Objective: To determine the exposure of wild brown hares [Lepus europaeus(L. europaeus), pallas] to Anaplasma phagocytophilum(A. phagocytophilum), Borrelia burgdorferi(B. burgdorferi) sensu lato, Encephalitozoon cunic...Objective: To determine the exposure of wild brown hares [Lepus europaeus(L. europaeus), pallas] to Anaplasma phagocytophilum(A. phagocytophilum), Borrelia burgdorferi(B. burgdorferi) sensu lato, Encephalitozoon cuniculi(E. cuniculi), Leishmania sp., Neospora caninum(N. caninum) and Toxoplasma gondii(T. gondii). Methods: Two hundred twentytwo blood serum samples of wild brown hares captured in protected areas of the province of Pisa(Central Italy) were tested to detect antibodies against the reported pathogens. Results: Thirty one(14.0%) animals resulted positive for at least one tested agent, with antibody titres ranging from 1:20 to 1:320. In particular, 13(5.8%) samples were positive to B. burgdorferis.l., 11(4.9%) to N. caninum, 3(1.3%) to T. gondii, 2(0.9%) to A. phagocytophilum and 2(0.9%) to Leishmania sp. No samples scored positive to E. cuniculi. Four animals(14.8%) resulted coinfected with 2 different pathogens. Conclusion: The obtained results showed that B. burgdorferi s.l. N. caninum, T. gondii, A. phagocytophilum and Leishmania sp circulate in wild brown hares in Central Italy, suggesting a possible role of L. europaeus as reservoir of these pathogens. The obtained results showed that autochthonous wild brown hares living in Central Italy have been exposed to several pathogens circulating in this area, suggesting a possible role of L. europaeus as reservoir.展开更多
Objective:To investigate clinicopathological,bacteriological and pathological aspects of an experimental infection with Yersinia pseudotuberculosis(Y.pseudotuberculosis)in hares in order to verify the efficacy of sero...Objective:To investigate clinicopathological,bacteriological and pathological aspects of an experimental infection with Yersinia pseudotuberculosis(Y.pseudotuberculosis)in hares in order to verify the efficacy of serology for the in vivo diagnosis.Moreover,the pathogenicity of two Y.pseudotuberculosis strains was investigated in order to detect potential differences.Methods:Twelve European brown hares(Lepus europaeus,Pallas)were experimentally infected per os and via conjunctival mucosae with Y.pseudotuberculosis:six subjects were infected with a strain isolated from a naturally infected hare(YpH)and six subjects with a strain isolated from a naturally infected rabbit(YpR).Two hares were used as negative controls.All animals were subjected to clinical,bacteriological and serological examinations during 9 weeks following the infection and,at the end of the control period,subjects still alive were euthanized and submitted to a complete post mortem examination.Results:All faecal samples collected during the control period were positive for bacteriological examinations and to a PCR for the inv gene of Y.pseudotuberculosis,while only one Yp H-infected hare showed a positive haemocultures.From the 2nd to the 9th week post infection(pi),serological analysis revealed specific antibodies with titers ranging from 1:10 to 1:160 in all YpH-infected and two YpR-infected subjects.All the Yp H-infected and two Yp R-infected hares scored positive for Y.pseudotuberculosis by means of bacteriological investigations.Grossly,suppurative multifocal lesions were detected in liver,spleen,kidney and sub-mandibular lymph nodes in both YpH-and YpR-infected hares and confirmed with histopathology.Pulmonary lesions were observed only in Yp H-infected subjects.Immunohistochemistry confirmed the presence of bacterial antigen in all infected animals.Conclusion:Results of this study revealed that YpH strain is more pathogenic for hares than the YpR strain;moreover the serological test performed in this study could be used for the diagnosis of pseudotuberculosis in hares,whereas post mortem diagnosis should be confirmed by means of bacteriological examination,PCR,histopathology and immunohistochemistry.展开更多
Optimal escape theory predicts that animals would balance the costs and benefits of flight. One cost of not fleeing is the ongoing cost of vigilance for upcoming environmental threats. Our results show that FID increa...Optimal escape theory predicts that animals would balance the costs and benefits of flight. One cost of not fleeing is the ongoing cost of vigilance for upcoming environmental threats. Our results show that FID increases for vigilant hares with predator starting distance, due to the costs acquired by continuing to scan for ecological dangers. The presence of conspecifics within proximity distance for social hare was reduced FID due to collective vigilance, while a solitary animal had greater FID, due to less cooperative defense for predator detection. In