The techniques of oxygen electrode polarogra-phy and Fourier transform infrared (FT-IR) spectroscopy were employed to explore the roles of polar head-group of phosphatidylglycerol (PG) molecules in the functional and ...The techniques of oxygen electrode polarogra-phy and Fourier transform infrared (FT-IR) spectroscopy were employed to explore the roles of polar head-group of phosphatidylglycerol (PG) molecules in the functional and structural aspects of photosystem Ⅱ (PS Ⅱ) through enzymatic approach. It was shown that the depletion of PG by treatment of phospholipase C (PLC) on PS Ⅱ particles caused the inhibition of oxygen evolving activity in PS Ⅱ. This effect also gave rise to changes in the protein secondary structures of PS Ⅱ, that is, an increase in a-helical conformation which is compensated by the loss of p-strand structures. It revealed that the head-group of PG molecules plays an important structural role in the maintenance of normal structure of PS Ⅱ proteins, which is required to maintain the appropriate physiological activity of the PS Ⅱ complex such as the oxygen evolving activity. It is suggested that there most probably exist hydrogen-bonding interactions between PG molecules and PS Ⅱ proteins.展开更多
车辆检测是智能交通系统和自动驾驶的重要组成部分。然而,实际交通场景中存在许多不确定因素,导致车辆检测模型的准确率低实时性差。为了解决这个问题,提出了一种快速准确的车辆检测算法——YOLOv8-DEL。使用DGCST(dynamic group convol...车辆检测是智能交通系统和自动驾驶的重要组成部分。然而,实际交通场景中存在许多不确定因素,导致车辆检测模型的准确率低实时性差。为了解决这个问题,提出了一种快速准确的车辆检测算法——YOLOv8-DEL。使用DGCST(dynamic group convolution shuffle transformer)模块代替C2f模块来重构主干网络,以增强特征提取能力并使网络更轻量;添加的P2检测层能使模型更敏锐地定位和检测小目标,同时采用Efficient RepGFPN进行多尺度特征融合,以丰富特征信息并提高模型的特征表达能力;通过结合GroupNorm和共享卷积的优点,设计了一种轻量型共享卷积检测头,在保持精度的前提下,有效减少参数量并提升检测速度。与YOLOv8相比,提出的YOLOv8-DEL在BDD100K数据集和KITTI数据集上,mAP@0.5分别提高了4.8个百分点和1.2个百分点,具有实时检测速度(208.6 FPS和216.4 FPS),在检测精度和速度方面实现了更有利的折中。展开更多
目的超声引导下髋关节囊周围神经(PENG)阻滞联合硬膜外麻醉对股骨头置换术老年患者麻醉效果、术后认知功能及免疫功能的影响。方法选取2021年6月至2024年7月成都市中西医结合医院收治的120例行股骨头置换术的老年患者,采用随机数字表法...目的超声引导下髋关节囊周围神经(PENG)阻滞联合硬膜外麻醉对股骨头置换术老年患者麻醉效果、术后认知功能及免疫功能的影响。方法选取2021年6月至2024年7月成都市中西医结合医院收治的120例行股骨头置换术的老年患者,采用随机数字表法将患者分为对照组(n=60)和研究组(n=60),年龄>60岁,美国麻醉医师协会(ASA)分级Ⅰ或Ⅱ级,研究组接受超声引导下PENG阻滞联合硬膜外麻醉,对照组仅行硬膜外麻醉。比较两组患者的血流动力学指标(心率、平均动脉压)、免疫功能[CD4^(+)、CD4^(+)/CD8^(+)、调节性T细胞(CD4^(+)CD25^(+))]、简易智力状态检查评分(MMSE)、术后认知功能障碍(POCD)发生率、视觉模拟评分法(VAS)。结果研究组麻醉后心率、平均动脉压波动低于对照组(P<0.05);研究组术后6、24、72 h的CD4^(+)、CD4^(+)/CD8^(+)高于对照组,CD4^(+)CD25^(+)低于对照组(P<0.05);研究组术后6 h、术后24 h、术后48 h MMSE评分明显高于对照组(P<0.05);与对照组比较,研究组术后6、24 h POCD发生率明显降低(P<0.05);研究组术后30 min、2 h VAS评分低于对照组(P<0.05);术后24 h两组VAS评分差异无统计学意义(P>0.05)。结论超声引导下PENG阻滞联合硬膜外麻醉效果更佳,镇痛效果好,有利于降低对血流动力学和免疫功能的影响,更快恢复认知,降低POCD发生率。展开更多
基金This work was supported by the National Natural Science Foundation of China (Grant No. 39890390) the State Basic Research Development Plan of China (Grant No. G1998010100)the Innovative Foundation of Laboratory of Photosynthesis Basic Research, Insti
文摘The techniques of oxygen electrode polarogra-phy and Fourier transform infrared (FT-IR) spectroscopy were employed to explore the roles of polar head-group of phosphatidylglycerol (PG) molecules in the functional and structural aspects of photosystem Ⅱ (PS Ⅱ) through enzymatic approach. It was shown that the depletion of PG by treatment of phospholipase C (PLC) on PS Ⅱ particles caused the inhibition of oxygen evolving activity in PS Ⅱ. This effect also gave rise to changes in the protein secondary structures of PS Ⅱ, that is, an increase in a-helical conformation which is compensated by the loss of p-strand structures. It revealed that the head-group of PG molecules plays an important structural role in the maintenance of normal structure of PS Ⅱ proteins, which is required to maintain the appropriate physiological activity of the PS Ⅱ complex such as the oxygen evolving activity. It is suggested that there most probably exist hydrogen-bonding interactions between PG molecules and PS Ⅱ proteins.
文摘目的超声引导下髋关节囊周围神经(PENG)阻滞联合硬膜外麻醉对股骨头置换术老年患者麻醉效果、术后认知功能及免疫功能的影响。方法选取2021年6月至2024年7月成都市中西医结合医院收治的120例行股骨头置换术的老年患者,采用随机数字表法将患者分为对照组(n=60)和研究组(n=60),年龄>60岁,美国麻醉医师协会(ASA)分级Ⅰ或Ⅱ级,研究组接受超声引导下PENG阻滞联合硬膜外麻醉,对照组仅行硬膜外麻醉。比较两组患者的血流动力学指标(心率、平均动脉压)、免疫功能[CD4^(+)、CD4^(+)/CD8^(+)、调节性T细胞(CD4^(+)CD25^(+))]、简易智力状态检查评分(MMSE)、术后认知功能障碍(POCD)发生率、视觉模拟评分法(VAS)。结果研究组麻醉后心率、平均动脉压波动低于对照组(P<0.05);研究组术后6、24、72 h的CD4^(+)、CD4^(+)/CD8^(+)高于对照组,CD4^(+)CD25^(+)低于对照组(P<0.05);研究组术后6 h、术后24 h、术后48 h MMSE评分明显高于对照组(P<0.05);与对照组比较,研究组术后6、24 h POCD发生率明显降低(P<0.05);研究组术后30 min、2 h VAS评分低于对照组(P<0.05);术后24 h两组VAS评分差异无统计学意义(P>0.05)。结论超声引导下PENG阻滞联合硬膜外麻醉效果更佳,镇痛效果好,有利于降低对血流动力学和免疫功能的影响,更快恢复认知,降低POCD发生率。
