Nano-crystalline diamond （NCD） films were prepared on poly-crystalline diamond （PCD） thick flims by the microwave plasma enhanced chemical vapor deposition （MPCVD） method. Free standing PCD thick film （50 mm in...Nano-crystalline diamond （NCD） films were prepared on poly-crystalline diamond （PCD） thick flims by the microwave plasma enhanced chemical vapor deposition （MPCVD） method. Free standing PCD thick film （50 mm in diameter） with a thickness of 413 μm was deposited in CHn/H2 plasma. It was then abraded for 2 hours and finally cut into pieces in a size of 10×10 mm^2 by pulse laser. NCD fihns were deposited on the thick film substrates by introducing a micro-crystalline diamond （MCD） interlayer. Results showed that a higher carbon concentration （5%） and a lower substrate temperature （650℃） were feasible to obtain a highly smooth interlayer, and the appropriate addition of oxygen （2%） into the gas mixture was conducive to obtaining a smooth nano-crystalline diamond film with a tiny grain size.展开更多
[Objectives]To develop an HPLC fingerprint analysis method for the medicinal material of Lysimachia foenum-graecum Hance,thereby providing a foundation for its quality control.[Methods]Samples of L.foenum-graecum coll...[Objectives]To develop an HPLC fingerprint analysis method for the medicinal material of Lysimachia foenum-graecum Hance,thereby providing a foundation for its quality control.[Methods]Samples of L.foenum-graecum collected from 10 distinct locations in Guangxi were analyzed using HPLC,and chromatographic fingerprints were established.The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Edition)was employed for common peak calibration and similarity evaluation.Additionally,principal component analysis was performed on the common peak area data.[Results]An HPLC fingerprint of L.foenum-graecum was developed,identifying a total of 13 common peaks.Among these,four characteristic components were specifically identified:chlorogenic acid,myricetin,quercetin,and kaempferol.The kaempferol chromatographic peak,exhibiting good resolution and a stable peak shape,was selected as the reference peak.The similarity indices between the fingerprints of the 10 sample batches and the reference fingerprint ranged from 0.954 to 0.995,indicating a relatively high consistency in the chemical composition of L.foenum-graecum from different origins.Principal component analysis identified two principal components,which together accounted for 89.45%of the cumulative variance,effectively capturing the primary chemical differences among the samples.[Conclusions]The established HPLC fingerprint method is straightforward to implement,stable,reliable,and exhibits high specificity.When combined with similarity evaluation and principal component analysis,it offers a scientific basis for developing quality standards for L.foenum-graecum medicinal materials.展开更多
[Objectives]To evaluate the in vitro antibacterial,antioxidant,andα-glucosidase inhibitory activities of the ethanol total extract and four different polarity fractions(n-butanol,ethyl acetate,petroleum ether,and wat...[Objectives]To evaluate the in vitro antibacterial,antioxidant,andα-glucosidase inhibitory activities of the ethanol total extract and four different polarity fractions(n-butanol,ethyl acetate,petroleum ether,and water)of Pilea peltata Hance,so as to provide a reference for its further development and research.[Methods]The antibacterial activity of P.peltata was evaluated in vitro by determining the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of its ethanol total extract and four different polarity fractions against seven test bacterial strains using the broth microdilution method.The in vitro antioxidant activity was investigated through DPPH radical,hydroxyl radical,and superoxide anion radical scavenging assays,with vitamin C(Vit C)as the positive control and the half maximal scavenging concentration(IC 50)as the evaluation indicator.The in vitroα-glucosidase inhibitory activity was assessed by measuring the peak area of p-nitrophenol(PNP),the hydrolysis product of 4-nitrophenylα-D-glucopyranoside(PNPG),via high-performance liquid chromatography(HPLC),using the half maximal inhibitory concentration(IC_(50))as the evaluation indicator.