both seasons, detection and flight initiation distance of the focal hare increased in open habitat due to a higher probability of detection for upcoming danger, while dense cover provided concealment but reduced the probability of detecting an incoming threat, reducing FID. Moreover, proximity to roads and the nearest refuge significantly influenced anti-predator risk by compensation energy to cope with approaching stimuli. In a landscape with heavy human hunting in retaliation to plantations damage has modified the natural behavior of the hare in the Shigar valley. The findings are discussed in the context of hare FID by humans and the suggestions for management and mitigation of human-wildlife conflict are also considered.展开更多
According to the specimens of ulna-radius of the Cape Hare (Lepus capensie) shot in fall and winter in Shanxi province, China, the ossifications of epiphyses cartilage of ulna-radius are divided into 4 stages. With we...According to the specimens of ulna-radius of the Cape Hare (Lepus capensie) shot in fall and winter in Shanxi province, China, the ossifications of epiphyses cartilage of ulna-radius are divided into 4 stages. With weight of eye lens as a reference, it is proved that the age criterion could be used to distinguish young less than 6–7 months old from those older. This is a pragmatic method to analyze age composition of game-bag in the early period of shooting season when younger holds a large proportion in the hare population. It also is useful in judging the age of hares trapped and estimating the effects of predation on the hare population structure.展开更多
Epidermal growth factor receptor(EGFR)is one of the most important tyrosine kinase receptor families,which plays a pivotal role in cell signaling transduction and physiological processes.Studies on the EGFR gene in hu...Epidermal growth factor receptor(EGFR)is one of the most important tyrosine kinase receptor families,which plays a pivotal role in cell signaling transduction and physiological processes.Studies on the EGFR gene in humans and other species have demonstrated its pivotal role in regulating the sodium ion balance and mediating sodium and water reabsorption in the kidney's proximal tubules.However,the impact of EGFR gene in how the Yarkand hare(Lepus yarkandensis)adapts to extreme environmental habitat remains unclear,The Yarkand hare is a desert-dwelling animal with multiple adaptations to cope with drought.Given the important physiological function of EGFR gene,we strived to understand its role in arid environment and explore the molecular mechanism of drought tolerance in the Yarkand hare.We first performed segmental cloning of the CDS of the Yarkand hare EGFR gene.Then,We constructed the phylogenetic tree of the Yarkand hare's EGFR gene and compared it with that of other species.The results showed that the Yarkand hare was most closely related to the Tolai hare(Lepus tolai).Through quantitative reverse transcription polymerase chain reaction(RT-qPCR),we discovered that EGFR expression in the kidneys of the Yarkand hare was higher than in the allopatric Tolai hare from non-arid areas.Therefore,we hypothesized that EGFR gene overexpression in the kidney of the Yarkand hare may play a crucial role in drought adaptability.Subsequently,we inserted CDS of EGFR gene into a pcDNA3.1-EGFP expression vector to construct recombinant plasmid,which was transfected into HeLa cells and overexpressed.RT-qPCR demonstrated a notable and statistically significant increase in EGFR mRNA expression and western blot proved stable expression of this protein in HeLa cells.Through cell experiments,EGFR gene overexpression markedly enhanced the survival of Hela cells subjected to NaCl,H_(2)O_(2),and heat stresses,increased superoxide dismutase activity,and decreased malondialdehyde content.In conclusion,these findings preliminarily suggest that EGFR might help the Yarkand hare adapt to extreme environmental conditions.EGFR manipulation in vivo could be a promising strategy to enhance the resilience of animals to extreme conditions.展开更多
Soil erosion in the Hare watershed led to significant land degradation,water pollution,and reduced agricultural productivity.Despite its effects,very few researchers have used combined morphometric and RUSLE model tec...Soil erosion in the Hare watershed led to significant land degradation,water pollution,and reduced agricultural productivity.Despite its effects,very few researchers have used combined morphometric and RUSLE model techniques to quantify soil erosion and thereby prioritize impacted areas.This work used an automated GIS-based tool(SWPT)to prioritize crucial areas based on topohydrological and morphometric factors and predict soil loss in sub-watersheds using the RUSLE model.Land use/cover data were obtained from Landsat