人偏肺病毒(human metapneumovirus,hMPV)作为一种感染性病毒病原体,可导致严重的呼吸道感染,被世界卫生组织列为重要病原体之一。尽管疫苗在疾病预防与传播控制中发挥了重要作用,但由于hMPV的结构蛋白具有相对较高的突变率,野生型病毒...人偏肺病毒(human metapneumovirus,hMPV)作为一种感染性病毒病原体,可导致严重的呼吸道感染,被世界卫生组织列为重要病原体之一。尽管疫苗在疾病预防与传播控制中发挥了重要作用,但由于hMPV的结构蛋白具有相对较高的突变率,野生型病毒通常易于发生免疫逃逸。因此,设计能针对多种病毒变异株并为人体提供广泛保护的广谱hMPV疫苗具有重要意义。本研究基于共识序列方法,设计了一种广谱T细胞表位疫苗,该疫苗由478个氨基酸残基组成,涵盖来自病毒融合蛋白、附着糖蛋白、基质蛋白及小疏水性蛋白的10个细胞毒性T细胞(cytotoxic T lymphocyte,CTL)表位和11个辅助性T细胞(helper T lymphocyte,HTL)表位。进一步的分析表明,该多表位疫苗无致敏性,且具有高人群覆盖率、强抗原性和免疫原性,以及适宜的物理化学特性和较高的溶解性。同时,疫苗的结构与天然病毒高度相似。结构生物学分析表明,构建的疫苗在结构紧凑性和结合稳定性方面表现出较强的性能。计算机模拟的免疫学分析表明,该疫苗能激发人体的免疫应答。综上所述,本研究设计的广谱hMPV疫苗可作为一种用于预防hMPV感染的优秀候选疫苗;研究采用的预测流程可用于高效筛选其他病原体的免疫表位。展开更多
自Vanden—Hoogen在5岁以下的婴幼儿血清中100％发现了人类偏肺病毒（human meta pneumovirus，hMPV）抗体以来，hMPV已被世界各国公认为一种易导致婴幼儿呼吸道感染的病毒。来自国外的报道认为，它在不明病原的婴幼儿呼吸道感染中约占1...自Vanden—Hoogen在5岁以下的婴幼儿血清中100％发现了人类偏肺病毒（human meta pneumovirus，hMPV）抗体以来，hMPV已被世界各国公认为一种易导致婴幼儿呼吸道感染的病毒。来自国外的报道认为，它在不明病原的婴幼儿呼吸道感染中约占1．5％～21％。朱汝南等报道2002～2003年在北京地区常见呼吸道病毒检测阴性的呼吸道感染的患儿标本中hMPV的阳性率为24．1％，曾玫等报道2004年8月至2005年1月的上海地区呼吸道感染患儿标本中hMPV占13．8％，说明在我国也存在hMPV的流行。展开更多
Human metapneumovirus(HMPV) infection is one of the leading causes of hospitalization in young children with acute respiratory illness. In this study, we prospectively collected respiratory tract samples from children...Human metapneumovirus(HMPV) infection is one of the leading causes of hospitalization in young children with acute respiratory illness. In this study, we prospectively collected respiratory tract samples from children who were hospitalized with acute lower respiratory tract infection in six hospitals in China from 2017 to 2019. HMPV was detected in 145 out of 2733 samples(5.3%) from the hospitalized children. The majority of HMPV-positive children were under the age of two(67.6%), with a median age of one year. HMPV can independently cause acute lower respiratory tract infection in young children, while all patients showed mild clinical symptoms. Of all the co-infected patients, HMPV was most commonly detected with enterovirus(EV) or rhinovirus(RhV)(38.0%),followed by respiratory syncytial virus(RSV)(32.0%). The highest detection rate occurred from March to May in both northern and southern China. Out of 145 HMPV positive samples, 48 were successfully typed, of which 36strains were subgrouped into subtypes A2c(75%), eight strains were included in subtype B1(16.7%), and four strains were included in subtype B2(8.3%). Moreover, 16 A2c strains contained 111-nucleotide duplications in the G gene. Twenty-seven complete HMPV genomes were successfully obtained, and 25(92.6%) strains belonged to subtype A2c, whereas one strain was included in subgroup B1 and another was included in subgroup B2. A total of 277 mutations were observed in the complete genomes of 25 A2c strains. All results presented here improve our understanding of clinical characteristics and molecular epidemiology of HMPV infection in children.展开更多