[Results]Both the ethanol total extract and the four different polarity fractions of P.peltata exhibited significant in vitro anti-Streptococcus pneumoniae activity.The DPPH radical scavenging capacities of the ethanol total extract and the various fractions were all weaker than that of VitC,with the order of efficacy being:n-butanol fraction>ethanol total extract>ethyl acetate fraction>petroleum ether fraction>aqueous fraction.For hydroxyl radical scavenging activity,the efficacy order of P.peltata fractions was:n-butanol extract>ethyl acetate extract>ethanol total extract>petroleum ether extract>aqueous extract.Notably,the n-butanol fraction(IC_(50)=0.068±0.001)demonstrated stronger activity than VitC(IC_(50)=0.097±0.001).The activity of the ethyl acetate fraction(IC_(50)=0.096±0.004)was comparable to that of VitC(IC_(50)=0.097±0.001).The superoxide anion scavenging capacities of the ethanol total extract and different polarity fractions from P.peltata were all weaker than that of VitC,with the order of efficacy being:n-butanol fraction>ethyl acetate fraction>ethanol total extract>petroleum ether fraction>aqueous fraction.The ethanol total extract and aqueous fraction of Pilea peltata showed no significant in vitroα-glucosidase inhibitory activity.Compared with the acarbose group,the IC 50 values of the ethyl acetate fraction and the n-butanol fraction both showed highly significant differences(P<0.01).[Conclusions]This study provides an experimental basis for the pharmacodynamic study and active component study of P.peltata.展开更多
Abstract： To investigate the chemical constituents of the roots of Polygala wattersii Hance, the separation and purification were performed by solvent extraction and repeated column chromatography （CC） on silica ge...Abstract： To investigate the chemical constituents of the roots of Polygala wattersii Hance, the separation and purification were performed by solvent extraction and repeated column chromatography （CC） on silica gel, Sephadex LH-20 and macroporous resin D101, preparative TLC and semi-preparative HPLC. The structures were identified by spectroscopic analysis and comparison of their 1H and 13C NMR data with those reported in literatures. Twenty-three known compounds, including eleven xanthones （1-11）, nine sugar esters （12-20）, two triterpenoid saponins （21 and 22） and one phenylpropanoid （23） were isolated and their structures were identified as 1,3-dihydroxyxanthone （1）, 1-hydroxy-3-methoxyxanthone （2）, 1,3-dihydroxy-2-methoxyxanthone （3）, 1,3,7-trihydroxy-2- methoxyxanthone （4）, 1,3,6-trihydroxy-2,7-dimethoxyxanthone （5）, 1,6,7-trihydroxy-2,3-dimethoxyxanthone （6）, 1,7-dihydroxy- 2,3-methylenedioxyxanthone （7）, 1,7-dimethoxyxanthone （8）, 1,2,3-trimethoxyxanthone （9）, 1-methoxy-2,3-methylenedioxyxanthone （10）, 6-hydroxy-0-methoxy-2,3-methylenedioxyxanthone （11）, 3＇-O-feruloyl-6-O-acetyl sucrose （12）, arillatose B （13）, sibricose A5 （14）, sibricose A6 （15）, 3＇,6-di-O-sinapoyl sucrose （16）, tenufoliside A （17）, 3＇-O-3,4,5-trimethoxycinnamoyl-6-O-p-methoxybenzoyl sucrose （18）, glomeratose A （19）, 1-O-p-coumaroyl-D-glucopyranose （20）, bayogenin-3-O-glucoside （21）, tenufolin （22）, and sinapic acid （23）. Among them, compounds 2 and 12 were obtained from genus Polygala for the first time, and except compound 16, all others were isolated from this species for the first time.展开更多
[Objective] To construct prokaryotic expression vectors encoding gene Erb3binding protein （EBP1）, which plays important roles in regulating plant organ size from Nervilia fordii （Hance） Schltr. [Methods] PCR produ...[Objective] To construct prokaryotic expression vectors encoding gene Erb3binding protein （EBP1）, which plays important roles in regulating plant organ size from Nervilia fordii （Hance） Schltr. [Methods] PCR products of NfEBP1 with particular restriction sites and expression vectors, pET-28 and pET-16b were digested. Ligation, transformation and selection were performed to construct the recombinant plasmids pET-28-NfEBP1 and pET-16-NfEBP1. The recombinant plasmids were transformed into E. coli BL21 using heat -shock transformation. [Results] Recombinant plasmids pET-28-NfEBP1-1188 and pET-16-NfEBP1-1188 were constructed and transformed into expressional host cells, E. coli BL21, and validated by colony PCR, sequencing and double digestion. [Conclusion] Prokaryotic expression vectors of EBP1 gene from N. fordii were successfully constructed, which laid the foundation for characterization of the gene function.展开更多
