[Objective]The study aimed to map QTLs（Quantitative trait loci）of heading stage for phtopeiod-thermo sensitive male sterile line BS366 in wheat.[Method] A population of 234 doubled haploid derived from the cross bet...[Objective]The study aimed to map QTLs（Quantitative trait loci）of heading stage for phtopeiod-thermo sensitive male sterile line BS366 in wheat.[Method] A population of 234 doubled haploid derived from the cross between BS366 and Baiyu149 was planted in Beijing and Funan in 2007,respectively.We used composite interval mapping（CIM）method to analyse the QTLs for heading stage.[Result]We detected 15 QTLs for heading stage.8 QTLs were detected in both Beijing and Funan,which were located on 1B,2A,2D,3B（2 loci）,6B（2 loci）and 7B.A single QTL accounted for 2.42%-10.98% of pheotypic varience.[Conclusion] Eight QTLs which were detected under two environments could be applied for marker-assisted breeding and improvement of BS366.The QTL on chromosome 1B was new for heading stage,which enriches the QTL resource.展开更多
The seed setting rates of total 198 rice cultivars （lines） at heading and flowering stage were investigated under the condition of extreme natural high tem- perature in 2013 so as to analyze the effect of extreme na...The seed setting rates of total 198 rice cultivars （lines） at heading and flowering stage were investigated under the condition of extreme natural high tem- perature in 2013 so as to analyze the effect of extreme natural high temperature on seed setting rate of different rice cultivar （line）. The results showed that the contin- uous high temperature showed certain effects on the seed setting rates of tested materials, and significant differences were shown in seed setting rate among differ- ent rice cultivars （lines）. The seed setting rates differed significantly among indica F1 hybrids derived from different sterile and restorer lines, indicating that the sterile and restorer lines had great effects on heat tolerances of different F~ hybrids. The cor- relation analysis showed that the seed setting rates of conventional indica restorer lines and conventional japonica rice cultivars （lines） were negatively related to the daily highest temperature （P〉0.05）, and the seed setting rates of indica F1 hybrids were positively related to the seed setting rates of their restorer lines. Total four in- dica restorer lines, including Ninghuiguangkangzhan, Shuhui 527, Chenghui 3203 and Xianyin-8, and four new japonica rice cultivars （lines）, including Wuyinjinghui （B2）, Nanjing 4//W3660/Nanjing 44 （B12） and Wuyun 2330/JD6011 （B22） were pre- liminarily screened, and their seed setting rates were all close to the normal level （90%）. The screened rice cultivars （lines） showed higher heat tolerances.展开更多
The newly released super high-yielding hybrid rice combinations, Yueza 122, Fengyou 428, Peiza 67, and the super high-yielding conventional cultivars, Guangchao 3 and Shengtai 1, were grown in both early and late seas...The newly released super high-yielding hybrid rice combinations, Yueza 122, Fengyou 428, Peiza 67, and the super high-yielding conventional cultivars, Guangchao 3 and Shengtai 1, were grown in both early and late seasons. The morphological characters of each population were investigated at the heading stage, and the data were analyzed by using ANOVY and other statistic methods. The plant ideal morphological characters at the heading stage were established as follows： 1 ） for the early-season cropping, 90-105 cm plant height; 11-12 tillers per plant; 35-40 em length and 2.1-2.2 cm width of flag leaf; 46-50 cm length and 1.8-2.1 cm width of the second leaf from the top （L2）; 59-64 cm length and 1.4-1.9 cm width of the third leaf from the top （L3）; 7°-14°, 18° and 200-33° for the ideal leaf angles of the flag leaf, L2 and L3, respectively; 2） for the late-season cropping, 90-100 cm plant height; 9-15 tillers per plant; 30-41 cm length and 1.8-2.0 cm width of flag leaf; 53-61 cm length and 1.3-1.8 cm width of L2; 52-58 cm length and 1.2-1.5 cm width of L3; 9°-19°, 15°-37° and 16°-49° for the ideal leaf angles of the flag leaf, L2 and L3, respectively. The main physiological characteristics of these varieties were also analyzed.展开更多