imagery,while slope and morphometric information were extracted from digital elevation data with a resolution of 12.5 m.Soil erodibility was determined using Ethiopian soil maps,and rainfall erosivity was computed using meteorological data.An average annual soil loss of 49 t ha-1 yr-1 was observed in the Hare watershed.Sub-watershed 11 was found to be the most affected,with an average annual soil loss of 85.12 t ha-1 yr-1and a compound parameter value(CPV)of 0.059.Subwatershed 17 has the least amount of soil loss,with 3.67t ha-1 yr-1 and a CPV of 1.32.The study emphasizes the usefulness of integrating RUSLE and morphometric analysis for soil and water conservation planning,suggesting a variety of modeling tools in data-sparse locations to quantify and prioritize erosion-prone areas.展开更多
The biological characteristics of Lepus timibus in captivity, such as living habit, feeding habit, nutrition composition of main food, growth and development, molting rule, were studied systematically. The results sho...The biological characteristics of Lepus timibus in captivity, such as living habit, feeding habit, nutrition composition of main food, growth and development, molting rule, were studied systematically. The results showed that there were more than 30 kinds of common plants favored by L.timibus. The linear regression equation of weight gain was y=-23.223+30.929 x. The molting sequence in spring successively was head, dorsum, ear,both sides of the chest, abdomen, limbs, tails.展开更多
A novel amide alkaloid,bursatamide A(1),featuring an unprecedented propyl-hexahydronaphthalene carbon frame-work,was isolated from the infrequently studied sea hare Bursatella leachi,alongside a new 3-phenoxypropaneni...A novel amide alkaloid,bursatamide A(1),featuring an unprecedented propyl-hexahydronaphthalene carbon frame-work,was isolated from the infrequently studied sea hare Bursatella leachi,alongside a new 3-phenoxypropanenitrile alkaloid,bursatellin B(2),and twelve known compounds.The structures of 1 and 2 were elucidated through comprehensive spectroscopic data analyses,while their relative and absolute configurations(ACs)were established through total synthesis and a series of quantum chem-ical calculations,including calculated electronic circular dichroism(ECD)spectra,optical rotatory dispersion(ORD)methods,and DP4^(+) probability analyses.Bursatamide A(1)demonstrated inhibitory effects against the human pathogenic bacteria Listeria monocyt-ogenes and Vibrio cholerae.Erythro-bursatellin B(21),a diastereoisomer of 2,exhibited notable antibacterial activity against the fish pathogenic bacterium Streptococcus parauberis FP KSP28,with an MIC90 value of 0.0472 μg·mL^(-1).展开更多
In this study, we report that we successfully cloned and sequenced a chitinase gene from the ovotestis of Kuroda’s sea hare Aplysia kurodai. By using reverse transcription-polymerase chain reaction (RT-PCR) and a sys...In this study, we report that we successfully cloned and sequenced a chitinase gene from the ovotestis of Kuroda’s sea hare Aplysia kurodai. By using reverse transcription-polymerase chain reaction (RT-PCR) and a system for the 5’ and 3’ rapid amplification of cDNA ends, we obtained a 1352 bp chitinase gene (AkChi) from the ovotestis of A. kurodai. AkChi contains a 1263 bp open reading frame that encodes 421 amino acids. The domain structure predicted from the deduced amino acid sequence was an N-terminal signal peptide and a catalytic domain of glycoside hydrolase (GH) family 18 chitinase. A comparative analysis of the deduced amino acid sequences of AkChi with those of the acidic mammalian chitinase of the California sea hare Aplysia californica revealed the highest homology at 83%. The purified chitinase from the ovotestis was digested by trypsin, and 119 residues of digested peptides were consistent with the deduced amino acid sequence of AkChi. We used RT-PCR to evaluate the expression of AkChi in various tissues of A. kurodai, and we observed that AkChi was expressed only in the ovotestis. A phylogenetic tree analysis, performed using the amino acid sequences of AkChi and known GH family 18 chitinases, showed that AkChi was separated from the molluscan chitinases with a chitin binding domain. To our knowledge, this is the first study demonstrating the cDNA cloning of an ovotestis chitinase from a sea hare.展开更多