In the present study, we optimized the procedures to pretreat the parental material, to collect, sterilize and inoculate the explants in the tissue culture of Ar- disia mamillata Hance, so that more effective sterile ...In the present study, we optimized the procedures to pretreat the parental material, to collect, sterilize and inoculate the explants in the tissue culture of Ar- disia mamillata Hance, so that more effective sterile explants can be obtained. The results revealed the optimal procedures. In detail, the parental materials were trans- ferred and pretreated in laboratory in February. The stem tips of new branches were collected in middle March, cleaned with eradicator and then rinsed with tap water for 1-2 h. After that, the explants were surface sterilized with 70% alcohol for 30 s, rinsed with sterile water 2-3 times, sterilized with 0.1% mercuric chloride for 4 min, rinsed with sterile water three times and sterilized with 0.1% mercuric chlo- ride for 4 min again. Finally, they were inoculated into medium after being rinsed with sterile water 5-8 times. By this method, the pollution rate of explants can be greatly reduced while their survival rate can be significantly improved.展开更多
Phytoremediation is a cost-effective and environment-friendly strategy for decontaminating heavy-metal-contaminated soil.However, the practical use of phytoremediation is constrained by the low biomass of plants and l...Phytoremediation is a cost-effective and environment-friendly strategy for decontaminating heavy-metal-contaminated soil.However, the practical use of phytoremediation is constrained by the low biomass of plants and low bioavailability of heavy metals in soil.A pot experiment was conducted to investigate the effects of the metal chelator ethylenediaminetetraacetic acid(EDTA) and EDTA in combination with plant growth-promoting rhizobacteria(Burkholderia sp.D54 or Burkholderia sp.D416) on the growth and metal uptake of the hyperaccumulator Sedum alfredii Hance.According to the results, EDTA application decreased shoot and root biomass by 50% and 43%, respectively.The soil respiration and Cd,Pb, Zn uptake were depressed, while the photosynthetic rate, glutathione and phytochelatin(PC) contents were increased by EDTA application.Interestingly, Burkholderia sp.D54 and Burkholderia sp.D416 inoculation significantly relieved the inhibitory effects of EDTA on plant growth and soil respiration.Compared with the control, EDTA + D416 treatment increased the Cd concentration in shoots and decreased the Pb concentration in shoots and roots, but did not change the Zn concentration in S.alfredii plants.Furthermore,EDTA, EDTA + D54 and EDTA + D416 application increased the cysteine and PC contents in S.alfredii(p < 0.05);among all tested PCs, the most abundant species was PC2, and compared with the control, the PC2 content was increased by 371.0%, 1158.6% and 815.6%,respectively.These results will provide some insights into the practical use of EDTA and PGPR in the phytoremediation of heavy-metal-contaminated soil by S.alfredii.展开更多
Zn accumulation and subcellular distribution in leaves of the hyperaccumulating ecotype （HE） and non-hyperaccumulating ecotype （NHE） of Sedum alfredii Hance were studied using radiotracer and gradient centrifugati...Zn accumulation and subcellular distribution in leaves of the hyperaccumulating ecotype （HE） and non-hyperaccumulating ecotype （NHE） of Sedum alfredii Hance were studied using radiotracer and gradient centrifugation techniques. Leaf Zn accumulation in the HE of S. alfredii was 18.5-26.7 times greater than that in the NHE when the plants were grown at 1-500μmol Zn L-1. Leaf section uptake of 65Zn was highly dependent on external Zn levels. Greater 65Zn uptake in HE was noted only at external Zn levels 〉 100μmol L-1. Zinc subcellular distribution in the leaves of the two ecotypes of S. alfredii was： cell wall 〉 soluble fraction 〉 cell organelle. However, more Zn was distributed to the leaf cell wall and soluble fractions for HE than for NHE. In the leaf of HE, 91%-94% of the Zn was found in the cell walls and the soluble fraction and only 6%-9% Zn was distributed in the cell organelle fraction. For NHE, about 20%-26% Zn was recovered in the cell organelle fraction. In stems, Zn distribution to the ceil wail fraction was approximately two fold greater in the HE than that in the NHE. For the hyperaccumulating ecotype of S. alfredii, the cell wall and the vacuole played a very important role in Zn tolerance and hyperaccumulation.展开更多
