Objective To investigate the effect of Qingda Granules(QDG)on renal artery fibrosis induced by hypertension.Methods Six WKY rats were selected to serve as the control group.Totally 30 spontaneously hypertensive rats(S...Objective To investigate the effect of Qingda Granules(QDG)on renal artery fibrosis induced by hypertension.Methods Six WKY rats were selected to serve as the control group.Totally 30 spontaneously hypertensive rats(SHRs)were randomly divided into the model group,low-dose QDG group,medium-dose QDG group,high-dose QDG group,and the positive drug group,with six rats in each group.The QDG low,medium,and high dose groups were administered with QDG intragastrically at doses of 450,900,and 1800 mg·kg*1.d-1,respectively,while the positive drug group was given valsartan intragastrically at a dose of 7.2 mg:kg-1.d-1.Both the control and model groups were intragastrically administered with an equivalent volume of double-distilled water,once daily for 10 consecutive weeks.HE staining was used to detect the pathological changes of renal artery tissue.RNA sequencing was used to analyze differentially expressed transcripts,and Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)were used to analyze the potential pathways.Angiotensin II(Ang II)stimulation was used to establish a model of rat renal artery vascular smooth muscle cells to verify the effects of different doses of QDG on fibrosis and activation of potential enrichment pathways.Results QDG significantly reduced the thickness of renal artery media in SHR rats(P<0.05).RNA sequencing analysis indicated that 1423 dfferential transcripts changed after QDG intervention.GO analysis showed that cellular processes and biological processes were significantly enriched.KEGG analysis found that mitogen-activated protein kinase(MAPK),transforming growth factorβ(TGF-β)and other pathways were significantly enriched.QDG significantly inhibited the protein expressions of Collagen I,Collagen II,matrix metalloproteinase 9(MMP-9),p-Smad2,p-Smad3,and TGF-β1 in rat renal artery vascular smooth muscle cells stimulated byAngg II,and increased the protein expressions of tissue inhibitor of metalloproteinase(TIMP)1 and TIMP2(P<0.05).Conclusion QDG inhibits the activation of TGFβ-1/Smad2/3 pathway to reduce renal artery fibrosis induced by hypertension.展开更多
文摘Objective To investigate the effect of Qingda Granules(QDG)on renal artery fibrosis induced by hypertension.Methods Six WKY rats were selected to serve as the control group.Totally 30 spontaneously hypertensive rats(SHRs)were randomly divided into the model group,low-dose QDG group,medium-dose QDG group,high-dose QDG group,and the positive drug group,with six rats in each group.The QDG low,medium,and high dose groups were administered with QDG intragastrically at doses of 450,900,and 1800 mg·kg*1.d-1,respectively,while the positive drug group was given valsartan intragastrically at a dose of 7.2 mg:kg-1.d-1.Both the control and model groups were intragastrically administered with an equivalent volume of double-distilled water,once daily for 10 consecutive weeks.HE staining was used to detect the pathological changes of renal artery tissue.RNA sequencing was used to analyze differentially expressed transcripts,and Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)were used to analyze the potential pathways.Angiotensin II(Ang II)stimulation was used to establish a model of rat renal artery vascular smooth muscle cells to verify the effects of different doses of QDG on fibrosis and activation of potential enrichment pathways.Results QDG significantly reduced the thickness of renal artery media in SHR rats(P<0.05).RNA sequencing analysis indicated that 1423 dfferential transcripts changed after QDG intervention.GO analysis showed that cellular processes and biological processes were significantly enriched.KEGG analysis found that mitogen-activated protein kinase(MAPK),transforming growth factorβ(TGF-β)and other pathways were significantly enriched.QDG significantly inhibited the protein expressions of Collagen I,Collagen II,matrix metalloproteinase 9(MMP-9),p-Smad2,p-Smad3,and TGF-β1 in rat renal artery vascular smooth muscle cells stimulated byAngg II,and increased the protein expressions of tissue inhibitor of metalloproteinase(TIMP)1 and TIMP2(P<0.05).Conclusion QDG inhibits the activation of TGFβ-1/Smad2/3 pathway to reduce renal artery fibrosis induced by hypertension.
