A protein conjugate of streptomycin （streptomycin-bovine serum albumin （BSA） conjugate） was prepared and used as immunogen to produce monoclonal antibodies （MAb）. One hybridoma secreting anti-streptomycin MAb wa...A protein conjugate of streptomycin （streptomycin-bovine serum albumin （BSA） conjugate） was prepared and used as immunogen to produce monoclonal antibodies （MAb）. One hybridoma secreting anti-streptomycin MAb was obtained and then used to produce MAb. The MAb named 13H5 showed the 50% maximal inhibitory concentra- tion （IC50） value of 4.65 ng/ml and the IC20value of 0.21 ng/ml in phosphate buffered saline （PBS）. At optimum con- ditions, an indirect competitive enzyme-linked immunosorbent assay （ELISA） and a colloidal gold-based immuno- chromatographic assay （CGIA） were developed and applied to detect streptomycin residues in milk and swine urine samples. The developed ELISA showed that the minimum detection limit was 2.0 and 1.9 ng/ml for milk and swine urine samples, respectively, without obvious cross-reactivity to other tested antibiotics except dihydrostreptomycin which gave a 118.32% cross reaction value. Milk and swine urine samples spiked with streptomycin at 10, 50, 100 and 200 ng/rnl were analyzed by the established ELISA. The mean recovery of streptomycin was from 81.9% to 105.5% and from 84.3% to 92.2% for milk and swine urine, respectively. The optimized CGIA showed that the minimum de- tection limit was 20.0 ng/ml for milk and swine urine samples. The results of spiked analysis and specific analysis demonstrate that the CGIA could be applicable for screening milk and swine urine samples for the presence of streptomycin residues on-site. The established ELISA and CGIA allow the rapid, low-cost, and sensitive determination of streptomycin residues in food samples.展开更多
The activation of HCl by cationic Au in the presence of C2H2 is important for the construction of active Au sites and in acetylene hydrochlorination.Here,we report a strategy for activating HCl by the Au-based support...The activation of HCl by cationic Au in the presence of C2H2 is important for the construction of active Au sites and in acetylene hydrochlorination.Here,we report a strategy for activating HCl by the Au-based supported ionic liquid phase(Au–SILP)technology with the[N(CN)2^–]anion.This strategy enables HCl to accept electrons from[N(CN)2^–]anions in Au–[N(CN)2^–]complexes rather than from pure[Bmim][N(CN)2],leading to notable improvement in both the reaction path and the stability of the catalyst without changing the reaction triggered by acetylene adsorption.Furthermore,the induction period of the Au–SILP catalyst was shown to be absent in the reaction process due to the high Au(III)content in the Au(Ⅲ)/Au(Ⅰ)site and the high substrate diffusion rate in the ionic liquid layer.This work provides a facile method to improve the stability of Au-based catalysts for acetylene hydrochlorination.展开更多
Rice stripe virus(RSV) causes dramatic losses in rice production worldwide. In this study, two monoclonal antibodies(MAbs) 16E6 and 11 C1 against RSV and a colloidal gold-based immunochromatographic strip were develop...Rice stripe virus(RSV) causes dramatic losses in rice production worldwide. In this study, two monoclonal antibodies(MAbs) 16E6 and 11 C1 against RSV and a colloidal gold-based immunochromatographic strip were developed for specific, sensitive, and rapid detection of RSV in rice plant and planthopper samples. The MAb 16E6 was conjugated with colloidal gold and the MAb 11C1 was coated on the test line of the nitrocellulose membrane of the test strip. The specificity of the test strip was confirmed by a positive reaction to RSV-infected rice plants and small brown planthopper(SBPH), and negative reactions to five other rice viruses, healthy rice plants, four other vectors of five rice viruses, and non-viruliferous SBPH. Sensitivity analyses showed that the test strip could detect the virus in RSV-infected rice plant tissue crude extracts diluted to 1:20 480(w/v, g/mL), and in individual viruliferous SBPH homogenate diluted to 1:2560(individual SPBH/μL). The validity of the developed strip was further confirmed by tests using field-collected rice and SBPH samples. This newly developed test strip is a low-cost, fast, and easy-to-use tool for on-site detection of RSV infection during field epidemiological studies and paddy field surveys, and thus can benefit decision-making for RSV management in the field.展开更多
Brain cancer,also known as intracranial cancer,is one of the most invasive and fatal cancers affecting people of all ages.Despite the great advances in medical technology,improvements in transporting drugs into brain ...Brain cancer,also known as intracranial cancer,is one of the most invasive and fatal cancers affecting people of all ages.Despite the great advances in medical technology,improvements in transporting drugs into brain tissue have been limited by the challenge of crossing the blood-brain barrier(BBB).Fortunately,recent endeavors using gold-based nanomaterials(GBNs)have indicated the potential of these materials to cross the BBB.Therefore,GBNs might be an attractive therapeutic strategy against brain cancer.Herein,we aim to present a comprehensive summary of current understanding of the critical effects of the physicochemical properties and surface modifications of GBNs on BBB penetration for applications in brain cancer treatment.Furthermore,the most recent GBNs and their impressive performance in precise bioimaging and efficient inhibition of brain tumors are also summarized,with an emphasis on the mechanism of their effective BBB penetration.Finally,the challenges and future outlook in using GBNs for brain cancer treatment are discussed.We hope that this review will spark researchers'interest in constructing more powerful nanoplatforms for brain disease treatment.展开更多
