A cell line, SHK, was derived from the kidney of spotted halibut Verasper variegates. The cell line was subcultured more than 40 passages in minimum essential medium （MEM） supplemented with fetal bovine serum （FBS...A cell line, SHK, was derived from the kidney of spotted halibut Verasper variegates. The cell line was subcultured more than 40 passages in minimum essential medium （MEM） supplemented with fetal bovine serum （FBS） and 10 ng ml4 basic fibroblast growth factor （bFGF）. Cell morphology from primary culture and subculture was observed continuously by microscopy. The SHK cell line consisted predominantly of fibroblast-like cells. The cell line was able to grow between 20℃ and 30℃ with the optimum growth at 24℃ and with a reduced growth between 12℃ and 20℃. The growth rate of the cells increased as the proportion of FBS increased from 10% to 20% at 28℃ with optimum growth at the concentration of 20%. The doubling time of the cells was determined to be 44.8 h. Chromosome analysis revealed that 52% of the SHK cells maintained a normal diploid chromosome number （2n=46）. The cells were successfully transfected with green fluorescent protein （GFP） reporter plasmids and the expression of GFP gene in the cells indicated the possible utility of the cells in gene expression studies. The cells were infected by lymphosystis disease virus （LCDV） and found to be susceptible to the virus in cytopathic effect （CPE） observation. The infection was confirmed by PCR and electron microscopy experiments, which proved the existence of the viral particles in the cytoplasm of the virus-infected cells.展开更多
This study was conducted to compare lipid and fatty acid composition of cod,haddock and halibut.Three groups of cod（276 g±61 g）,haddock（538 g±83 g）and halibut（3704 g±221 g）were maintained with com...This study was conducted to compare lipid and fatty acid composition of cod,haddock and halibut.Three groups of cod（276 g±61 g）,haddock（538 g±83 g）and halibut（3704 g±221 g）were maintained with commercial feeds mainly based on fish meal and marine fish oil for 12 weeks prior to sampling.The fatty acid compositions of muscle and liver were determined by GC/FID after derivatization of extracted lipids into fatty acid methyl esters（FAME）.Lipids were also fractionated into neutral and polar lipids using Waters silica Sep-Pak（R）.The phospholipid fraction was further separated by high-performance thin-layer chromatography（HPTLC）and the FAME profile was obtained.Results of the present study showed that cod and haddock were lean fish and their total muscle lipid contents were 0.8% and 0.7%,respectively,with phospholipid constituting 83.6% and 87.5% of the total muscle lipid,respectively.Halibut was a medium-fat fish and its muscle lipid content was 8%,with 84% of the total muscle lipid being neutral lipid.Total liver lipid contents of cod,haddock and halibut were 36.9%,67.2% and 30.7%,respectively,of which the neutral lipids accounted for the major fraction（88.1%-97.1%）.Polyunsaturated fatty acids were the most abundant in cod and haddock muscle neutral lipid.Monounsaturated fatty acid level was the highest in halibut muscle neutral lipid.Fatty acid compositions of phospholipid were relatively constant.In summary,the liver of cod and haddock as lean fish was the main lipid reserve organ,and structural phospholipid is the major lipid form in flesh.However,as a medium-fat fish,halibut stored lipid in both their liver and muscle.展开更多
Fish byproducts are potential feedstocks to extract valuable ingredients for human health while also helping to reduce environmental pollution.The present study investigated the hepatoprotective effects of refined Gre...Fish byproducts are potential feedstocks to extract valuable ingredients for human health while also helping to reduce environmental pollution.The present study investigated the hepatoprotective effects of refined Greenland halibut(Reinhardtius hippoglossoides)fin oil(RGO)on carbon tetrachloride(CCl4)-induced liver injury in mice.The RGO met the standard oil index of CODEX STAN 329-2017 and consisted of 69% monounsaturated fatty acids(MUFA),besides 10.19% ω-3 polyunsaturated fatty acid(ω-3 PUFA).The tube feeding of MUFA-enriched RGO fish oil significantly reduced liver damage parameters in the serum of CCl4-induced mice after 12 weeks and suppressed the expression of extracellular matrix(ECM),α-smooth muscle actin(α-SMA),and p-Smad2/3 by histological examination.RGO suppressed the indicators of hepatic stellate cells(HSCs)on transforming growth factor β1(TGF-β1)and α-SMA in the liver and decreased the level of Smad4 metabolite of the TGF-β/Smad pathway.The inhibitory effect of RGO on liver fibrosis was demonstrated by both in vivo and in vitro analysis.In this work,the refined Greenland halibut byproduct RGO has been used for the first time to decrease liver fibrosis in animal models of the disease.RGO can be added as a functional food element and presents a promising alternative to MUFA-enriched fish oil for liver disease control by preventing liver fibrosis.展开更多
