Studies were conducted under pot conditions to determine the comparative efficacy ofcarbofuran at 1 mg a.i./kg soil, bavistin at 1 mg a.i./kg soil, neem （Azadirachta indica） seed powder at 50 mg/kg soil, green mould...Studies were conducted under pot conditions to determine the comparative efficacy ofcarbofuran at 1 mg a.i./kg soil, bavistin at 1 mg a.i./kg soil, neem （Azadirachta indica） seed powder at 50 mg/kg soil, green mould （Trichoderma harzianum） at 50.0 ml/kg soil, rhizobacteria （Pseudomonas fluorescens） at 50.0 ml/kg soil against root-knot nematode, Meloidogyne incognita-wilt fungus, Fusarium oxysporum disease complex on green gram, Vigna radiata cv ML-1108. All the treatments significantly improved the growth of the plants as compared carbofuran and A. indica seed powder increased plant growth to untreated inoculated plants. Analysis of data showed that and yield significantly more in comparison to bavistin and P. fluorescens. Carbofuran was highly effective against nematode, bavistin against fungus, A. indica seed powder against both the pathogens and both the bioagents were moderately effective against both the pathogens.展开更多
Black gram (Vigna mungo L. Hepper) is one of the main leguminous crops that provide chief source of food. Several Bradyrhizobium species are able to induce effective nodules in black gram cultivars. In the present stu...Black gram (Vigna mungo L. Hepper) is one of the main leguminous crops that provide chief source of food. Several Bradyrhizobium species are able to induce effective nodules in black gram cultivars. In the present study, we characterized forty isolates of indigenous black gram bradyrhizobia from Myanmar based on the sequence analysis of the bacterial 16S rRNA gene. The sequence analysis confirmed that all isolates were categorized and identified as the genus Bradyrhizobium and they were conspecific with B. elkanii, B. sp., B. liaoningense, B. japonicum and B. yunamingense. Almost all the collected isolates from major black gram growing regions of Nyaunglebin Bago Regio, Chaungzon Mon State, Sittwe Rakhine State, Danubyu Ayeyarwady Region and Launglon Tanintharyi Region were identified as B. liaoningense. At Danubyu Ayeyarwady Region and Pyinmanar Nay Pyi Taw Region, most of the strains were identified as B. japonicum. On the other hand, more or less all the isolates from Launglon Tanintharyi Region and Hpa-an Kayin State were related to B. elkanii. However, all B. sp. strains were found in Salingyi Sagaing Region black gram growing region. This is the first report describing Bradyrhizobium strains that were isolated from soil samples of major black gram growing areas in Myanmar. Evaluation of the effectiveness of Myanmar Bradyrhizobim strains isolated from soil samples of major black gram growing areas of Myanmar for plant growth and nitrogen fixation was studied in pot experiments with completely randomized design and three replicates. The nodule dry weight, shoot dry weight and acetylene reduction activity of the plant inoculated with Bradyrhizobium elkanii LauBG38 were significantly higher in ARA per plant, nodule and shoot dry weights than the other tested isolates in both Yezin-4 and Yezin-7 black gram varieties. We expect that Myanmar Bradyrhizobium elkanii LauBG38 will be able to use as Biofertilizer for black gram cultivars.展开更多
【目的】GRAM(Glucosyltransferases,Rab-like GTPase activators and Myotubularins)是普遍存在于动、植物蛋白中的结构域,在植物生长发育及响应逆境胁迫等过程中发挥着重要功能。在马铃薯全基因组中鉴定GRAM基因家族成员,分析马铃薯G...【目的】GRAM(Glucosyltransferases,Rab-like GTPase activators and Myotubularins)是普遍存在于动、植物蛋白中的结构域,在植物生长发育及响应逆境胁迫等过程中发挥着重要功能。在马铃薯全基因组中鉴定GRAM基因家族成员,分析马铃薯GRAM家族基因在盐胁迫下的表达模式,探究GRAM家族在马铃薯盐胁迫过程中的作用。【方法】采用生物信息学方法鉴定马铃薯中GRAM家族成员,并对蛋白理化性质、染色体定位、亚细胞定位、基因结构、motif及共线性等方面进行分析。利用转录组测序和荧光定量PCR(RT-qPCR)对该家族成员在盐胁迫下的表达模式进行研究。【结果】在马铃薯中共鉴定到26个GRAM家族基因,不均匀地分布于7条染色体上;理化性质分析显示StGRAM全部为亲水性蛋白,大部分为碱性蛋白;亚细胞定位预测StGRAM蛋白大部分存在于叶绿体和细胞核;根据系统进化分析可将StGRAM家族分为3个亚族,同一亚族成员具有相似的基因结构及motif分布;通过马铃薯物种内共线性分析发现StGRAM仅有一对同源基因,物种间共线性显示StGRAM在水稻和拟南芥中分别存在5对和3对同源基因;在StGRAM基因启动子区发现大量的激素响应元件和逆境胁迫响应元件;转录组测序分析和RT-qPCR分析显示,StGRAM基因受盐胁迫的诱导表达,可能参与了马铃薯对盐胁迫的响应过程,StGRAM25基因可能对中性盐和碱性盐有不同的响应模式。【结论】StGRAM基因家族在马铃薯盐胁迫响应和信号转导过程中发挥着重要作用。展开更多