Radionuclides have been widely used for multimodal imaging and radioisotope therapy of cancer.Various nanomaterials have been developed as excellent nanocarriers of radionuclides for the targeted delivery into tumors,...Radionuclides have been widely used for multimodal imaging and radioisotope therapy of cancer.Various nanomaterials have been developed as excellent nanocarriers of radionuclides for the targeted delivery into tumors,in order to minimize the unnecessary side effect and enhance the therapeutic efficacy of radiotherapy.Among those nanomaterials,gold nanomaterials with tunable morphologies,easy modification,good biological safety,and radiation sensitization capability are excellent candidates for cancer theranostics.Given the superior performance of gold-based nanomaterials in biomedicine,we summary the recent advance of radionuclide labeled/doped gold-based nanomaterials for cancer theranostics.In this review article,we will discuss the methods for labelling or doping radionuclides onto gold nanomaterials,their applications for nuclear imaging and Cerenkov luminescence(CL)imaging,as well as the radioisotope therapy of cancer,and finally the toxicity evaluation of radionuclide labeled/doped gold-based nanomaterials.We hope that our review article would provide guidance for non-experts to design the radiolabeled nanomaterials for cancer imaging guided therapy.展开更多
Introducing ligand into the surface of gold(Au)-based catalyst has been recognized as an efficient strategy to enhance the performance of catalyst in acetylene hydrochlorination reaction.However,due to the multifactor...Introducing ligand into the surface of gold(Au)-based catalyst has been recognized as an efficient strategy to enhance the performance of catalyst in acetylene hydrochlorination reaction.However,due to the multifactorial deactivation,the usage of single type of ligand has limitations on the performance improvement.In this work,two types of ligands including a molecular 2-methylimidazole and an ionic cetrimonium are selected to protect Au^(n+)species.After kinetics analysis,advanced characterization,and density functional theory simulation,we demonstrate the optimal interaction model between two ligands and Au species:Two 2-methylimidazole molecules are coordinated with high-valent Au species while cetrimonium is interacted via electrostatic interaction.Except the synergistic effect in the decrease of Au species reduction and agglomeration,the existence of molecular ligand greatly increases the adsorption of hydrogen chloride while the ionic ligand significantly inhibits the deposition of coke.Due to the positive effect of dual-ligands,we achieved 97.1%of acetylene conversion and 0.29 h^(−1) of deactivation rate under high gas hourly space velocity of acetylene.This work establishes a foundation to explore the property-activity relationships in Au-based catalyst via ligand engineering.展开更多
基金Project (No.2007C22047) supported by the Program of Science and Technology of Zhejiang Province,China
文摘A protein conjugate of streptomycin （streptomycin-bovine serum albumin （BSA） conjugate） was prepared and used as immunogen to produce monoclonal antibodies （MAb）. One hybridoma secreting anti-streptomycin MAb was obtained and then used to produce MAb. The MAb named 13H5 showed the 50% maximal inhibitory concentra- tion （IC50） value of 4.65 ng/ml and the IC20value of 0.21 ng/ml in phosphate buffered saline （PBS）. At optimum con- ditions, an indirect competitive enzyme-linked immunosorbent assay （ELISA） and a colloidal gold-based immuno- chromatographic assay （CGIA） were developed and applied to detect streptomycin residues in milk and swine urine samples. The developed ELISA showed that the minimum detection limit was 2.0 and 1.9 ng/ml for milk and swine urine samples, respectively, without obvious cross-reactivity to other tested antibiotics except dihydrostreptomycin which gave a 118.32% cross reaction value. Milk and swine urine samples spiked with streptomycin at 10, 50, 100 and 200 ng/rnl were analyzed by the established ELISA. The mean recovery of streptomycin was from 81.9% to 105.5% and from 84.3% to 92.2% for milk and swine urine, respectively. The optimized CGIA showed that the minimum de- tection limit was 20.0 ng/ml for milk and swine urine samples. The results of spiked analysis and specific analysis demonstrate that the CGIA could be applicable for screening milk and swine urine samples for the presence of streptomycin residues on-site. The established ELISA and CGIA allow the rapid, low-cost, and sensitive determination of streptomycin residues in food samples.