基金supported by grants from State 863High-Technology Rand Project of China(2006AA09Z406,2006AA10A401)Taishan Scholar Project of Shan-dong Province
文摘A cell line, SHK, was derived from the kidney of spotted halibut Verasper variegates. The cell line was subcultured more than 40 passages in minimum essential medium （MEM） supplemented with fetal bovine serum （FBS） and 10 ng ml4 basic fibroblast growth factor （bFGF）. Cell morphology from primary culture and subculture was observed continuously by microscopy. The SHK cell line consisted predominantly of fibroblast-like cells. The cell line was able to grow between 20℃ and 30℃ with the optimum growth at 24℃ and with a reduced growth between 12℃ and 20℃. The growth rate of the cells increased as the proportion of FBS increased from 10% to 20% at 28℃ with optimum growth at the concentration of 20%. The doubling time of the cells was determined to be 44.8 h. Chromosome analysis revealed that 52% of the SHK cells maintained a normal diploid chromosome number （2n=46）. The cells were successfully transfected with green fluorescent protein （GFP） reporter plasmids and the expression of GFP gene in the cells indicated the possible utility of the cells in gene expression studies. The cells were infected by lymphosystis disease virus （LCDV） and found to be susceptible to the virus in cytopathic effect （CPE） observation. The infection was confirmed by PCR and electron microscopy experiments, which proved the existence of the viral particles in the cytoplasm of the virus-infected cells.
文摘This study was conducted to compare lipid and fatty acid composition of cod,haddock and halibut.Three groups of cod（276 g±61 g）,haddock（538 g±83 g）and halibut（3704 g±221 g）were maintained with commercial feeds mainly based on fish meal and marine fish oil for 12 weeks prior to sampling.The fatty acid compositions of muscle and liver were determined by GC/FID after derivatization of extracted lipids into fatty acid methyl esters（FAME）.Lipids were also fractionated into neutral and polar lipids using Waters silica Sep-Pak（R）.The phospholipid fraction was further separated by high-performance thin-layer chromatography（HPTLC）and the FAME profile was obtained.Results of the present study showed that cod and haddock were lean fish and their total muscle lipid contents were 0.8% and 0.7%,respectively,with phospholipid constituting 83.6% and 87.5% of the total muscle lipid,respectively.Halibut was a medium-fat fish and its muscle lipid content was 8%,with 84% of the total muscle lipid being neutral lipid.Total liver lipid contents of cod,haddock and halibut were 36.9%,67.2% and 30.7%,respectively,of which the neutral lipids accounted for the major fraction（88.1%-97.1%）.Polyunsaturated fatty acids were the most abundant in cod and haddock muscle neutral lipid.Monounsaturated fatty acid level was the highest in halibut muscle neutral lipid.Fatty acid compositions of phospholipid were relatively constant.In summary,the liver of cod and haddock as lean fish was the main lipid reserve organ,and structural phospholipid is the major lipid form in flesh.However,as a medium-fat fish,halibut stored lipid in both their liver and muscle.
文摘Fish byproducts are potential feedstocks to extract valuable ingredients for human health while also helping to reduce environmental pollution.The present study investigated the hepatoprotective effects of refined Greenland halibut(Reinhardtius hippoglossoides)fin oil(RGO)on carbon tetrachloride(CCl4)-induced liver injury in mice.The RGO met the standard oil index of CODEX STAN 329-2017 and consisted of 69% monounsaturated fatty acids(MUFA),besides 10.19% ω-3 polyunsaturated fatty acid(ω-3 PUFA).The tube feeding of MUFA-enriched RGO fish oil significantly reduced liver damage parameters in the serum of CCl4-induced mice after 12 weeks and suppressed the expression of extracellular matrix(ECM),α-smooth muscle actin(α-SMA),and p-Smad2/3 by histological examination.RGO suppressed the indicators of hepatic stellate cells(HSCs)on transforming growth factor β1(TGF-β1)and α-SMA in the liver and decreased the level of Smad4 metabolite of the TGF-β/Smad pathway.The inhibitory effect of RGO on liver fibrosis was demonstrated by both in vivo and in vitro analysis.In this work,the refined Greenland halibut byproduct RGO has been used for the first time to decrease liver fibrosis in animal models of the disease.RGO can be added as a functional food element and presents a promising alternative to MUFA-enriched fish oil for liver disease control by preventing liver fibrosis.