The paper proposes to investigate feelings of guilt, love, and forgiveness as Hare＇s The Reader （2009）. It will focus on Hare＇s selection of detail in his they manifest themselves in David screenplay The Reader; a...The paper proposes to investigate feelings of guilt, love, and forgiveness as Hare＇s The Reader （2009）. It will focus on Hare＇s selection of detail in his they manifest themselves in David screenplay The Reader; a dramatic adaptation of Bernhard Schlink＇s novel of 1995. This will involve comparing the novel to the dramatic text for the purpose of showing the movement from narrative to dramatic rendering. The paper aspires to reach the conclusion that Hare＇s screenplay, The Reader creates a world of signification where the interplay of guilt, love, and forgiveness shape the textual and stage space. Read in light of Shoshana Felman＇s The Juridical Unconscious （2002）, the play investigates the traumatic history of Nazi Germany with scope for analysis of guilt and forgiveness and the possibility of atonement through love and literature. The focus of the paper will be on the ideological and formal structure of the play and its impact on meanings and interpretations.展开更多
We applied <em>n</em>-variable conserving nonlinear differential equations (<em>n</em>-CNDEs) to the population data of the 10-year cycles of Canadian lynx (1821-2016) and the snowshoe hare (18...We applied <em>n</em>-variable conserving nonlinear differential equations (<em>n</em>-CNDEs) to the population data of the 10-year cycles of Canadian lynx (1821-2016) and the snowshoe hare (1845-1921). Modeling external effects as perturbations to population dynamics, recovering and restorations from disintegrations (or extinctions), stability and survival strategies are discussed in terms of the conservation law inherent to dynamical interactions among species. The 2-variable conserving nonlinear interaction (2CNIs) is extended to 3, 4, ... <em>n</em>-variable conserving nonlinear interactions (<em>n</em>-CNIs) of species by adjusting minimum unknown parameters. The population cycle of species is a manifestation of conservation laws existing in complicated ecosystems, which is suggested from the CNDE analysis as <em>a standard rhythm</em> of interactions. The ecosystem is a consequence of the long history of nonlinear interactions and evolutions among life-beings and the natural environment, and the population dynamics of an ecosystem are observed as approximate CNIs. Physical analyses of the conserving quantity in nonlinear interactions would help us understand why and how they have developed. The standard rhythm found in nonlinear interactions should be considered as a manifestation of the survival strategy and the survival of the fittest to the balance of biological systems. The CNDEs and nonlinear differential equations with time-dependent coefficients would help find useful physical information on the survival of the fittest and symbiosis in an ecosystem.展开更多
In order to analyze effects of domestication manner and age on reproduction of Lepus timidus,contrast test using large-cage and small-cage domestication manners and reproduction test with different ages of L.timidus w...In order to analyze effects of domestication manner and age on reproduction of Lepus timidus,contrast test using large-cage and small-cage domestication manners and reproduction test with different ages of L.timidus was performed.The results showed that there was no significant difference in reproduction and production performance of L.timidus between small-cage and large-cage domestication manners（P〈0.05）.The reproduction performance of female middle-aged rabbit was superior to that of female youth rabbit.展开更多
This essay is intended to present the resemblances and differences between Yeats'poem Collarbone of a Hare and Blake's poem Garden of Love in regard to their theme and poetical techniques.
文摘Objective: To determine the exposure of wild brown hares [Lepus europaeus(L. europaeus), pallas] to Anaplasma phagocytophilum(A. phagocytophilum), Borrelia burgdorferi(B. burgdorferi) sensu lato, Encephalitozoon cuniculi(E. cuniculi), Leishmania sp., Neospora caninum(N. caninum) and Toxoplasma gondii(T. gondii). Methods: Two hundred twentytwo blood serum samples of wild brown hares captured in protected areas of the province of Pisa(Central Italy) were tested to detect antibodies against the reported pathogens. Results: Thirty one(14.0%) animals resulted positive for at least one tested agent, with antibody titres ranging from 1:20 to 1:320. In particular, 13(5.8%) samples were positive to B. burgdorferis.l., 11(4.9%) to N. caninum, 3(1.3%) to T. gondii, 2(0.9%) to A. phagocytophilum and 2(0.9%) to Leishmania sp. No samples scored positive to E. cuniculi. Four animals(14.8%) resulted coinfected with 2 different pathogens. Conclusion: The obtained results showed that B. burgdorferi s.l. N. caninum, T. gondii, A. phagocytophilum and Leishmania sp circulate in wild brown hares in Central Italy, suggesting a possible role of L. europaeus as reservoir of these pathogens. The obtained results showed that autochthonous wild brown hares living in Central Italy have been exposed to several pathogens circulating in this area, suggesting a possible role of L. europaeus as reservoir.