文摘The activation of HCl by cationic Au in the presence of C2H2 is important for the construction of active Au sites and in acetylene hydrochlorination.Here,we report a strategy for activating HCl by the Au-based supported ionic liquid phase(Au–SILP)technology with the[N(CN)2^–]anion.This strategy enables HCl to accept electrons from[N(CN)2^–]anions in Au–[N(CN)2^–]complexes rather than from pure[Bmim][N(CN)2],leading to notable improvement in both the reaction path and the stability of the catalyst without changing the reaction triggered by acetylene adsorption.Furthermore,the induction period of the Au–SILP catalyst was shown to be absent in the reaction process due to the high Au(III)content in the Au(Ⅲ)/Au(Ⅰ)site and the high substrate diffusion rate in the ionic liquid layer.This work provides a facile method to improve the stability of Au-based catalysts for acetylene hydrochlorination.
基金Project supported by the National Key Research and Development Program of China(No.2016YFD0300706)the Ministry of Agriculture of China(No.2016ZX08009003-001)+1 种基金the National Natural Science Foundation of China(No.31571976)the Earmarked Fund for China Agriculture Research System(No.nycytux-001)
文摘Rice stripe virus(RSV) causes dramatic losses in rice production worldwide. In this study, two monoclonal antibodies(MAbs) 16E6 and 11 C1 against RSV and a colloidal gold-based immunochromatographic strip were developed for specific, sensitive, and rapid detection of RSV in rice plant and planthopper samples. The MAb 16E6 was conjugated with colloidal gold and the MAb 11C1 was coated on the test line of the nitrocellulose membrane of the test strip. The specificity of the test strip was confirmed by a positive reaction to RSV-infected rice plants and small brown planthopper(SBPH), and negative reactions to five other rice viruses, healthy rice plants, four other vectors of five rice viruses, and non-viruliferous SBPH. Sensitivity analyses showed that the test strip could detect the virus in RSV-infected rice plant tissue crude extracts diluted to 1:20 480(w/v, g/mL), and in individual viruliferous SBPH homogenate diluted to 1:2560(individual SPBH/μL). The validity of the developed strip was further confirmed by tests using field-collected rice and SBPH samples. This newly developed test strip is a low-cost, fast, and easy-to-use tool for on-site detection of RSV infection during field epidemiological studies and paddy field surveys, and thus can benefit decision-making for RSV management in the field.