Heading date is one of the most important agronomic traits of rice,which critically affects rice ecogeographical adaptation,yield and quality.In this study,a late heading date 3(lhd3)mutant was screened from the^(60)...Heading date is one of the most important agronomic traits of rice,which critically affects rice ecogeographical adaptation,yield and quality.In this study,a late heading date 3(lhd3)mutant was screened from the^(60)Co-γirradiation mutant library.The lhd3 delayed heading date in rice under both short day and long day conditions.Map-based cloning combined with Mutmap strategy was adopted to isolate the causal LHD3 gene.The LHD3 gene encodes a DNA_J domain protein,which was ubiquitously expressed in various plant organs,and dominant expressed in stems and leaves.Subcellular localization analysis showed that LHD3 was localized to nucleus,indicating that LHD3 may interact with other elements to regulate the expression of flowering genes.The transcriptions of the heading activators Ehd1,Hd3a and RFT1 significantly decreased in the lhd3 mutant,suggesting that LHD3 may control the heading date through the Ehd1-Hd3a/RFT1 photoperiodic flowering pathway.The variation and haplotype analyses of the genomic region of LHD3 showed that there were 7 haplotypes in the LHD3 region from 4702 accessions.The haplotypes of LHD3 can be divided into two classes:class a and class b,and the heading dates of these two classes were significantly different.Further study showed that two single nucleotide polymorphisms(SNPs),SNP10(G2100C)in Hap II and SNP3(C861T)in Hap VII,may be the functional sites causing early and late heading in accessions.Nucleotide diversity analysis showed LHD3 had been selected in the indica population,rather than in the japonica population.Therefore,the present study sheds light on the regulation of LHD3 on heading date in rice and suggests that LHD3 is a novel promising new target for rice molecular design and breeding improvement.展开更多
基金Supported by the Program of Beijing Basic Research and Innovation Platform for Agricultural Breeding Ⅱ(No.D080705006900801)National"863"Program(No.2009AA101102,2006AA100102)Beijing Natural Science Foundation(No.5091001)~~
文摘[Objective]The study aimed to map QTLs（Quantitative trait loci）of heading stage for phtopeiod-thermo sensitive male sterile line BS366 in wheat.[Method] A population of 234 doubled haploid derived from the cross between BS366 and Baiyu149 was planted in Beijing and Funan in 2007,respectively.We used composite interval mapping（CIM）method to analyse the QTLs for heading stage.[Result]We detected 15 QTLs for heading stage.8 QTLs were detected in both Beijing and Funan,which were located on 1B,2A,2D,3B（2 loci）,6B（2 loci）and 7B.A single QTL accounted for 2.42%-10.98% of pheotypic varience.[Conclusion] Eight QTLs which were detected under two environments could be applied for marker-assisted breeding and improvement of BS366.The QTL on chromosome 1B was new for heading stage,which enriches the QTL resource.
基金Supported by National Natural Science Foundation of China(31201181)Earmarked Fund for China Agriculture Research System(CARS-01-47)Project for Breeding and Demonstration of New Super Rice Varieties~~
文摘The seed setting rates of total 198 rice cultivars （lines） at heading and flowering stage were investigated under the condition of extreme natural high tem- perature in 2013 so as to analyze the effect of extreme natural high temperature on seed setting rate of different rice cultivar （line）. The results showed that the contin- uous high temperature showed certain effects on the seed setting rates of tested materials, and significant differences were shown in seed setting rate among differ- ent rice cultivars （lines）. The seed setting rates differed significantly among indica F1 hybrids derived from different sterile and restorer lines, indicating that the sterile and restorer lines had great effects on heat tolerances of different F~ hybrids. The cor- relation analysis showed that the seed setting rates of conventional indica restorer lines and conventional japonica rice cultivars （lines） were negatively related to the daily highest temperature （P〉0.05）, and the seed setting rates of indica F1 hybrids were positively related to the seed setting rates of their restorer lines. Total four in- dica restorer lines, including Ninghuiguangkangzhan, Shuhui 527, Chenghui 3203 and Xianyin-8, and four new japonica rice cultivars （lines）, including Wuyinjinghui （B2）, Nanjing 4//W3660/Nanjing 44 （B12） and Wuyun 2330/JD6011 （B22） were pre- liminarily screened, and their seed setting rates were all close to the normal level （90%）. The screened rice cultivars （lines） showed higher heat tolerances.