Objective:To study the effect of Alpinia officinarum Hance(A.officinarum) 80% alcohol extract on the primary dysmenorrhea.Methods:A.officinarum 80% alcohol extract were enriched by macroporous adsorption resins.Female...Objective:To study the effect of Alpinia officinarum Hance(A.officinarum) 80% alcohol extract on the primary dysmenorrhea.Methods:A.officinarum 80% alcohol extract were enriched by macroporous adsorption resins.Female mice of primary dysmenorrhea model were established by oxytocin induction; the effects of A.officinarum 80% alcohol extract on primary dysmenorrhea were observed by body twist method; and the homogenate level of prostaglandin F_(2α)(PGF_(2α)),prostaglandin E_2(PGE_2) and Ca^(2+) in the uterus were observed in oxytocin-induced female mice.Results:The writhing frequency of primary dysmenorrhea mice was significantly decreased after treatment of A.officinarum 80% alcohol extract and the level of PGF_(2α),PGE_2 and Ca^(2+) in mice uterus was significantly decreased(P<0.05,P<0.01) in groups of mice treated with middle and high dosage of A.officinarum 80% alcohol extract compared with that of model group.Conclusions:These findings suggest that A.Officinarum 80% alcohol extract can significantly relieve primary dysmenorrhea.展开更多
Two new phloroglucinol glycosides, lysidiciside A (1) and lysidiciside B (2), were isolated from the roots of Lysidice rhodostega Hance. Their structures were determined as 1-[(3-methylbutyryl)phloroglucinol]-β-D- g...Two new phloroglucinol glycosides, lysidiciside A (1) and lysidiciside B (2), were isolated from the roots of Lysidice rhodostega Hance. Their structures were determined as 1-[(3-methylbutyryl)phloroglucinol]-β-D- glucopyranoside (1), 1- [(3-methylbutyryl)phloro- glucinol]-β-D-glucopyranosyl-5-O-β-D-glucopyranoside (2) on the basis of chemical and spectral analysis .展开更多
基金supported by the Research Pund of Hubei Provincial Department of Education of China (No.Q20081505)
文摘Nano-crystalline diamond （NCD） films were prepared on poly-crystalline diamond （PCD） thick flims by the microwave plasma enhanced chemical vapor deposition （MPCVD） method. Free standing PCD thick film （50 mm in diameter） with a thickness of 413 μm was deposited in CHn/H2 plasma. It was then abraded for 2 hours and finally cut into pieces in a size of 10×10 mm^2 by pulse laser. NCD fihns were deposited on the thick film substrates by introducing a micro-crystalline diamond （MCD） interlayer. Results showed that a higher carbon concentration （5%） and a lower substrate temperature （650℃） were feasible to obtain a highly smooth interlayer, and the appropriate addition of oxygen （2%） into the gas mixture was conducive to obtaining a smooth nano-crystalline diamond film with a tiny grain size.
基金Supported by 2023 Self-funded Research Project of the Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine(GXZYL20230369)2022 Internal Talent Research Project of Affiliated Hospital of Youjiang Medical University for Nationalities(Y202210319).
文摘[Objectives]To develop an HPLC fingerprint analysis method for the medicinal material of Lysimachia foenum-graecum Hance,thereby providing a foundation for its quality control.[Methods]Samples of L.foenum-graecum collected from 10 distinct locations in Guangxi were analyzed using HPLC,and chromatographic fingerprints were established.The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Edition)was employed for common peak calibration and similarity evaluation.Additionally,principal component analysis was performed on the common peak area data.[Results]An HPLC fingerprint of L.foenum-graecum was developed,identifying a total of 13 common peaks.Among these,four characteristic components were specifically identified:chlorogenic acid,myricetin,quercetin,and kaempferol.The kaempferol chromatographic peak,exhibiting good resolution and a stable peak shape,was selected as the reference peak.The similarity indices between the fingerprints of the 10 sample batches and the reference fingerprint ranged from 0.954 to 0.995,indicating a relatively high consistency in the chemical composition of L.foenum-graecum from different origins.Principal component analysis identified two principal components,which together accounted for 89.45%of the cumulative variance,effectively capturing the primary chemical differences among the samples.[Conclusions]The established HPLC fingerprint method is straightforward to implement,stable,reliable,and exhibits high specificity.When combined with similarity evaluation and principal component analysis,it offers a scientific basis for developing quality standards for L.foenum-graecum medicinal materials.
