In order to develop halophilic microorganism resources to improve environment, a Gram-positive, strictly aerobic and moderately halophilic bacterial strain JSA1 was obtained from the waste water sample collected from ...In order to develop halophilic microorganism resources to improve environment, a Gram-positive, strictly aerobic and moderately halophilic bacterial strain JSA1 was obtained from the waste water sample collected from Jinhong Chemical Plant at Weihai city, by the methods of quick isolation and screening of halophilie bacteria. Systematic studies on it were carried out. Results show that the strain JSA1 is bacillus. The temperature range most suitable for its growth is 29 - 35 ℃ and the most suitable pH is 6. 5 - 9. 0. It can grow well at the salt mass concentration of 30 - 150 g/L. The C ＋ G mole fraction of its DNA is 37.5%. The analytical result of 16S rRNA gene sequence reveals that this strain has the closest relationship with Alkalibacillus halophilus （DQ359731） of Alkalibacillts. Their similarities are as high as 99%. However, they have obvious differences in aspects of whole-cell main fatty acid components, cell size, cell morphology, motility, oxidase, gelatine liquefication, NaCl tolerance range, pH tolerance range, G ＋ C mole fraction, sole carbon source, sole nitrogen source, antibiotic sensitivity and strain source. Comparing with other species of the same genus, differences of this strain are even more obvious. In view of muhiple identification results, we believe this strain is a new subspecies ofAlkalibacillus halophilus and name it Alkalibacillus halophilus subsp, hitensis subsp, nov.展开更多
Carboxylic biomarkers in Saline lake sediments from the Jianghan basin(Hubei Province, China) and the Ejinor hypersaline lake (northern China) are studied. The origin, (?)ransformstion, and psleoecological significanc...Carboxylic biomarkers in Saline lake sediments from the Jianghan basin(Hubei Province, China) and the Ejinor hypersaline lake (northern China) are studied. The origin, (?)ransformstion, and psleoecological significances of isoprenoid, triterpenoid and alkenoic acids in the extracted organic matter (EOM) are also discussed.An i-C_(17:6) isoprenoid fatty scid and two C_(31:0) homogammscersnoic fatty acid stereoisomers are detected in psleossline lake sediments. Homogammaceranoic acid might be an important precursor of gammscerane which is abundant in saline sediments. The i-C_(17:6) isoprenoid acid and homogammacersnoic acid might be the lipid components of some halophilic microorganisms such as archaeobacteria halophilus.展开更多
基金Sponsored by the Science Foundation of Harbin Institute of Technology(Grant No. HIT(WH)XB200804)the National Basic Research Program of China(973 Program)(Grant No. 2004CB185050)
文摘In order to develop halophilic microorganism resources to improve environment, a Gram-positive, strictly aerobic and moderately halophilic bacterial strain JSA1 was obtained from the waste water sample collected from Jinhong Chemical Plant at Weihai city, by the methods of quick isolation and screening of halophilie bacteria. Systematic studies on it were carried out. Results show that the strain JSA1 is bacillus. The temperature range most suitable for its growth is 29 - 35 ℃ and the most suitable pH is 6. 5 - 9. 0. It can grow well at the salt mass concentration of 30 - 150 g/L. The C ＋ G mole fraction of its DNA is 37.5%. The analytical result of 16S rRNA gene sequence reveals that this strain has the closest relationship with Alkalibacillus halophilus （DQ359731） of Alkalibacillts. Their similarities are as high as 99%. However, they have obvious differences in aspects of whole-cell main fatty acid components, cell size, cell morphology, motility, oxidase, gelatine liquefication, NaCl tolerance range, pH tolerance range, G ＋ C mole fraction, sole carbon source, sole nitrogen source, antibiotic sensitivity and strain source. Comparing with other species of the same genus, differences of this strain are even more obvious. In view of muhiple identification results, we believe this strain is a new subspecies ofAlkalibacillus halophilus and name it Alkalibacillus halophilus subsp, hitensis subsp, nov.
基金This work was financially supported by the Org. Geochem. Lab., Academia Sinica, No. OGL-85-01.
文摘Carboxylic biomarkers in Saline lake sediments from the Jianghan basin(Hubei Province, China) and the Ejinor hypersaline lake (northern China) are studied. The origin, (?)ransformstion, and psleoecological significances of isoprenoid, triterpenoid and alkenoic acids in the extracted organic matter (EOM) are also discussed.An i-C_(17:6) isoprenoid fatty scid and two C_(31:0) homogammscersnoic fatty acid stereoisomers are detected in psleossline lake sediments. Homogammaceranoic acid might be an important precursor of gammscerane which is abundant in saline sediments. The i-C_(17:6) isoprenoid acid and homogammacersnoic acid might be the lipid components of some halophilic microorganisms such as archaeobacteria halophilus.
