In order to develop halophilic microorganism resources to improve environment, a Gram-positive, strictly aerobic and moderately halophilic bacterial strain JSA1 was obtained from the waste water sample collected from ...In order to develop halophilic microorganism resources to improve environment, a Gram-positive, strictly aerobic and moderately halophilic bacterial strain JSA1 was obtained from the waste water sample collected from Jinhong Chemical Plant at Weihai city, by the methods of quick isolation and screening of halophilie bacteria. Systematic studies on it were carried out. Results show that the strain JSA1 is bacillus. The temperature range most suitable for its growth is 29 - 35 ℃ and the most suitable pH is 6. 5 - 9. 0. It can grow well at the salt mass concentration of 30 - 150 g/L. The C ＋ G mole fraction of its DNA is 37.5%. The analytical result of 16S rRNA gene sequence reveals that this strain has the closest relationship with Alkalibacillus halophilus （DQ359731） of Alkalibacillts. Their similarities are as high as 99%. However, they have obvious differences in aspects of whole-cell main fatty acid components, cell size, cell morphology, motility, oxidase, gelatine liquefication, NaCl tolerance range, pH tolerance range, G ＋ C mole fraction, sole carbon source, sole nitrogen source, antibiotic sensitivity and strain source. Comparing with other species of the same genus, differences of this strain are even more obvious. In view of muhiple identification results, we believe this strain is a new subspecies ofAlkalibacillus halophilus and name it Alkalibacillus halophilus subsp, hitensis subsp, nov.展开更多
Biofilm formation of functional microorganisms in fermented foods can serve as a strategy to cope with environmental stress.Tetragenococcus halophilus,a key functional microorganism in traditional fermented foods,form...Biofilm formation of functional microorganisms in fermented foods can serve as a strategy to cope with environmental stress.Tetragenococcus halophilus,a key functional microorganism in traditional fermented foods,forms biofilms that aid in adapting to extreme conditions,thereby improving fermentation efficiency and product flavor.However,the regulation and molecular mechanisms of T.halophilus biofilms remain poorly studied.Hence,we investigated T.halophilus JY1 biofilms via dynamic monitoring,SEM,FT-IR,and transcriptomics,while verifying related targets in detail to determine the formation mechanism and stress response.Biofilm formation is a cyclical process with dynamic changes,and the demand for its remodelling is increased under high salinity conditions.Compared to planktonic state,T.halophilus JY1 biofilm cells showed enhanced extracellular polymer abundance and maintained intact cellular morphology with 2.5 M NaCl.Regarding gene expression,biofilm cells boosted glycolysis for energy accumulation and regulated carbon metabolic flow.Up-regulated expression of the dhaKLM gene cluster led to accumulated glucose,a precursor of extracellular polysaccharides,facilitating biofilm formation.This study addresses biofilm regulation from the viewpoint of extracellular polysaccharides and provides scientific evidence for resistance mechanisms in fermentation-functional microorganisms.展开更多
This study employed metagenomics and metabolomics to evaluate the impact of Tetragenococcus halophilus and Zygosaccharomyces rouxii on microbiota composition and metabolite profiles in secondary fortified fermented so...This study employed metagenomics and metabolomics to evaluate the impact of Tetragenococcus halophilus and Zygosaccharomyces rouxii on microbiota composition and metabolite profiles in secondary fortified fermented soy sauce.The Co-culturing of T.halophilus T10 and Z.rouxii QH-1(T1Z)enriched microbial diversity and promoted the proliferation of functional species,thereby enhancing biomarker species.Annotations matched from EggNOG,KEGG,and CAZy databases underscored the significance of carbohydrate and amino acid metabolism in moromi.Sample T1Z enhanced the metabolism by increasing the abundance and catalytic efficiency of the relevant enzymes,resulting in increases of 3.94%,2.69%,and 11.14%,respectively,with total nitrogen,amino acid nitrogen,and free amino acids.It introduced 11 volatile compounds and increased the alcohol content by 13.65%,and the aldehyde content by 49.28%,while also suppressing the accumulation of ammonium nitrogen and biogenic amines.Metabolic pathway analysis identified Staphylococcus gallinarum,Mammaliicoccus sciuri,and certain Bacillus species,especially B.parathracis,B.velezensis,and B.subtilis,as principal contributors to car-bohydrate and amino acid metabolism.These findings offer insight into microbial strain selection for industrial fermentation and provide a foundation for optimizing the process through co-culture bioaugmentation to enhance and underscore the potential of modern microbial techniques in traditional practices.展开更多
Carboxylic biomarkers in Saline lake sediments from the Jianghan basin(Hubei Province, China) and the Ejinor hypersaline lake (northern China) are studied. The origin, (?)ransformstion, and psleoecological significanc...Carboxylic biomarkers in Saline lake sediments from the Jianghan basin(Hubei Province, China) and the Ejinor hypersaline lake (northern China) are studied. The origin, (?)ransformstion, and psleoecological significances of isoprenoid, triterpenoid and alkenoic acids in the extracted organic matter (EOM) are also discussed.An i-C_(17:6) isoprenoid fatty scid and two C_(31:0) homogammscersnoic fatty acid stereoisomers are detected in psleossline lake sediments. Homogammaceranoic acid might be an important precursor of gammscerane which is abundant in saline sediments. The i-C_(17:6) isoprenoid acid and homogammacersnoic acid might be the lipid components of some halophilic microorganisms such as archaeobacteria halophilus.展开更多