基金Supported by Scientific Research Project of the Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine(GXZYA20220105).
文摘[Objectives]To evaluate the in vitro antibacterial,antioxidant,andα-glucosidase inhibitory activities of the ethanol total extract and four different polarity fractions(n-butanol,ethyl acetate,petroleum ether,and water)of Pilea peltata Hance,so as to provide a reference for its further development and research.[Methods]The antibacterial activity of P.peltata was evaluated in vitro by determining the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of its ethanol total extract and four different polarity fractions against seven test bacterial strains using the broth microdilution method.The in vitro antioxidant activity was investigated through DPPH radical,hydroxyl radical,and superoxide anion radical scavenging assays,with vitamin C(Vit C)as the positive control and the half maximal scavenging concentration(IC 50)as the evaluation indicator.The in vitroα-glucosidase inhibitory activity was assessed by measuring the peak area of p-nitrophenol(PNP),the hydrolysis product of 4-nitrophenylα-D-glucopyranoside(PNPG),via high-performance liquid chromatography(HPLC),using the half maximal inhibitory concentration(IC_(50))as the evaluation indicator.[Results]Both the ethanol total extract and the four different polarity fractions of P.peltata exhibited significant in vitro anti-Streptococcus pneumoniae activity.The DPPH radical scavenging capacities of the ethanol total extract and the various fractions were all weaker than that of VitC,with the order of efficacy being:n-butanol fraction>ethanol total extract>ethyl acetate fraction>petroleum ether fraction>aqueous fraction.For hydroxyl radical scavenging activity,the efficacy order of P.peltata fractions was:n-butanol extract>ethyl acetate extract>ethanol total extract>petroleum ether extract>aqueous extract.Notably,the n-butanol fraction(IC_(50)=0.068±0.001)demonstrated stronger activity than VitC(IC_(50)=0.097±0.001).The activity of the ethyl acetate fraction(IC_(50)=0.096±0.004)was comparable to that of VitC(IC_(50)=0.097±0.001).The superoxide anion scavenging capacities of the ethanol total extract and different polarity fractions from P.peltata were all weaker than that of VitC,with the order of efficacy being:n-butanol fraction>ethyl acetate fraction>ethanol total extract>petroleum ether fraction>aqueous fraction.The ethanol total extract and aqueous fraction of Pilea peltata showed no significant in vitroα-glucosidase inhibitory activity.Compared with the acarbose group,the IC 50 values of the ethyl acetate fraction and the n-butanol fraction both showed highly significant differences(P<0.01).[Conclusions]This study provides an experimental basis for the pharmacodynamic study and active component study of P.peltata.