Green gram(Vigna radiata(L.)wilczek,)commonly known as moong or mung bean or golden gram was introduced to Eritrea as a pulse crop by Ministry of Agriculture at its National Agricultural Research Institute(NARI)in col...Green gram(Vigna radiata(L.)wilczek,)commonly known as moong or mung bean or golden gram was introduced to Eritrea as a pulse crop by Ministry of Agriculture at its National Agricultural Research Institute(NARI)in collaboration with Association for Strengthening Agricultural Research in Eastern and Central Africa(ASARECA)in 2012.But its agronomic practices for semi-arid conditions of Eritrea are not yet standardised.Therefore,to find the optimum inter row spacing and phosphorus dose for its higher productivity,a field experiment was conducted at the experimental farm of Hamelmalo Agricultural College,Keren,Eritrea during summer 2015 and 2016.The experiment was conducted in randomised complete block design(RCBD)with 12 treatment combinations of two factors consisting of four inter row spacing(Broadcast,18 cm,30 cm,and 45 cm)and three phosphorus levels(0,20 and 40 kg P2O5 ha^(-1))each replicated thrice.The results of the study revealed that sowing of K-26 bold seeded variety either by broadcast method or at 18 cm inter row spacing at10 cm plant to plant spacing fertilized with 40 kg P2O5 ha-1 through DAP fertilizer drilled at the time of sowing proved significantly superior to increase growth,yield attributes and seed yield of green gram.展开更多
随着能源行业的快速发展和技术革新,大量的专业术语和表达方式不断更新,新词不断涌现。然而,传统的新词发现方法通常依赖于词典或规则,且难以高效率地处理和更新大量的专业术语,特别是在快速变化的能源领域。因此,结合能源领域文本数据...随着能源行业的快速发展和技术革新,大量的专业术语和表达方式不断更新,新词不断涌现。然而,传统的新词发现方法通常依赖于词典或规则,且难以高效率地处理和更新大量的专业术语,特别是在快速变化的能源领域。因此,结合能源领域文本数据特性,提出了一种融合N-Gram和多重注意力机制的能源领域新词发现方法(new word discovery method in the energy field combining N-Gram and multiple attention mechanism, ENFM)。该方法首先利用N-Gram模型对能源领域的文本数据进行初步处理,通过统计和分析词频来生成新词候选列表。随后,引入融合多重注意力机制的ERNIE-BiLSTM-CRF模型,以进一步提升新词发现的准确性和效率。与传统的新词发现技术相比,在新词的准确识别和整体效率上均有显著提升,将其于能源领域政策文本数据集,准确率、召回率和F1分别为95.71%、95.56%、95.63%。实验结果表明,该方法能够准确地在能源领域的大量文本数据中识别新词,有效识别出能源领域特有的词汇和表达方式,显著提高了中文分词任务中对能源领域专业术语的识别能力。展开更多
文摘Studies were conducted under pot conditions to determine the comparative efficacy ofcarbofuran at 1 mg a.i./kg soil, bavistin at 1 mg a.i./kg soil, neem （Azadirachta indica） seed powder at 50 mg/kg soil, green mould （Trichoderma harzianum） at 50.0 ml/kg soil, rhizobacteria （Pseudomonas fluorescens） at 50.0 ml/kg soil against root-knot nematode, Meloidogyne incognita-wilt fungus, Fusarium oxysporum disease complex on green gram, Vigna radiata cv ML-1108. All the treatments significantly improved the growth of the plants as compared carbofuran and A. indica seed powder increased plant growth to untreated inoculated plants. Analysis of data showed that and yield significantly more in comparison to bavistin and P. fluorescens. Carbofuran was highly effective against nematode, bavistin against fungus, A. indica seed powder against both the pathogens and both the bioagents were moderately effective against both the pathogens.
文摘Black gram (Vigna mungo L. Hepper) is one of the main leguminous crops that provide chief source of food. Several Bradyrhizobium species are able to induce effective nodules in black gram cultivars. In the present study, we characterized forty isolates of indigenous black gram bradyrhizobia from Myanmar based on the sequence analysis of the bacterial 16S rRNA gene. The sequence analysis confirmed that all isolates were categorized and identified as the genus Bradyrhizobium and they were conspecific with B. elkanii, B. sp., B. liaoningense, B. japonicum and B. yunamingense. Almost all the collected isolates from major black gram growing regions of Nyaunglebin Bago Regio, Chaungzon Mon State, Sittwe Rakhine State, Danubyu Ayeyarwady Region and Launglon Tanintharyi Region were identified as B. liaoningense. At Danubyu Ayeyarwady Region and Pyinmanar Nay Pyi Taw Region, most of the strains were identified as B. japonicum. On the other hand, more or less all the isolates from Launglon Tanintharyi Region and Hpa-an Kayin State were related to B. elkanii. However, all B. sp. strains were found in Salingyi Sagaing Region black gram growing region. This is the first report describing Bradyrhizobium strains that were isolated from soil samples of major black gram growing areas in Myanmar. Evaluation of the effectiveness of Myanmar Bradyrhizobim strains isolated from soil samples of major black gram growing areas of Myanmar for plant growth and nitrogen fixation was studied in pot experiments with completely randomized design and three replicates. The nodule dry weight, shoot dry weight and acetylene reduction activity of the plant inoculated with Bradyrhizobium elkanii LauBG38 were significantly higher in ARA per plant, nodule and shoot dry weights than the other tested isolates in both Yezin-4 and Yezin-7 black gram varieties. We expect that Myanmar Bradyrhizobium elkanii LauBG38 will be able to use as Biofertilizer for black gram cultivars.
