiasis.
基金supported by the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences(2019PT310029)Chinese Academy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(2023-I2M-2-001)+3 种基金the National Natural Science Foundation of China(82161148009)the Fundamental Research Funds for the Central Universities(3332021092)the Strategic Priority Research Program of Chinese Academy of Sciences(grant no.XDB38030400)the Key Collaborative Research Program of the Alliance of International Science Organizations(ANSO-CR-KP-2022-09).
文摘The gut microbiota undergoes substantial changes in COVID-19 patients;yet,the utility of these alterations as prognostic biomarkers at the time of hospital admission,and its correlation with immunological and hematological parameters,remains unclear.The objective of this study is to investigate the gut microbiota's dynamic change in critically ill patients with COVID-19 and evaluate its predictive capability for clinical outcomes alongside immunological and hematological parameters.In this study,anal swabs were consecutively collected from 192 COVID-19 patients(583 samples)upon hospital admission for metagenome sequencing.Simultaneously,blood samples were obtained to measure the concentrations of 27 cytokines and chemokines,along with hematological and biochemical indicators.Our findings indicate a significant correlation between the composition and dynamics of gut microbiota with disease severity and mortality in COVID-19 patients.Recovered patients exhibited a higher abundance of Veillonella and denser interactions among gut commensal bacteria compared to deceased patients.Furthermore,the abundance of gut commensal bacteria exhibited a negative correlation with the concentration of proinflammatory cytokines and organ damage markers.The gut microbiota upon admission showed moderate prognostic prediction ability with an AUC of 0.78,which was less effective compared to predictions based on immunological and hematological parameters(AUC 0.80 and 0.88,respectively).Noteworthy,the integration of these three datasets yielded a higher predictive accuracy(AUC 0.93).Our findings suggest the gut microbiota as an informative biomarker for COVID-19 prognosis,augmenting existing immune and hematological indicators.
文摘Objective:To investigate the hematological,biochemical and immunological changes in catfish(Clarias gariepinus)(C.gariepinus)experimental exposed to cadmium.Methods:C.gariepinus were exposed to different concentrations of cadmium(Cd)(0,2,5,and 10 mg/L)for 3 weeks.Blood samples were collected for assessing some hematological,biochemical and immunological studies at the end of experiment.Results:neutrophilia and lymphopenia in 5,10 mg/L in cadmium exposed fish.Also the blood level activities of ALT and AST significantly increased,as well as glucose,creatinine,urea,potassium and uric acid.Meanwhile total protein,albumin and sodium were significantly decreased at 5,10 mg/L of cadmium exposed fish.The immunological parameters in cadmium exposed experimental dose groups decreased serum bactericidal activity,lysozyme,neutrophils adhesion test as well as decreased resistance to Aeromonas hydrophilla with increasing exposure dose seemed to correspond with suppressive of non-specific immune functions.Conclusions:The treatment of C.gariepinus with cadmium under the same conditions had immunosuppressive and decrease diseases resistance in a dose-dependent effect.
文摘Various plant based milk extract is appropriate for human nutrition among which cottonseed is one of the potentialcrops with the advantages of stable milk emulsion, improved nutrient profile with affordable waste utilization.Although cottonseed milk is a popular indigenous beverage, it has not been exploited worldwide for regular consumptionowing to the presence of gossypol. The gossypol toxicity and associated gossypol-iron complex formationin the intestine lead to changes in hematological characteristics and alternation of sperm motility in monogastricanimals. Male Wistar albino rats weighing 60 to 70 g were divided into four groups of five animals each. The groupfed the standard diet (STD) served as control, and the experimental groups included the group (i) rats fed cottonseeddiet (CSD) supplemented at 10% of cottonseed level (ii) rats fed conventional aqueous extracted cottonseed milk(CCM) diet (CAD) and (iii) rats fed enzymatic assisted aqueous extracted cottonseed milk (ECM) diet (EAD). The CADand EAD feed was administrated @ 1 ml/100 g of animal body weight /day for the study period of 45 days among theexperimental groups and control group. A significant difference in weight gain of the experimental rats was noticedbetween the CCM and ECM cottonseed milk extracts fed experimental groups compared with the standard and cottonseed fed groups. The rats fed with CSD, CCM and ECM diet exhibited higher white blood cell counts, also reducingthe red blood cells count, hemoglobin hematocrit and platelet in the group compared with STD. No significantdifference in semen motility characteristics was noticed among the CSD, CCM and ECM fed groups. In conclusion, theintake of less than the permissible level of gossypol from selected cottonseed and its aqueous extracted milk sampleshas influenced the hematological parameters and whereas an improved effect was shown in semen characteristics.
基金supported by the National Key Research and Development Program of China(2022YFC2604100)the National Natural Science Foundation of China(92269203)the R&D Program of Guangzhou National Laboratory(SRPG23-005).
文摘Acute viral infections may lead to long-term adverse health effects.Investigating the hematological and biochemical profiles during recovery can provide valuable insights into the prognosis of severe fever with thrombocytopenia syndrome(SFTS)virus infection.Herein,we performed a cross-sectional analysis of 24 hematological parameters and 12 liver and kidney function-related indicators in 143 naturally infected SFTS patients from the acute phase to 10 years post-recovery.Statistical analyses were performed using the Chi-square test(χ2),Fisher’s exact test,or the ANOVA with Bonferroni correction to assess group differences.Most indicators gradually recovered over time during the recovery period.The decrease in platelet(PLT),white blood cell,neutrophil(NEU),and lymphocyte counts in the acute phase showed a gradual recovery trend from 1-8 months to 6-10 years post-recovery.PLT count levels positively correlated significantly with recovery duration(P=0.0149).NEU%and thrombocytocrit continued to improve with the recovery time.In addition,some indicators,including platelet distribution width,mean platelet volume,and mean corpuscular hemoglobin concentration,continued to show abnormalities in a certain proportion(12.9%-69.8%)of individuals post-recovery.For liver and kidney function-related indicators,acute-phase elevations in aspartate aminotransferase and alanine aminotransferase resolved progressively.Direct bilirubin showed a gradual upward trend over time.Additionally,persistent reductions in total protein and albumin were observed in a subset of recovered individuals.These findings highlight the need for long-term monitoring of SFTS survivors and inform clinical management strategies.