基金The National Key Technology R&D Program"New Drug Innovation"of China(Grant No.2012ZX09301002-002-002,2012ZX09304-005)special funds for scientific research on traditional Chinese medicine(Grant No.201307002)National Science Fund for Excellent Young Scholars(Grant No.81222051)
文摘Abstract： To investigate the chemical constituents of the roots of Polygala wattersii Hance, the separation and purification were performed by solvent extraction and repeated column chromatography （CC） on silica gel, Sephadex LH-20 and macroporous resin D101, preparative TLC and semi-preparative HPLC. The structures were identified by spectroscopic analysis and comparison of their 1H and 13C NMR data with those reported in literatures. Twenty-three known compounds, including eleven xanthones （1-11）, nine sugar esters （12-20）, two triterpenoid saponins （21 and 22） and one phenylpropanoid （23） were isolated and their structures were identified as 1,3-dihydroxyxanthone （1）, 1-hydroxy-3-methoxyxanthone （2）, 1,3-dihydroxy-2-methoxyxanthone （3）, 1,3,7-trihydroxy-2- methoxyxanthone （4）, 1,3,6-trihydroxy-2,7-dimethoxyxanthone （5）, 1,6,7-trihydroxy-2,3-dimethoxyxanthone （6）, 1,7-dihydroxy- 2,3-methylenedioxyxanthone （7）, 1,7-dimethoxyxanthone （8）, 1,2,3-trimethoxyxanthone （9）, 1-methoxy-2,3-methylenedioxyxanthone （10）, 6-hydroxy-0-methoxy-2,3-methylenedioxyxanthone （11）, 3＇-O-feruloyl-6-O-acetyl sucrose （12）, arillatose B （13）, sibricose A5 （14）, sibricose A6 （15）, 3＇,6-di-O-sinapoyl sucrose （16）, tenufoliside A （17）, 3＇-O-3,4,5-trimethoxycinnamoyl-6-O-p-methoxybenzoyl sucrose （18）, glomeratose A （19）, 1-O-p-coumaroyl-D-glucopyranose （20）, bayogenin-3-O-glucoside （21）, tenufolin （22）, and sinapic acid （23）. Among them, compounds 2 and 12 were obtained from genus Polygala for the first time, and except compound 16, all others were isolated from this species for the first time.
基金Supported by Research Fund of the Doctoral Program of Higher Education (200805720004)Scientific Research Foundation for Returned Scholars, Ministry of Education of China ([2009]1001)~~
文摘[Objective] To construct prokaryotic expression vectors encoding gene Erb3binding protein （EBP1）, which plays important roles in regulating plant organ size from Nervilia fordii （Hance） Schltr. [Methods] PCR products of NfEBP1 with particular restriction sites and expression vectors, pET-28 and pET-16b were digested. Ligation, transformation and selection were performed to construct the recombinant plasmids pET-28-NfEBP1 and pET-16-NfEBP1. The recombinant plasmids were transformed into E. coli BL21 using heat -shock transformation. [Results] Recombinant plasmids pET-28-NfEBP1-1188 and pET-16-NfEBP1-1188 were constructed and transformed into expressional host cells, E. coli BL21, and validated by colony PCR, sequencing and double digestion. [Conclusion] Prokaryotic expression vectors of EBP1 gene from N. fordii were successfully constructed, which laid the foundation for characterization of the gene function.
基金Supported by the Research Funds of Jiangsu Open University/the City Vocational College of Jiangsu during the 12th Five-Year Plan Period(12SEW-Y-026)~~
文摘In the present study, we optimized the procedures to pretreat the parental material, to collect, sterilize and inoculate the explants in the tissue culture of Ar- disia mamillata Hance, so that more effective sterile explants can be obtained. The results revealed the optimal procedures. In detail, the parental materials were trans- ferred and pretreated in laboratory in February. The stem tips of new branches were collected in middle March, cleaned with eradicator and then rinsed with tap water for 1-2 h. After that, the explants were surface sterilized with 70% alcohol for 30 s, rinsed with sterile water 2-3 times, sterilized with 0.1% mercuric chloride for 4 min, rinsed with sterile water three times and sterilized with 0.1% mercuric chlo- ride for 4 min again. Finally, they were inoculated into medium after being rinsed with sterile water 5-8 times. By this method, the pollution rate of explants can be greatly reduced while their survival rate can be significantly improved.
基金supported by the National Natural Science Foundation of China (Nos.41977274, 41807123)the Shaanxi Province Key Research & Development Plan (No.2018ZDXMSF-022)the Scientific Research Program Funded by Shaanxi Provincial Education Department (No.18JK0100).