文摘人偏肺病毒(human metapneumovirus,hMPV)作为一种感染性病毒病原体,可导致严重的呼吸道感染,被世界卫生组织列为重要病原体之一。尽管疫苗在疾病预防与传播控制中发挥了重要作用,但由于hMPV的结构蛋白具有相对较高的突变率,野生型病毒通常易于发生免疫逃逸。因此,设计能针对多种病毒变异株并为人体提供广泛保护的广谱hMPV疫苗具有重要意义。本研究基于共识序列方法,设计了一种广谱T细胞表位疫苗,该疫苗由478个氨基酸残基组成,涵盖来自病毒融合蛋白、附着糖蛋白、基质蛋白及小疏水性蛋白的10个细胞毒性T细胞(cytotoxic T lymphocyte,CTL)表位和11个辅助性T细胞(helper T lymphocyte,HTL)表位。进一步的分析表明,该多表位疫苗无致敏性,且具有高人群覆盖率、强抗原性和免疫原性,以及适宜的物理化学特性和较高的溶解性。同时,疫苗的结构与天然病毒高度相似。结构生物学分析表明,构建的疫苗在结构紧凑性和结合稳定性方面表现出较强的性能。计算机模拟的免疫学分析表明,该疫苗能激发人体的免疫应答。综上所述,本研究设计的广谱hMPV疫苗可作为一种用于预防hMPV感染的优秀候选疫苗;研究采用的预测流程可用于高效筛选其他病原体的免疫表位。
文摘自Vanden—Hoogen在5岁以下的婴幼儿血清中100％发现了人类偏肺病毒（human meta pneumovirus，hMPV）抗体以来，hMPV已被世界各国公认为一种易导致婴幼儿呼吸道感染的病毒。来自国外的报道认为，它在不明病原的婴幼儿呼吸道感染中约占1．5％～21％。朱汝南等报道2002～2003年在北京地区常见呼吸道病毒检测阴性的呼吸道感染的患儿标本中hMPV的阳性率为24．1％，曾玫等报道2004年8月至2005年1月的上海地区呼吸道感染患儿标本中hMPV占13．8％，说明在我国也存在hMPV的流行。
基金funded by the National Natural Science Foundation of China(82172275)the CAMS Innovation Fund for Medical Sciences,China(CIFMS,2019-I2M-5-026)
文摘Human metapneumovirus(HMPV) infection is one of the leading causes of hospitalization in young children with acute respiratory illness. In this study, we prospectively collected respiratory tract samples from children who were hospitalized with acute lower respiratory tract infection in six hospitals in China from 2017 to 2019. HMPV was detected in 145 out of 2733 samples(5.3%) from the hospitalized children. The majority of HMPV-positive children were under the age of two(67.6%), with a median age of one year. HMPV can independently cause acute lower respiratory tract infection in young children, while all patients showed mild clinical symptoms. Of all the co-infected patients, HMPV was most commonly detected with enterovirus(EV) or rhinovirus(RhV)(38.0%),followed by respiratory syncytial virus(RSV)(32.0%). The highest detection rate occurred from March to May in both northern and southern China. Out of 145 HMPV positive samples, 48 were successfully typed, of which 36strains were subgrouped into subtypes A2c(75%), eight strains were included in subtype B1(16.7%), and four strains were included in subtype B2(8.3%). Moreover, 16 A2c strains contained 111-nucleotide duplications in the G gene. Twenty-seven complete HMPV genomes were successfully obtained, and 25(92.6%) strains belonged to subtype A2c, whereas one strain was included in subgroup B1 and another was included in subgroup B2. A total of 277 mutations were observed in the complete genomes of 25 A2c strains. All results presented here improve our understanding of clinical characteristics and molecular epidemiology of HMPV infection in children.
