[Objectives]To establish a high-performance liquid chromatography(HPLC)fingerprint analysis method for Hedyotis hedyotidea,providing a scientific basis for its quality evaluation.[Methods]An Agilent ZORBAX SB-C_(18)co...[Objectives]To establish a high-performance liquid chromatography(HPLC)fingerprint analysis method for Hedyotis hedyotidea,providing a scientific basis for its quality evaluation.[Methods]An Agilent ZORBAX SB-C_(18)column(5μm,4.6 mm×250 mm)was employed with 0.1%phosphoric acid-acetonitrile solution as the mobile phase for gradient elution.The column temperature was maintained at 40℃,detection wavelength at 240 nm,flow rate at 0.8 mL/min,and injection volume at 10μL.The Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(2004A edition)was used to analyze the HPLC fingerprints of 10 batches of H.hedyotidea samples.[Results]An HPLC fingerprint with ursolic acid as the reference standard was established,identifying 17 common characteristic peaks.The similarity of the 10 batches of H.hedyotidea samples ranged from 0.718 to 0.993.[Conclusions]This method demonstrates good systematic performance,with precision(RSD<1.5%),repeatability(RSD<2.0%),and 24-h stability(RSD<2.5%)meeting established requirements.It can provide data support for quality control and standard formulation of H.hedyotidea medicinal materials.展开更多
[Objectives]This study was conducted to investigate the antibacterial effect of the extract from Hedyotis hedyotidea(DC)Merr.[Methods]Through in-vitro antibacterial tests,the antibacterial effects of H.hedyotidea etha...[Objectives]This study was conducted to investigate the antibacterial effect of the extract from Hedyotis hedyotidea(DC)Merr.[Methods]Through in-vitro antibacterial tests,the antibacterial effects of H.hedyotidea ethanol extract on Escherichia coli,Staphylococcus aureus and Bacillus subtilis were analyzed.[Results]The extract had a low-susceptibility inhibitory effect to the three kinds of bacteria,and the minimal inhibitory concentrations(MICs)corresponding to S.aureus,E.coli and B.subtilis were 200,175 and 150 mg/ml,respectively.The minimal bactericidal concentrations(MBCs)corresponding to E.coli and S.aureus were both 500 mg/ml.[Conclusions]This study provides theoretical support for the wide application of H.hedyotidea.展开更多
[Objectives]To study the microscopic characteristics and routine detection of Hedyotis hedyotidea(DC.)Merr.[Methods]The microscopic characteristics of the root,stem cross section and the whole plant powder of H.hedyot...[Objectives]To study the microscopic characteristics and routine detection of Hedyotis hedyotidea(DC.)Merr.[Methods]The microscopic characteristics of the root,stem cross section and the whole plant powder of H.hedyotidea were studied by the methods of slide-shaft section,biological staining and microscopic imaging;the routine detection items such as moisture,ash,acid-insoluble ash and extract were detected and analyzed in accordance with the requirements of Chinese Pharmacopoeia.[Results]The microscopic characteristics of the root,the stem and the whole plant powder of H.hedyotidea were obtained.The moisture content of 10 batches of H.hedyotidea samples was in the range of 4.25%-7.90%,the water-soluble extract is in the range of 15.08%-22.52%,the total ash was in the scope of 8.27%-10.45%,and the acid-insoluble ash was in 0.13%-0.95%.The proposed water content of H.hedyotidea should not exceed 10.00%,the water-soluble extract should not be less than 12.00%,the total ash content should not exceed 13.00%,and the acid-insoluble ash content should not exceed 1.20%.[Conclusions]The results of this experiment can provide a reference for the quality control and quality standard of H.hedyotidea.展开更多
基金Supported by Guangxi University of Chinese Medicine 2019-2021 Guangxi First-class Discipline Construction Open Project(2019XK096).
文摘[Objectives]To establish a high-performance liquid chromatography(HPLC)fingerprint analysis method for Hedyotis hedyotidea,providing a scientific basis for its quality evaluation.[Methods]An Agilent ZORBAX SB-C_(18)column(5μm,4.6 mm×250 mm)was employed with 0.1%phosphoric acid-acetonitrile solution as the mobile phase for gradient elution.The column temperature was maintained at 40℃,detection wavelength at 240 nm,flow rate at 0.8 mL/min,and injection volume at 10μL.The Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(2004A edition)was used to analyze the HPLC fingerprints of 10 batches of H.hedyotidea samples.[Results]An HPLC fingerprint with ursolic acid as the reference standard was established,identifying 17 common characteristic peaks.The similarity of the 10 batches of H.hedyotidea samples ranged from 0.718 to 0.993.[Conclusions]This method demonstrates good systematic performance,with precision(RSD<1.5%),repeatability(RSD<2.0%),and 24-h stability(RSD<2.5%)meeting established requirements.It can provide data support for quality control and standard formulation of H.hedyotidea medicinal materials.
文摘[Objectives]This study was conducted to investigate the antibacterial effect of the extract from Hedyotis hedyotidea(DC)Merr.[Methods]Through in-vitro antibacterial tests,the antibacterial effects of H.hedyotidea ethanol extract on Escherichia coli,Staphylococcus aureus and Bacillus subtilis were analyzed.[Results]The extract had a low-susceptibility inhibitory effect to the three kinds of bacteria,and the minimal inhibitory concentrations(MICs)corresponding to S.aureus,E.coli and B.subtilis were 200,175 and 150 mg/ml,respectively.The minimal bactericidal concentrations(MBCs)corresponding to E.coli and S.aureus were both 500 mg/ml.[Conclusions]This study provides theoretical support for the wide application of H.hedyotidea.
基金Supported by 2019-2021 Guangxi First-class Discipline Construction Open Project of Guangxi University of Chinese Medicine(2019XK096).
文摘[Objectives]To study the microscopic characteristics and routine detection of Hedyotis hedyotidea(DC.)Merr.[Methods]The microscopic characteristics of the root,stem cross section and the whole plant powder of H.hedyotidea were studied by the methods of slide-shaft section,biological staining and microscopic imaging;the routine detection items such as moisture,ash,acid-insoluble ash and extract were detected and analyzed in accordance with the requirements of Chinese Pharmacopoeia.[Results]The microscopic characteristics of the root,the stem and the whole plant powder of H.hedyotidea were obtained.The moisture content of 10 batches of H.hedyotidea samples was in the range of 4.25%-7.90%,the water-soluble extract is in the range of 15.08%-22.52%,the total ash was in the scope of 8.27%-10.45%,and the acid-insoluble ash was in 0.13%-0.95%.The proposed water content of H.hedyotidea should not exceed 10.00%,the water-soluble extract should not be less than 12.00%,the total ash content should not exceed 13.00%,and the acid-insoluble ash content should not exceed 1.20%.[Conclusions]The results of this experiment can provide a reference for the quality control and quality standard of H.hedyotidea.