文摘【目的】GRAM(Glucosyltransferases,Rab-like GTPase activators and Myotubularins)是普遍存在于动、植物蛋白中的结构域,在植物生长发育及响应逆境胁迫等过程中发挥着重要功能。在马铃薯全基因组中鉴定GRAM基因家族成员,分析马铃薯GRAM家族基因在盐胁迫下的表达模式,探究GRAM家族在马铃薯盐胁迫过程中的作用。【方法】采用生物信息学方法鉴定马铃薯中GRAM家族成员,并对蛋白理化性质、染色体定位、亚细胞定位、基因结构、motif及共线性等方面进行分析。利用转录组测序和荧光定量PCR(RT-qPCR)对该家族成员在盐胁迫下的表达模式进行研究。【结果】在马铃薯中共鉴定到26个GRAM家族基因,不均匀地分布于7条染色体上;理化性质分析显示StGRAM全部为亲水性蛋白,大部分为碱性蛋白;亚细胞定位预测StGRAM蛋白大部分存在于叶绿体和细胞核;根据系统进化分析可将StGRAM家族分为3个亚族,同一亚族成员具有相似的基因结构及motif分布;通过马铃薯物种内共线性分析发现StGRAM仅有一对同源基因,物种间共线性显示StGRAM在水稻和拟南芥中分别存在5对和3对同源基因;在StGRAM基因启动子区发现大量的激素响应元件和逆境胁迫响应元件;转录组测序分析和RT-qPCR分析显示,StGRAM基因受盐胁迫的诱导表达,可能参与了马铃薯对盐胁迫的响应过程,StGRAM25基因可能对中性盐和碱性盐有不同的响应模式。【结论】StGRAM基因家族在马铃薯盐胁迫响应和信号转导过程中发挥着重要作用。
文摘Green gram(Vigna radiata(L.)wilczek,)commonly known as moong or mung bean or golden gram was introduced to Eritrea as a pulse crop by Ministry of Agriculture at its National Agricultural Research Institute(NARI)in collaboration with Association for Strengthening Agricultural Research in Eastern and Central Africa(ASARECA)in 2012.But its agronomic practices for semi-arid conditions of Eritrea are not yet standardised.Therefore,to find the optimum inter row spacing and phosphorus dose for its higher productivity,a field experiment was conducted at the experimental farm of Hamelmalo Agricultural College,Keren,Eritrea during summer 2015 and 2016.The experiment was conducted in randomised complete block design(RCBD)with 12 treatment combinations of two factors consisting of four inter row spacing(Broadcast,18 cm,30 cm,and 45 cm)and three phosphorus levels(0,20 and 40 kg P2O5 ha^(-1))each replicated thrice.The results of the study revealed that sowing of K-26 bold seeded variety either by broadcast method or at 18 cm inter row spacing at10 cm plant to plant spacing fertilized with 40 kg P2O5 ha-1 through DAP fertilizer drilled at the time of sowing proved significantly superior to increase growth,yield attributes and seed yield of green gram.
文摘随着能源行业的快速发展和技术革新,大量的专业术语和表达方式不断更新,新词不断涌现。然而,传统的新词发现方法通常依赖于词典或规则,且难以高效率地处理和更新大量的专业术语,特别是在快速变化的能源领域。因此,结合能源领域文本数据特性,提出了一种融合N-Gram和多重注意力机制的能源领域新词发现方法(new word discovery method in the energy field combining N-Gram and multiple attention mechanism, ENFM)。该方法首先利用N-Gram模型对能源领域的文本数据进行初步处理,通过统计和分析词频来生成新词候选列表。随后,引入融合多重注意力机制的ERNIE-BiLSTM-CRF模型,以进一步提升新词发现的准确性和效率。与传统的新词发现技术相比,在新词的准确识别和整体效率上均有显著提升,将其于能源领域政策文本数据集,准确率、召回率和F1分别为95.71%、95.56%、95.63%。实验结果表明,该方法能够准确地在能源领域的大量文本数据中识别新词,有效识别出能源领域特有的词汇和表达方式,显著提高了中文分词任务中对能源领域专业术语的识别能力。