文摘Objective:To investigate clinicopathological,bacteriological and pathological aspects of an experimental infection with Yersinia pseudotuberculosis(Y.pseudotuberculosis)in hares in order to verify the efficacy of serology for the in vivo diagnosis.Moreover,the pathogenicity of two Y.pseudotuberculosis strains was investigated in order to detect potential differences.Methods:Twelve European brown hares(Lepus europaeus,Pallas)were experimentally infected per os and via conjunctival mucosae with Y.pseudotuberculosis:six subjects were infected with a strain isolated from a naturally infected hare(YpH)and six subjects with a strain isolated from a naturally infected rabbit(YpR).Two hares were used as negative controls.All animals were subjected to clinical,bacteriological and serological examinations during 9 weeks following the infection and,at the end of the control period,subjects still alive were euthanized and submitted to a complete post mortem examination.Results:All faecal samples collected during the control period were positive for bacteriological examinations and to a PCR for the inv gene of Y.pseudotuberculosis,while only one Yp H-infected hare showed a positive haemocultures.From the 2nd to the 9th week post infection(pi),serological analysis revealed specific antibodies with titers ranging from 1:10 to 1:160 in all YpH-infected and two YpR-infected subjects.All the Yp H-infected and two Yp R-infected hares scored positive for Y.pseudotuberculosis by means of bacteriological investigations.Grossly,suppurative multifocal lesions were detected in liver,spleen,kidney and sub-mandibular lymph nodes in both YpH-and YpR-infected hares and confirmed with histopathology.Pulmonary lesions were observed only in Yp H-infected subjects.Immunohistochemistry confirmed the presence of bacterial antigen in all infected animals.Conclusion:Results of this study revealed that YpH strain is more pathogenic for hares than the YpR strain;moreover the serological test performed in this study could be used for the diagnosis of pseudotuberculosis in hares,whereas post mortem diagnosis should be confirmed by means of bacteriological examination,PCR,histopathology and immunohistochemistry.
文摘Optimal escape theory predicts that animals would balance the costs and benefits of flight. One cost of not fleeing is the ongoing cost of vigilance for upcoming environmental threats. Our results show that FID increases for vigilant hares with predator starting distance, due to the costs acquired by continuing to scan for ecological dangers. The presence of conspecifics within proximity distance for social hare was reduced FID due to collective vigilance, while a solitary animal had greater FID, due to less cooperative defense for predator detection. In both seasons, detection and flight initiation distance of the focal hare increased in open habitat due to a higher probability of detection for upcoming danger, while dense cover provided concealment but reduced the probability of detecting an incoming threat, reducing FID. Moreover, proximity to roads and the nearest refuge significantly influenced anti-predator risk by compensation energy to cope with approaching stimuli. In a landscape with heavy human hunting in retaliation to plantations damage has modified the natural behavior of the hare in the Shigar valley. The findings are discussed in the context of hare FID by humans and the suggestions for management and mitigation of human-wildlife conflict are also considered.
文摘According to the specimens of ulna-radius of the Cape Hare (Lepus capensie) shot in fall and winter in Shanxi province, China, the ossifications of epiphyses cartilage of ulna-radius are divided into 4 stages. With weight of eye lens as a reference, it is proved that the age criterion could be used to distinguish young less than 6–7 months old from those older. This is a pragmatic method to analyze age composition of game-bag in the early period of shooting season when younger holds a large proportion in the hare population. It also is useful in judging the age of hares trapped and estimating the effects of predation on the hare population structure.
基金supported by the Central Guidance for Local Projects of Xinjiang(ZYYD2024ZY04)the National Natural Science Foundation of China(32260116).
