Objective To explore the mechanism of Shuwei Decoction(SWD)on functional dyspepsia(FD)rats with Gan stagnation and Pi deficiency syndrome(GSPDS)basedonPI3K/AKTTsignalingpathwayyand the proliferation of Cajal interstit...Objective To explore the mechanism of Shuwei Decoction(SWD)on functional dyspepsia(FD)rats with Gan stagnation and Pi deficiency syndrome(GSPDS)basedonPI3K/AKTTsignalingpathwayyand the proliferation of Cajal interstitial cells(ICCs)in gastric antrum.Methods Forty SD rats were divided into the control group,model group,SWD group(7.56 g/kg),and Mosapride group(1.35×10-3 g/kg)using a random number table,with 10 rats in each group.Except those in the control group,FD rats with GSPDS in the remaining groups were established using an enhanced composite etiology model(encompassing chronic restraint stress,tail-clamping,excessive fatigue,and dietary disturbances).After 21 days of modeling,rats in the control and the model groups were given normal saline by gavage,rats in the SWD and Mosapride group were administered with the corresponding drugs by gavage for 14 days.Body weight changes,gastric emptying rate and small intestinal propulsive rate were detected.Behavioral changes were evaluated using the elevated plus-maze test.The pathological injury degrees of gastric antral tissue was observed by HE staining,and the protein expression levelsof phosphatidyli-nositol-3 kinase(PI3K),proteinkinase B(AKT),p-AKT,and anoctamin-1(ANO1)were detected by Western Blot.Additionally,the mRNA expression levels of PI3K,p-AKT,and ANO1 were detected in gastric sinus using RT-qPCR.The c-kit expression level was detected by immunofluorescence colocalization,while the proliferative activity of ICCs was evaluated with CCK8,along with apoptosis rate of ICCs was measured by flow cytometry.Results Compared with the'control group,the model group exhibited a decrease in body weight,gastric emptying rate,and small intestinal propulsive rate,as well as a decrease in proteins and mRNA expression levels of PI3K,p-AKT,ANO1(P<0.05),a decrease in the expression of c-kit and proliferative rate of ICCs(P<0.05),and an increase in level of apoptosis(P<0.05).Compared with the model group,the SWD and Mosapride groups showed an increase in body weight,gastric emptying rate,and small intestinal propulsive rate,an increase in proteins and mRNA expression levels of PI3K,p-AKT,ANO1(P<0.05),an increase in the expressions of c-kit and propulsive rate of ICCs(P<0.05),and a decrease in apoptosis levels(P<0.05).Conclusion SWD could enhance gastric emptying rate and small intestinal propulsive rate,reduce the apoptosis of ICCs and gastric sinusoidal cells,and improve the gastrointestinal motility in FD rats with GSPDS.Its mechanisms might be related to regulating ICCs via the PI3K/AKT signaling pathway.展开更多
文摘Objective To explore the mechanism of Shuwei Decoction(SWD)on functional dyspepsia(FD)rats with Gan stagnation and Pi deficiency syndrome(GSPDS)basedonPI3K/AKTTsignalingpathwayyand the proliferation of Cajal interstitial cells(ICCs)in gastric antrum.Methods Forty SD rats were divided into the control group,model group,SWD group(7.56 g/kg),and Mosapride group(1.35×10-3 g/kg)using a random number table,with 10 rats in each group.Except those in the control group,FD rats with GSPDS in the remaining groups were established using an enhanced composite etiology model(encompassing chronic restraint stress,tail-clamping,excessive fatigue,and dietary disturbances).After 21 days of modeling,rats in the control and the model groups were given normal saline by gavage,rats in the SWD and Mosapride group were administered with the corresponding drugs by gavage for 14 days.Body weight changes,gastric emptying rate and small intestinal propulsive rate were detected.Behavioral changes were evaluated using the elevated plus-maze test.The pathological injury degrees of gastric antral tissue was observed by HE staining,and the protein expression levelsof phosphatidyli-nositol-3 kinase(PI3K),proteinkinase B(AKT),p-AKT,and anoctamin-1(ANO1)were detected by Western Blot.Additionally,the mRNA expression levels of PI3K,p-AKT,and ANO1 were detected in gastric sinus using RT-qPCR.The c-kit expression level was detected by immunofluorescence colocalization,while the proliferative activity of ICCs was evaluated with CCK8,along with apoptosis rate of ICCs was measured by flow cytometry.Results Compared with the'control group,the model group exhibited a decrease in body weight,gastric emptying rate,and small intestinal propulsive rate,as well as a decrease in proteins and mRNA expression levels of PI3K,p-AKT,ANO1(P<0.05),a decrease in the expression of c-kit and proliferative rate of ICCs(P<0.05),and an increase in level of apoptosis(P<0.05).Compared with the model group,the SWD and Mosapride groups showed an increase in body weight,gastric emptying rate,and small intestinal propulsive rate,an increase in proteins and mRNA expression levels of PI3K,p-AKT,ANO1(P<0.05),an increase in the expressions of c-kit and propulsive rate of ICCs(P<0.05),and a decrease in apoptosis levels(P<0.05).Conclusion SWD could enhance gastric emptying rate and small intestinal propulsive rate,reduce the apoptosis of ICCs and gastric sinusoidal cells,and improve the gastrointestinal motility in FD rats with GSPDS.Its mechanisms might be related to regulating ICCs via the PI3K/AKT signaling pathway.