基金supported by the National Natural Science Foundation of China(NSFC)(Grant Nos.82001959 and 31630027)NSFC-German Research Foundation(DFG)project(Grant No.31761133013)+6 种基金appreciate support from the“Ten Thousand Elite Plan”(Grant No.Y9E21Z11)CAS International Collaboration Plan(Grant No.E0632911ZX)the National Key Research&Development Program of China(Grant No.2018YFE0117800)the Key Laboratory of Biomedical Effects of Nanomaterials and NanosafetyCAS(Grant No.NSKF202003)Fujian Provincial Key Laboratory of Innovative Drug Target Research(Grant No.FJ-YW-2021KF04)financial support from the Nanqiang Outstanding Young Talents Program from Xiamen University。
文摘Brain cancer,also known as intracranial cancer,is one of the most invasive and fatal cancers affecting people of all ages.Despite the great advances in medical technology,improvements in transporting drugs into brain tissue have been limited by the challenge of crossing the blood-brain barrier(BBB).Fortunately,recent endeavors using gold-based nanomaterials(GBNs)have indicated the potential of these materials to cross the BBB.Therefore,GBNs might be an attractive therapeutic strategy against brain cancer.Herein,we aim to present a comprehensive summary of current understanding of the critical effects of the physicochemical properties and surface modifications of GBNs on BBB penetration for applications in brain cancer treatment.Furthermore,the most recent GBNs and their impressive performance in precise bioimaging and efficient inhibition of brain tumors are also summarized,with an emphasis on the mechanism of their effective BBB penetration.Finally,the challenges and future outlook in using GBNs for brain cancer treatment are discussed.We hope that this review will spark researchers'interest in constructing more powerful nanoplatforms for brain disease treatment.
基金This work was partially supported by National Natural Science Foundation of China(31822022,U1932208,31900986)a Jiangsu Natural Science Fund for Outstanding Youth Science Foundation(BK20180094)+1 种基金the Foundation of Jiangsu Provincial Medical Innovation Team(CXTDA2017042)a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘Radionuclides have been widely used for multimodal imaging and radioisotope therapy of cancer.Various nanomaterials have been developed as excellent nanocarriers of radionuclides for the targeted delivery into tumors,in order to minimize the unnecessary side effect and enhance the therapeutic efficacy of radiotherapy.Among those nanomaterials,gold nanomaterials with tunable morphologies,easy modification,good biological safety,and radiation sensitization capability are excellent candidates for cancer theranostics.Given the superior performance of gold-based nanomaterials in biomedicine,we summary the recent advance of radionuclide labeled/doped gold-based nanomaterials for cancer theranostics.In this review article,we will discuss the methods for labelling or doping radionuclides onto gold nanomaterials,their applications for nuclear imaging and Cerenkov luminescence(CL)imaging,as well as the radioisotope therapy of cancer,and finally the toxicity evaluation of radionuclide labeled/doped gold-based nanomaterials.We hope that our review article would provide guidance for non-experts to design the radiolabeled nanomaterials for cancer imaging guided therapy.
基金supported by the National Natural Science Foundation of China(No.22068031)Yunnan Precious Metals Laboratory Science and Technology Project(No.YPML-2022050237)+4 种基金Major Science and Technology Project of Yunnan Precious Metal Laboratory(No.YPML-2023050202)the Science and Technology Project of Xinjiang Bingtuan supported by Central Government(No.2022BC001)Tianshan Talents Training Program of Xinjiang Science and Technology Innovation Team(No.2022TSYCTD0021)the Start-Up Foundation for Young Scientists of Shihezi University(No.RCZK202419)the Project of Achievement Transformation and Technology Extension of Shihezi University(No.CGZH202302)。
文摘Introducing ligand into the surface of gold(Au)-based catalyst has been recognized as an efficient strategy to enhance the performance of catalyst in acetylene hydrochlorination reaction.However,due to the multifactorial deactivation,the usage of single type of ligand has limitations on the performance improvement.In this work,two types of ligands including a molecular 2-methylimidazole and an ionic cetrimonium are selected to protect Au^(n+)species.After kinetics analysis,advanced characterization,and density functional theory simulation,we demonstrate the optimal interaction model between two ligands and Au species:Two 2-methylimidazole molecules are coordinated with high-valent Au species while cetrimonium is interacted via electrostatic interaction.Except the synergistic effect in the decrease of Au species reduction and agglomeration,the existence of molecular ligand greatly increases the adsorption of hydrogen chloride while the ionic ligand significantly inhibits the deposition of coke.Due to the positive effect of dual-ligands,we achieved 97.1%of acetylene conversion and 0.29 h^(−1) of deactivation rate under high gas hourly space velocity of acetylene.This work establishes a foundation to explore the property-activity relationships in Au-based catalyst via ligand engineering.