文摘Epidermal growth factor receptor(EGFR)is one of the most important tyrosine kinase receptor families,which plays a pivotal role in cell signaling transduction and physiological processes.Studies on the EGFR gene in humans and other species have demonstrated its pivotal role in regulating the sodium ion balance and mediating sodium and water reabsorption in the kidney's proximal tubules.However,the impact of EGFR gene in how the Yarkand hare(Lepus yarkandensis)adapts to extreme environmental habitat remains unclear,The Yarkand hare is a desert-dwelling animal with multiple adaptations to cope with drought.Given the important physiological function of EGFR gene,we strived to understand its role in arid environment and explore the molecular mechanism of drought tolerance in the Yarkand hare.We first performed segmental cloning of the CDS of the Yarkand hare EGFR gene.Then,We constructed the phylogenetic tree of the Yarkand hare's EGFR gene and compared it with that of other species.The results showed that the Yarkand hare was most closely related to the Tolai hare(Lepus tolai).Through quantitative reverse transcription polymerase chain reaction(RT-qPCR),we discovered that EGFR expression in the kidneys of the Yarkand hare was higher than in the allopatric Tolai hare from non-arid areas.Therefore,we hypothesized that EGFR gene overexpression in the kidney of the Yarkand hare may play a crucial role in drought adaptability.Subsequently,we inserted CDS of EGFR gene into a pcDNA3.1-EGFP expression vector to construct recombinant plasmid,which was transfected into HeLa cells and overexpressed.RT-qPCR demonstrated a notable and statistically significant increase in EGFR mRNA expression and western blot proved stable expression of this protein in HeLa cells.Through cell experiments,EGFR gene overexpression markedly enhanced the survival of Hela cells subjected to NaCl,H_(2)O_(2),and heat stresses,increased superoxide dismutase activity,and decreased malondialdehyde content.In conclusion,these findings preliminarily suggest that EGFR might help the Yarkand hare adapt to extreme environmental conditions.EGFR manipulation in vivo could be a promising strategy to enhance the resilience of animals to extreme conditions.
文摘Soil erosion in the Hare watershed led to significant land degradation,water pollution,and reduced agricultural productivity.Despite its effects,very few researchers have used combined morphometric and RUSLE model techniques to quantify soil erosion and thereby prioritize impacted areas.This work used an automated GIS-based tool(SWPT)to prioritize crucial areas based on topohydrological and morphometric factors and predict soil loss in sub-watersheds using the RUSLE model.Land use/cover data were obtained from Landsat imagery,while slope and morphometric information were extracted from digital elevation data with a resolution of 12.5 m.Soil erodibility was determined using Ethiopian soil maps,and rainfall erosivity was computed using meteorological data.An average annual soil loss of 49 t ha-1 yr-1 was observed in the Hare watershed.Sub-watershed 11 was found to be the most affected,with an average annual soil loss of 85.12 t ha-1 yr-1and a compound parameter value(CPV)of 0.059.Subwatershed 17 has the least amount of soil loss,with 3.67t ha-1 yr-1 and a CPV of 1.32.The study emphasizes the usefulness of integrating RUSLE and morphometric analysis for soil and water conservation planning,suggesting a variety of modeling tools in data-sparse locations to quantify and prioritize erosion-prone areas.
基金Supported by National Natural Science Foundation of China(30870109)
文摘The biological characteristics of Lepus timibus in captivity, such as living habit, feeding habit, nutrition composition of main food, growth and development, molting rule, were studied systematically. The results showed that there were more than 30 kinds of common plants favored by L.timibus. The linear regression equation of weight gain was y=-23.223+30.929 x. The molting sequence in spring successively was head, dorsum, ear,both sides of the chest, abdomen, limbs, tails.
基金supported by the National Key Research andDevelopment ProgramofChina(Nos.2021YFF0502400,2022YFC2804100)the Natural Science Foundation of China(Nos.82022069,42076099)+4 种基金Shandong Laboratory Program(No.SYS202205)the“Youth Innovation Promotion Association”of CAS(No.Y202065)the Fundamental Research Projects of Science&Technology In-novation and development Plan in Yantai City(No.2023JCYJ057)the CAS Youth Interdisciplinary Team,the Shandong Science and Technology Major Project of Innovation and Entrepreneurship Community with Antibodydrug(No.E321020)Youth Fund from Natural Science Foundation of Shandong Province(No.ZR2023QD162)。
文摘A novel amide alkaloid,bursatamide A(1),featuring an unprecedented propyl-hexahydronaphthalene carbon frame-work,was isolated from the infrequently studied sea hare Bursatella leachi,alongside a new 3-phenoxypropanenitrile alkaloid,bursatellin B(2),and twelve known compounds.The structures of 1 and 2 were elucidated through comprehensive spectroscopic data analyses,while their relative and absolute configurations(ACs)were established through total synthesis and a series of quantum chem-ical calculations,including calculated electronic circular dichroism(ECD)spectra,optical rotatory dispersion(ORD)methods,and DP4^(+) probability analyses.Bursatamide A(1)demonstrated inhibitory effects against the human pathogenic bacteria Listeria monocyt-ogenes and Vibrio cholerae.Erythro-bursatellin B(21),a diastereoisomer of 2,exhibited notable antibacterial activity against the fish pathogenic bacterium Streptococcus parauberis FP KSP28,with an MIC90 value of 0.0472 μg·mL^(-1).