基金supported by China National 863 Program(2001AA211191)100 Innovation Projects of Guangdong Province(2KB64804N)Natural Science Foundation of Guangdong Province(990501).
文摘The newly released super high-yielding hybrid rice combinations, Yueza 122, Fengyou 428, Peiza 67, and the super high-yielding conventional cultivars, Guangchao 3 and Shengtai 1, were grown in both early and late seasons. The morphological characters of each population were investigated at the heading stage, and the data were analyzed by using ANOVY and other statistic methods. The plant ideal morphological characters at the heading stage were established as follows： 1 ） for the early-season cropping, 90-105 cm plant height; 11-12 tillers per plant; 35-40 em length and 2.1-2.2 cm width of flag leaf; 46-50 cm length and 1.8-2.1 cm width of the second leaf from the top （L2）; 59-64 cm length and 1.4-1.9 cm width of the third leaf from the top （L3）; 7°-14°, 18° and 200-33° for the ideal leaf angles of the flag leaf, L2 and L3, respectively; 2） for the late-season cropping, 90-100 cm plant height; 9-15 tillers per plant; 30-41 cm length and 1.8-2.0 cm width of flag leaf; 53-61 cm length and 1.3-1.8 cm width of L2; 52-58 cm length and 1.2-1.5 cm width of L3; 9°-19°, 15°-37° and 16°-49° for the ideal leaf angles of the flag leaf, L2 and L3, respectively. The main physiological characteristics of these varieties were also analyzed.
基金funded by the National Basic Research Program of China(Grant No.2016YFD0100401)National Natural Science Foundation of China(Grant No.31571742)Scientific Research Initiation Fund of Zhejiang University of Science and Technology,China(Grant No.19042142-Y)。
文摘Heading date is one of the most important agronomic traits of rice,which critically affects rice ecogeographical adaptation,yield and quality.In this study,a late heading date 3(lhd3)mutant was screened from the^(60)Co-γirradiation mutant library.The lhd3 delayed heading date in rice under both short day and long day conditions.Map-based cloning combined with Mutmap strategy was adopted to isolate the causal LHD3 gene.The LHD3 gene encodes a DNA_J domain protein,which was ubiquitously expressed in various plant organs,and dominant expressed in stems and leaves.Subcellular localization analysis showed that LHD3 was localized to nucleus,indicating that LHD3 may interact with other elements to regulate the expression of flowering genes.The transcriptions of the heading activators Ehd1,Hd3a and RFT1 significantly decreased in the lhd3 mutant,suggesting that LHD3 may control the heading date through the Ehd1-Hd3a/RFT1 photoperiodic flowering pathway.The variation and haplotype analyses of the genomic region of LHD3 showed that there were 7 haplotypes in the LHD3 region from 4702 accessions.The haplotypes of LHD3 can be divided into two classes:class a and class b,and the heading dates of these two classes were significantly different.Further study showed that two single nucleotide polymorphisms(SNPs),SNP10(G2100C)in Hap II and SNP3(C861T)in Hap VII,may be the functional sites causing early and late heading in accessions.Nucleotide diversity analysis showed LHD3 had been selected in the indica population,rather than in the japonica population.Therefore,the present study sheds light on the regulation of LHD3 on heading date in rice and suggests that LHD3 is a novel promising new target for rice molecular design and breeding improvement.