文摘Phytoremediation is a cost-effective and environment-friendly strategy for decontaminating heavy-metal-contaminated soil.However, the practical use of phytoremediation is constrained by the low biomass of plants and low bioavailability of heavy metals in soil.A pot experiment was conducted to investigate the effects of the metal chelator ethylenediaminetetraacetic acid(EDTA) and EDTA in combination with plant growth-promoting rhizobacteria(Burkholderia sp.D54 or Burkholderia sp.D416) on the growth and metal uptake of the hyperaccumulator Sedum alfredii Hance.According to the results, EDTA application decreased shoot and root biomass by 50% and 43%, respectively.The soil respiration and Cd,Pb, Zn uptake were depressed, while the photosynthetic rate, glutathione and phytochelatin(PC) contents were increased by EDTA application.Interestingly, Burkholderia sp.D54 and Burkholderia sp.D416 inoculation significantly relieved the inhibitory effects of EDTA on plant growth and soil respiration.Compared with the control, EDTA + D416 treatment increased the Cd concentration in shoots and decreased the Pb concentration in shoots and roots, but did not change the Zn concentration in S.alfredii plants.Furthermore,EDTA, EDTA + D54 and EDTA + D416 application increased the cysteine and PC contents in S.alfredii(p < 0.05);among all tested PCs, the most abundant species was PC2, and compared with the control, the PC2 content was increased by 371.0%, 1158.6% and 815.6%,respectively.These results will provide some insights into the practical use of EDTA and PGPR in the phytoremediation of heavy-metal-contaminated soil by S.alfredii.
基金Project supported by the National Natural Science Foundation of China (No. 20277035)the National Key Basic Research Program (973 Program) of China (No. 2002CB410804).
文摘Zn accumulation and subcellular distribution in leaves of the hyperaccumulating ecotype （HE） and non-hyperaccumulating ecotype （NHE） of Sedum alfredii Hance were studied using radiotracer and gradient centrifugation techniques. Leaf Zn accumulation in the HE of S. alfredii was 18.5-26.7 times greater than that in the NHE when the plants were grown at 1-500μmol Zn L-1. Leaf section uptake of 65Zn was highly dependent on external Zn levels. Greater 65Zn uptake in HE was noted only at external Zn levels 〉 100μmol L-1. Zinc subcellular distribution in the leaves of the two ecotypes of S. alfredii was： cell wall 〉 soluble fraction 〉 cell organelle. However, more Zn was distributed to the leaf cell wall and soluble fractions for HE than for NHE. In the leaf of HE, 91%-94% of the Zn was found in the cell walls and the soluble fraction and only 6%-9% Zn was distributed in the cell organelle fraction. For NHE, about 20%-26% Zn was recovered in the cell organelle fraction. In stems, Zn distribution to the ceil wail fraction was approximately two fold greater in the HE than that in the NHE. For the hyperaccumulating ecotype of S. alfredii, the cell wall and the vacuole played a very important role in Zn tolerance and hyperaccumulation.
基金supported by the key project supported of Hainan Province(Grant No.ZDZX2013008)Natural Science Foundation of China(Grant No.2014 81403006)
文摘Objective:To study the effect of Alpinia officinarum Hance(A.officinarum) 80% alcohol extract on the primary dysmenorrhea.Methods:A.officinarum 80% alcohol extract were enriched by macroporous adsorption resins.Female mice of primary dysmenorrhea model were established by oxytocin induction; the effects of A.officinarum 80% alcohol extract on primary dysmenorrhea were observed by body twist method; and the homogenate level of prostaglandin F_(2α)(PGF_(2α)),prostaglandin E_2(PGE_2) and Ca^(2+) in the uterus were observed in oxytocin-induced female mice.Results:The writhing frequency of primary dysmenorrhea mice was significantly decreased after treatment of A.officinarum 80% alcohol extract and the level of PGF_(2α),PGE_2 and Ca^(2+) in mice uterus was significantly decreased(P<0.05,P<0.01) in groups of mice treated with middle and high dosage of A.officinarum 80% alcohol extract compared with that of model group.Conclusions:These findings suggest that A.Officinarum 80% alcohol extract can significantly relieve primary dysmenorrhea.
文摘Two new phloroglucinol glycosides, lysidiciside A (1) and lysidiciside B (2), were isolated from the roots of Lysidice rhodostega Hance. Their structures were determined as 1-[(3-methylbutyryl)phloroglucinol]-β-D- glucopyranoside (1), 1- [(3-methylbutyryl)phloro- glucinol]-β-D-glucopyranosyl-5-O-β-D-glucopyranoside (2) on the basis of chemical and spectral analysis .