文摘In this study, we report that we successfully cloned and sequenced a chitinase gene from the ovotestis of Kuroda’s sea hare Aplysia kurodai. By using reverse transcription-polymerase chain reaction (RT-PCR) and a system for the 5’ and 3’ rapid amplification of cDNA ends, we obtained a 1352 bp chitinase gene (AkChi) from the ovotestis of A. kurodai. AkChi contains a 1263 bp open reading frame that encodes 421 amino acids. The domain structure predicted from the deduced amino acid sequence was an N-terminal signal peptide and a catalytic domain of glycoside hydrolase (GH) family 18 chitinase. A comparative analysis of the deduced amino acid sequences of AkChi with those of the acidic mammalian chitinase of the California sea hare Aplysia californica revealed the highest homology at 83%. The purified chitinase from the ovotestis was digested by trypsin, and 119 residues of digested peptides were consistent with the deduced amino acid sequence of AkChi. We used RT-PCR to evaluate the expression of AkChi in various tissues of A. kurodai, and we observed that AkChi was expressed only in the ovotestis. A phylogenetic tree analysis, performed using the amino acid sequences of AkChi and known GH family 18 chitinases, showed that AkChi was separated from the molluscan chitinases with a chitin binding domain. To our knowledge, this is the first study demonstrating the cDNA cloning of an ovotestis chitinase from a sea hare.
文摘The paper proposes to investigate feelings of guilt, love, and forgiveness as Hare＇s The Reader （2009）. It will focus on Hare＇s selection of detail in his they manifest themselves in David screenplay The Reader; a dramatic adaptation of Bernhard Schlink＇s novel of 1995. This will involve comparing the novel to the dramatic text for the purpose of showing the movement from narrative to dramatic rendering. The paper aspires to reach the conclusion that Hare＇s screenplay, The Reader creates a world of signification where the interplay of guilt, love, and forgiveness shape the textual and stage space. Read in light of Shoshana Felman＇s The Juridical Unconscious （2002）, the play investigates the traumatic history of Nazi Germany with scope for analysis of guilt and forgiveness and the possibility of atonement through love and literature. The focus of the paper will be on the ideological and formal structure of the play and its impact on meanings and interpretations.
文摘We applied <em>n</em>-variable conserving nonlinear differential equations (<em>n</em>-CNDEs) to the population data of the 10-year cycles of Canadian lynx (1821-2016) and the snowshoe hare (1845-1921). Modeling external effects as perturbations to population dynamics, recovering and restorations from disintegrations (or extinctions), stability and survival strategies are discussed in terms of the conservation law inherent to dynamical interactions among species. The 2-variable conserving nonlinear interaction (2CNIs) is extended to 3, 4, ... <em>n</em>-variable conserving nonlinear interactions (<em>n</em>-CNIs) of species by adjusting minimum unknown parameters. The population cycle of species is a manifestation of conservation laws existing in complicated ecosystems, which is suggested from the CNDE analysis as <em>a standard rhythm</em> of interactions. The ecosystem is a consequence of the long history of nonlinear interactions and evolutions among life-beings and the natural environment, and the population dynamics of an ecosystem are observed as approximate CNIs. Physical analyses of the conserving quantity in nonlinear interactions would help us understand why and how they have developed. The standard rhythm found in nonlinear interactions should be considered as a manifestation of the survival strategy and the survival of the fittest to the balance of biological systems. The CNDEs and nonlinear differential equations with time-dependent coefficients would help find useful physical information on the survival of the fittest and symbiosis in an ecosystem.
文摘In order to analyze effects of domestication manner and age on reproduction of Lepus timidus,contrast test using large-cage and small-cage domestication manners and reproduction test with different ages of L.timidus was performed.The results showed that there was no significant difference in reproduction and production performance of L.timidus between small-cage and large-cage domestication manners（P〈0.05）.The reproduction performance of female middle-aged rabbit was superior to that of female youth rabbit.
文摘This essay is intended to present the resemblances and differences between Yeats'poem Collarbone of a Hare and Blake's poem Garden of Love in regard to their theme and poetical techniques.