基金Sponsored by the Science Foundation of Harbin Institute of Technology(Grant No. HIT(WH)XB200804)the National Basic Research Program of China(973 Program)(Grant No. 2004CB185050)
文摘In order to develop halophilic microorganism resources to improve environment, a Gram-positive, strictly aerobic and moderately halophilic bacterial strain JSA1 was obtained from the waste water sample collected from Jinhong Chemical Plant at Weihai city, by the methods of quick isolation and screening of halophilie bacteria. Systematic studies on it were carried out. Results show that the strain JSA1 is bacillus. The temperature range most suitable for its growth is 29 - 35 ℃ and the most suitable pH is 6. 5 - 9. 0. It can grow well at the salt mass concentration of 30 - 150 g/L. The C ＋ G mole fraction of its DNA is 37.5%. The analytical result of 16S rRNA gene sequence reveals that this strain has the closest relationship with Alkalibacillus halophilus （DQ359731） of Alkalibacillts. Their similarities are as high as 99%. However, they have obvious differences in aspects of whole-cell main fatty acid components, cell size, cell morphology, motility, oxidase, gelatine liquefication, NaCl tolerance range, pH tolerance range, G ＋ C mole fraction, sole carbon source, sole nitrogen source, antibiotic sensitivity and strain source. Comparing with other species of the same genus, differences of this strain are even more obvious. In view of muhiple identification results, we believe this strain is a new subspecies ofAlkalibacillus halophilus and name it Alkalibacillus halophilus subsp, hitensis subsp, nov.
基金was supported by Natural Science Foundation of Guangdong Province,China(2024A1515012719).
文摘Biofilm formation of functional microorganisms in fermented foods can serve as a strategy to cope with environmental stress.Tetragenococcus halophilus,a key functional microorganism in traditional fermented foods,forms biofilms that aid in adapting to extreme conditions,thereby improving fermentation efficiency and product flavor.However,the regulation and molecular mechanisms of T.halophilus biofilms remain poorly studied.Hence,we investigated T.halophilus JY1 biofilms via dynamic monitoring,SEM,FT-IR,and transcriptomics,while verifying related targets in detail to determine the formation mechanism and stress response.Biofilm formation is a cyclical process with dynamic changes,and the demand for its remodelling is increased under high salinity conditions.Compared to planktonic state,T.halophilus JY1 biofilm cells showed enhanced extracellular polymer abundance and maintained intact cellular morphology with 2.5 M NaCl.Regarding gene expression,biofilm cells boosted glycolysis for energy accumulation and regulated carbon metabolic flow.Up-regulated expression of the dhaKLM gene cluster led to accumulated glucose,a precursor of extracellular polysaccharides,facilitating biofilm formation.This study addresses biofilm regulation from the viewpoint of extracellular polysaccharides and provides scientific evidence for resistance mechanisms in fermentation-functional microorganisms.
基金supported by the Science and Technology Department of Sichuan Province of China(No:23JYC0056).
文摘This study employed metagenomics and metabolomics to evaluate the impact of Tetragenococcus halophilus and Zygosaccharomyces rouxii on microbiota composition and metabolite profiles in secondary fortified fermented soy sauce.The Co-culturing of T.halophilus T10 and Z.rouxii QH-1(T1Z)enriched microbial diversity and promoted the proliferation of functional species,thereby enhancing biomarker species.Annotations matched from EggNOG,KEGG,and CAZy databases underscored the significance of carbohydrate and amino acid metabolism in moromi.Sample T1Z enhanced the metabolism by increasing the abundance and catalytic efficiency of the relevant enzymes,resulting in increases of 3.94%,2.69%,and 11.14%,respectively,with total nitrogen,amino acid nitrogen,and free amino acids.It introduced 11 volatile compounds and increased the alcohol content by 13.65%,and the aldehyde content by 49.28%,while also suppressing the accumulation of ammonium nitrogen and biogenic amines.Metabolic pathway analysis identified Staphylococcus gallinarum,Mammaliicoccus sciuri,and certain Bacillus species,especially B.parathracis,B.velezensis,and B.subtilis,as principal contributors to car-bohydrate and amino acid metabolism.These findings offer insight into microbial strain selection for industrial fermentation and provide a foundation for optimizing the process through co-culture bioaugmentation to enhance and underscore the potential of modern microbial techniques in traditional practices.
基金This work was financially supported by the Org. Geochem. Lab., Academia Sinica, No. OGL-85-01.
文摘Carboxylic biomarkers in Saline lake sediments from the Jianghan basin(Hubei Province, China) and the Ejinor hypersaline lake (northern China) are studied. The origin, (?)ransformstion, and psleoecological significances of isoprenoid, triterpenoid and alkenoic acids in the extracted organic matter (EOM) are also discussed.An i-C_(17:6) isoprenoid fatty scid and two C_(31:0) homogammscersnoic fatty acid stereoisomers are detected in psleossline lake sediments. Homogammaceranoic acid might be an important precursor of gammscerane which is abundant in saline sediments. The i-C_(17:6) isoprenoid acid and homogammacersnoic acid might be the lipid components of some halophilic microorganisms such as archaeobacteria halophilus.
